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Protein

Intraflagellar transport protein 74 homolog

Gene

IFT74

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the intraflagellar transport (IFT) complex B: together with IFT81, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds beta-tubulin via its basic region. Required for ciliogenesis.1 Publication

GO - Molecular functioni

  • beta-tubulin binding Source: UniProtKB
  • chromatin binding Source: Ensembl

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Enzyme and pathway databases

ReactomeiR-HSA-5620924. Intraflagellar transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Intraflagellar transport protein 74 homolog
Alternative name(s):
Capillary morphogenesis gene 1 protein
Short name:
CMG-1
Coiled-coil domain-containing protein 2
Gene namesi
Name:IFT74
Synonyms:CCDC2, CMG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:21424. IFT74.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasmic vesicle

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134976961.

Polymorphism and mutation databases

BioMutaiIFT74.
DMDMi50400610.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 600600Intraflagellar transport protein 74 homologPRO_0000084169Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei73 – 731PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ96LB3.
MaxQBiQ96LB3.
PaxDbiQ96LB3.
PeptideAtlasiQ96LB3.
PRIDEiQ96LB3.

PTM databases

iPTMnetiQ96LB3.
PhosphoSiteiQ96LB3.

Expressioni

Tissue specificityi

Highly expressed in adult and fetal kidney and expressed at lower level in adult heart, placenta, lung, liver and pancreas, and in fetal heart, lung and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus and spleen.1 Publication

Gene expression databases

BgeeiQ96LB3.
CleanExiHS_IFT74.
ExpressionAtlasiQ96LB3. baseline and differential.
GenevisibleiQ96LB3. HS.

Organism-specific databases

HPAiHPA020247.
HPA026684.

Interactioni

Subunit structurei

Component of the IFT complex B, at least composed of IFT20, IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT80, IFT81, and IFT88. Interacts with IFT81; the interaction is direct: within the IFT complex B, IFT74 and IFT81 mediate the transport of tubulin within the cilium. Interacts (via basic region) with beta-tubulin (via acidic region); interaction is direct. Interacts with ARL13B and IFT88.3 Publications

GO - Molecular functioni

  • beta-tubulin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi123156. 42 interactions.
IntActiQ96LB3. 15 interactions.
STRINGi9606.ENSP00000369402.

Structurei

3D structure databases

ProteinModelPortaliQ96LB3.
SMRiQ96LB3. Positions 99-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9090Basic regionAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili98 – 482385Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the IFT74 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFCX. Eukaryota.
ENOG410XWAZ. LUCA.
GeneTreeiENSGT00390000007109.
HOGENOMiHOG000031494.
HOVERGENiHBG050820.
InParanoidiQ96LB3.
KOiK19679.
OMAiDYRPIMK.
OrthoDBiEOG7GTT3K.
PhylomeDBiQ96LB3.
TreeFamiTF318352.

Family and domain databases

InterProiIPR029602. IFT74.
[Graphical view]
PANTHERiPTHR31432:SF0. PTHR31432:SF0. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96LB3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASNHKSSAA RPVSRGGVGL TGRPPSGIRP LSGNIRVATA MPPGTARPGS
60 70 80 90 100
RGCPIGTGGV LSSQIKVAHR PVTQQGLTGM KTGTKGPQRQ ILDKSYYLGL
110 120 130 140 150
LRSKISELTT EVNKLQKGIE MYNQENSVYL SYEKRAETLA VEIKELQGQL
160 170 180 190 200
ADYNMLVDKL NTNTEMEEVM NDYNMLKAQN DRETQSLDVI FTERQAKEKQ
210 220 230 240 250
IRSVEEEIEQ EKQATDDIIK NMSFENQVKY LEMKTTNEKL LQELDTLQQQ
260 270 280 290 300
LDSQNMKKES LEAEIAHSQV KQEAVLLHEK LYELESHRDQ MIAEDKSIGS
310 320 330 340 350
PMEEREKLLK QIKDDNQEIA SMERQLTDTK EKINQFIEEI RQLDMDLEEH
360 370 380 390 400
QGEMNQKYKE LKKREEHMDT FIETFEETKN QELKRKAQIE ANIVALLEHC
410 420 430 440 450
SRNINRIEQI SSITNQELKM MQDDLNFKST EVQKSQSTAQ NLTSDIQRLQ
460 470 480 490 500
LDLQKMELLE SKMTEEQHSL KSKIKQMTTD LEIYNDLPAL KSSGEEKIKK
510 520 530 540 550
LHQERMILST HRNAFKKIME KQNIEYEALK TQLQENETHS QLTNLERKWQ
560 570 580 590 600
HLEQNNFAMK EFIATKSQES DYQPIKKNVT KQIAEYNKTI VDALHSTSGN
Length:600
Mass (Da):69,239
Last modified:December 1, 2001 - v1
Checksum:i01366A697500D52D
GO
Isoform 2 (identifier: Q96LB3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     352-600: GEMNQKYKEL...VDALHSTSGN → DPTNYGWKILEKNTGSFKKQV

Show »
Length:372
Mass (Da):42,332
Checksum:i339DC80C2F9E9ED7
GO

Sequence cautioni

The sequence BAB14650.1 differs from that shown. Reason: Erroneous termination at position 159. Translated as Lys.Curated
The sequence BAB15423.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti172 – 1721D → G in BAB14650 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti55 – 551I → M.
Corresponds to variant rs10812505 [ dbSNP | Ensembl ].
VAR_051062
Natural varianti85 – 851K → R.
Corresponds to variant rs11555693 [ dbSNP | Ensembl ].
VAR_061667
Natural varianti110 – 1101T → A.
Corresponds to variant rs12004404 [ dbSNP | Ensembl ].
VAR_051063
Natural varianti224 – 2241F → L.1 Publication
Corresponds to variant rs17694549 [ dbSNP | Ensembl ].
VAR_051064
Natural varianti355 – 3551N → T.
Corresponds to variant rs34628525 [ dbSNP | Ensembl ].
VAR_051065
Natural varianti597 – 5971T → I.1 Publication
Corresponds to variant rs3429 [ dbSNP | Ensembl ].
VAR_051066

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei352 – 600249GEMNQ…STSGN → DPTNYGWKILEKNTGSFKKQ V in isoform 2. 2 PublicationsVSP_041328Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY040325 mRNA. Translation: AAK77221.1.
AK023707 mRNA. Translation: BAB14650.1. Sequence problems.
AK026274 mRNA. Translation: BAB15423.1. Different initiation.
AL356133, AL355432 Genomic DNA. Translation: CAH72643.1.
CH471071 Genomic DNA. Translation: EAW58573.1.
BC107742 mRNA. Translation: AAI07743.1.
BX436367 mRNA. No translation available.
CCDSiCCDS43793.1. [Q96LB3-1]
CCDS47955.1. [Q96LB3-2]
RefSeqiNP_001092692.1. NM_001099222.1. [Q96LB3-1]
NP_001092693.1. NM_001099223.1. [Q96LB3-1]
NP_001092694.1. NM_001099224.1. [Q96LB3-2]
NP_079379.2. NM_025103.2. [Q96LB3-1]
UniGeneiHs.145402.

Genome annotation databases

EnsembliENST00000380062; ENSP00000369402; ENSG00000096872. [Q96LB3-1]
ENST00000429045; ENSP00000393907; ENSG00000096872. [Q96LB3-2]
ENST00000433700; ENSP00000389224; ENSG00000096872. [Q96LB3-1]
ENST00000443698; ENSP00000404122; ENSG00000096872. [Q96LB3-1]
GeneIDi80173.
KEGGihsa:80173.
UCSCiuc003zqf.5. human. [Q96LB3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY040325 mRNA. Translation: AAK77221.1.
AK023707 mRNA. Translation: BAB14650.1. Sequence problems.
AK026274 mRNA. Translation: BAB15423.1. Different initiation.
AL356133, AL355432 Genomic DNA. Translation: CAH72643.1.
CH471071 Genomic DNA. Translation: EAW58573.1.
BC107742 mRNA. Translation: AAI07743.1.
BX436367 mRNA. No translation available.
CCDSiCCDS43793.1. [Q96LB3-1]
CCDS47955.1. [Q96LB3-2]
RefSeqiNP_001092692.1. NM_001099222.1. [Q96LB3-1]
NP_001092693.1. NM_001099223.1. [Q96LB3-1]
NP_001092694.1. NM_001099224.1. [Q96LB3-2]
NP_079379.2. NM_025103.2. [Q96LB3-1]
UniGeneiHs.145402.

3D structure databases

ProteinModelPortaliQ96LB3.
SMRiQ96LB3. Positions 99-150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123156. 42 interactions.
IntActiQ96LB3. 15 interactions.
STRINGi9606.ENSP00000369402.

PTM databases

iPTMnetiQ96LB3.
PhosphoSiteiQ96LB3.

Polymorphism and mutation databases

BioMutaiIFT74.
DMDMi50400610.

Proteomic databases

EPDiQ96LB3.
MaxQBiQ96LB3.
PaxDbiQ96LB3.
PeptideAtlasiQ96LB3.
PRIDEiQ96LB3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000380062; ENSP00000369402; ENSG00000096872. [Q96LB3-1]
ENST00000429045; ENSP00000393907; ENSG00000096872. [Q96LB3-2]
ENST00000433700; ENSP00000389224; ENSG00000096872. [Q96LB3-1]
ENST00000443698; ENSP00000404122; ENSG00000096872. [Q96LB3-1]
GeneIDi80173.
KEGGihsa:80173.
UCSCiuc003zqf.5. human. [Q96LB3-1]

Organism-specific databases

CTDi80173.
GeneCardsiIFT74.
HGNCiHGNC:21424. IFT74.
HPAiHPA020247.
HPA026684.
MIMi608040. gene.
neXtProtiNX_Q96LB3.
PharmGKBiPA134976961.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFCX. Eukaryota.
ENOG410XWAZ. LUCA.
GeneTreeiENSGT00390000007109.
HOGENOMiHOG000031494.
HOVERGENiHBG050820.
InParanoidiQ96LB3.
KOiK19679.
OMAiDYRPIMK.
OrthoDBiEOG7GTT3K.
PhylomeDBiQ96LB3.
TreeFamiTF318352.

Enzyme and pathway databases

ReactomeiR-HSA-5620924. Intraflagellar transport.

Miscellaneous databases

ChiTaRSiIFT74. human.
GeneWikiiIFT74.
GenomeRNAii80173.
PROiQ96LB3.
SOURCEiSearch...

Gene expression databases

BgeeiQ96LB3.
CleanExiHS_IFT74.
ExpressionAtlasiQ96LB3. baseline and differential.
GenevisibleiQ96LB3. HS.

Family and domain databases

InterProiIPR029602. IFT74.
[Graphical view]
PANTHERiPTHR31432:SF0. PTHR31432:SF0. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Differential gene expression during capillary morphogenesis in 3D collagen matrices: regulated expression of genes involved in basement membrane matrix assembly, cell cycle progression, cellular differentiation and G-protein signaling."
    Bell S.E., Mavila A., Salazar R., Bayless K.J., Kanagala S., Maxwell S.A., Davis G.E.
    J. Cell Sci. 114:2755-2773(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Tissue: Umbilical vein.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS LEU-224 AND ILE-597.
    Tissue: Placenta.
  3. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  6. "Full-length cDNA libraries and normalization."
    Li W.B., Gruber C., Jessee J., Polayes D.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 197-372 (ISOFORM 2).
    Tissue: Thymus.
  7. "Primary cilia of human endothelial cells disassemble under laminar shear stress."
    Iomini C., Tejada K., Mo W., Vaananen H., Piperno G.
    J. Cell Biol. 164:811-817(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Characterization of the intraflagellar transport complex B core: direct interaction of the IFT81 and IFT74/72 subunits."
    Lucker B.F., Behal R.H., Qin H., Siron L.C., Taggart W.D., Rosenbaum J.L., Cole D.G.
    J. Biol. Chem. 280:27688-27696(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IFT81.
  9. Cited for: INTERACTION WITH ARL13B.
  10. "Molecular basis of tubulin transport within the cilium by IFT74 and IFT81."
    Bhogaraju S., Cajanek L., Fort C., Blisnick T., Weber K., Taschner M., Mizuno N., Lamla S., Bastin P., Nigg E.A., Lorentzen E.
    Science 341:1009-1012(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE IFT COMPLEX B, INTERACTION WITH BETA-TUBULIN AND IFT81.

Entry informationi

Entry nameiIFT74_HUMAN
AccessioniPrimary (citable) accession number: Q96LB3
Secondary accession number(s): Q3B789
, Q5VY34, Q6PGQ8, Q9H643, Q9H8G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 1, 2001
Last modified: July 6, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.