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Q96M32

- KAD7_HUMAN

UniProt

Q96M32 - KAD7_HUMAN

Protein

Adenylate kinase 7

Gene

AK7

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 3 (24 Nov 2009)
      Previous versions | rss
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    Functioni

    Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP. Also displays broad nucleoside diphosphate kinase activity. Involved in maintaining ciliary structure and function.2 Publications

    Catalytic activityi

    ATP + AMP = 2 ADP.
    ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

    Kineticsi

    1. KM=1.0 µM for AMP1 Publication
    2. KM=28 µM for dAMP1 Publication
    3. KM=1.2 µM for CMP1 Publication

    Vmax=1130 pmol/min/µg enzyme with AMP as substrate1 Publication

    Vmax=860 pmol/min/µg enzyme with dAMP as substrate1 Publication

    Vmax=150 pmol/min/µg enzyme with CMP as substrate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei489 – 4891AMPBy similarity
    Binding sitei555 – 5551AMPBy similarity
    Binding sitei587 – 5871ATP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi377 – 3826ATPBy similarity
    Nucleotide bindingi432 – 45524AMPBy similarityAdd
    BLAST
    Nucleotide bindingi482 – 4854AMPBy similarity

    GO - Molecular functioni

    1. adenylate kinase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. cytidylate kinase activity Source: UniProtKB
    4. nucleoside diphosphate kinase activity Source: UniProtKB

    GO - Biological processi

    1. brain development Source: Ensembl
    2. cell projection organization Source: UniProtKB-KW
    3. epithelial cilium movement Source: Ensembl
    4. inflammatory response to antigenic stimulus Source: Ensembl
    5. nucleoside diphosphate phosphorylation Source: UniProtKB
    6. nucleoside triphosphate biosynthetic process Source: UniProtKB
    7. spermatogenesis Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Cilium biogenesis/degradation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenylate kinase 7 (EC:2.7.4.3, EC:2.7.4.6)
    Short name:
    AK 7
    Alternative name(s):
    ATP-AMP transphosphorylase 7
    Gene namesi
    Name:AK7
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:20091. AK7.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. cytosol Source: UniProtKB-SubCell
    3. nucleus Source: HPA

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134963502.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 723723Adenylate kinase 7PRO_0000158952Add
    BLAST

    Proteomic databases

    PaxDbiQ96M32.
    PRIDEiQ96M32.

    PTM databases

    PhosphoSiteiQ96M32.

    Expressioni

    Gene expression databases

    ArrayExpressiQ96M32.
    BgeeiQ96M32.
    CleanExiHS_AK7.
    GenevestigatoriQ96M32.

    Organism-specific databases

    HPAiHPA003543.

    Interactioni

    Protein-protein interaction databases

    BioGridi125773. 1 interaction.
    STRINGi9606.ENSP00000267584.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96M32.
    SMRiQ96M32. Positions 335-517.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni367 – 612246Adenylate kinaseAdd
    BLAST
    Regioni397 – 45559NMPbindBy similarityAdd
    BLAST
    Regioni537 – 54711LIDBy similarityAdd
    BLAST
    Regioni679 – 72345DPY-30Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili46 – 458413Sequence AnalysisAdd
    BLAST
    Coiled coili609 – 67769Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi3 – 64Poly-Glu
    Compositional biasi51 – 588Poly-Glu
    Compositional biasi420 – 43213Poly-GluAdd
    BLAST
    Compositional biasi497 – 5026Poly-Glu
    Compositional biasi616 – 67459Glu-richAdd
    BLAST

    Sequence similaritiesi

    In the central section; belongs to the adenylate kinase family.Curated
    In the C-terminal section; belongs to the dpy-30 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG267062.
    HOGENOMiHOG000007764.
    HOVERGENiHBG045568.
    InParanoidiQ96M32.
    KOiK00939.
    OMAiKMAWLGE.
    OrthoDBiEOG786H2R.
    PhylomeDBiQ96M32.
    TreeFamiTF313982.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR000850. Adenylat/UMP-CMP_kin.
    IPR007858. Dpy-30_motif.
    IPR016040. NAD(P)-bd_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR23359. PTHR23359. 1 hit.
    PfamiPF05186. Dpy-30. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q96M32-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEEEETAAL TEKVIRTQRV FINLLDSYSS GNIGKFLSNC VVGASLEEIT    50
    EEEEEEDENK SAMLEASSTK VKEGTFQIVG TLSKPDSPRP DFAVETYSAI 100
    SREDLLMRLL ECDVIIYNIT ESSQQMEEAI WAVSALSEEV SHFEKRKLFI 150
    LLSTVMTWAR SKALDPEDSE VPFTEEDYRR RKSHPNFLDH INAEKMVLKF 200
    GKKARKFAAY VVAAGLQYGA EGGMLHTFFK MAWLGEIPAL PVFGDGTNVI 250
    PTIHVLDLAG VIQNVIDHVP KPHYLVAVDE SVHTLEDIVK CISKNTGPGK 300
    IQKIPRENAY LTKDLTQDCL DHLLVNLRME ALFVKENFNI RWAAQTGFVE 350
    NINTILKEYK QSRGLMPIKI CILGPPAVGK SSIAKELANY YKLHHIQLKD 400
    VISEAIAKLE AIVAPNDVGE GEEEVEEEEE EENVEDAQEL LDGIKESMEQ 450
    NAGQLDDQYI IRFMKEKLKS MPCRNQGYIL DGFPKTYDQA KDLFNQEDEE 500
    EEDDVRGRMF PFDKLIIPEF VCALDASDEF LKERVINLPE SIVAGTHYSQ 550
    DRFLRALSNY RDINIDDETV FNYFDELEIH PIHIDVGKLE DAQNRLAIKQ 600
    LIKEIGEPRN YGLTDEEKAE EERKAAEERL AREAAEEAER EHQEAVEMAE 650
    KIARWEEWNK RLEEVKREER ELLEAQSIPL RNYLMTYVMP TLIQGLNECC 700
    NVRPEDPVDF LAEYLFKNNP EAQ 723
    Length:723
    Mass (Da):82,658
    Last modified:November 24, 2009 - v3
    Checksum:i7A9E2BF11A4CEEF8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti272 – 2721P → L in BAB71480. (PubMed:14702039)Curated
    Sequence conflicti410 – 4101E → G in BAB71480. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti102 – 1021R → Q.1 Publication
    Corresponds to variant rs2275554 [ dbSNP | Ensembl ].
    VAR_017059
    Natural varianti389 – 3891N → K.1 Publication
    Corresponds to variant rs2369679 [ dbSNP | Ensembl ].
    VAR_057950

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK057426 mRNA. Translation: BAB71480.1.
    AL163051 Genomic DNA. No translation available.
    AL359240 Genomic DNA. No translation available.
    CCDSiCCDS9945.1.
    RefSeqiNP_689540.2. NM_152327.3.
    UniGeneiHs.667462.

    Genome annotation databases

    EnsembliENST00000267584; ENSP00000267584; ENSG00000140057.
    GeneIDi122481.
    KEGGihsa:122481.
    UCSCiuc001yfn.3. human.

    Polymorphism databases

    DMDMi269849674.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK057426 mRNA. Translation: BAB71480.1 .
    AL163051 Genomic DNA. No translation available.
    AL359240 Genomic DNA. No translation available.
    CCDSi CCDS9945.1.
    RefSeqi NP_689540.2. NM_152327.3.
    UniGenei Hs.667462.

    3D structure databases

    ProteinModelPortali Q96M32.
    SMRi Q96M32. Positions 335-517.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 125773. 1 interaction.
    STRINGi 9606.ENSP00000267584.

    PTM databases

    PhosphoSitei Q96M32.

    Polymorphism databases

    DMDMi 269849674.

    Proteomic databases

    PaxDbi Q96M32.
    PRIDEi Q96M32.

    Protocols and materials databases

    DNASUi 122481.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000267584 ; ENSP00000267584 ; ENSG00000140057 .
    GeneIDi 122481.
    KEGGi hsa:122481.
    UCSCi uc001yfn.3. human.

    Organism-specific databases

    CTDi 122481.
    GeneCardsi GC14P096858.
    H-InvDB HIX0011951.
    HGNCi HGNC:20091. AK7.
    HPAi HPA003543.
    MIMi 615364. gene.
    neXtProti NX_Q96M32.
    PharmGKBi PA134963502.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG267062.
    HOGENOMi HOG000007764.
    HOVERGENi HBG045568.
    InParanoidi Q96M32.
    KOi K00939.
    OMAi KMAWLGE.
    OrthoDBi EOG786H2R.
    PhylomeDBi Q96M32.
    TreeFami TF313982.

    Miscellaneous databases

    ChiTaRSi AK7. human.
    GenomeRNAii 122481.
    NextBioi 80898.
    PROi Q96M32.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q96M32.
    Bgeei Q96M32.
    CleanExi HS_AK7.
    Genevestigatori Q96M32.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR000850. Adenylat/UMP-CMP_kin.
    IPR007858. Dpy-30_motif.
    IPR016040. NAD(P)-bd_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR23359. PTHR23359. 1 hit.
    Pfami PF05186. Dpy-30. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS GLN-102 AND LYS-389.
      Tissue: Testis.
    2. "The DNA sequence and analysis of human chromosome 14."
      Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
      , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
      Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes."
      Panayiotou C., Solaroli N., Xu Y., Johansson M., Karlsson A.
      Biochem. J. 433:527-534(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY.
    4. "The human adenylate kinase 9 is a nucleoside mono- and diphosphate kinase."
      Amiri M., Conserva F., Panayiotou C., Karlsson A., Solaroli N.
      Int. J. Biochem. Cell Biol. 45:925-931(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiKAD7_HUMAN
    AccessioniPrimary (citable) accession number: Q96M32
    Secondary accession number(s): Q8IYP6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2003
    Last sequence update: November 24, 2009
    Last modified: October 1, 2014
    This is version 111 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3