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Protein

Adenylate kinase 7

Gene

AK7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP. Also displays broad nucleoside diphosphate kinase activity. Involved in maintaining ciliary structure and function.2 Publications

Catalytic activityi

ATP + AMP = 2 ADP.2 Publications
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.1 Publication

Kineticsi

  1. KM=1.0 µM for AMP1 Publication
  2. KM=28 µM for dAMP1 Publication
  3. KM=1.2 µM for CMP1 Publication
  1. Vmax=1130 pmol/min/µg enzyme with AMP as substrate1 Publication
  2. Vmax=860 pmol/min/µg enzyme with dAMP as substrate1 Publication
  3. Vmax=150 pmol/min/µg enzyme with CMP as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei489AMPBy similarity1
Binding sitei555AMPBy similarity1
Binding sitei587ATP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi377 – 382ATPBy similarity6
Nucleotide bindingi432 – 455AMPBy similarityAdd BLAST24
Nucleotide bindingi482 – 485AMPBy similarity4

GO - Molecular functioni

  • adenylate kinase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • cytidylate kinase activity Source: UniProtKB
  • nucleoside diphosphate kinase activity Source: UniProtKB
  • nucleoside kinase activity Source: Reactome

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS13813-MONOMER.
ReactomeiR-HSA-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase 7 (EC:2.7.4.32 Publications, EC:2.7.4.61 Publication)
Short name:
AK 7
Alternative name(s):
ATP-AMP transphosphorylase 7
Gene namesi
Name:AK7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:20091. AK7.

Subcellular locationi

  • Cytoplasmcytosol 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi122481.
OpenTargetsiENSG00000140057.
PharmGKBiPA134963502.

Polymorphism and mutation databases

BioMutaiAK7.
DMDMi269849674.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001589521 – 723Adenylate kinase 7Add BLAST723

Proteomic databases

EPDiQ96M32.
PaxDbiQ96M32.
PeptideAtlasiQ96M32.
PRIDEiQ96M32.

PTM databases

iPTMnetiQ96M32.
PhosphoSitePlusiQ96M32.

Expressioni

Gene expression databases

BgeeiENSG00000140057.
CleanExiHS_AK7.
ExpressionAtlasiQ96M32. baseline and differential.
GenevisibleiQ96M32. HS.

Organism-specific databases

HPAiHPA003543.

Interactioni

Protein-protein interaction databases

BioGridi125773. 4 interactors.
IntActiQ96M32. 4 interactors.
STRINGi9606.ENSP00000267584.

Structurei

3D structure databases

ProteinModelPortaliQ96M32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni367 – 612Adenylate kinase1 PublicationAdd BLAST246
Regioni397 – 455NMPbindBy similarityAdd BLAST59
Regioni537 – 547LIDBy similarityAdd BLAST11
Regioni679 – 723DPY-301 PublicationAdd BLAST45

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili46 – 458Sequence analysisAdd BLAST413
Coiled coili609 – 677Sequence analysisAdd BLAST69

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 6Poly-Glu4
Compositional biasi51 – 58Poly-Glu8
Compositional biasi420 – 432Poly-GluAdd BLAST13
Compositional biasi497 – 502Poly-Glu6
Compositional biasi616 – 674Glu-richAdd BLAST59

Sequence similaritiesi

In the central section; belongs to the adenylate kinase family.Curated
In the C-terminal section; belongs to the dpy-30 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00390000015102.
HOGENOMiHOG000007764.
HOVERGENiHBG045568.
InParanoidiQ96M32.
KOiK00939.
OMAiKMAWLGE.
OrthoDBiEOG091G0MZX.
PhylomeDBiQ96M32.
TreeFamiTF313982.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 2 hits.
3.40.50.720. 1 hit.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR007858. Dpy-30_motif.
IPR016040. NAD(P)-bd_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 3 hits.
PfamiPF05186. Dpy-30. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q96M32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEEEETAAL TEKVIRTQRV FINLLDSYSS GNIGKFLSNC VVGASLEEIT
60 70 80 90 100
EEEEEEDENK SAMLEASSTK VKEGTFQIVG TLSKPDSPRP DFAVETYSAI
110 120 130 140 150
SREDLLMRLL ECDVIIYNIT ESSQQMEEAI WAVSALSEEV SHFEKRKLFI
160 170 180 190 200
LLSTVMTWAR SKALDPEDSE VPFTEEDYRR RKSHPNFLDH INAEKMVLKF
210 220 230 240 250
GKKARKFAAY VVAAGLQYGA EGGMLHTFFK MAWLGEIPAL PVFGDGTNVI
260 270 280 290 300
PTIHVLDLAG VIQNVIDHVP KPHYLVAVDE SVHTLEDIVK CISKNTGPGK
310 320 330 340 350
IQKIPRENAY LTKDLTQDCL DHLLVNLRME ALFVKENFNI RWAAQTGFVE
360 370 380 390 400
NINTILKEYK QSRGLMPIKI CILGPPAVGK SSIAKELANY YKLHHIQLKD
410 420 430 440 450
VISEAIAKLE AIVAPNDVGE GEEEVEEEEE EENVEDAQEL LDGIKESMEQ
460 470 480 490 500
NAGQLDDQYI IRFMKEKLKS MPCRNQGYIL DGFPKTYDQA KDLFNQEDEE
510 520 530 540 550
EEDDVRGRMF PFDKLIIPEF VCALDASDEF LKERVINLPE SIVAGTHYSQ
560 570 580 590 600
DRFLRALSNY RDINIDDETV FNYFDELEIH PIHIDVGKLE DAQNRLAIKQ
610 620 630 640 650
LIKEIGEPRN YGLTDEEKAE EERKAAEERL AREAAEEAER EHQEAVEMAE
660 670 680 690 700
KIARWEEWNK RLEEVKREER ELLEAQSIPL RNYLMTYVMP TLIQGLNECC
710 720
NVRPEDPVDF LAEYLFKNNP EAQ
Length:723
Mass (Da):82,658
Last modified:November 24, 2009 - v3
Checksum:i7A9E2BF11A4CEEF8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti272P → L in BAB71480 (PubMed:14702039).Curated1
Sequence conflicti410E → G in BAB71480 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_017059102R → Q.1 PublicationCorresponds to variant rs2275554dbSNPEnsembl.1
Natural variantiVAR_057950389N → K.1 PublicationCorresponds to variant rs2369679dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057426 mRNA. Translation: BAB71480.1.
AL163051 Genomic DNA. No translation available.
AL359240 Genomic DNA. No translation available.
CCDSiCCDS9945.1.
RefSeqiNP_689540.2. NM_152327.3.
UniGeneiHs.667462.

Genome annotation databases

EnsembliENST00000267584; ENSP00000267584; ENSG00000140057.
GeneIDi122481.
KEGGihsa:122481.
UCSCiuc001yfn.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057426 mRNA. Translation: BAB71480.1.
AL163051 Genomic DNA. No translation available.
AL359240 Genomic DNA. No translation available.
CCDSiCCDS9945.1.
RefSeqiNP_689540.2. NM_152327.3.
UniGeneiHs.667462.

3D structure databases

ProteinModelPortaliQ96M32.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125773. 4 interactors.
IntActiQ96M32. 4 interactors.
STRINGi9606.ENSP00000267584.

PTM databases

iPTMnetiQ96M32.
PhosphoSitePlusiQ96M32.

Polymorphism and mutation databases

BioMutaiAK7.
DMDMi269849674.

Proteomic databases

EPDiQ96M32.
PaxDbiQ96M32.
PeptideAtlasiQ96M32.
PRIDEiQ96M32.

Protocols and materials databases

DNASUi122481.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000267584; ENSP00000267584; ENSG00000140057.
GeneIDi122481.
KEGGihsa:122481.
UCSCiuc001yfn.3. human.

Organism-specific databases

CTDi122481.
DisGeNETi122481.
GeneCardsiAK7.
H-InvDBHIX0011951.
HGNCiHGNC:20091. AK7.
HPAiHPA003543.
MIMi615364. gene.
neXtProtiNX_Q96M32.
OpenTargetsiENSG00000140057.
PharmGKBiPA134963502.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00390000015102.
HOGENOMiHOG000007764.
HOVERGENiHBG045568.
InParanoidiQ96M32.
KOiK00939.
OMAiKMAWLGE.
OrthoDBiEOG091G0MZX.
PhylomeDBiQ96M32.
TreeFamiTF313982.

Enzyme and pathway databases

BioCyciZFISH:HS13813-MONOMER.
ReactomeiR-HSA-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

ChiTaRSiAK7. human.
GenomeRNAii122481.
PROiQ96M32.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000140057.
CleanExiHS_AK7.
ExpressionAtlasiQ96M32. baseline and differential.
GenevisibleiQ96M32. HS.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 2 hits.
3.40.50.720. 1 hit.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR007858. Dpy-30_motif.
IPR016040. NAD(P)-bd_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 3 hits.
PfamiPF05186. Dpy-30. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKAD7_HUMAN
AccessioniPrimary (citable) accession number: Q96M32
Secondary accession number(s): Q8IYP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: November 24, 2009
Last modified: November 30, 2016
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.