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Protein

Adenylate kinase 8

Gene

AK8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP. Also displays broad nucleoside diphosphate kinase activity.2 Publications

Catalytic activityi

ATP + AMP = 2 ADP.
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

Kineticsi

  1. KM=4.1 µM for AMP1 Publication
  2. KM=630 µM for dAMP1 Publication
  3. KM=1.4 µM for CMP1 Publication
  1. Vmax=2400 pmol/min/µg enzyme with AMP as substrate1 Publication
  2. Vmax=1360 pmol/min/µg enzyme with dAMP as substrate1 Publication
  3. Vmax=190 pmol/min/µg enzyme with CMP as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei147 – 1471AMP 1By similarity
Binding sitei203 – 2031AMP 1By similarity
Binding sitei361 – 3611AMP 2By similarity
Binding sitei392 – 3921ATP 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi67 – 726ATP 1By similarity
Nucleotide bindingi140 – 1434AMP 1By similarity
Nucleotide bindingi278 – 2836ATP 2By similarity
Nucleotide bindingi325 – 3273AMP 2By similarity
Nucleotide bindingi354 – 3574AMP 2By similarity

GO - Molecular functioni

  • adenylate kinase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • cytidylate kinase activity Source: UniProtKB
  • nucleoside diphosphate kinase activity Source: UniProtKB

GO - Biological processi

  • nucleoside diphosphate phosphorylation Source: UniProtKB
  • nucleoside triphosphate biosynthetic process Source: UniProtKB
  • ventricular system development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase 8 (EC:2.7.4.3, EC:2.7.4.6)
Short name:
AK 8
Alternative name(s):
ATP-AMP transphosphorylase 8
Gene namesi
Name:AK8
Synonyms:C9orf98
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:26526. AK8.

Subcellular locationi

  • Cytoplasmcytosol 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134971772.

Polymorphism and mutation databases

BioMutaiAK8.
DMDMi74752032.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Adenylate kinase 8PRO_0000279383Add
BLAST

Proteomic databases

PaxDbiQ96MA6.
PRIDEiQ96MA6.

PTM databases

PhosphoSiteiQ96MA6.

Expressioni

Gene expression databases

BgeeiQ96MA6.
CleanExiHS_C9orf98.
GenevisibleiQ96MA6. HS.

Organism-specific databases

HPAiHPA021443.
HPA021445.
HPA023894.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ALKBH3Q96Q833EBI-8466265,EBI-6658697
CCDC102BA1A4H13EBI-8466265,EBI-10171570
HAUS1Q96CS23EBI-8466265,EBI-2514791
MAP3K7CLP57077-43EBI-8466265,EBI-10215880
MCM7P339933EBI-8466265,EBI-355924
MID1IP1Q9NPA33EBI-8466265,EBI-750096
RBM39Q144983EBI-8466265,EBI-395290
RBM39Q14498-33EBI-8466265,EBI-6654703
UBE2ZQ9H8323EBI-8466265,EBI-720977
ZMAT2Q96NC03EBI-8466265,EBI-2682299

Protein-protein interaction databases

BioGridi127645. 10 interactions.
IntActiQ96MA6. 10 interactions.
MINTiMINT-8417639.
STRINGi9606.ENSP00000298545.

Structurei

3D structure databases

ProteinModelPortaliQ96MA6.
SMRiQ96MA6. Positions 59-250, 270-477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni58 – 258201Adenylate kinase 1Add
BLAST
Regioni87 – 11327NMPbind 1By similarityAdd
BLAST
Regioni177 – 20630LID 1By similarityAdd
BLAST
Regioni269 – 471203Adenylate kinase 2Add
BLAST
Regioni298 – 32730NMPbind 2By similarityAdd
BLAST
Regioni391 – 42434LID 2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the adenylate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0563.
GeneTreeiENSGT00550000074679.
HOVERGENiHBG080569.
InParanoidiQ96MA6.
KOiK00939.
OMAiEYHRNIV.
OrthoDBiEOG71CFKV.
PhylomeDBiQ96MA6.
TreeFamiTF328560.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00235. Adenylate_kinase_Adk.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 3 hits.
SSF57774. SSF57774. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96MA6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDATIAPHRI PPEMPQYGEE NHIFELMQNM LEQLLIHQPE DPIPFMIQHL
60 70 80 90 100
HRDNDNVPRI VILGPPASGK TTIAMWLCKH LNSSLLTLEN LILNEFSYTA
110 120 130 140 150
TEARRLYLQR KTVPSALLVQ LIQERLAEED CIKQGWILDG IPETREQALR
160 170 180 190 200
IQTLGITPRH VIVLSAPDTV LIERNLGKRI DPQTGEIYHT TFDWPPESEI
210 220 230 240 250
QNRLMVPEDI SELETAQKLL EYHRNIVRVI PSYPKILKVI SADQPCVDVF
260 270 280 290 300
YQALTYVQSN HRTNAPFTPR VLLLGPVGSG KSLQAALLAQ KYRLVNVCCG
310 320 330 340 350
QLLKEAVADR TTFGELIQPF FEKEMAVPDS LLMKVLSQRL DQQDCIQKGW
360 370 380 390 400
VLHGVPRDLD QAHLLNRLGY NPNRVFFLNV PFDSIMERLT LRRIDPVTGE
410 420 430 440 450
RYHLMYKPPP TMEIQARLLQ NPKDAEEQVK LKMDLFYRNS ADLEQLYGSA
460 470
ITLNGDQDPY TVFEYIESGI INPLPKKIP
Length:479
Mass (Da):54,926
Last modified:December 1, 2001 - v1
Checksum:i9E4EABA3F429B731
GO
Isoform 2 (identifier: Q96MA6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-204: Missing.

Note: No experimental confirmation available.
Show »
Length:275
Mass (Da):31,455
Checksum:iB90C76CF137D6C34
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti5 – 51I → T.
Corresponds to variant rs2231400 [ dbSNP | Ensembl ].
VAR_030873
Natural varianti130 – 1301D → G.
Corresponds to variant rs17407084 [ dbSNP | Ensembl ].
VAR_030874

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 204204Missing in isoform 2. 1 PublicationVSP_023419Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057266 mRNA. Translation: BAB71402.1.
AK093446 mRNA. Translation: BAC04168.1.
AK292186 mRNA. Translation: BAF84875.1.
AL160165, AL445645 Genomic DNA. Translation: CAH70535.1.
AL445645, AL160165 Genomic DNA. Translation: CAH72109.1.
CH471090 Genomic DNA. Translation: EAW88016.1.
BC034776 mRNA. Translation: AAH34776.1.
BC050576 mRNA. Translation: AAH50576.1.
CCDSiCCDS6954.1. [Q96MA6-1]
RefSeqiNP_689785.1. NM_152572.2. [Q96MA6-1]
UniGeneiHs.421340.

Genome annotation databases

EnsembliENST00000298545; ENSP00000298545; ENSG00000165695. [Q96MA6-1]
GeneIDi158067.
KEGGihsa:158067.
UCSCiuc004cbu.1. human. [Q96MA6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057266 mRNA. Translation: BAB71402.1.
AK093446 mRNA. Translation: BAC04168.1.
AK292186 mRNA. Translation: BAF84875.1.
AL160165, AL445645 Genomic DNA. Translation: CAH70535.1.
AL445645, AL160165 Genomic DNA. Translation: CAH72109.1.
CH471090 Genomic DNA. Translation: EAW88016.1.
BC034776 mRNA. Translation: AAH34776.1.
BC050576 mRNA. Translation: AAH50576.1.
CCDSiCCDS6954.1. [Q96MA6-1]
RefSeqiNP_689785.1. NM_152572.2. [Q96MA6-1]
UniGeneiHs.421340.

3D structure databases

ProteinModelPortaliQ96MA6.
SMRiQ96MA6. Positions 59-250, 270-477.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127645. 10 interactions.
IntActiQ96MA6. 10 interactions.
MINTiMINT-8417639.
STRINGi9606.ENSP00000298545.

PTM databases

PhosphoSiteiQ96MA6.

Polymorphism and mutation databases

BioMutaiAK8.
DMDMi74752032.

Proteomic databases

PaxDbiQ96MA6.
PRIDEiQ96MA6.

Protocols and materials databases

DNASUi158067.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000298545; ENSP00000298545; ENSG00000165695. [Q96MA6-1]
GeneIDi158067.
KEGGihsa:158067.
UCSCiuc004cbu.1. human. [Q96MA6-1]

Organism-specific databases

CTDi158067.
GeneCardsiGC09M135601.
H-InvDBHIX0008494.
HGNCiHGNC:26526. AK8.
HPAiHPA021443.
HPA021445.
HPA023894.
MIMi615365. gene.
neXtProtiNX_Q96MA6.
PharmGKBiPA134971772.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0563.
GeneTreeiENSGT00550000074679.
HOVERGENiHBG080569.
InParanoidiQ96MA6.
KOiK00939.
OMAiEYHRNIV.
OrthoDBiEOG71CFKV.
PhylomeDBiQ96MA6.
TreeFamiTF328560.

Miscellaneous databases

ChiTaRSiAK8. human.
GenomeRNAii158067.
NextBioi87622.
PROiQ96MA6.
SOURCEiSearch...

Gene expression databases

BgeeiQ96MA6.
CleanExiHS_C9orf98.
GenevisibleiQ96MA6. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00235. Adenylate_kinase_Adk.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 3 hits.
SSF57774. SSF57774. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Testis.
  2. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  5. "The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes."
    Panayiotou C., Solaroli N., Xu Y., Johansson M., Karlsson A.
    Biochem. J. 433:527-534(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY.
  6. "The human adenylate kinase 9 is a nucleoside mono- and diphosphate kinase."
    Amiri M., Conserva F., Panayiotou C., Karlsson A., Solaroli N.
    Int. J. Biochem. Cell Biol. 45:925-931(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiKAD8_HUMAN
AccessioniPrimary (citable) accession number: Q96MA6
Secondary accession number(s): A8K821, Q8N9W9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: December 1, 2001
Last modified: June 24, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.