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Protein

Ubiquitin-conjugating enzyme E2 E2

Gene

UBE2E2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Catalyzes the ISGylation of influenza A virus NS1 protein.3 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei139Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ISG15 transferase activity Source: HGNC
  • ubiquitin conjugating enzyme activity Source: MGI
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • cellular response to DNA damage stimulus Source: MGI
  • ISG15-protein conjugation Source: HGNC
  • positive regulation of G1/S transition of mitotic cell cycle Source: MGI
  • protein K11-linked ubiquitination Source: UniProtKB
  • protein K48-linked ubiquitination Source: UniProtKB
  • protein K63-linked ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS11981-MONOMER.
BRENDAi2.3.2.B6. 2681.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ96LR5.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 E2 (EC:2.3.2.231 Publication)
Alternative name(s):
E2 ubiquitin-conjugating enzyme E2
UbcH8
Ubiquitin carrier protein E2
Ubiquitin-protein ligase E2
Gene namesi
Name:UBE2E2
Synonyms:UBCH8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:12478. UBE2E2.

Pathology & Biotechi

Organism-specific databases

DisGeNETi7325.
OpenTargetsiENSG00000182247.
PharmGKBiPA37128.

Polymorphism and mutation databases

BioMutaiUBE2E2.
DMDMi47606201.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000824722 – 201Ubiquitin-conjugating enzyme E2 E2Add BLAST200

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei11PhosphoserineCombined sources1
Modified residuei15PhosphoserineCombined sources1
Modified residuei18PhosphoserineCombined sources1
Modified residuei19PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ96LR5.
MaxQBiQ96LR5.
PaxDbiQ96LR5.
PeptideAtlasiQ96LR5.
PRIDEiQ96LR5.

PTM databases

iPTMnetiQ96LR5.
PhosphoSitePlusiQ96LR5.

Expressioni

Gene expression databases

BgeeiENSG00000182247.
CleanExiHS_UBE2E2.
ExpressionAtlasiQ96LR5. baseline and differential.
GenevisibleiQ96LR5. HS.

Organism-specific databases

HPAiCAB073417.
HPA028872.
HPA030445.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ARRDC3Q96B675EBI-2129763,EBI-2875665
DAZAP2Q150383EBI-2129763,EBI-724310
FAM9BQ8IZU03EBI-2129763,EBI-10175124
MID1O153447EBI-2129763,EBI-2340316
MID2Q9UJV3-25EBI-2129763,EBI-10172526
ospGQ99PZ62EBI-2129763,EBI-9316527From a different organism.
RNF185Q96GF13EBI-2129763,EBI-2340249
RNF5Q999427EBI-2129763,EBI-348482
TRIM39Q9HCM95EBI-2129763,EBI-739510

Protein-protein interaction databases

BioGridi113173. 72 interactors.
DIPiDIP-52704N.
IntActiQ96LR5. 49 interactors.
STRINGi9606.ENSP00000379931.

Structurei

Secondary structure

1201
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi55 – 69Combined sources15
Beta strandi75 – 82Combined sources8
Beta strandi86 – 92Combined sources7
Turni98 – 101Combined sources4
Beta strandi103 – 109Combined sources7
Turni112 – 115Combined sources4
Beta strandi120 – 125Combined sources6
Helixi141 – 143Combined sources3
Turni144 – 146Combined sources3
Helixi153 – 165Combined sources13
Helixi175 – 183Combined sources9
Helixi185 – 199Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y6LX-ray1.85A/B/C55-201[»]
ProteinModelPortaliQ96LR5.
SMRiQ96LR5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96LR5.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00760000119012.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ96LR5.
KOiK20217.
OMAiCNINSAG.
OrthoDBiEOG091G0GF8.
PhylomeDBiQ96LR5.
TreeFamiTF101117.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96LR5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTEAQRVDD SPSTSGGSSD GDQRESVQQE PEREQVQPKK KEGKISSKTA
60 70 80 90 100
AKLSTSAKRI QKELAEITLD PPPNCSAGPK GDNIYEWRST ILGPPGSVYE
110 120 130 140 150
GGVFFLDITF SPDYPFKPPK VTFRTRIYHC NINSQGVICL DILKDNWSPA
160 170 180 190 200
LTISKVLLSI CSLLTDCNPA DPLVGSIATQ YMTNRAEHDR MARQWTKRYA

T
Length:201
Mass (Da):22,255
Last modified:December 1, 2001 - v1
Checksum:i3286FB51E2B9CD3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057886 mRNA. Translation: BAB71605.1.
BC022332 mRNA. Translation: AAH22332.1.
CCDSiCCDS2637.1.
RefSeqiNP_689866.1. NM_152653.3.
XP_005265489.1. XM_005265432.2.
UniGeneiHs.475688.
Hs.595802.

Genome annotation databases

EnsembliENST00000396703; ENSP00000379931; ENSG00000182247.
ENST00000425792; ENSP00000401053; ENSG00000182247.
GeneIDi7325.
KEGGihsa:7325.
UCSCiuc003ccg.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057886 mRNA. Translation: BAB71605.1.
BC022332 mRNA. Translation: AAH22332.1.
CCDSiCCDS2637.1.
RefSeqiNP_689866.1. NM_152653.3.
XP_005265489.1. XM_005265432.2.
UniGeneiHs.475688.
Hs.595802.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y6LX-ray1.85A/B/C55-201[»]
ProteinModelPortaliQ96LR5.
SMRiQ96LR5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113173. 72 interactors.
DIPiDIP-52704N.
IntActiQ96LR5. 49 interactors.
STRINGi9606.ENSP00000379931.

PTM databases

iPTMnetiQ96LR5.
PhosphoSitePlusiQ96LR5.

Polymorphism and mutation databases

BioMutaiUBE2E2.
DMDMi47606201.

Proteomic databases

EPDiQ96LR5.
MaxQBiQ96LR5.
PaxDbiQ96LR5.
PeptideAtlasiQ96LR5.
PRIDEiQ96LR5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000396703; ENSP00000379931; ENSG00000182247.
ENST00000425792; ENSP00000401053; ENSG00000182247.
GeneIDi7325.
KEGGihsa:7325.
UCSCiuc003ccg.3. human.

Organism-specific databases

CTDi7325.
DisGeNETi7325.
GeneCardsiUBE2E2.
HGNCiHGNC:12478. UBE2E2.
HPAiCAB073417.
HPA028872.
HPA030445.
MIMi602163. gene.
neXtProtiNX_Q96LR5.
OpenTargetsiENSG00000182247.
PharmGKBiPA37128.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00760000119012.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ96LR5.
KOiK20217.
OMAiCNINSAG.
OrthoDBiEOG091G0GF8.
PhylomeDBiQ96LR5.
TreeFamiTF101117.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:HS11981-MONOMER.
BRENDAi2.3.2.B6. 2681.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ96LR5.

Miscellaneous databases

ChiTaRSiUBE2E2. human.
EvolutionaryTraceiQ96LR5.
GeneWikiiUBE2E2.
GenomeRNAii7325.
PROiQ96LR5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000182247.
CleanExiHS_UBE2E2.
ExpressionAtlasiQ96LR5. baseline and differential.
GenevisibleiQ96LR5. HS.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUB2E2_HUMAN
AccessioniPrimary (citable) accession number: Q96LR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.