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Q96MG7

- MAGG1_HUMAN

UniProt

Q96MG7 - MAGG1_HUMAN

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Protein

Melanoma-associated antigen G1

Gene

NDNL2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). In vitro enhances ubiquitin ligase activity of NSMCE1. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May be a growth suppressor that facilitates the entry of the cell into cell cycle arrest.1 Publication

GO - Biological processi

  1. DNA recombination Source: UniProtKB-KW
  2. DNA repair Source: UniProtKB-KW
  3. positive regulation of protein ubiquitination Source: UniProtKB
  4. regulation of growth Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Tumor antigen

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, Growth regulation, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma-associated antigen G1
Alternative name(s):
Hepatocellular carcinoma-associated protein 4
MAGE-G1 antigen
Necdin-like protein 2
Gene namesi
Name:NDNL2
Synonyms:HCA4, MAGEG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:7677. NDNL2.

Subcellular locationi

Cytoplasm By similarity. Nucleus 1 Publication. Chromosometelomere 1 Publication

GO - Cellular componenti

  1. chromosome, telomeric region Source: UniProtKB-KW
  2. cytoplasm Source: UniProtKB-KW
  3. nucleus Source: UniProtKB-KW
  4. Smc5-Smc6 complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus, Telomere

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi96 – 972LL → AA: Decreases interaction with NSMCE1, no effect on interaction with NSMCE4A, abolishes in vitro promotion of NSMCE1 ubiquitin ligase activity. 1 Publication
Mutagenesisi180 – 1801M → A: Abolishes interaction with EID3. 1 Publication
Mutagenesisi181 – 1811I → A: Abolishes interaction with EID3. 1 Publication
Mutagenesisi185 – 1851L → A: Abolishes interaction with EID3. 1 Publication
Mutagenesisi266 – 2661F → A: Abolishes interaction with EID3. 1 Publication
Mutagenesisi270 – 2701V → A: Abolishes interaction with EID3. 1 Publication

Organism-specific databases

PharmGKBiPA31480.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Melanoma-associated antigen G1PRO_0000156733Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei57 – 571Phosphoserine1 Publication
Modified residuei64 – 641Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ96MG7.
PaxDbiQ96MG7.
PRIDEiQ96MG7.

PTM databases

PhosphoSiteiQ96MG7.

Miscellaneous databases

PMAP-CutDBQ96MG7.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiQ96MG7.
CleanExiHS_NDNL2.
GenevestigatoriQ96MG7.

Interactioni

Subunit structurei

Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NDNL2. NSMCE1, NSMCE4A or EID3 and NDNL2 probably form a subcomplex that bridges the head domains of the SMC5:SMC6 heterodimer. Interacts with PJA1. Binds the C-terminus of E2F1 and NGFR (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
EID3Q8N1404EBI-2557356,EBI-744483
NSMCE1Q8WV2215EBI-2557356,EBI-2557372
NSMCE4AQ9NXX63EBI-2557356,EBI-2557393
PJA1Q8NG272EBI-2557356,EBI-714606

Protein-protein interaction databases

BioGridi121094. 13 interactions.
IntActiQ96MG7. 8 interactions.
MINTiMINT-2814584.
STRINGi9606.ENSP00000330694.

Structurei

Secondary structure

1
304
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi82 – 10221Combined sources
Helixi108 – 1147Combined sources
Helixi117 – 1226Combined sources
Helixi123 – 13816Combined sources
Beta strandi140 – 1467Combined sources
Beta strandi150 – 1567Combined sources
Helixi174 – 18815Combined sources
Turni189 – 1913Combined sources
Helixi195 – 20410Combined sources
Helixi220 – 23011Combined sources
Helixi252 – 2554Combined sources
Helixi260 – 27112Combined sources
Beta strandi275 – 2773Combined sources
Helixi278 – 29316Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3NW0X-ray2.92B78-294[»]
ProteinModelPortaliQ96MG7.
SMRiQ96MG7. Positions 78-294.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96MG7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini85 – 285201MAGEPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni78 – 304227Interaction with NSMCE1Add
BLAST

Sequence similaritiesi

Contains 1 MAGE domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG292115.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000231161.
HOVERGENiHBG006315.
InParanoidiQ96MG7.
OMAiYPTKKHL.
OrthoDBiEOG75F4GM.
PhylomeDBiQ96MG7.
TreeFamiTF328505.

Family and domain databases

InterProiIPR002190. MAGE.
[Graphical view]
PANTHERiPTHR11736. PTHR11736. 1 hit.
PfamiPF01454. MAGE. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96MG7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLQKPRNRGR SGGQAERDRD WSHSGNPGAS RAGEDARVLR DGFAEEAPST
60 70 80 90 100
SRGPGGSQGS QGPSPQGARR AQAAPAVGPR SQKQLELKVS ELVQFLLIKD
110 120 130 140 150
QKKIPIKRAD ILKHVIGDYK DIFPDLFKRA AERLQYVFGY KLVELEPKSN
160 170 180 190 200
TYILINTLEP VEEDAEMRGD QGTPTTGLLM IVLGLIFMKG NTIKETEAWD
210 220 230 240 250
FLRRLGVYPT KKHLIFGDPK KLITEDFVRQ RYLEYRRIPH TDPVDYEFQW
260 270 280 290 300
GPRTNLETSK MKVLKFVAKV HNQDPKDWPA QYCEALADEE NRARPQPSGP

APSS
Length:304
Mass (Da):34,308
Last modified:December 1, 2001 - v1
Checksum:iC00E2FB2D2CCED7B
GO

Sequence cautioni

The sequence BAB84964.1 differs from that shown. Reason: Frameshift at position 290. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF490510 mRNA. Translation: AAM08357.1.
AK056957 mRNA. Translation: BAB71325.1.
BC041166 mRNA. Translation: AAH41166.2.
BC053999 mRNA. Translation: AAH53999.1.
AK074138 mRNA. Translation: BAB84964.1. Frameshift.
AF320911 Genomic DNA. Translation: AAG38607.1.
CCDSiCCDS10023.1.
RefSeqiNP_619649.1. NM_138704.3.
UniGeneiHs.94011.

Genome annotation databases

EnsembliENST00000332303; ENSP00000330694; ENSG00000185115.
GeneIDi56160.
KEGGihsa:56160.
UCSCiuc001zco.3. human.

Polymorphism databases

DMDMi46396494.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF490510 mRNA. Translation: AAM08357.1 .
AK056957 mRNA. Translation: BAB71325.1 .
BC041166 mRNA. Translation: AAH41166.2 .
BC053999 mRNA. Translation: AAH53999.1 .
AK074138 mRNA. Translation: BAB84964.1 . Frameshift.
AF320911 Genomic DNA. Translation: AAG38607.1 .
CCDSi CCDS10023.1.
RefSeqi NP_619649.1. NM_138704.3.
UniGenei Hs.94011.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3NW0 X-ray 2.92 B 78-294 [» ]
ProteinModelPortali Q96MG7.
SMRi Q96MG7. Positions 78-294.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121094. 13 interactions.
IntActi Q96MG7. 8 interactions.
MINTi MINT-2814584.
STRINGi 9606.ENSP00000330694.

PTM databases

PhosphoSitei Q96MG7.

Polymorphism databases

DMDMi 46396494.

Proteomic databases

MaxQBi Q96MG7.
PaxDbi Q96MG7.
PRIDEi Q96MG7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000332303 ; ENSP00000330694 ; ENSG00000185115 .
GeneIDi 56160.
KEGGi hsa:56160.
UCSCi uc001zco.3. human.

Organism-specific databases

CTDi 56160.
GeneCardsi GC15M029560.
HGNCi HGNC:7677. NDNL2.
MIMi 608243. gene.
neXtProti NX_Q96MG7.
PharmGKBi PA31480.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG292115.
GeneTreei ENSGT00760000118824.
HOGENOMi HOG000231161.
HOVERGENi HBG006315.
InParanoidi Q96MG7.
OMAi YPTKKHL.
OrthoDBi EOG75F4GM.
PhylomeDBi Q96MG7.
TreeFami TF328505.

Miscellaneous databases

EvolutionaryTracei Q96MG7.
GenomeRNAii 56160.
NextBioi 61786.
PMAP-CutDB Q96MG7.
PROi Q96MG7.
SOURCEi Search...

Gene expression databases

Bgeei Q96MG7.
CleanExi HS_NDNL2.
Genevestigatori Q96MG7.

Family and domain databases

InterProi IPR002190. MAGE.
[Graphical view ]
PANTHERi PTHR11736. PTHR11736. 1 hit.
Pfami PF01454. MAGE. 1 hit.
[Graphical view ]
PROSITEi PS50838. MAGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of genes in the chromosome X that are differentially expressed in hepatocellular carcinoma."
    Dong X.-Y., Chen W.-F.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skeletal muscle.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain, Lung, Spleen and Testis.
  4. "The nucleotide sequence of a long cDNA clone isolated from human spleen."
    Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 85-304.
    Tissue: Spleen.
  5. "Identification of new genes of the MAGE family."
    Lucas S., Boon T.
    Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 189-288.
    Tissue: Testis.
  6. "A necdin/MAGE-like gene in the chromosome 15 autism susceptibility region: expression, imprinting, and mapping of the human and mouse orthologues."
    Chibuk T.K., Bischof J.M., Wevrick R.
    BMC Genet. 2:22-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex."
    Taylor E.M., Copsey A.C., Hudson J.J., Vidot S., Lehmann A.R.
    Mol. Cell. Biol. 28:1197-1206(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NSMCE1 AND SMC6, IDENTIFICATION IN THE SMC5-SMC6 COMPLEX.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57 AND SER-64, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Interactions between the Nse3 and Nse4 components of the SMC5-6 complex identify evolutionarily conserved interactions between MAGE and EID Families."
    Hudson J.J., Bednarova K., Kozakova L., Liao C., Guerineau M., Colnaghi R., Vidot S., Marek J., Bathula S.R., Lehmann A.R., Palecek J.
    PLoS ONE 6:E17270-E17270(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EID3 AND NSMCE1, MUTAGENESIS OF MET-180; ILE-181; LEU-185; PHE-266 AND VAL-270.
  12. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases."
    Doyle J.M., Gao J., Wang J., Yang M., Potts P.R.
    Mol. Cell 39:963-974(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.92 ANGSTROMS) OF 78-294 IN COMPLEX WITH NSMCE1, FUNCTION, INTERACTION WITH NSMCE1; NSMCE4A AND PJA1, MUTAGENESIS OF 96-LEU-LEU-97.

Entry informationi

Entry nameiMAGG1_HUMAN
AccessioniPrimary (citable) accession number: Q96MG7
Secondary accession number(s): Q8IW16, Q8TEI6, Q9H214
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: December 1, 2001
Last modified: November 26, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3