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Q96MG7

- MAGG1_HUMAN

UniProt

Q96MG7 - MAGG1_HUMAN

Protein

Melanoma-associated antigen G1

Gene

NDNL2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). In vitro enhances ubiquitin ligase activity of NSMCE1. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May be a growth suppressor that facilitates the entry of the cell into cell cycle arrest.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-KW
    2. DNA repair Source: UniProtKB-KW
    3. positive regulation of protein ubiquitination Source: UniProtKB
    4. regulation of growth Source: UniProtKB-KW
    5. regulation of transcription, DNA-templated Source: Ensembl

    Keywords - Molecular functioni

    Tumor antigen

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair, Growth regulation, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Melanoma-associated antigen G1
    Alternative name(s):
    Hepatocellular carcinoma-associated protein 4
    MAGE-G1 antigen
    Necdin-like protein 2
    Gene namesi
    Name:NDNL2
    Synonyms:HCA4, MAGEG1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:7677. NDNL2.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus 1 Publication. Chromosometelomere 1 Publication

    GO - Cellular componenti

    1. chromosome, telomeric region Source: UniProtKB-SubCell
    2. cytoplasm Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell
    4. Smc5-Smc6 complex Source: UniProtKB

    Keywords - Cellular componenti

    Chromosome, Cytoplasm, Nucleus, Telomere

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi96 – 972LL → AA: Decreases interaction with NSMCE1, no effect on interaction with NSMCE4A, abolishes in vitro promotion of NSMCE1 ubiquitin ligase activity. 1 Publication
    Mutagenesisi180 – 1801M → A: Abolishes interaction with EID3. 2 Publications
    Mutagenesisi181 – 1811I → A: Abolishes interaction with EID3. 2 Publications
    Mutagenesisi185 – 1851L → A: Abolishes interaction with EID3. 2 Publications
    Mutagenesisi266 – 2661F → A: Abolishes interaction with EID3. 2 Publications
    Mutagenesisi270 – 2701V → A: Abolishes interaction with EID3. 2 Publications

    Organism-specific databases

    PharmGKBiPA31480.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 304304Melanoma-associated antigen G1PRO_0000156733Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei57 – 571Phosphoserine1 Publication
    Modified residuei64 – 641Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ96MG7.
    PaxDbiQ96MG7.
    PRIDEiQ96MG7.

    PTM databases

    PhosphoSiteiQ96MG7.

    Miscellaneous databases

    PMAP-CutDBQ96MG7.

    Expressioni

    Tissue specificityi

    Ubiquitous.1 Publication

    Gene expression databases

    BgeeiQ96MG7.
    CleanExiHS_NDNL2.
    GenevestigatoriQ96MG7.

    Interactioni

    Subunit structurei

    Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NDNL2. NSMCE1, NSMCE4A or EID3 and NDNL2 probably form a subcomplex that bridges the head domains of the SMC5:SMC6 heterodimer. Interacts with PJA1. Binds the C-terminus of E2F1 and NGFR By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    EID3Q8N1404EBI-2557356,EBI-744483
    NSMCE1Q8WV2215EBI-2557356,EBI-2557372
    NSMCE4AQ9NXX63EBI-2557356,EBI-2557393
    PJA1Q8NG272EBI-2557356,EBI-714606

    Protein-protein interaction databases

    BioGridi121094. 11 interactions.
    IntActiQ96MG7. 8 interactions.
    MINTiMINT-2814584.
    STRINGi9606.ENSP00000330694.

    Structurei

    Secondary structure

    1
    304
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi82 – 10221
    Helixi108 – 1147
    Helixi117 – 1226
    Helixi123 – 13816
    Beta strandi140 – 1467
    Beta strandi150 – 1567
    Helixi174 – 18815
    Turni189 – 1913
    Helixi195 – 20410
    Helixi220 – 23011
    Helixi252 – 2554
    Helixi260 – 27112
    Beta strandi275 – 2773
    Helixi278 – 29316

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3NW0X-ray2.92B78-294[»]
    ProteinModelPortaliQ96MG7.
    SMRiQ96MG7. Positions 78-294.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ96MG7.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini85 – 285201MAGEPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni78 – 304227Interaction with NSMCE1Add
    BLAST

    Sequence similaritiesi

    Contains 1 MAGE domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG292115.
    HOGENOMiHOG000231161.
    HOVERGENiHBG006315.
    InParanoidiQ96MG7.
    OMAiYPTKKHL.
    OrthoDBiEOG75F4GM.
    PhylomeDBiQ96MG7.
    TreeFamiTF328505.

    Family and domain databases

    InterProiIPR002190. MAGE.
    [Graphical view]
    PANTHERiPTHR11736. PTHR11736. 1 hit.
    PfamiPF01454. MAGE. 1 hit.
    [Graphical view]
    PROSITEiPS50838. MAGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q96MG7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLQKPRNRGR SGGQAERDRD WSHSGNPGAS RAGEDARVLR DGFAEEAPST    50
    SRGPGGSQGS QGPSPQGARR AQAAPAVGPR SQKQLELKVS ELVQFLLIKD 100
    QKKIPIKRAD ILKHVIGDYK DIFPDLFKRA AERLQYVFGY KLVELEPKSN 150
    TYILINTLEP VEEDAEMRGD QGTPTTGLLM IVLGLIFMKG NTIKETEAWD 200
    FLRRLGVYPT KKHLIFGDPK KLITEDFVRQ RYLEYRRIPH TDPVDYEFQW 250
    GPRTNLETSK MKVLKFVAKV HNQDPKDWPA QYCEALADEE NRARPQPSGP 300
    APSS 304
    Length:304
    Mass (Da):34,308
    Last modified:December 1, 2001 - v1
    Checksum:iC00E2FB2D2CCED7B
    GO

    Sequence cautioni

    The sequence BAB84964.1 differs from that shown. Reason: Frameshift at position 290.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF490510 mRNA. Translation: AAM08357.1.
    AK056957 mRNA. Translation: BAB71325.1.
    BC041166 mRNA. Translation: AAH41166.2.
    BC053999 mRNA. Translation: AAH53999.1.
    AK074138 mRNA. Translation: BAB84964.1. Frameshift.
    AF320911 Genomic DNA. Translation: AAG38607.1.
    CCDSiCCDS10023.1.
    RefSeqiNP_619649.1. NM_138704.3.
    UniGeneiHs.94011.

    Genome annotation databases

    EnsembliENST00000332303; ENSP00000330694; ENSG00000185115.
    GeneIDi56160.
    KEGGihsa:56160.
    UCSCiuc001zco.3. human.

    Polymorphism databases

    DMDMi46396494.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF490510 mRNA. Translation: AAM08357.1 .
    AK056957 mRNA. Translation: BAB71325.1 .
    BC041166 mRNA. Translation: AAH41166.2 .
    BC053999 mRNA. Translation: AAH53999.1 .
    AK074138 mRNA. Translation: BAB84964.1 . Frameshift.
    AF320911 Genomic DNA. Translation: AAG38607.1 .
    CCDSi CCDS10023.1.
    RefSeqi NP_619649.1. NM_138704.3.
    UniGenei Hs.94011.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3NW0 X-ray 2.92 B 78-294 [» ]
    ProteinModelPortali Q96MG7.
    SMRi Q96MG7. Positions 78-294.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 121094. 11 interactions.
    IntActi Q96MG7. 8 interactions.
    MINTi MINT-2814584.
    STRINGi 9606.ENSP00000330694.

    PTM databases

    PhosphoSitei Q96MG7.

    Polymorphism databases

    DMDMi 46396494.

    Proteomic databases

    MaxQBi Q96MG7.
    PaxDbi Q96MG7.
    PRIDEi Q96MG7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000332303 ; ENSP00000330694 ; ENSG00000185115 .
    GeneIDi 56160.
    KEGGi hsa:56160.
    UCSCi uc001zco.3. human.

    Organism-specific databases

    CTDi 56160.
    GeneCardsi GC15M029560.
    HGNCi HGNC:7677. NDNL2.
    MIMi 608243. gene.
    neXtProti NX_Q96MG7.
    PharmGKBi PA31480.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG292115.
    HOGENOMi HOG000231161.
    HOVERGENi HBG006315.
    InParanoidi Q96MG7.
    OMAi YPTKKHL.
    OrthoDBi EOG75F4GM.
    PhylomeDBi Q96MG7.
    TreeFami TF328505.

    Miscellaneous databases

    EvolutionaryTracei Q96MG7.
    GenomeRNAii 56160.
    NextBioi 61786.
    PMAP-CutDB Q96MG7.
    PROi Q96MG7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q96MG7.
    CleanExi HS_NDNL2.
    Genevestigatori Q96MG7.

    Family and domain databases

    InterProi IPR002190. MAGE.
    [Graphical view ]
    PANTHERi PTHR11736. PTHR11736. 1 hit.
    Pfami PF01454. MAGE. 1 hit.
    [Graphical view ]
    PROSITEi PS50838. MAGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of genes in the chromosome X that are differentially expressed in hepatocellular carcinoma."
      Dong X.-Y., Chen W.-F.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Skeletal muscle.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain, Lung, Spleen and Testis.
    4. "The nucleotide sequence of a long cDNA clone isolated from human spleen."
      Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
      Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 85-304.
      Tissue: Spleen.
    5. "Identification of new genes of the MAGE family."
      Lucas S., Boon T.
      Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 189-288.
      Tissue: Testis.
    6. "A necdin/MAGE-like gene in the chromosome 15 autism susceptibility region: expression, imprinting, and mapping of the human and mouse orthologues."
      Chibuk T.K., Bischof J.M., Wevrick R.
      BMC Genet. 2:22-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    7. "Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex."
      Taylor E.M., Copsey A.C., Hudson J.J., Vidot S., Lehmann A.R.
      Mol. Cell. Biol. 28:1197-1206(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NSMCE1 AND SMC6, IDENTIFICATION IN THE SMC5-SMC6 COMPLEX.
    8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57 AND SER-64, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Interactions between the Nse3 and Nse4 components of the SMC5-6 complex identify evolutionarily conserved interactions between MAGE and EID Families."
      Hudson J.J., Bednarova K., Kozakova L., Liao C., Guerineau M., Colnaghi R., Vidot S., Marek J., Bathula S.R., Lehmann A.R., Palecek J.
      PLoS ONE 6:E17270-E17270(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EID3 AND NSMCE1, MUTAGENESIS OF MET-180; ILE-181; LEU-185; PHE-266 AND VAL-270.
    12. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases."
      Doyle J.M., Gao J., Wang J., Yang M., Potts P.R.
      Mol. Cell 39:963-974(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.92 ANGSTROMS) OF 78-294 IN COMPLEX WITH NSMCE1, FUNCTION, INTERACTION WITH NSMCE1; NSMCE4A AND PJA1, MUTAGENESIS OF 96-LEU-LEU-97.

    Entry informationi

    Entry nameiMAGG1_HUMAN
    AccessioniPrimary (citable) accession number: Q96MG7
    Secondary accession number(s): Q8IW16, Q8TEI6, Q9H214
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3