Q96LW7 (BINCA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bcl10-interacting CARD protein
Short name=BinCARD

Gene names

Name:

C9orf89

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in inhibiting the effects of BCL10-induced activation of NF-kappa-B. May inhibit the phosphorylation of BCL10 in a CARD-dependent manner. Ref.4
Subunit structure	Associates with BCL10 by CARD-CARD interaction.
Subcellular location	Nucleus. Note: Co-expression with BCL10 induced translocation from nucleus to cytosol. Ref.4
Tissue specificity	Expressed in ovary, testis, placenta, skeletal muscle, kidney, lung, heart and liver (at protein level). Expressed in thymus and brain. Ref.4
Sequence similarities	Contains 1 CARD domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of I-kappaB kinase/NF-kappaB cascade Non-traceable author statement Ref.4. Source: UniProtKB
Cellular_component	cytosol Non-traceable author statement Ref.4. Source: UniProtKB nucleus Non-traceable author statement Ref.4. Source: UniProtKB
Molecular_function	CARD domain binding Non-traceable author statement Ref.4. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96LW7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96LW7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     102-228: RKFHITNHAC...NLVPTAAWRS → QNSDCTELDS...AFLADDLGGL

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 228

228

Bcl10-interacting CARD protein

PRO_0000064927

Regions

Domain

8 – 99

92

CARD

Natural variations

Alternative sequence

102 – 228

127

RKFHI…AAWRS → QNSDCTELDSGSQSGELSNR GPMSFLAGLGLAVGLALLLY CYPPDPKGLPGTRRVLGFSP VIIDRHVSRYLLAFLADDLG GL in isoform 2.

VSP_014723

Experimental info

Mutagenesis

17

1

L → A: Abolishes the NF-kappa-B inhibitory activity. Ref.4

Mutagenesis

65

1

L → A: Abolishes the NF-kappa-B inhibitory activity. Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

228

Helix Strand Turn

Details...

Helix

6 – 17

12

Helix

25 – 36

12

Beta strand

37 – 40

4

Helix

45 – 51

7

Helix

58 – 72

15

Helix

74 – 87

14

Helix

89 – 94

6

Helix

96 – 98

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 7A58F5AB69882CF8	FASTA	228	25,589
	10 20 30 40 50 60 MTDQTYCDRL VQDTPFLTGH GRLSEQQVDR IILQLNRYYP QILTNKEAEK FRNPKASLRV 70 80 90 100 110 120 RLCDLLSHLQ RSGERDCQEF YRALYIHAQP LHSRLPSRHA LRKFHITNHA CLVLARGGHP 130 140 150 160 170 180 SLPLMAWMSS MTTQVCCSPG LASPLASAPP QRPPSGPEGR VWQAQAVQML VSVSHFLPLP 190 200 210 220 PSLSHGSFHT AWGILYVHSC PSFSNLIPRG SLHVCVDSNL VPTAAWRS
	Isoform 2 [UniParc]. Checksum: 9F0FB9192B11D0EF	FASTA	183	20,626
	10 20 30 40 50 60 MTDQTYCDRL VQDTPFLTGH GRLSEQQVDR IILQLNRYYP QILTNKEAEK FRNPKASLRV 70 80 90 100 110 120 RLCDLLSHLQ RSGERDCQEF YRALYIHAQP LHSRLPSRHA LQNSDCTELD SGSQSGELSN 130 140 150 160 170 180 RGPMSFLAGL GLAVGLALLL YCYPPDPKGL PGTRRVLGFS PVIIDRHVSR YLLAFLADDL GGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[2]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Leukocyte and Skin.
[4]	"Inhibition of Bcl10-mediated activation of NF-kappa B by BinCARD, a Bcl10-interacting CARD protein." Woo H.-N., Hong G.-S., Jun J.-I., Cho D.-H., Choi H.-W., Lee H.-J., Chung C.-W., Kim I.-K., Jo D.-G., Pyo J.-O., Bertin J., Jung Y.-K. FEBS Lett. 578:239-244(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH BCL10, MUTAGENESIS OF LEU-17 AND LEU-65.
[5]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Embryonic kidney.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK057716 mRNA. Translation: BAB71550.1.
AL451065 Genomic DNA. Translation: CAI95110.1.
BC004500 mRNA. Translation: AAH04500.1.
BC038856 mRNA. Translation: AAH38856.1.

IPI

IPI00177808.
IPI00336153.

RefSeq

NP_115686.3. NM_032310.3.

UniGene

Hs.434213.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4DWN	X-ray	1.58	A/B	3-99	[»]
4FH0	X-ray	1.40	A/B	3-101	[»]

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-1215062.

PTM databases

PhosphoSite

Q96LW7.

Polymorphism databases

DMDM

71658794.

Proteomic databases

PaxDb

Q96LW7.

PeptideAtlas

Q96LW7.

PRIDE

Q96LW7.

Protocols and materials databases

DNASU

84270.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000375464; ENSP00000364613; ENSG00000165233.
ENST00000466409; ENSP00000437237; ENSG00000165233.

GeneID

84270.

KEGG

hsa:84270.

UCSC

uc004atd.3. human.

Organism-specific databases

CTD

84270.

GeneCards

GC09P095858.

HGNC

HGNC:28148. C9orf89.

HPA

HPA010921.

neXtProt

NX_Q96LW7.

PharmGKB

PA134909664.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG68992.

OMA

CCPPDPK.

PhylomeDB

Q96LW7.

Gene expression databases

Bgee

Q96LW7.

CleanEx

HS_C9orf89.

Genevestigator

Q96LW7.

GermOnline

ENSG00000165233. Homo sapiens.

Family and domain databases

PROSITE

PS50209. CARD. False negative.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

84270.

NextBio

73828.

Entry information

Entry name

BINCA_HUMAN

Accession

Primary (citable) accession number: Q96LW7
Secondary accession number(s): Q5BJH8, Q9BSY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2005
Last sequence update:	December 1, 2001
Last modified:	May 29, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.