Q96LC9 (BMF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bcl-2-modifying factor

Gene names

Name:

BMF

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in apoptosis. Isoform 1 seems to be the main initiator.
Subunit structure	Interacts with MCL1, BCL2, BCL2L1/BCL-Xl, BCL2A1 and BCL2L2/BCL-w. Interacts with the myosin V actin motor complex through its binding to DLC2 By similarity.
Tissue specificity	Isoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells. Ref.2
Sequence similarities	Belongs to the Bcl-2 family.
Sequence caution	The sequence AAH60783.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB15762.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Apoptosis
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of pro-apoptotic gene products Traceable author statement. Source: Reactome induction of apoptosis by intracellular signals Traceable author statement. Source: Reactome positive regulation of protein homooligomerization Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of release of cytochrome c from mitochondria Inferred from sequence or structural similarity. Source: BHF-UCL
Cellular component	cytosol Traceable author statement. Source: Reactome mitochondrial outer membrane Traceable author statement. Source: Reactome myosin complex Inferred from sequence or structural similarity Ref.1. Source: UniProtKB plasma membrane Traceable author statement. Source: Reactome
Molecular function	protein binding Inferred from physical interaction Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96LC9-1)

Also known as: BMF-I;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96LC9-2)

Also known as: BMF-II;

The sequence of this isoform differs from the canonical sequence as follows:
     131-151: Missing.

Isoform 3 (identifier: Q96LC9-3)

Also known as: BMF-III;

The sequence of this isoform differs from the canonical sequence as follows:
     98-129: GNAGYRLPLPASFPAVLPIGEQPPEGQWQHQA → APAEPKSCVVADPPLPAQPCFEWRREQERGRP
     130-184: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 184	184	Bcl-2-modifying factor		PRO_0000143111
Regions
	Region	67 – 75	9	Interaction with DLC2 By similarity
	Motif	133 – 147	15	BH3
Natural variations
	Alternative sequence	98 – 129	32	GNAGY…WQHQA → APAEPKSCVVADPPLPAQPC FEWRREQERGRP in isoform 3.		VSP_012293
	Alternative sequence	130 – 184	55	Missing in isoform 3.		VSP_012294
	Alternative sequence	131 – 151	21	Missing in isoform 2.		VSP_012292
Experimental info
	Sequence conflict	61	1	R → Q in AAH69328. Ref.4
	Sequence conflict	80	1	Q → P in AAH69505. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (BMF-I) [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 21479B25CC727853	FASTA	184	20,508
	10 20 30 40 50 60 MEPSQCVEEL EDDVFQPEDG EPVTQPGSLL SADLFAQSLL DCPLSRLQLF PLTHCCGPGL 70 80 90 100 110 120 RPTSQEDKAT QTLSPASPSQ GVMLPCGVTE EPQRLFYGNA GYRLPLPASF PAVLPIGEQP 130 140 150 160 170 180 PEGQWQHQAE VQIARKLQCI ADQFHRLHVQ QHQQNQNRVW WQILLFLHNL ALNGEENRNG AGPR
	Isoform 2 (BMF-II) [UniParc]. Checksum: 842BA90CD323E4A3	FASTA	163	17,994
	10 20 30 40 50 60 MEPSQCVEEL EDDVFQPEDG EPVTQPGSLL SADLFAQSLL DCPLSRLQLF PLTHCCGPGL 70 80 90 100 110 120 RPTSQEDKAT QTLSPASPSQ GVMLPCGVTE EPQRLFYGNA GYRLPLPASF PAVLPIGEQP 130 140 150 160 PEGQWQHQAE HQQNQNRVWW QILLFLHNLA LNGEENRNGA GPR
	Isoform 3 (BMF-III) [UniParc]. Checksum: 4147FE8EB6E14B25	FASTA	129	14,150
	10 20 30 40 50 60 MEPSQCVEEL EDDVFQPEDG EPVTQPGSLL SADLFAQSLL DCPLSRLQLF PLTHCCGPGL 70 80 90 100 110 120 RPTSQEDKAT QTLSPASPSQ GVMLPCGVTE EPQRLFYAPA EPKSCVVADP PLPAQPCFEW RREQERGRP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis." Puthalakath H., Villunger A., O'Reilly L.A., Beaumont J.G., Coultas L., Cheney R.E., Huang D.C.S., Strasser A. Science 293:1829-1832(2001) [PubMed: 11546872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: T-cell.
[2]	"Expression and transcriptional regulation of functionally distinct Bmf isoforms in B-chronic lymphocytic leukemia cells." Morales A.A., Olsson A., Celsing F., Oesterborg A., Jondal M., Osorio L.M. Leukemia 18:41-47(2004) [PubMed: 14574334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY. Tissue: B-cell.
[3]	"Characterization of long cDNA clones from human adult spleen." Hattori A., Okumura K., Nagase T., Kikuno R., Hirosawa M., Ohara O. DNA Res. 7:357-366(2000) [PubMed: 11214971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Spleen.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY029254 mRNA. Translation: AAK38748.1. AY222040 mRNA. Translation: AAP22965.1. AY222041 mRNA. Translation: AAP22966.1. AK024472 mRNA. Translation: BAB15762.1. Different initiation. BC060783 mRNA. Translation: AAH60783.1. Different initiation. BC069328 mRNA. Translation: AAH69328.1. BC069505 mRNA. Translation: AAH69505.1. BC070043 mRNA. Translation: AAH70043.2. BC104983 mRNA. Translation: AAI04984.1. BC104985 mRNA. Translation: AAI04986.1.
IPI	IPI00385530. IPI00385531. IPI00477176.
RefSeq	NP_001003940.1. NM_001003940.1. NP_001003942.1. NM_001003942.1. NP_001003943.1. NM_001003943.1. NP_277038.1. NM_033503.3.
UniGene	Hs.591104.
3D structure databases
ProteinModelPortal	Q96LC9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96LC9. 3 interactions.
STRING	Q96LC9.
PTM databases
PhosphoSite	Q96LC9.
Proteomic databases
PRIDE	Q96LC9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000354670; ENSP00000346697; ENSG00000104081. ENST00000397573; ENSP00000380703; ENSG00000104081.
GeneID	90427.
KEGG	hsa:90427.
UCSC	uc001zkt.1. human. uc001zku.1. human. uc001zkv.1. human.
Organism-specific databases
CTD	90427.
GeneCards	GC15M040380.
H-InvDB	HIX0012120.
HGNC	HGNC:24132. BMF.
HPA	HPA010120.
MIM	606266. gene.
neXtProt	NX_Q96LC9.
PharmGKB	PA134893658.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG19539.
GeneTree	ENSGT00390000017896.
HOVERGEN	HBG050697.
InParanoid	Q96LC9.
OMA	FHRLHMQ.
OrthoDB	EOG4FR0SM.
Enzyme and pathway databases
Reactome	REACT_578. Apoptosis.
Gene expression databases
ArrayExpress	Q96LC9.
Bgee	Q96LC9.
CleanEx	HS_BMF.
Genevestigator	Q96LC9.
GermOnline	ENSG00000104081. Homo sapiens.
Family and domain databases
PROSITE	PS50062. BCL2_FAMILY. False negative. PS01259. BH3. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	76756.
SOURCE	Search...

Entry information

Entry name

BMF_HUMAN

Accession

Primary (citable) accession number: Q96LC9
Secondary accession number(s): Q2M396 , Q6NT30, Q6NT56, Q6P9F6, Q7Z7D4, Q7Z7D5, Q9H7K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.