Q96MI9 (CBPC4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic carboxypeptidase 4
EC=3.4.17.-

Alternative name(s):
ATP/GTP-binding protein-like 1

Gene names

Name:	AGBL1
Synonyms:	CCP4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1066 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5 By similarity.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Interacts with MYLK By similarity.
Subcellular location	Cytoplasm › cytosol By similarity.
Sequence similarities	Belongs to the peptidase M14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Metal-binding Zinc
Molecular function	Carboxypeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	C-terminal protein deglutamylation Inferred from sequence or structural similarity. Source: UniProtKB protein side chain deglutamylation Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metallocarboxypeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96MI9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q96MI9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-301: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q96MI9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-269: Missing.
     270-276: DTEDGKV → MGQCYNL

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1066	1066	Cytosolic carboxypeptidase 4		PRO_0000304999
Regions
	Compositional bias	251 – 284	34	Asp-rich
Sites
	Active site	808	1	Nucleophile By similarity
	Metal binding	758	1	Zinc By similarity
	Metal binding	761	1	Zinc By similarity
	Metal binding	855	1	Zinc By similarity
Natural variations
	Alternative sequence	1 – 301	301	Missing in isoform 2.		VSP_028176
	Alternative sequence	1 – 269	269	Missing in isoform 3.		VSP_028177
	Alternative sequence	270 – 276	7	DTEDGKV → MGQCYNL in isoform 3.		VSP_028178
	Natural variant	417	1	P → L. Corresponds to variant rs8029810 [ dbSNP | Ensembl ].		VAR_048604
	Natural variant	435	1	S → P. Corresponds to variant rs11857527 [ dbSNP | Ensembl ].		VAR_048605
	Natural variant	1010	1	Q → R. Corresponds to variant rs8028043 [ dbSNP | Ensembl ].		VAR_059195
Experimental info
	Sequence conflict	446	1	V → A in BAB71299. Ref.1
	Sequence conflict	524	1	S → G in BAB71299. Ref.1
	Sequence conflict	628	1	A → D in BAB71299. Ref.1
	Sequence conflict	658	1	G → R in AAI28153. Ref.3
	Sequence conflict	747	1	H → E in BAB71299. Ref.1
	Sequence conflict	747	1	H → E in AAI28153. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 2, 2007. Version 2. Checksum: FC08DC1C44991096	FASTA	1,066	120,281
	10 20 30 40 50 60 MISKGGSEAL LQTLVDTART APPDYDILLP LFRLLAKVGL RDKKIGRKAL ELEALDVTLI 70 80 90 100 110 120 LARKNLSHGQ NLLHCLWALR VFASSVSMGA MLGINGAMEL LFKVITPYTR KRTQAIRAAT 130 140 150 160 170 180 EVLAALLKSK SNGRRAVNRG YVTSLLGLHQ DWHSHDTANA YVQIRRGLLL CLRHIAALRS 190 200 210 220 230 240 GREAFLAAQG MEILFSTTQN CLDDKSMEPV ISVVLQILRQ CYPTSPLPLV TASSAYAFPV 250 260 270 280 290 300 PGCITTEPPH DLPEEDFEDD GDDEVDKDSD TEDGKVEDDD LETDVNKLSS KPGLDRPEEE 310 320 330 340 350 360 LMQYEVMCLE LSYSFEELQS KLGDDLNSEK TQYANHHHIP AAASSKQHCY SKDQSSCGQE 370 380 390 400 410 420 REYAVQTSLL CRVKTGRSTV HLGSKKNPGV NLYQNVQSNS LRRDSSESEI PDIQASPKAD 430 440 450 460 470 480 AWDVDAIFCP RMSASFSNST RTREVVKVID KLLQTHLKRV PFHDPYLYMA KARRTSSVVD 490 500 510 520 530 540 FKMMAFPDVW GHCPPPTTQP MLERKCGVQR IRIFEDIRRL IQPSDVINKV VFSLDEPWPL 550 560 570 580 590 600 QDNASNCLRF FSKFESGNLR KAIQVREFEY DLLVNADVNS TQHQQWFYFK VSGMQAAIPY 610 620 630 640 650 660 HFNIINCEKP NSQFNYGMQP TLYSVKEALL GKPTWIRTGH EICYYKNHYR QSTAVAGGAS 670 680 690 700 710 720 GKCYYTLTFA VTFPHSEDVC YLAYHYPYTY TALMTHLDIL EKSVNLKEVY FRQDVLCQTL 730 740 750 760 770 780 GGNPCPLVTI TAMPESNSDE HLEQFRHRPY QVITARVHPG ESNASWVMKG TLEFLVSSDP 790 800 810 820 830 840 VARLLRENFI FKIIPMLNPD GVINGNHRCS LSGEDLNRQW LSPSAHLQPT IYHAKGLLYH 850 860 870 880 890 900 LSSIGRSPVV FCDFHGHSQK KNVFLYGCSI KETLWQAACT VGTSTILEEV NYRTLPKILD 910 920 930 940 950 960 KLAPAFTMSS CSFLVEKSRA STARVVVWRE MGVSRSYTME SSYCGCNQGP YQCTQRLLER 970 980 990 1000 1010 1020 TKNERAHPVD GLQGLQFGTR ELEEMGAMFC LGLLILELKS ASCSHQLLAQ AATLLSAEED 1030 1040 1050 1060 ALDQHLQRLK SSNFLPKHIW FAYHFFAITN FFKMNLLLHV SPVCDT
	Isoform 2 [UniParc]. Checksum: 9E0A227F4533D78A	FASTA	765	87,153
	10 20 30 40 50 60 MQYEVMCLEL SYSFEELQSK LGDDLNSEKT QYANHHHIPA AASSKQHCYS KDQSSCGQER 70 80 90 100 110 120 EYAVQTSLLC RVKTGRSTVH LGSKKNPGVN LYQNVQSNSL RRDSSESEIP DIQASPKADA 130 140 150 160 170 180 WDVDAIFCPR MSASFSNSTR TREVVKVIDK LLQTHLKRVP FHDPYLYMAK ARRTSSVVDF 190 200 210 220 230 240 KMMAFPDVWG HCPPPTTQPM LERKCGVQRI RIFEDIRRLI QPSDVINKVV FSLDEPWPLQ 250 260 270 280 290 300 DNASNCLRFF SKFESGNLRK AIQVREFEYD LLVNADVNST QHQQWFYFKV SGMQAAIPYH 310 320 330 340 350 360 FNIINCEKPN SQFNYGMQPT LYSVKEALLG KPTWIRTGHE ICYYKNHYRQ STAVAGGASG 370 380 390 400 410 420 KCYYTLTFAV TFPHSEDVCY LAYHYPYTYT ALMTHLDILE KSVNLKEVYF RQDVLCQTLG 430 440 450 460 470 480 GNPCPLVTIT AMPESNSDEH LEQFRHRPYQ VITARVHPGE SNASWVMKGT LEFLVSSDPV 490 500 510 520 530 540 ARLLRENFIF KIIPMLNPDG VINGNHRCSL SGEDLNRQWL SPSAHLQPTI YHAKGLLYHL 550 560 570 580 590 600 SSIGRSPVVF CDFHGHSQKK NVFLYGCSIK ETLWQAACTV GTSTILEEVN YRTLPKILDK 610 620 630 640 650 660 LAPAFTMSSC SFLVEKSRAS TARVVVWREM GVSRSYTMES SYCGCNQGPY QCTQRLLERT 670 680 690 700 710 720 KNERAHPVDG LQGLQFGTRE LEEMGAMFCL GLLILELKSA SCSHQLLAQA ATLLSAEEDA 730 740 750 760 LDQHLQRLKS SNFLPKHIWF AYHFFAITNF FKMNLLLHVS PVCDT
	Isoform 3 [UniParc]. Checksum: 640C4FC2882C7DD0	FASTA	797	90,789
	10 20 30 40 50 60 MGQCYNLEDD DLETDVNKLS SKPGLDRPEE ELMQYEVMCL ELSYSFEELQ SKLGDDLNSE 70 80 90 100 110 120 KTQYANHHHI PAAASSKQHC YSKDQSSCGQ EREYAVQTSL LCRVKTGRST VHLGSKKNPG 130 140 150 160 170 180 VNLYQNVQSN SLRRDSSESE IPDIQASPKA DAWDVDAIFC PRMSASFSNS TRTREVVKVI 190 200 210 220 230 240 DKLLQTHLKR VPFHDPYLYM AKARRTSSVV DFKMMAFPDV WGHCPPPTTQ PMLERKCGVQ 250 260 270 280 290 300 RIRIFEDIRR LIQPSDVINK VVFSLDEPWP LQDNASNCLR FFSKFESGNL RKAIQVREFE 310 320 330 340 350 360 YDLLVNADVN STQHQQWFYF KVSGMQAAIP YHFNIINCEK PNSQFNYGMQ PTLYSVKEAL 370 380 390 400 410 420 LGKPTWIRTG HEICYYKNHY RQSTAVAGGA SGKCYYTLTF AVTFPHSEDV CYLAYHYPYT 430 440 450 460 470 480 YTALMTHLDI LEKSVNLKEV YFRQDVLCQT LGGNPCPLVT ITAMPESNSD EHLEQFRHRP 490 500 510 520 530 540 YQVITARVHP GESNASWVMK GTLEFLVSSD PVARLLRENF IFKIIPMLNP DGVINGNHRC 550 560 570 580 590 600 SLSGEDLNRQ WLSPSAHLQP TIYHAKGLLY HLSSIGRSPV VFCDFHGHSQ KKNVFLYGCS 610 620 630 640 650 660 IKETLWQAAC TVGTSTILEE VNYRTLPKIL DKLAPAFTMS SCSFLVEKSR ASTARVVVWR 670 680 690 700 710 720 EMGVSRSYTM ESSYCGCNQG PYQCTQRLLE RTKNERAHPV DGLQGLQFGT RELEEMGAMF 730 740 750 760 770 780 CLGLLILELK SASCSHQLLA QAATLLSAEE DALDQHLQRL KSSNFLPKHI WFAYHFFAIT 790 NFFKMNLLLH VSPVCDT

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Prostate.
[2]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed: 16572171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK056872 mRNA. Translation: BAB71299.1. AC016180 Genomic DNA. No translation available. AC107978 Genomic DNA. No translation available. AC025842 Genomic DNA. No translation available. AC069197 Genomic DNA. No translation available. AC022817 Genomic DNA. No translation available. AC012229 Genomic DNA. No translation available. AC018950 Genomic DNA. No translation available. AC105204 Genomic DNA. No translation available. AC078905 Genomic DNA. No translation available. BC128152 mRNA. Translation: AAI28153.1.
IPI	IPI00053232. IPI00783246. IPI00867537.
RefSeq	NP_689549.2. NM_152336.2.
UniGene	Hs.679833.
3D structure databases
ProteinModelPortal	Q96MI9.
ModBase	Search...
Polymorphism databases
DMDM	158706472.
Proteomic databases
PRIDE	Q96MI9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000299974; ENSP00000299974; ENSG00000166748. ENST00000389298; ENSP00000373949; ENSG00000166748. ENST00000421325; ENSP00000397173; ENSG00000166748.
GeneID	123624.
KEGG	hsa:123624.
UCSC	uc002blz.1. human.
Organism-specific databases
CTD	123624.
GeneCards	GC15P086685.
HGNC	HGNC:26504. AGBL1.
HPA	HPA039653.
neXtProt	NX_Q96MI9.
PharmGKB	PA134923894.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG10081.
GeneTree	ENSGT00550000074405.
HOGENOM	HBG447015.
HOVERGEN	HBG107587.
InParanoid	Q96MI9.
Gene expression databases
Bgee	Q96MI9.
CleanEx	HS_AGBL1.
Genevestigator	Q96MI9.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000834. Peptidase_M14. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit.
Pfam	PF00246. Peptidase_M14. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS00132. CARBOXYPEPT_ZN_1. False negative. PS00133. CARBOXYPEPT_ZN_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	81119.

Entry information

Entry name

CBPC4_HUMAN

Accession

Primary (citable) accession number: Q96MI9
Secondary accession number(s): A1A4X5, A6NJH6, C9JHL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.