ID   UB2E2_HUMAN             Reviewed;         201 AA.
AC   Q96LR5;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   07-JUL-2009, entry version 72.
DE   RecName: Full=Ubiquitin-conjugating enzyme E2 E2;
DE            EC=6.3.2.19;
DE   AltName: Full=Ubiquitin-protein ligase E2;
DE   AltName: Full=Ubiquitin carrier protein E2;
DE   AltName: Full=UbcH8;
GN   Name=UBE2E2; Synonyms=UBCH8;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX   PubMed=9371400;
RA   Kimura M., Hattori T., Matsuda Y., Yoshioka T., Sumi N., Umeda Y.,
RA   Nakashima S., Okano Y.;
RT   "cDNA cloning, characterization, and chromosome mapping of UBE2E2
RT   encoding a human ubiquitin-conjugating E2 enzyme.";
RL   Cytogenet. Cell Genet. 78:107-111(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Prostate;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   IDENTIFICATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
RA   Colinge J., Superti-Furga G., Bennett K.L.;
RL   Submitted (OCT-2008) to UniProtKB.
CC   -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC       proteins (Probable).
CC   -!- CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP +
CC       diphosphate + protein N-ubiquityllysine.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AK057886; BAB71605.1; -; mRNA.
DR   EMBL; BC022332; AAH22332.1; -; mRNA.
DR   IPI; IPI00102173; -.
DR   RefSeq; NP_689866.1; -.
DR   UniGene; Hs.475688; -.
DR   UniGene; Hs.595802; -.
DR   PDB; 1Y6L; X-ray; 1.85 A; A/B/C=55-201.
DR   PDBsum; 1Y6L; -.
DR   PhosphoSite; Q96LR5; -.
DR   PRIDE; Q96LR5; -.
DR   Ensembl; ENSG00000182247; Homo sapiens.
DR   GeneID; 7325; -.
DR   KEGG; hsa:7325; -.
DR   UCSC; uc003ccg.1; human.
DR   GeneCards; GC03P023221; -.
DR   H-InvDB; HIX0018506; -.
DR   HGNC; HGNC:12478; UBE2E2.
DR   HPA; HPA003303; -.
DR   MIM; 602163; gene.
DR   PharmGKB; PA37128; -.
DR   HOGENOM; Q96LR5; -.
DR   HOVERGEN; Q96LR5; -.
DR   OMA; Q96LR5; NASDCSA.
DR   BRENDA; 6.3.2.19; 247.
DR   NextBio; 28662; -.
DR   ArrayExpress; Q96LR5; -.
DR   Bgee; Q96LR5; -.
DR   CleanEx; HS_UBE2E2; -.
DR   GermOnline; ENSG00000182247; Homo sapiens.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0042296; F:ISG15 ligase activity; IDA:HGNC.
DR   GO; GO:0004842; F:ubiquitin-protein ligase activity; IEA:EC.
DR   GO; GO:0032020; P:ISG15-protein conjugation; IDA:HGNC.
DR   GO; GO:0019941; P:modification-dependent protein catabolic pr...; IEA:UniProtKB-KW.
DR   GO; GO:0051246; P:regulation of protein metabolic process; IEA:InterPro.
DR   InterPro; IPR016135; UBQ-conjugat/RWD-like.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   Gene3D; G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.
DR   Pfam; PF00179; UQ_con; 1.
DR   ProDom; PD000461; UBQ_conjugat; 1.
DR   SMART; SM00212; UBCc; 1.
DR   PROSITE; PS00183; UBIQUITIN_CONJUGAT_1; 1.
DR   PROSITE; PS50127; UBIQUITIN_CONJUGAT_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Complete proteome; Ligase;
KW   Nucleotide-binding; Ubl conjugation pathway.
FT   CHAIN         1    201       Ubiquitin-conjugating enzyme E2 E2.
FT                                /FTId=PRO_0000082472.
FT   ACT_SITE    139    139       Glycyl thioester intermediate (By
FT                                similarity).
FT   HELIX        55     69
FT   STRAND       75     82
FT   STRAND       86     92
FT   TURN         98    101
FT   STRAND      103    109
FT   TURN        112    115
FT   STRAND      120    125
FT   HELIX       141    143
FT   TURN        144    146
FT   HELIX       153    165
FT   HELIX       175    183
FT   HELIX       185    199
SQ   SEQUENCE   201 AA;  22255 MW;  3286FB51E2B9CD3E CRC64;
     MSTEAQRVDD SPSTSGGSSD GDQRESVQQE PEREQVQPKK KEGKISSKTA AKLSTSAKRI
     QKELAEITLD PPPNCSAGPK GDNIYEWRST ILGPPGSVYE GGVFFLDITF SPDYPFKPPK
     VTFRTRIYHC NINSQGVICL DILKDNWSPA LTISKVLLSI CSLLTDCNPA DPLVGSIATQ
     YMTNRAEHDR MARQWTKRYA T
//