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Protein

Bcl10-interacting CARD protein

Gene

C9orf89

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in inhibiting the effects of BCL10-induced activation of NF-kappa-B. May inhibit the phosphorylation of BCL10 in a CARD-dependent manner.1 Publication

GO - Molecular functioni

  1. CARD domain binding Source: UniProtKB

GO - Biological processi

  1. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Bcl10-interacting CARD protein
Short name:
BinCARD
Gene namesi
Name:C9orf89
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 9

Organism-specific databases

HGNCiHGNC:28148. C9orf89.

Subcellular locationi

Isoform 1 : Nucleus 1 Publication
Note: Coexpression with BCL10 induced translocation from nucleus to cytosol.
Isoform 2 : Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein 1 Publication. Mitochondrion membrane 1 Publication; Single-pass membrane protein 1 Publication

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. mitochondrial membrane Source: UniProtKB-SubCell
  5. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi17 – 171L → A: Abolishes the NF-kappa-B inhibitory activity. 1 Publication
Mutagenesisi65 – 651L → A: Abolishes the NF-kappa-B inhibitory activity. 1 Publication

Organism-specific databases

PharmGKBiPA134909664.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 228228Bcl10-interacting CARD proteinPRO_0000064927Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi7 ↔ 77Redox-active1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ96LW7.
PaxDbiQ96LW7.
PeptideAtlasiQ96LW7.
PRIDEiQ96LW7.

PTM databases

PhosphoSiteiQ96LW7.

Expressioni

Tissue specificityi

Expressed in ovary, testis, placenta, skeletal muscle, kidney, lung, heart and liver (at protein level). Expressed in thymus and brain.1 Publication

Gene expression databases

BgeeiQ96LW7.
CleanExiHS_C9orf89.
ExpressionAtlasiQ96LW7. baseline and differential.
GenevestigatoriQ96LW7.

Organism-specific databases

HPAiHPA010921.
HPA038297.

Interactioni

Subunit structurei

Associates with BCL10 by CARD-CARD interaction.

Protein-protein interaction databases

BioGridi123997. 4 interactions.
MINTiMINT-1215062.

Structurei

Secondary structure

1
228
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 1712Combined sources
Helixi25 – 3612Combined sources
Beta strandi37 – 404Combined sources
Helixi45 – 517Combined sources
Helixi58 – 7215Combined sources
Helixi74 – 8714Combined sources
Helixi89 – 946Combined sources
Helixi96 – 983Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DWNX-ray1.58A/B3-99[»]
4FH0X-ray1.40A/B3-101[»]
ProteinModelPortaliQ96LW7.
SMRiQ96LW7. Positions 3-99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 9992CARDAdd
BLAST

Sequence similaritiesi

Contains 1 CARD domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG68992.
GeneTreeiENSGT00390000016408.
InParanoidiQ96LW7.
OMAiPPDPKVL.
OrthoDBiEOG7KM5V8.
PhylomeDBiQ96LW7.
TreeFamiTF335747.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q96LW7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDQTYCDRL VQDTPFLTGH GRLSEQQVDR IILQLNRYYP QILTNKEAEK
60 70 80 90 100
FRNPKASLRV RLCDLLSHLQ RSGERDCQEF YRALYIHAQP LHSRLPSRHA
110 120 130 140 150
LRKFHITNHA CLVLARGGHP SLPLMAWMSS MTTQVCCSPG LASPLASAPP
160 170 180 190 200
QRPPSGPEGR VWQAQAVQML VSVSHFLPLP PSLSHGSFHT AWGILYVHSC
210 220
PSFSNLIPRG SLHVCVDSNL VPTAAWRS
Length:228
Mass (Da):25,589
Last modified:December 1, 2001 - v1
Checksum:i7A58F5AB69882CF8
GO
Isoform 2 (identifier: Q96LW7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     102-228: RKFHITNHAC...NLVPTAAWRS → QNSDCTELDS...AFLADDLGGL

Note: Appears to be the dominant isoform in peripheral blood cell fractions. Contain a transmembrane helix.

Show »
Length:183
Mass (Da):20,626
Checksum:i9F0FB9192B11D0EF
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei102 – 228127RKFHI…AAWRS → QNSDCTELDSGSQSGELSNR GPMSFLAGLGLAVGLALLLY CYPPDPKGLPGTRRVLGFSP VIIDRHVSRYLLAFLADDLG GL in isoform 2. 1 PublicationVSP_014723Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057716 mRNA. Translation: BAB71550.1.
AL451065 Genomic DNA. Translation: CAI95110.1.
BC004500 mRNA. Translation: AAH04500.1.
BC038856 mRNA. Translation: AAH38856.1.
CCDSiCCDS6702.2. [Q96LW7-2]
RefSeqiNP_115686.3. NM_032310.3. [Q96LW7-2]
UniGeneiHs.434213.

Genome annotation databases

EnsembliENST00000375464; ENSP00000364613; ENSG00000165233. [Q96LW7-2]
ENST00000466409; ENSP00000437237; ENSG00000165233. [Q96LW7-1]
GeneIDi84270.
KEGGihsa:84270.
UCSCiuc004atd.3. human. [Q96LW7-2]

Polymorphism databases

DMDMi71658794.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057716 mRNA. Translation: BAB71550.1.
AL451065 Genomic DNA. Translation: CAI95110.1.
BC004500 mRNA. Translation: AAH04500.1.
BC038856 mRNA. Translation: AAH38856.1.
CCDSiCCDS6702.2. [Q96LW7-2]
RefSeqiNP_115686.3. NM_032310.3. [Q96LW7-2]
UniGeneiHs.434213.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DWNX-ray1.58A/B3-99[»]
4FH0X-ray1.40A/B3-101[»]
ProteinModelPortaliQ96LW7.
SMRiQ96LW7. Positions 3-99.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123997. 4 interactions.
MINTiMINT-1215062.

PTM databases

PhosphoSiteiQ96LW7.

Polymorphism databases

DMDMi71658794.

Proteomic databases

MaxQBiQ96LW7.
PaxDbiQ96LW7.
PeptideAtlasiQ96LW7.
PRIDEiQ96LW7.

Protocols and materials databases

DNASUi84270.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000375464; ENSP00000364613; ENSG00000165233. [Q96LW7-2]
ENST00000466409; ENSP00000437237; ENSG00000165233. [Q96LW7-1]
GeneIDi84270.
KEGGihsa:84270.
UCSCiuc004atd.3. human. [Q96LW7-2]

Organism-specific databases

CTDi84270.
GeneCardsiGC09P095858.
HGNCiHGNC:28148. C9orf89.
HPAiHPA010921.
HPA038297.
neXtProtiNX_Q96LW7.
PharmGKBiPA134909664.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG68992.
GeneTreeiENSGT00390000016408.
InParanoidiQ96LW7.
OMAiPPDPKVL.
OrthoDBiEOG7KM5V8.
PhylomeDBiQ96LW7.
TreeFamiTF335747.

Miscellaneous databases

ChiTaRSiC9orf89. human.
GenomeRNAii84270.
NextBioi73828.

Gene expression databases

BgeeiQ96LW7.
CleanExiHS_C9orf89.
ExpressionAtlasiQ96LW7. baseline and differential.
GenevestigatoriQ96LW7.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Uterus.
  2. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Leukocyte and Skin.
  4. "Inhibition of Bcl10-mediated activation of NF-kappa B by BinCARD, a Bcl10-interacting CARD protein."
    Woo H.-N., Hong G.-S., Jun J.-I., Cho D.-H., Choi H.-W., Lee H.-J., Chung C.-W., Kim I.-K., Jo D.-G., Pyo J.-O., Bertin J., Jung Y.-K.
    FEBS Lett. 578:239-244(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH BCL10, MUTAGENESIS OF LEU-17 AND LEU-65.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  6. "The structure of the caspase recruitment domain of BinCARD reveals that all three cysteines can be oxidized."
    Chen K.E., Richards A.A., Caradoc-Davies T.T., Vajjhala P.R., Robin G., Lua L.H., Hill J.M., Schroder K., Sweet M.J., Kellie S., Kobe B., Martin J.
    Acta Crystallogr. D 69:774-784(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 3-101 OF MUTANT MET-16 AND MET-66, ALTERNATIVE SPLICING, DISULFIDE BOND, SUBCELLULAR LOCATION (ISOFORMS 1 AND 2).

Entry informationi

Entry nameiBINCA_HUMAN
AccessioniPrimary (citable) accession number: Q96LW7
Secondary accession number(s): Q5BJH8, Q9BSY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2005
Last sequence update: December 1, 2001
Last modified: February 4, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.