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Protein

Melanoma-associated antigen G1

Gene

NDNL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). In vitro enhances ubiquitin ligase activity of NSMCE1. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May be a growth suppressor that facilitates the entry of the cell into cell cycle arrest.1 Publication

GO - Biological processi

  1. DNA recombination Source: UniProtKB-KW
  2. DNA repair Source: UniProtKB-KW
  3. positive regulation of protein ubiquitination Source: UniProtKB
  4. regulation of growth Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Tumor antigen

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, Growth regulation, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma-associated antigen G1
Alternative name(s):
Hepatocellular carcinoma-associated protein 4
MAGE-G1 antigen
Necdin-like protein 2
Gene namesi
Name:NDNL2
Synonyms:HCA4, MAGEG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:7677. NDNL2.

Subcellular locationi

  1. Cytoplasm By similarity
  2. Nucleus 1 Publication
  3. Chromosometelomere 1 Publication

GO - Cellular componenti

  1. chromosome, telomeric region Source: UniProtKB-SubCell
  2. cytoplasm Source: UniProtKB-SubCell
  3. nucleus Source: UniProtKB-SubCell
  4. Smc5-Smc6 complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus, Telomere

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi96 – 972LL → AA: Decreases interaction with NSMCE1, no effect on interaction with NSMCE4A, abolishes in vitro promotion of NSMCE1 ubiquitin ligase activity. 1 Publication
Mutagenesisi180 – 1801M → A: Abolishes interaction with EID3. 1 Publication
Mutagenesisi181 – 1811I → A: Abolishes interaction with EID3. 1 Publication
Mutagenesisi185 – 1851L → A: Abolishes interaction with EID3. 1 Publication
Mutagenesisi266 – 2661F → A: Abolishes interaction with EID3. 1 Publication
Mutagenesisi270 – 2701V → A: Abolishes interaction with EID3. 1 Publication

Organism-specific databases

PharmGKBiPA31480.

Polymorphism and mutation databases

BioMutaiNDNL2.
DMDMi46396494.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Melanoma-associated antigen G1PRO_0000156733Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei57 – 571Phosphoserine1 Publication
Modified residuei64 – 641Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ96MG7.
PaxDbiQ96MG7.
PRIDEiQ96MG7.

PTM databases

PhosphoSiteiQ96MG7.

Miscellaneous databases

PMAP-CutDBQ96MG7.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiQ96MG7.
CleanExiHS_NDNL2.
GenevestigatoriQ96MG7.

Interactioni

Subunit structurei

Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NDNL2. NSMCE1, NSMCE4A or EID3 and NDNL2 probably form a subcomplex that bridges the head domains of the SMC5:SMC6 heterodimer. Interacts with PJA1. Binds the C-terminus of E2F1 and NGFR (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
EID3Q8N1404EBI-2557356,EBI-744483
NSMCE1Q8WV2215EBI-2557356,EBI-2557372
NSMCE4AQ9NXX63EBI-2557356,EBI-2557393
PJA1Q8NG272EBI-2557356,EBI-714606

Protein-protein interaction databases

BioGridi121094. 12 interactions.
IntActiQ96MG7. 8 interactions.
MINTiMINT-2814584.
STRINGi9606.ENSP00000330694.

Structurei

Secondary structure

1
304
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi82 – 10221Combined sources
Helixi108 – 1147Combined sources
Helixi117 – 1226Combined sources
Helixi123 – 13816Combined sources
Beta strandi140 – 1467Combined sources
Beta strandi150 – 1567Combined sources
Helixi174 – 18815Combined sources
Turni189 – 1913Combined sources
Helixi195 – 20410Combined sources
Helixi220 – 23011Combined sources
Helixi252 – 2554Combined sources
Helixi260 – 27112Combined sources
Beta strandi275 – 2773Combined sources
Helixi278 – 29316Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3NW0X-ray2.92B78-294[»]
ProteinModelPortaliQ96MG7.
SMRiQ96MG7. Positions 78-294.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96MG7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini85 – 285201MAGEPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni78 – 304227Interaction with NSMCE1Add
BLAST

Sequence similaritiesi

Contains 1 MAGE domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG292115.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000231161.
HOVERGENiHBG006315.
InParanoidiQ96MG7.
OMAiYPTKKHL.
OrthoDBiEOG75F4GM.
PhylomeDBiQ96MG7.
TreeFamiTF328505.

Family and domain databases

InterProiIPR002190. MHD_dom.
[Graphical view]
PANTHERiPTHR11736. PTHR11736. 1 hit.
PfamiPF01454. MAGE. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96MG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQKPRNRGR SGGQAERDRD WSHSGNPGAS RAGEDARVLR DGFAEEAPST
60 70 80 90 100
SRGPGGSQGS QGPSPQGARR AQAAPAVGPR SQKQLELKVS ELVQFLLIKD
110 120 130 140 150
QKKIPIKRAD ILKHVIGDYK DIFPDLFKRA AERLQYVFGY KLVELEPKSN
160 170 180 190 200
TYILINTLEP VEEDAEMRGD QGTPTTGLLM IVLGLIFMKG NTIKETEAWD
210 220 230 240 250
FLRRLGVYPT KKHLIFGDPK KLITEDFVRQ RYLEYRRIPH TDPVDYEFQW
260 270 280 290 300
GPRTNLETSK MKVLKFVAKV HNQDPKDWPA QYCEALADEE NRARPQPSGP

APSS
Length:304
Mass (Da):34,308
Last modified:December 1, 2001 - v1
Checksum:iC00E2FB2D2CCED7B
GO

Sequence cautioni

The sequence BAB84964.1 differs from that shown. Reason: Frameshift at position 290. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF490510 mRNA. Translation: AAM08357.1.
AK056957 mRNA. Translation: BAB71325.1.
BC041166 mRNA. Translation: AAH41166.2.
BC053999 mRNA. Translation: AAH53999.1.
AK074138 mRNA. Translation: BAB84964.1. Frameshift.
AF320911 Genomic DNA. Translation: AAG38607.1.
CCDSiCCDS10023.1.
RefSeqiNP_619649.1. NM_138704.3.
UniGeneiHs.94011.

Genome annotation databases

EnsembliENST00000332303; ENSP00000330694; ENSG00000185115.
GeneIDi56160.
KEGGihsa:56160.
UCSCiuc001zco.3. human.

Polymorphism and mutation databases

BioMutaiNDNL2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF490510 mRNA. Translation: AAM08357.1.
AK056957 mRNA. Translation: BAB71325.1.
BC041166 mRNA. Translation: AAH41166.2.
BC053999 mRNA. Translation: AAH53999.1.
AK074138 mRNA. Translation: BAB84964.1. Frameshift.
AF320911 Genomic DNA. Translation: AAG38607.1.
CCDSiCCDS10023.1.
RefSeqiNP_619649.1. NM_138704.3.
UniGeneiHs.94011.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3NW0X-ray2.92B78-294[»]
ProteinModelPortaliQ96MG7.
SMRiQ96MG7. Positions 78-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121094. 12 interactions.
IntActiQ96MG7. 8 interactions.
MINTiMINT-2814584.
STRINGi9606.ENSP00000330694.

PTM databases

PhosphoSiteiQ96MG7.

Polymorphism and mutation databases

BioMutaiNDNL2.
DMDMi46396494.

Proteomic databases

MaxQBiQ96MG7.
PaxDbiQ96MG7.
PRIDEiQ96MG7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000332303; ENSP00000330694; ENSG00000185115.
GeneIDi56160.
KEGGihsa:56160.
UCSCiuc001zco.3. human.

Organism-specific databases

CTDi56160.
GeneCardsiGC15M029560.
HGNCiHGNC:7677. NDNL2.
MIMi608243. gene.
neXtProtiNX_Q96MG7.
PharmGKBiPA31480.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG292115.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000231161.
HOVERGENiHBG006315.
InParanoidiQ96MG7.
OMAiYPTKKHL.
OrthoDBiEOG75F4GM.
PhylomeDBiQ96MG7.
TreeFamiTF328505.

Miscellaneous databases

EvolutionaryTraceiQ96MG7.
GenomeRNAii56160.
NextBioi61786.
PMAP-CutDBQ96MG7.
PROiQ96MG7.
SOURCEiSearch...

Gene expression databases

BgeeiQ96MG7.
CleanExiHS_NDNL2.
GenevestigatoriQ96MG7.

Family and domain databases

InterProiIPR002190. MHD_dom.
[Graphical view]
PANTHERiPTHR11736. PTHR11736. 1 hit.
PfamiPF01454. MAGE. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of genes in the chromosome X that are differentially expressed in hepatocellular carcinoma."
    Dong X.-Y., Chen W.-F.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skeletal muscle.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain, Lung, Spleen and Testis.
  4. "The nucleotide sequence of a long cDNA clone isolated from human spleen."
    Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 85-304.
    Tissue: Spleen.
  5. "Identification of new genes of the MAGE family."
    Lucas S., Boon T.
    Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 189-288.
    Tissue: Testis.
  6. "A necdin/MAGE-like gene in the chromosome 15 autism susceptibility region: expression, imprinting, and mapping of the human and mouse orthologues."
    Chibuk T.K., Bischof J.M., Wevrick R.
    BMC Genet. 2:22-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex."
    Taylor E.M., Copsey A.C., Hudson J.J., Vidot S., Lehmann A.R.
    Mol. Cell. Biol. 28:1197-1206(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NSMCE1 AND SMC6, IDENTIFICATION IN THE SMC5-SMC6 COMPLEX.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57 AND SER-64, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Interactions between the Nse3 and Nse4 components of the SMC5-6 complex identify evolutionarily conserved interactions between MAGE and EID Families."
    Hudson J.J., Bednarova K., Kozakova L., Liao C., Guerineau M., Colnaghi R., Vidot S., Marek J., Bathula S.R., Lehmann A.R., Palecek J.
    PLoS ONE 6:E17270-E17270(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EID3 AND NSMCE1, MUTAGENESIS OF MET-180; ILE-181; LEU-185; PHE-266 AND VAL-270.
  12. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  14. "MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases."
    Doyle J.M., Gao J., Wang J., Yang M., Potts P.R.
    Mol. Cell 39:963-974(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.92 ANGSTROMS) OF 78-294 IN COMPLEX WITH NSMCE1, FUNCTION, INTERACTION WITH NSMCE1; NSMCE4A AND PJA1, MUTAGENESIS OF 96-LEU-LEU-97.

Entry informationi

Entry nameiMAGG1_HUMAN
AccessioniPrimary (citable) accession number: Q96MG7
Secondary accession number(s): Q8IW16, Q8TEI6, Q9H214
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: December 1, 2001
Last modified: April 29, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.