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Q96LW7 (BINCA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bcl10-interacting CARD protein

Short name=BinCARD
Gene names
Name:C9orf89
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length228 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in inhibiting the effects of BCL10-induced activation of NF-kappa-B. May inhibit the phosphorylation of BCL10 in a CARD-dependent manner. Ref.4

Subunit structure

Associates with BCL10 by CARD-CARD interaction.

Subcellular location

Isoform 1: Nucleus. Note: Coexpression with BCL10 induced translocation from nucleus to cytosol. Ref.4 Ref.6

Isoform 2: Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein Ref.4 Ref.6.

Tissue specificity

Expressed in ovary, testis, placenta, skeletal muscle, kidney, lung, heart and liver (at protein level). Expressed in thymus and brain. Ref.4

Sequence similarities

Contains 1 CARD domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96LW7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96LW7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     102-228: RKFHITNHAC...NLVPTAAWRS → QNSDCTELDS...AFLADDLGGL
Note: Appears to be the dominant isoform in peripheral blood cell fractions. Contain a transmembrane helix.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 228228Bcl10-interacting CARD protein
PRO_0000064927

Regions

Domain8 – 9992CARD

Amino acid modifications

Disulfide bond7 ↔ 77Redox-active Ref.6

Natural variations

Alternative sequence102 – 228127RKFHI…AAWRS → QNSDCTELDSGSQSGELSNR GPMSFLAGLGLAVGLALLLY CYPPDPKGLPGTRRVLGFSP VIIDRHVSRYLLAFLADDLG GL in isoform 2.
VSP_014723

Experimental info

Mutagenesis171L → A: Abolishes the NF-kappa-B inhibitory activity. Ref.4
Mutagenesis651L → A: Abolishes the NF-kappa-B inhibitory activity. Ref.4

Secondary structure

................ 228
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 7A58F5AB69882CF8

FASTA22825,589
        10         20         30         40         50         60 
MTDQTYCDRL VQDTPFLTGH GRLSEQQVDR IILQLNRYYP QILTNKEAEK FRNPKASLRV 

        70         80         90        100        110        120 
RLCDLLSHLQ RSGERDCQEF YRALYIHAQP LHSRLPSRHA LRKFHITNHA CLVLARGGHP 

       130        140        150        160        170        180 
SLPLMAWMSS MTTQVCCSPG LASPLASAPP QRPPSGPEGR VWQAQAVQML VSVSHFLPLP 

       190        200        210        220 
PSLSHGSFHT AWGILYVHSC PSFSNLIPRG SLHVCVDSNL VPTAAWRS 

« Hide

Isoform 2 [UniParc].

Checksum: 9F0FB9192B11D0EF
Show »

FASTA18320,626

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Uterus.
[2]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Leukocyte and Skin.
[4]"Inhibition of Bcl10-mediated activation of NF-kappa B by BinCARD, a Bcl10-interacting CARD protein."
Woo H.-N., Hong G.-S., Jun J.-I., Cho D.-H., Choi H.-W., Lee H.-J., Chung C.-W., Kim I.-K., Jo D.-G., Pyo J.-O., Bertin J., Jung Y.-K.
FEBS Lett. 578:239-244(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH BCL10, MUTAGENESIS OF LEU-17 AND LEU-65.
[5]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic kidney.
[6]"The structure of the caspase recruitment domain of BinCARD reveals that all three cysteines can be oxidized."
Chen K.E., Richards A.A., Caradoc-Davies T.T., Vajjhala P.R., Robin G., Lua L.H., Hill J.M., Schroder K., Sweet M.J., Kellie S., Kobe B., Martin J.
Acta Crystallogr. D 69:774-784(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 3-101 OF MUTANT MET-16 AND MET-66, ALTERNATIVE SPLICING, DISULFIDE BOND, SUBCELLULAR LOCATION (ISOFORMS 1 AND 2).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK057716 mRNA. Translation: BAB71550.1.
AL451065 Genomic DNA. Translation: CAI95110.1.
BC004500 mRNA. Translation: AAH04500.1.
BC038856 mRNA. Translation: AAH38856.1.
CCDSCCDS6702.2. [Q96LW7-2]
RefSeqNP_115686.3. NM_032310.3. [Q96LW7-2]
UniGeneHs.434213.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4DWNX-ray1.58A/B3-99[»]
4FH0X-ray1.40A/B3-101[»]
ProteinModelPortalQ96LW7.
SMRQ96LW7. Positions 3-99.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123997. 1 interaction.
MINTMINT-1215062.

PTM databases

PhosphoSiteQ96LW7.

Polymorphism databases

DMDM71658794.

Proteomic databases

MaxQBQ96LW7.
PaxDbQ96LW7.
PeptideAtlasQ96LW7.
PRIDEQ96LW7.

Protocols and materials databases

DNASU84270.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000375464; ENSP00000364613; ENSG00000165233. [Q96LW7-2]
ENST00000466409; ENSP00000437237; ENSG00000165233. [Q96LW7-1]
GeneID84270.
KEGGhsa:84270.
UCSCuc004atd.3. human. [Q96LW7-2]

Organism-specific databases

CTD84270.
GeneCardsGC09P095858.
HGNCHGNC:28148. C9orf89.
HPAHPA010921.
HPA038297.
neXtProtNX_Q96LW7.
PharmGKBPA134909664.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG68992.
OMADRHVSRF.
OrthoDBEOG7KM5V8.
PhylomeDBQ96LW7.
TreeFamTF335747.

Gene expression databases

BgeeQ96LW7.
CleanExHS_C9orf89.
GenevestigatorQ96LW7.

Family and domain databases

ProtoNetSearch...

Other

GenomeRNAi84270.
NextBio73828.

Entry information

Entry nameBINCA_HUMAN
AccessionPrimary (citable) accession number: Q96LW7
Secondary accession number(s): Q5BJH8, Q9BSY2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 2, 2005
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM