Q96MA6 (KAD8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylate kinase 8
EC=2.7.4.3

Gene names

Name:	AK8
Synonyms:	C9orf98

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adenylate kinase. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP. Ref.5
Catalytic activity	ATP + AMP = 2 ADP. Ref.5
Subcellular location	Cytoplasm › cytosol Ref.5.
Sequence similarities	Belongs to the adenylate kinase family.
Biophysicochemical properties	Kinetic parameters: KM=4.1 µM for AMP Ref.5 KM=630 µM for dAMP KM=1.4 µM for CMP Vmax=2400 pmol/min/µg enzyme with AMP as substrate Vmax=1360 pmol/min/µg enzyme with dAMP as substrate Vmax=190 pmol/min/µg enzyme with CMP as substrate

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ventricular system development Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate kinase activity Inferred from direct assay Ref.5. Source: UniProtKB cytidylate kinase activity Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96MA6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96MA6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-204: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Adenylate kinase 8		PRO_0000279383
Regions
	Nucleotide binding	275 – 282	8	ATP Potential
	Region	270 – 479	210	Adenylate kinase
Natural variations
	Alternative sequence	1 – 204	204	Missing in isoform 2.		VSP_023419
	Natural variant	5	1	I → T. Corresponds to variant rs2231400 [ dbSNP | Ensembl ].		VAR_030873
	Natural variant	130	1	D → G. Corresponds to variant rs17407084 [ dbSNP | Ensembl ].		VAR_030874

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 9E4EABA3F429B731	FASTA	479	54,926
	10 20 30 40 50 60 MDATIAPHRI PPEMPQYGEE NHIFELMQNM LEQLLIHQPE DPIPFMIQHL HRDNDNVPRI 70 80 90 100 110 120 VILGPPASGK TTIAMWLCKH LNSSLLTLEN LILNEFSYTA TEARRLYLQR KTVPSALLVQ 130 140 150 160 170 180 LIQERLAEED CIKQGWILDG IPETREQALR IQTLGITPRH VIVLSAPDTV LIERNLGKRI 190 200 210 220 230 240 DPQTGEIYHT TFDWPPESEI QNRLMVPEDI SELETAQKLL EYHRNIVRVI PSYPKILKVI 250 260 270 280 290 300 SADQPCVDVF YQALTYVQSN HRTNAPFTPR VLLLGPVGSG KSLQAALLAQ KYRLVNVCCG 310 320 330 340 350 360 QLLKEAVADR TTFGELIQPF FEKEMAVPDS LLMKVLSQRL DQQDCIQKGW VLHGVPRDLD 370 380 390 400 410 420 QAHLLNRLGY NPNRVFFLNV PFDSIMERLT LRRIDPVTGE RYHLMYKPPP TMEIQARLLQ 430 440 450 460 470 NPKDAEEQVK LKMDLFYRNS ADLEQLYGSA ITLNGDQDPY TVFEYIESGI INPLPKKIP
	Isoform 2 [UniParc]. Checksum: B90C76CF137D6C34	FASTA	275	31,455
	10 20 30 40 50 60 MVPEDISELE TAQKLLEYHR NIVRVIPSYP KILKVISADQ PCVDVFYQAL TYVQSNHRTN 70 80 90 100 110 120 APFTPRVLLL GPVGSGKSLQ AALLAQKYRL VNVCCGQLLK EAVADRTTFG ELIQPFFEKE 130 140 150 160 170 180 MAVPDSLLMK VLSQRLDQQD CIQKGWVLHG VPRDLDQAHL LNRLGYNPNR VFFLNVPFDS 190 200 210 220 230 240 IMERLTLRRI DPVTGERYHL MYKPPPTMEI QARLLQNPKD AEEQVKLKMD LFYRNSADLE 250 260 270 QLYGSAITLN GDQDPYTVFE YIESGIINPL PKKIP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Testis.
[2]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[5]	"The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes." Panayiotou C., Solaroli N., Xu Y., Johansson M., Karlsson A. Biochem. J. 433:527-534(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK057266 mRNA. Translation: BAB71402.1. AK093446 mRNA. Translation: BAC04168.1. AK292186 mRNA. Translation: BAF84875.1. AL160165, AL445645 Genomic DNA. Translation: CAH70535.1. AL445645, AL160165 Genomic DNA. Translation: CAH72109.1. CH471090 Genomic DNA. Translation: EAW88016.1. BC034776 mRNA. Translation: AAH34776.1. BC050576 mRNA. Translation: AAH50576.1.
IPI	IPI00065450. IPI00830061.
RefSeq	NP_689785.1. NM_152572.2.
UniGene	Hs.421340.
3D structure databases
HSSP	HSSP built from PDB template 1ZAK based on UniProtKB P43188.
ProteinModelPortal	Q96MA6.
ModBase	Search...
PTM databases
PhosphoSite	Q96MA6.
Polymorphism databases
DMDM	74752032.
Proteomic databases
PaxDb	Q96MA6.
PRIDE	Q96MA6.
Protocols and materials databases
DNASU	158067.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000298545; ENSP00000298545; ENSG00000165695.
GeneID	158067.
KEGG	hsa:158067.
UCSC	uc004cbu.1. human.
Organism-specific databases
CTD	158067.
GeneCards	GC09M135601.
H-InvDB	HIX0008494.
HGNC	HGNC:26526. AK8.
HPA	HPA021443. HPA021445. HPA023894.
neXtProt	NX_Q96MA6.
PharmGKB	PA134971772.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0563.
HOVERGEN	HBG080569.
InParanoid	Q96MA6.
OMA	EYHRNIV.
OrthoDB	EOG49KFQW.
PhylomeDB	Q96MA6.
Gene expression databases
Bgee	Q96MA6.
CleanEx	HS_C9orf98.
Genevestigator	Q96MA6.
Family and domain databases
InterPro	IPR000850. Adenylate_kin. IPR007862. Adenylate_kinase_lid-dom. IPR026867. AK7/AK8. [Graphical view]
PANTHER	PTHR23359. PTHR23359. 1 hit. PTHR23359:SF22. PTHR23359:SF22. 1 hit.
Pfam	PF00406. ADK. 2 hits. [Graphical view]
PRINTS	PR00094. ADENYLTKNASE.
SUPFAM	SSF57774. Adenylate_kinase_Znf_lid. 1 hit.
PROSITE	PS00113. ADENYLATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	AK8. human.
GenomeRNAi	158067.
NextBio	87622.

Entry information

Entry name

KAD8_HUMAN

Accession

Primary (citable) accession number: Q96MA6
Secondary accession number(s): A8K821, Q8N9W9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.