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Q96MA6 (KAD8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenylate kinase 8

Short name=AK 8
EC=2.7.4.3
EC=2.7.4.6
Alternative name(s):
ATP-AMP transphosphorylase 8
Gene names
Name:AK8
Synonyms:C9orf98
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP. Also displays broad nucleoside diphosphate kinase activity. Ref.5 Ref.6

Catalytic activity

ATP + AMP = 2 ADP. Ref.5 Ref.6

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate. Ref.5 Ref.6

Subcellular location

Cytoplasmcytosol Ref.5.

Sequence similarities

Belongs to the adenylate kinase family.

Biophysicochemical properties

Kinetic parameters:

KM=4.1 µM for AMP Ref.5

KM=630 µM for dAMP

KM=1.4 µM for CMP

Vmax=2400 pmol/min/µg enzyme with AMP as substrate

Vmax=1360 pmol/min/µg enzyme with dAMP as substrate

Vmax=190 pmol/min/µg enzyme with CMP as substrate

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96MA6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96MA6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-204: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 479479Adenylate kinase 8 HAMAP-Rule MF_00235
PRO_0000279383

Regions

Nucleotide binding67 – 726ATP 1 By similarity
Nucleotide binding140 – 1434AMP 1 By similarity
Nucleotide binding278 – 2836ATP 2 By similarity
Nucleotide binding325 – 3273AMP 2 By similarity
Nucleotide binding354 – 3574AMP 2 By similarity
Region58 – 258201Adenylate kinase 1 HAMAP-Rule MF_00235
Region87 – 11327NMPbind 1 By similarity
Region177 – 20630LID 1 By similarity
Region269 – 471203Adenylate kinase 2 HAMAP-Rule MF_00235
Region298 – 32730NMPbind 2 By similarity
Region391 – 42434LID 2 By similarity

Sites

Binding site1471AMP 1 By similarity
Binding site2031AMP 1 By similarity
Binding site3611AMP 2 By similarity
Binding site3921ATP 2 By similarity

Natural variations

Alternative sequence1 – 204204Missing in isoform 2.
VSP_023419
Natural variant51I → T.
Corresponds to variant rs2231400 [ dbSNP | Ensembl ].
VAR_030873
Natural variant1301D → G.
Corresponds to variant rs17407084 [ dbSNP | Ensembl ].
VAR_030874

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 9E4EABA3F429B731

FASTA47954,926
        10         20         30         40         50         60 
MDATIAPHRI PPEMPQYGEE NHIFELMQNM LEQLLIHQPE DPIPFMIQHL HRDNDNVPRI 

        70         80         90        100        110        120 
VILGPPASGK TTIAMWLCKH LNSSLLTLEN LILNEFSYTA TEARRLYLQR KTVPSALLVQ 

       130        140        150        160        170        180 
LIQERLAEED CIKQGWILDG IPETREQALR IQTLGITPRH VIVLSAPDTV LIERNLGKRI 

       190        200        210        220        230        240 
DPQTGEIYHT TFDWPPESEI QNRLMVPEDI SELETAQKLL EYHRNIVRVI PSYPKILKVI 

       250        260        270        280        290        300 
SADQPCVDVF YQALTYVQSN HRTNAPFTPR VLLLGPVGSG KSLQAALLAQ KYRLVNVCCG 

       310        320        330        340        350        360 
QLLKEAVADR TTFGELIQPF FEKEMAVPDS LLMKVLSQRL DQQDCIQKGW VLHGVPRDLD 

       370        380        390        400        410        420 
QAHLLNRLGY NPNRVFFLNV PFDSIMERLT LRRIDPVTGE RYHLMYKPPP TMEIQARLLQ 

       430        440        450        460        470 
NPKDAEEQVK LKMDLFYRNS ADLEQLYGSA ITLNGDQDPY TVFEYIESGI INPLPKKIP 

« Hide

Isoform 2 [UniParc].

Checksum: B90C76CF137D6C34
Show »

FASTA27531,455

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Testis.
[2]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[5]"The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes."
Panayiotou C., Solaroli N., Xu Y., Johansson M., Karlsson A.
Biochem. J. 433:527-534(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY.
[6]"The human adenylate kinase 9 is a nucleoside mono- and diphosphate kinase."
Amiri M., Conserva F., Panayiotou C., Karlsson A., Solaroli N.
Int. J. Biochem. Cell Biol. 45:925-931(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK057266 mRNA. Translation: BAB71402.1.
AK093446 mRNA. Translation: BAC04168.1.
AK292186 mRNA. Translation: BAF84875.1.
AL160165, AL445645 Genomic DNA. Translation: CAH70535.1.
AL445645, AL160165 Genomic DNA. Translation: CAH72109.1.
CH471090 Genomic DNA. Translation: EAW88016.1.
BC034776 mRNA. Translation: AAH34776.1.
BC050576 mRNA. Translation: AAH50576.1.
CCDSCCDS6954.1. [Q96MA6-1]
RefSeqNP_689785.1. NM_152572.2. [Q96MA6-1]
UniGeneHs.421340.

3D structure databases

ProteinModelPortalQ96MA6.
SMRQ96MA6. Positions 53-257, 270-477.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127645. 1 interaction.
IntActQ96MA6. 1 interaction.
MINTMINT-8417639.

PTM databases

PhosphoSiteQ96MA6.

Polymorphism databases

DMDM74752032.

Proteomic databases

PaxDbQ96MA6.
PRIDEQ96MA6.

Protocols and materials databases

DNASU158067.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000298545; ENSP00000298545; ENSG00000165695. [Q96MA6-1]
GeneID158067.
KEGGhsa:158067.
UCSCuc004cbu.1. human. [Q96MA6-1]

Organism-specific databases

CTD158067.
GeneCardsGC09M135601.
H-InvDBHIX0008494.
HGNCHGNC:26526. AK8.
HPAHPA021443.
HPA021445.
HPA023894.
MIM615365. gene.
neXtProtNX_Q96MA6.
PharmGKBPA134971772.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0563.
HOVERGENHBG080569.
InParanoidQ96MA6.
OMAEYHRNIV.
OrthoDBEOG71CFKV.
PhylomeDBQ96MA6.
TreeFamTF328560.

Gene expression databases

BgeeQ96MA6.
CleanExHS_C9orf98.
GenevestigatorQ96MA6.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_00235. Adenylate_kinase_Adk.
InterProIPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR23359. PTHR23359. 1 hit.
PRINTSPR00094. ADENYLTKNASE.
SUPFAMSSF52540. SSF52540. 3 hits.
SSF57774. SSF57774. 2 hits.
ProtoNetSearch...

Other

ChiTaRSAK8. human.
GenomeRNAi158067.
NextBio87622.
PROQ96MA6.
SOURCESearch...

Entry information

Entry nameKAD8_HUMAN
AccessionPrimary (citable) accession number: Q96MA6
Secondary accession number(s): A8K821, Q8N9W9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM