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Q96MA6

- KAD8_HUMAN

UniProt

Q96MA6 - KAD8_HUMAN

Protein

Adenylate kinase 8

Gene

AK8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP. Also displays broad nucleoside diphosphate kinase activity.2 Publications

    Catalytic activityi

    ATP + AMP = 2 ADP.
    ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

    Kineticsi

    1. KM=4.1 µM for AMP1 Publication
    2. KM=630 µM for dAMP1 Publication
    3. KM=1.4 µM for CMP1 Publication

    Vmax=2400 pmol/min/µg enzyme with AMP as substrate1 Publication

    Vmax=1360 pmol/min/µg enzyme with dAMP as substrate1 Publication

    Vmax=190 pmol/min/µg enzyme with CMP as substrate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei147 – 1471AMP 1By similarity
    Binding sitei203 – 2031AMP 1By similarity
    Binding sitei361 – 3611AMP 2By similarity
    Binding sitei392 – 3921ATP 2By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi67 – 726ATP 1By similarity
    Nucleotide bindingi140 – 1434AMP 1By similarity
    Nucleotide bindingi278 – 2836ATP 2By similarity
    Nucleotide bindingi325 – 3273AMP 2By similarity
    Nucleotide bindingi354 – 3574AMP 2By similarity

    GO - Molecular functioni

    1. adenylate kinase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. cytidylate kinase activity Source: UniProtKB
    4. nucleoside diphosphate kinase activity Source: UniProtKB

    GO - Biological processi

    1. nucleoside diphosphate phosphorylation Source: UniProtKB
    2. nucleoside triphosphate biosynthetic process Source: UniProtKB
    3. ventricular system development Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenylate kinase 8 (EC:2.7.4.3, EC:2.7.4.6)
    Short name:
    AK 8
    Alternative name(s):
    ATP-AMP transphosphorylase 8
    Gene namesi
    Name:AK8
    Synonyms:C9orf98
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 9

    Organism-specific databases

    HGNCiHGNC:26526. AK8.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134971772.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 479479Adenylate kinase 8PRO_0000279383Add
    BLAST

    Proteomic databases

    PaxDbiQ96MA6.
    PRIDEiQ96MA6.

    PTM databases

    PhosphoSiteiQ96MA6.

    Expressioni

    Gene expression databases

    BgeeiQ96MA6.
    CleanExiHS_C9orf98.
    GenevestigatoriQ96MA6.

    Organism-specific databases

    HPAiHPA021443.
    HPA021445.
    HPA023894.

    Interactioni

    Protein-protein interaction databases

    BioGridi127645. 1 interaction.
    IntActiQ96MA6. 1 interaction.
    MINTiMINT-8417639.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96MA6.
    SMRiQ96MA6. Positions 53-257, 270-477.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni58 – 258201Adenylate kinase 1Add
    BLAST
    Regioni87 – 11327NMPbind 1By similarityAdd
    BLAST
    Regioni177 – 20630LID 1By similarityAdd
    BLAST
    Regioni269 – 471203Adenylate kinase 2Add
    BLAST
    Regioni298 – 32730NMPbind 2By similarityAdd
    BLAST
    Regioni391 – 42434LID 2By similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the adenylate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0563.
    HOVERGENiHBG080569.
    InParanoidiQ96MA6.
    KOiK00939.
    OMAiEYHRNIV.
    OrthoDBiEOG71CFKV.
    PhylomeDBiQ96MA6.
    TreeFamiTF328560.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    HAMAPiMF_00235. Adenylate_kinase_Adk.
    InterProiIPR000850. Adenylat/UMP-CMP_kin.
    IPR007862. Adenylate_kinase_lid-dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR23359. PTHR23359. 1 hit.
    PRINTSiPR00094. ADENYLTKNASE.
    SUPFAMiSSF52540. SSF52540. 3 hits.
    SSF57774. SSF57774. 2 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q96MA6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDATIAPHRI PPEMPQYGEE NHIFELMQNM LEQLLIHQPE DPIPFMIQHL    50
    HRDNDNVPRI VILGPPASGK TTIAMWLCKH LNSSLLTLEN LILNEFSYTA 100
    TEARRLYLQR KTVPSALLVQ LIQERLAEED CIKQGWILDG IPETREQALR 150
    IQTLGITPRH VIVLSAPDTV LIERNLGKRI DPQTGEIYHT TFDWPPESEI 200
    QNRLMVPEDI SELETAQKLL EYHRNIVRVI PSYPKILKVI SADQPCVDVF 250
    YQALTYVQSN HRTNAPFTPR VLLLGPVGSG KSLQAALLAQ KYRLVNVCCG 300
    QLLKEAVADR TTFGELIQPF FEKEMAVPDS LLMKVLSQRL DQQDCIQKGW 350
    VLHGVPRDLD QAHLLNRLGY NPNRVFFLNV PFDSIMERLT LRRIDPVTGE 400
    RYHLMYKPPP TMEIQARLLQ NPKDAEEQVK LKMDLFYRNS ADLEQLYGSA 450
    ITLNGDQDPY TVFEYIESGI INPLPKKIP 479
    Length:479
    Mass (Da):54,926
    Last modified:December 1, 2001 - v1
    Checksum:i9E4EABA3F429B731
    GO
    Isoform 2 (identifier: Q96MA6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-204: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:275
    Mass (Da):31,455
    Checksum:iB90C76CF137D6C34
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti5 – 51I → T.
    Corresponds to variant rs2231400 [ dbSNP | Ensembl ].
    VAR_030873
    Natural varianti130 – 1301D → G.
    Corresponds to variant rs17407084 [ dbSNP | Ensembl ].
    VAR_030874

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 204204Missing in isoform 2. 1 PublicationVSP_023419Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK057266 mRNA. Translation: BAB71402.1.
    AK093446 mRNA. Translation: BAC04168.1.
    AK292186 mRNA. Translation: BAF84875.1.
    AL160165, AL445645 Genomic DNA. Translation: CAH70535.1.
    AL445645, AL160165 Genomic DNA. Translation: CAH72109.1.
    CH471090 Genomic DNA. Translation: EAW88016.1.
    BC034776 mRNA. Translation: AAH34776.1.
    BC050576 mRNA. Translation: AAH50576.1.
    CCDSiCCDS6954.1. [Q96MA6-1]
    RefSeqiNP_689785.1. NM_152572.2. [Q96MA6-1]
    UniGeneiHs.421340.

    Genome annotation databases

    EnsembliENST00000298545; ENSP00000298545; ENSG00000165695. [Q96MA6-1]
    GeneIDi158067.
    KEGGihsa:158067.
    UCSCiuc004cbu.1. human. [Q96MA6-1]

    Polymorphism databases

    DMDMi74752032.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK057266 mRNA. Translation: BAB71402.1 .
    AK093446 mRNA. Translation: BAC04168.1 .
    AK292186 mRNA. Translation: BAF84875.1 .
    AL160165 , AL445645 Genomic DNA. Translation: CAH70535.1 .
    AL445645 , AL160165 Genomic DNA. Translation: CAH72109.1 .
    CH471090 Genomic DNA. Translation: EAW88016.1 .
    BC034776 mRNA. Translation: AAH34776.1 .
    BC050576 mRNA. Translation: AAH50576.1 .
    CCDSi CCDS6954.1. [Q96MA6-1 ]
    RefSeqi NP_689785.1. NM_152572.2. [Q96MA6-1 ]
    UniGenei Hs.421340.

    3D structure databases

    ProteinModelPortali Q96MA6.
    SMRi Q96MA6. Positions 53-257, 270-477.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 127645. 1 interaction.
    IntActi Q96MA6. 1 interaction.
    MINTi MINT-8417639.

    PTM databases

    PhosphoSitei Q96MA6.

    Polymorphism databases

    DMDMi 74752032.

    Proteomic databases

    PaxDbi Q96MA6.
    PRIDEi Q96MA6.

    Protocols and materials databases

    DNASUi 158067.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000298545 ; ENSP00000298545 ; ENSG00000165695 . [Q96MA6-1 ]
    GeneIDi 158067.
    KEGGi hsa:158067.
    UCSCi uc004cbu.1. human. [Q96MA6-1 ]

    Organism-specific databases

    CTDi 158067.
    GeneCardsi GC09M135601.
    H-InvDB HIX0008494.
    HGNCi HGNC:26526. AK8.
    HPAi HPA021443.
    HPA021445.
    HPA023894.
    MIMi 615365. gene.
    neXtProti NX_Q96MA6.
    PharmGKBi PA134971772.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0563.
    HOVERGENi HBG080569.
    InParanoidi Q96MA6.
    KOi K00939.
    OMAi EYHRNIV.
    OrthoDBi EOG71CFKV.
    PhylomeDBi Q96MA6.
    TreeFami TF328560.

    Miscellaneous databases

    ChiTaRSi AK8. human.
    GenomeRNAii 158067.
    NextBioi 87622.
    PROi Q96MA6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q96MA6.
    CleanExi HS_C9orf98.
    Genevestigatori Q96MA6.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    HAMAPi MF_00235. Adenylate_kinase_Adk.
    InterProi IPR000850. Adenylat/UMP-CMP_kin.
    IPR007862. Adenylate_kinase_lid-dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR23359. PTHR23359. 1 hit.
    PRINTSi PR00094. ADENYLTKNASE.
    SUPFAMi SSF52540. SSF52540. 3 hits.
    SSF57774. SSF57774. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Testis.
    2. "DNA sequence and analysis of human chromosome 9."
      Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
      , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
      Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    5. "The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes."
      Panayiotou C., Solaroli N., Xu Y., Johansson M., Karlsson A.
      Biochem. J. 433:527-534(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY.
    6. "The human adenylate kinase 9 is a nucleoside mono- and diphosphate kinase."
      Amiri M., Conserva F., Panayiotou C., Karlsson A., Solaroli N.
      Int. J. Biochem. Cell Biol. 45:925-931(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiKAD8_HUMAN
    AccessioniPrimary (citable) accession number: Q96MA6
    Secondary accession number(s): A8K821, Q8N9W9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 6, 2007
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 9
      Human chromosome 9: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3