Q96MI6 (PPM1M_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1M
EC=3.1.3.16

Alternative name(s):
Protein phosphatase 2C isoform eta
Short name=PP2C-eta
Short name=PP2CE

Gene names

Name:	PPM1M
Synonyms:	PPM1E

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate. UniProtKB Q8BU27
Cofactor	Magnesium or manganese Potential.
Subcellular location	Nucleus By similarity UniProtKB Q8BU27.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	CTD phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB manganese ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96MI6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q96MI6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     105-123: AFVYPELLAGEFTRLEFPR → VGALGSMEAVKLQLLGPGP
     124-270: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q96MI6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-129: Missing.
     130-144: LGQKVLFRDHHMSGW → MGHRAWVDAGSAPGR
     252-270: YCSCWGPAWAWVGASSKPK → FSKLAQMLIHSTQGKEDSLTEEGQVSYDDVSVFVIPLHSQGQESSDH

Note: No experimental confirmation available.

Isoform 4 (identifier: Q96MI6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.
     252-270: YCSCWGPAWAWVGASSKPK → FSKLAQMLIHSTQGKEDSLTEEGQVSYDDVSVFVIPLHSQGQESSDH

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Protein phosphatase 1M		PRO_0000057756
Regions
	Domain	1 – 192	192	PP2C-like
Sites
	Metal binding	223	1	Manganese Potential UniProtKB P35813
Natural variations
	Alternative sequence	1 – 129	129	Missing in isoform 3.		VSP_050660
	Alternative sequence	1 – 51	51	Missing in isoform 4.		VSP_050659
	Alternative sequence	105 – 123	19	AFVYP…LEFPR → VGALGSMEAVKLQLLGPGP in isoform 2.		VSP_050663
	Alternative sequence	124 – 270	147	Missing in isoform 2.		VSP_050664
	Alternative sequence	130 – 144	15	LGQKV…HMSGW → MGHRAWVDAGSAPGR in isoform 3.		VSP_050661
	Alternative sequence	252 – 270	19	YCSCW…SSKPK → FSKLAQMLIHSTQGKEDSLT EEGQVSYDDVSVFVIPLHSQ GQESSDH in isoform 3 and isoform 4.		VSP_050662

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: FCBE6EDF7C127144	FASTA	270	30,375
	10 20 30 40 50 60 MHLNGRCICP SDPQFVEEKG IRAEDLVIGA LESAFQECDE VIGRELEASG QMGGCTALVA 70 80 90 100 110 120 VSLQGKLYMA NAGDSRAILV RRDEIRPLSF EFTPETERQR IQQLAFVYPE LLAGEFTRLE 130 140 150 160 170 180 FPRRLKGDDL GQKVLFRDHH MSGWSYKRVE KSDLKYPLIH GQGRQARLLG TLAVSRGLGD 190 200 210 220 230 240 HQLRVLDTNI QLKPFLLSVP QVTVLDVDQL ELQEDDVVVM ATDGLWDVLS NEQVAWLVRS 250 260 270 FLPGNQEDPH RYCSCWGPAW AWVGASSKPK
	Isoform 2 [UniParc]. Checksum: 8821EC221BBEA624	FASTA	123	13,330
	10 20 30 40 50 60 MHLNGRCICP SDPQFVEEKG IRAEDLVIGA LESAFQECDE VIGRELEASG QMGGCTALVA 70 80 90 100 110 120 VSLQGKLYMA NAGDSRAILV RRDEIRPLSF EFTPETERQR IQQLVGALGS MEAVKLQLLG PGP
	Isoform 3 [UniParc]. Checksum: FCBC85A80A95B7A8	FASTA	169	18,837
	10 20 30 40 50 60 MGHRAWVDAG SAPGRSYKRV EKSDLKYPLI HGQGRQARLL GTLAVSRGLG DHQLRVLDTN 70 80 90 100 110 120 IQLKPFLLSV PQVTVLDVDQ LELQEDDVVV MATDGLWDVL SNEQVAWLVR SFLPGNQEDP 130 140 150 160 HRFSKLAQML IHSTQGKEDS LTEEGQVSYD DVSVFVIPLH SQGQESSDH
	Isoform 4 [UniParc]. Checksum: 4738FD6CBF4613BB	FASTA	247	27,923
	10 20 30 40 50 60 MGGCTALVAV SLQGKLYMAN AGDSRAILVR RDEIRPLSFE FTPETERQRI QQLAFVYPEL 70 80 90 100 110 120 LAGEFTRLEF PRRLKGDDLG QKVLFRDHHM SGWSYKRVEK SDLKYPLIHG QGRQARLLGT 130 140 150 160 170 180 LAVSRGLGDH QLRVLDTNIQ LKPFLLSVPQ VTVLDVDQLE LQEDDVVVMA TDGLWDVLSN 190 200 210 220 230 240 EQVAWLVRSF LPGNQEDPHR FSKLAQMLIH STQGKEDSLT EEGQVSYDDV SVFVIPLHSQ GQESSDH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Peripheral blood, Prostate and Thymus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Uterus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK056894 mRNA. Translation: BAB71302.1. AK096681 mRNA. Translation: BAC04839.1. AK129647 mRNA. Translation: BAC85206.1. BC009644 mRNA. Translation: AAH09644.1.
IPI	IPI00165163. IPI00167493. IPI00398744. IPI00942895.
RefSeq	NP_001116342.1. NM_001122870.2. NP_653242.3. NM_144641.3.
UniGene	Hs.373560. Hs.731914.
3D structure databases
ProteinModelPortal	Q96MI6.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000387046.
Polymorphism databases
DMDM	41688718.
Proteomic databases
PaxDb	Q96MI6.
PRIDE	Q96MI6.
Protocols and materials databases
DNASU	132160.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000296487; ENSP00000296487; ENSG00000164088. ENST00000409502; ENSP00000387046; ENSG00000164088.
GeneID	132160.
KEGG	hsa:132160.
UCSC	uc003ddf.4. human. uc003ddg.4. human. uc003ddh.4. human.
Organism-specific databases
CTD	132160.
GeneCards	GC03P052279.
HGNC	HGNC:26506. PPM1M.
HPA	HPA036905.
MIM	608979. gene.
neXtProt	NX_Q96MI6.
PharmGKB	PA142671151.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000251606.
HOVERGEN	HBG105802.
InParanoid	Q96MI6.
KO	K01090.
PhylomeDB	Q96MI6.
Gene expression databases
ArrayExpress	Q96MI6.
Bgee	Q96MI6.
CleanEx	HS_PPM1E. HS_PPM1M.
Genevestigator	Q96MI6.
GermOnline	ENSG00000164088. Homo sapiens.
Family and domain databases
Gene3D	3.60.40.10. 2 hits.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 2 hits. [Graphical view]
SMART	SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	132160.
NextBio	83025.
SOURCE	Search...

Entry information

Entry name

PPM1M_HUMAN

Accession

Primary (citable) accession number: Q96MI6
Secondary accession number(s): Q8N8J9, Q96DB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.