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Q96MG7 (MAGG1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Melanoma-associated antigen G1
Alternative name(s):
Hepatocellular carcinoma-associated protein 4
MAGE-G1 antigen
Necdin-like protein 2
Gene names
Name:NDNL2
Synonyms:HCA4, MAGEG1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). In vitro enhances ubiquitin ligase activity of NSMCE1. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May be a growth suppressor that facilitates the entry of the cell into cell cycle arrest. Ref.13

Subunit structure

Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NDNL2. NSMCE1, NSMCE4A or EID3 and NDNL2 probably form a subcomplex that bridges the head domains of the SMC5:SMC6 heterodimer. Interacts with PJA1. Binds the C-terminus of E2F1 and NGFR By similarity. Ref.7 Ref.11 Ref.13

Subcellular location

Cytoplasm By similarity. Nucleus. Chromosometelomere Probable Ref.7.

Tissue specificity

Ubiquitous. Ref.6

Sequence similarities

Contains 1 MAGE domain.

Sequence caution

The sequence BAB84964.1 differs from that shown. Reason: Frameshift at position 290.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 304304Melanoma-associated antigen G1
PRO_0000156733

Regions

Domain85 – 285201MAGE
Region78 – 304227Interaction with NSMCE1

Amino acid modifications

Modified residue571Phosphoserine Ref.8
Modified residue641Phosphoserine Ref.8

Experimental info

Mutagenesis96 – 972LL → AA: Decreases interaction with NSMCE1, no effect on interaction with NSMCE4A, abolishes in vitro promotion of NSMCE1 ubiquitin ligase activity. Ref.13
Mutagenesis1801M → A: Abolishes interaction with EID3. Ref.11 Ref.13
Mutagenesis1811I → A: Abolishes interaction with EID3. Ref.11 Ref.13
Mutagenesis1851L → A: Abolishes interaction with EID3. Ref.11 Ref.13
Mutagenesis2661F → A: Abolishes interaction with EID3. Ref.11 Ref.13
Mutagenesis2701V → A: Abolishes interaction with EID3. Ref.11 Ref.13

Secondary structure

.......................... 304
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q96MG7 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: C00E2FB2D2CCED7B

FASTA30434,308
        10         20         30         40         50         60 
MLQKPRNRGR SGGQAERDRD WSHSGNPGAS RAGEDARVLR DGFAEEAPST SRGPGGSQGS 

        70         80         90        100        110        120 
QGPSPQGARR AQAAPAVGPR SQKQLELKVS ELVQFLLIKD QKKIPIKRAD ILKHVIGDYK 

       130        140        150        160        170        180 
DIFPDLFKRA AERLQYVFGY KLVELEPKSN TYILINTLEP VEEDAEMRGD QGTPTTGLLM 

       190        200        210        220        230        240 
IVLGLIFMKG NTIKETEAWD FLRRLGVYPT KKHLIFGDPK KLITEDFVRQ RYLEYRRIPH 

       250        260        270        280        290        300 
TDPVDYEFQW GPRTNLETSK MKVLKFVAKV HNQDPKDWPA QYCEALADEE NRARPQPSGP 


APSS 

« Hide

References

« Hide 'large scale' references
[1]"Identification of genes in the chromosome X that are differentially expressed in hepatocellular carcinoma."
Dong X.-Y., Chen W.-F.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skeletal muscle.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain, Lung, Spleen and Testis.
[4]"The nucleotide sequence of a long cDNA clone isolated from human spleen."
Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 85-304.
Tissue: Spleen.
[5]"Identification of new genes of the MAGE family."
Lucas S., Boon T.
Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 189-288.
Tissue: Testis.
[6]"A necdin/MAGE-like gene in the chromosome 15 autism susceptibility region: expression, imprinting, and mapping of the human and mouse orthologues."
Chibuk T.K., Bischof J.M., Wevrick R.
BMC Genet. 2:22-22(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex."
Taylor E.M., Copsey A.C., Hudson J.J., Vidot S., Lehmann A.R.
Mol. Cell. Biol. 28:1197-1206(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NSMCE1 AND SMC6, IDENTIFICATION IN THE SMC5-SMC6 COMPLEX.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57 AND SER-64, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Interactions between the Nse3 and Nse4 components of the SMC5-6 complex identify evolutionarily conserved interactions between MAGE and EID Families."
Hudson J.J., Bednarova K., Kozakova L., Liao C., Guerineau M., Colnaghi R., Vidot S., Marek J., Bathula S.R., Lehmann A.R., Palecek J.
PLoS ONE 6:E17270-E17270(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EID3 AND NSMCE1, MUTAGENESIS OF MET-180; ILE-181; LEU-185; PHE-266 AND VAL-270.
[12]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases."
Doyle J.M., Gao J., Wang J., Yang M., Potts P.R.
Mol. Cell 39:963-974(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.92 ANGSTROMS) OF 78-294 IN COMPLEX WITH NSMCE1, FUNCTION, INTERACTION WITH NSMCE1; NSMCE4A AND PJA1, MUTAGENESIS OF 96-LEU-LEU-97.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF490510 mRNA. Translation: AAM08357.1.
AK056957 mRNA. Translation: BAB71325.1.
BC041166 mRNA. Translation: AAH41166.2.
BC053999 mRNA. Translation: AAH53999.1.
AK074138 mRNA. Translation: BAB84964.1. Frameshift.
AF320911 Genomic DNA. Translation: AAG38607.1.
CCDSCCDS10023.1.
RefSeqNP_619649.1. NM_138704.3.
UniGeneHs.94011.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3NW0X-ray2.92B78-294[»]
ProteinModelPortalQ96MG7.
SMRQ96MG7. Positions 78-294.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121094. 11 interactions.
IntActQ96MG7. 8 interactions.
MINTMINT-2814584.
STRING9606.ENSP00000330694.

PTM databases

PhosphoSiteQ96MG7.

Polymorphism databases

DMDM46396494.

Proteomic databases

MaxQBQ96MG7.
PaxDbQ96MG7.
PRIDEQ96MG7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000332303; ENSP00000330694; ENSG00000185115.
GeneID56160.
KEGGhsa:56160.
UCSCuc001zco.3. human.

Organism-specific databases

CTD56160.
GeneCardsGC15M029560.
HGNCHGNC:7677. NDNL2.
MIM608243. gene.
neXtProtNX_Q96MG7.
PharmGKBPA31480.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG292115.
HOGENOMHOG000231161.
HOVERGENHBG006315.
InParanoidQ96MG7.
OMAYPTKKHL.
OrthoDBEOG75F4GM.
PhylomeDBQ96MG7.
TreeFamTF328505.

Gene expression databases

BgeeQ96MG7.
CleanExHS_NDNL2.
GenevestigatorQ96MG7.

Family and domain databases

InterProIPR002190. MAGE.
[Graphical view]
PANTHERPTHR11736. PTHR11736. 1 hit.
PfamPF01454. MAGE. 1 hit.
[Graphical view]
PROSITEPS50838. MAGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ96MG7.
GenomeRNAi56160.
NextBio61786.
PMAP-CutDBQ96MG7.
PROQ96MG7.
SOURCESearch...

Entry information

Entry nameMAGG1_HUMAN
AccessionPrimary (citable) accession number: Q96MG7
Secondary accession number(s): Q8IW16, Q8TEI6, Q9H214
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM