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Protein

Adenylate kinase 8

Gene

AK8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP. Also displays broad nucleoside diphosphate kinase activity.2 Publications

Catalytic activityi

ATP + AMP = 2 ADP.1 Publication
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.2 Publications

Kineticsi

  1. KM=4.1 µM for AMP1 Publication
  2. KM=630 µM for dAMP1 Publication
  3. KM=1.4 µM for CMP1 Publication
  1. Vmax=2400 pmol/min/µg enzyme with AMP as substrate1 Publication
  2. Vmax=1360 pmol/min/µg enzyme with dAMP as substrate1 Publication
  3. Vmax=190 pmol/min/µg enzyme with CMP as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei147AMP 1By similarity1
Binding sitei203AMP 1By similarity1
Binding sitei361AMP 2By similarity1
Binding sitei392ATP 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi67 – 72ATP 1By similarity6
Nucleotide bindingi140 – 143AMP 1By similarity4
Nucleotide bindingi278 – 283ATP 2By similarity6
Nucleotide bindingi325 – 327AMP 2By similarity3
Nucleotide bindingi354 – 357AMP 2By similarity4

GO - Molecular functioni

  • adenylate kinase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • cytidylate kinase activity Source: UniProtKB
  • nucleoside diphosphate kinase activity Source: UniProtKB
  • nucleoside kinase activity Source: Reactome

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-499943. Interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase 8 (EC:2.7.4.31 Publication, EC:2.7.4.62 Publications)
Short name:
AK 8
Alternative name(s):
ATP-AMP transphosphorylase 8
Gene namesi
Name:AK8
Synonyms:C9orf98
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000165695.9.
HGNCiHGNC:26526. AK8.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi158067.
OpenTargetsiENSG00000165695.
PharmGKBiPA134971772.

Chemistry databases

DrugBankiDB01717. Bis(Adenosine)-5'-Pentaphosphate.

Polymorphism and mutation databases

BioMutaiAK8.
DMDMi74752032.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002793831 – 479Adenylate kinase 8Add BLAST479

Proteomic databases

PaxDbiQ96MA6.
PeptideAtlasiQ96MA6.
PRIDEiQ96MA6.

PTM databases

iPTMnetiQ96MA6.
PhosphoSitePlusiQ96MA6.

Expressioni

Gene expression databases

BgeeiENSG00000165695.
CleanExiHS_C9orf98.
GenevisibleiQ96MA6. HS.

Organism-specific databases

HPAiHPA021443.
HPA021445.
HPA023894.

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi127645. 10 interactors.
IntActiQ96MA6. 15 interactors.
MINTiMINT-8417639.
STRINGi9606.ENSP00000298545.

Structurei

3D structure databases

ProteinModelPortaliQ96MA6.
SMRiQ96MA6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 258Adenylate kinase 11 PublicationAdd BLAST201
Regioni87 – 113NMPbind 1By similarityAdd BLAST27
Regioni177 – 206LID 1By similarityAdd BLAST30
Regioni269 – 471Adenylate kinase 21 PublicationAdd BLAST203
Regioni298 – 327NMPbind 2By similarityAdd BLAST30
Regioni391 – 424LID 2By similarityAdd BLAST34

Sequence similaritiesi

Belongs to the adenylate kinase family.Curated

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00830000128340.
HOVERGENiHBG080569.
InParanoidiQ96MA6.
KOiK00939.
OMAiERYHLMY.
OrthoDBiEOG091G06BH.
PhylomeDBiQ96MA6.
TreeFamiTF328560.

Family and domain databases

CDDicd01428. ADK. 2 hits.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
InterProiView protein in InterPro
IPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR027417. P-loop_NTPase.
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 3 hits.
SSF57774. SSF57774. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96MA6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDATIAPHRI PPEMPQYGEE NHIFELMQNM LEQLLIHQPE DPIPFMIQHL
60 70 80 90 100
HRDNDNVPRI VILGPPASGK TTIAMWLCKH LNSSLLTLEN LILNEFSYTA
110 120 130 140 150
TEARRLYLQR KTVPSALLVQ LIQERLAEED CIKQGWILDG IPETREQALR
160 170 180 190 200
IQTLGITPRH VIVLSAPDTV LIERNLGKRI DPQTGEIYHT TFDWPPESEI
210 220 230 240 250
QNRLMVPEDI SELETAQKLL EYHRNIVRVI PSYPKILKVI SADQPCVDVF
260 270 280 290 300
YQALTYVQSN HRTNAPFTPR VLLLGPVGSG KSLQAALLAQ KYRLVNVCCG
310 320 330 340 350
QLLKEAVADR TTFGELIQPF FEKEMAVPDS LLMKVLSQRL DQQDCIQKGW
360 370 380 390 400
VLHGVPRDLD QAHLLNRLGY NPNRVFFLNV PFDSIMERLT LRRIDPVTGE
410 420 430 440 450
RYHLMYKPPP TMEIQARLLQ NPKDAEEQVK LKMDLFYRNS ADLEQLYGSA
460 470
ITLNGDQDPY TVFEYIESGI INPLPKKIP
Length:479
Mass (Da):54,926
Last modified:December 1, 2001 - v1
Checksum:i9E4EABA3F429B731
GO
Isoform 2 (identifier: Q96MA6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-204: Missing.

Note: No experimental confirmation available.
Show »
Length:275
Mass (Da):31,455
Checksum:iB90C76CF137D6C34
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0308735I → T. Corresponds to variant dbSNP:rs2231400Ensembl.1
Natural variantiVAR_030874130D → G. Corresponds to variant dbSNP:rs17407084Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0234191 – 204Missing in isoform 2. 1 PublicationAdd BLAST204

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057266 mRNA. Translation: BAB71402.1.
AK093446 mRNA. Translation: BAC04168.1.
AK292186 mRNA. Translation: BAF84875.1.
AL160165, AL445645 Genomic DNA. Translation: CAH70535.1.
AL445645, AL160165 Genomic DNA. Translation: CAH72109.1.
CH471090 Genomic DNA. Translation: EAW88016.1.
BC034776 mRNA. Translation: AAH34776.1.
BC050576 mRNA. Translation: AAH50576.1.
CCDSiCCDS6954.1. [Q96MA6-1]
RefSeqiNP_001304887.1. NM_001317958.1. [Q96MA6-2]
NP_001304888.1. NM_001317959.1.
NP_689785.1. NM_152572.2. [Q96MA6-1]
UniGeneiHs.421340.

Genome annotation databases

EnsembliENST00000298545; ENSP00000298545; ENSG00000165695. [Q96MA6-1]
GeneIDi158067.
KEGGihsa:158067.
UCSCiuc004cbu.2. human. [Q96MA6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiKAD8_HUMAN
AccessioniPrimary (citable) accession number: Q96MA6
Secondary accession number(s): A8K821, Q8N9W9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: December 1, 2001
Last modified: September 27, 2017
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families