Q96MG7 (MAGG1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Melanoma-associated antigen G1

Alternative name(s):
Hepatocellular carcinoma-associated protein 4
MAGE-G1 antigen
Necdin-like protein 2

Gene names

Name:	NDNL2
Synonyms:	HCA4, MAGEG1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Growth suppressor that facilitates the entry of the cell into cell cycle arrest By similarity.
Subunit structure	Binds the C-terminus of E2F1 and NGFR By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Tissue specificity	Ubiquitous. Ref.6
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.7 Ref.8 Ref.9
Sequence similarities	Contains 1 MAGE domain.
Sequence caution	The sequence AAH41166.2 differs from that shown. Reason: Erroneous initiation. The sequence BAB84964.1 differs from that shown. Reason: Frameshift at position 290.

Ontologies

Keywords
Biological process	Growth regulation
Cellular component	Cytoplasm Nucleus
Molecular function	Tumor antigen
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 304

304

Melanoma-associated antigen G1

PRO_0000156733

Regions

Domain

85 – 285

201

MAGE

Amino acid modifications

Modified residue

57

1

Phosphoserine Ref.7 Ref.8

Modified residue

60

1

Phosphoserine Ref.7

Modified residue

64

1

Phosphoserine Ref.8 Ref.9

Modified residue

304

1

Phosphoserine Ref.9

Secondary structure

1

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304

Helix Strand Turn

Details...

Helix

82 – 102

21

Helix

108 – 114

7

Helix

119 – 122

4

Helix

123 – 138

16

Beta strand

141 – 146

6

Beta strand

150 – 155

6

Helix

179 – 182

4

Turn

186 – 188

3

Helix

195 – 204

10

Helix

220 – 230

11

Helix

254 – 256

3

Helix

260 – 271

12

Beta strand

275 – 277

3

Helix

278 – 293

16

Sequences

	Sequence		Length	Mass (Da)
	Q96MG7 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: C00E2FB2D2CCED7B	FASTA	304	34,308
	10 20 30 40 50 60 MLQKPRNRGR SGGQAERDRD WSHSGNPGAS RAGEDARVLR DGFAEEAPST SRGPGGSQGS 70 80 90 100 110 120 QGPSPQGARR AQAAPAVGPR SQKQLELKVS ELVQFLLIKD QKKIPIKRAD ILKHVIGDYK 130 140 150 160 170 180 DIFPDLFKRA AERLQYVFGY KLVELEPKSN TYILINTLEP VEEDAEMRGD QGTPTTGLLM 190 200 210 220 230 240 IVLGLIFMKG NTIKETEAWD FLRRLGVYPT KKHLIFGDPK KLITEDFVRQ RYLEYRRIPH 250 260 270 280 290 300 TDPVDYEFQW GPRTNLETSK MKVLKFVAKV HNQDPKDWPA QYCEALADEE NRARPQPSGP APSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of genes in the chromosome X that are differentially expressed in hepatocellular carcinoma." Dong X.-Y., Chen W.-F. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skeletal muscle.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Lung, Spleen and Testis.
[4]	"The nucleotide sequence of a long cDNA clone isolated from human spleen." Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 85-304. Tissue: Spleen.
[5]	"Identification of new genes of the MAGE family." Lucas S., Boon T. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 189-288. Tissue: Testis.
[6]	"A necdin/MAGE-like gene in the chromosome 15 autism susceptibility region: expression, imprinting, and mapping of the human and mouse orthologues." Chibuk T.K., Bischof J.M., Wevrick R. BMC Genet. 2:22-22(2001) [PubMed: 11782285] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57 AND SER-60, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57 AND SER-64, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64 AND SER-304, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF490510 mRNA. Translation: AAM08357.1.
AK056957 mRNA. Translation: BAB71325.1.
BC041166 mRNA. Translation: AAH41166.2. Different initiation.
BC053999 mRNA. Translation: AAH53999.1.
AK074138 mRNA. Translation: BAB84964.1. Frameshift.
AF320911 Genomic DNA. Translation: AAG38607.1.

IPI

IPI00217104.

RefSeq

NP_619649.1. NM_138704.3.

UniGene

Hs.728271.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3NW0	X-ray	2.92	B	78-294	[»]

ProteinModelPortal

Q96MG7.

SMR

Q96MG7. Positions 78-294.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96MG7. 1 interaction.

STRING

Q96MG7.

PTM databases

PhosphoSite

Q96MG7.

Polymorphism databases

DMDM

46396494.

Proteomic databases

PRIDE

Q96MG7.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000332303; ENSP00000330694; ENSG00000185115.

GeneID

56160.

KEGG

hsa:56160.

UCSC

uc001zco.1. human.

Organism-specific databases

CTD

56160.

GeneCards

GC15M029560.

H-InvDB

HIX0012059.

HGNC

HGNC:7677. NDNL2.

MIM

608243. gene.

neXtProt

NX_Q96MG7.

PharmGKB

PA31480.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG08818.

GeneTree

ENSGT00560000076930.

HOGENOM

HBG507249.

HOVERGEN

HBG006315.

InParanoid

Q96MG7.

OMA

IKETEVW.

OrthoDB

EOG44J2JT.

PhylomeDB

Q96MG7.

Enzyme and pathway databases

Pathway_Interaction_DB

p75ntrpathway. p75(NTR)-mediated signaling.

Gene expression databases

ArrayExpress

Q96MG7.

Bgee

Q96MG7.

CleanEx

HS_NDNL2.

Genevestigator

Q96MG7.

GermOnline

ENSG00000185115. Homo sapiens.

Family and domain databases

InterPro

IPR002190. MAGE.
[Graphical view]

PANTHER

PTHR11736. MAGE. 1 hit.

Pfam

PF01454. MAGE. 1 hit.
[Graphical view]

PROSITE

PS50838. MAGE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

61786.

PMAP-CutDB

Q96MG7.

SOURCE

Search...

Entry information

Entry name

MAGG1_HUMAN

Accession

Primary (citable) accession number: Q96MG7
Secondary accession number(s): Q8IW16, Q8TEI6, Q9H214

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.