Q96M32 (KAD7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylate kinase 7
EC=2.7.4.3

Gene names

Name:

AK7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adenylate kinase involved in maintaining ciliary structure and function By similarity. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP. Ref.3
Catalytic activity	ATP + AMP = 2 ADP. Ref.3
Subcellular location	Cytoplasm › cytosol Ref.3.
Sequence similarities	In the central section; belongs to the adenylate kinase family. In the C-terminal section; belongs to the dpy-30 family.
Biophysicochemical properties	Kinetic parameters: KM=1.0 µM for AMP Ref.3 KM=28 µM for dAMP KM=1.2 µM for CMP Vmax=1130 pmol/min/µg enzyme with AMP as substrate Vmax=860 pmol/min/µg enzyme with dAMP as substrate Vmax=150 pmol/min/µg enzyme with CMP as substrate

Ontologies

Keywords
Biological process	Cilium biogenesis/degradation
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	brain development Inferred from electronic annotation. Source: Compara cilium axoneme assembly Inferred from electronic annotation. Source: Compara epithelial cilium movement Inferred from electronic annotation. Source: Compara inflammatory response to antigenic stimulus Inferred from electronic annotation. Source: Compara spermatogenesis Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate kinase activity Inferred from direct assay Ref.3. Source: UniProtKB cytidylate kinase activity Inferred from direct assay Ref.3. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 723	723	Adenylate kinase 7		PRO_0000158952
Regions
	Nucleotide binding	374 – 381	8	ATP Potential
	Region	366 – 502	137	Adenylate kinase
	Region	679 – 723	45	DPY-30
	Coiled coil	46 – 458	413	Potential
	Coiled coil	609 – 677	69	Potential
	Compositional bias	3 – 6	4	Poly-Glu
	Compositional bias	51 – 58	8	Poly-Glu
	Compositional bias	420 – 432	13	Poly-Glu
	Compositional bias	497 – 502	6	Poly-Glu
	Compositional bias	616 – 674	59	Glu-rich
Natural variations
	Natural variant	102	1	R → Q. Ref.1 Corresponds to variant rs2275554 [ dbSNP | Ensembl ].		VAR_017059
	Natural variant	389	1	N → K. Ref.1 Corresponds to variant rs2369679 [ dbSNP | Ensembl ].		VAR_057950
Experimental info
	Sequence conflict	272	1	P → L in BAB71480. Ref.1
	Sequence conflict	410	1	E → G in BAB71480. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q96M32 [UniParc]. Last modified November 24, 2009. Version 3. Checksum: 7A9E2BF11A4CEEF8	FASTA	723	82,658
	10 20 30 40 50 60 MAEEEETAAL TEKVIRTQRV FINLLDSYSS GNIGKFLSNC VVGASLEEIT EEEEEEDENK 70 80 90 100 110 120 SAMLEASSTK VKEGTFQIVG TLSKPDSPRP DFAVETYSAI SREDLLMRLL ECDVIIYNIT 130 140 150 160 170 180 ESSQQMEEAI WAVSALSEEV SHFEKRKLFI LLSTVMTWAR SKALDPEDSE VPFTEEDYRR 190 200 210 220 230 240 RKSHPNFLDH INAEKMVLKF GKKARKFAAY VVAAGLQYGA EGGMLHTFFK MAWLGEIPAL 250 260 270 280 290 300 PVFGDGTNVI PTIHVLDLAG VIQNVIDHVP KPHYLVAVDE SVHTLEDIVK CISKNTGPGK 310 320 330 340 350 360 IQKIPRENAY LTKDLTQDCL DHLLVNLRME ALFVKENFNI RWAAQTGFVE NINTILKEYK 370 380 390 400 410 420 QSRGLMPIKI CILGPPAVGK SSIAKELANY YKLHHIQLKD VISEAIAKLE AIVAPNDVGE 430 440 450 460 470 480 GEEEVEEEEE EENVEDAQEL LDGIKESMEQ NAGQLDDQYI IRFMKEKLKS MPCRNQGYIL 490 500 510 520 530 540 DGFPKTYDQA KDLFNQEDEE EEDDVRGRMF PFDKLIIPEF VCALDASDEF LKERVINLPE 550 560 570 580 590 600 SIVAGTHYSQ DRFLRALSNY RDINIDDETV FNYFDELEIH PIHIDVGKLE DAQNRLAIKQ 610 620 630 640 650 660 LIKEIGEPRN YGLTDEEKAE EERKAAEERL AREAAEEAER EHQEAVEMAE KIARWEEWNK 670 680 690 700 710 720 RLEEVKREER ELLEAQSIPL RNYLMTYVMP TLIQGLNECC NVRPEDPVDF LAEYLFKNNP EAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS GLN-102 AND LYS-389. Tissue: Testis.
[2]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes." Panayiotou C., Solaroli N., Xu Y., Johansson M., Karlsson A. Biochem. J. 433:527-534(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK057426 mRNA. Translation: BAB71480.1. AL163051 Genomic DNA. No translation available. AL359240 Genomic DNA. No translation available.
IPI	IPI00065250.
RefSeq	NP_689540.2. NM_152327.3.
UniGene	Hs.667462.
3D structure databases
ProteinModelPortal	Q96M32.
SMR	Q96M32. Positions 367-548, 673-721.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000267584.
PTM databases
PhosphoSite	Q96M32.
Polymorphism databases
DMDM	269849674.
Proteomic databases
PaxDb	Q96M32.
PRIDE	Q96M32.
Protocols and materials databases
DNASU	122481.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000267584; ENSP00000267584; ENSG00000140057.
GeneID	122481.
KEGG	hsa:122481.
UCSC	uc001yfn.2. human.
Organism-specific databases
CTD	122481.
GeneCards	GC14P096858.
H-InvDB	HIX0011951.
HGNC	HGNC:20091. AK7.
HPA	HPA003543.
neXtProt	NX_Q96M32.
PharmGKB	PA134963502.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG267062.
HOGENOM	HOG000007764.
HOVERGEN	HBG045568.
InParanoid	Q96M32.
KO	K00939.
OMA	EAIVAPK.
OrthoDB	EOG4R7V9P.
PhylomeDB	Q96M32.
Gene expression databases
ArrayExpress	Q96M32.
Bgee	Q96M32.
CleanEx	HS_AK7.
Genevestigator	Q96M32.
GermOnline	ENSG00000140057. Homo sapiens.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR000850. Adenylate_kin. IPR026867. AK7/AK8. IPR007858. Dpy-30_motif. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR23359. PTHR23359. 1 hit. PTHR23359:SF22. PTHR23359:SF22. 1 hit.
Pfam	PF05186. Dpy-30. 1 hit. [Graphical view]
PROSITE	PS00113. ADENYLATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL3405.
ChiTaRS	AK7. human.
GenomeRNAi	122481.
NextBio	80898.

Entry information

Entry name

KAD7_HUMAN

Accession

Primary (citable) accession number: Q96M32
Secondary accession number(s): Q8IYP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2003
Last sequence update:	November 24, 2009
Last modified:	May 1, 2013
This is version 99 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.