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Protein

Mas-related G-protein coupled receptor member X1

Gene

MRGPRX1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells. G(alpha)q proteins are involved in the calcium-signaling pathway. Activated by the antimalarial drug, chloroquine. May mediate chloroquine-induced itch, in a histamine-independent manner.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Acute phase

Names & Taxonomyi

Protein namesi
Recommended name:
Mas-related G-protein coupled receptor member X1
Alternative name(s):
Sensory neuron-specific G-protein coupled receptor 3/4
Gene namesi
Name:MRGPRX1
Synonyms:MRGX1, SNSR3, SNSR4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:17962. MRGPRX1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131ExtracellularSequence AnalysisAdd
BLAST
Transmembranei32 – 5221Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini53 – 6715CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei68 – 8821Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini89 – 968ExtracellularSequence Analysis
Transmembranei97 – 11721Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini118 – 14427CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei145 – 16521Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini166 – 17712ExtracellularSequence AnalysisAdd
BLAST
Transmembranei178 – 19821Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini199 – 22123CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei222 – 24221Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini243 – 25412ExtracellularSequence AnalysisAdd
BLAST
Transmembranei255 – 27521Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini276 – 32247CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671334.

Polymorphism and mutation databases

BioMutaiMRGPRX1.
DMDMi50401128.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Mas-related G-protein coupled receptor member X1PRO_0000069772Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi16 – 161N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ96LB2.

PTM databases

PhosphoSiteiQ96LB2.

Expressioni

Tissue specificityi

Uniquely localized in a subset of small dorsal root and trigeminal sensory neurons.1 Publication

Gene expression databases

BgeeiQ96LB2.
CleanExiHS_MRGPRX1.
ExpressionAtlasiQ96LB2. baseline.
GenevisibleiQ96LB2. HS.

Interactioni

Protein-protein interaction databases

BioGridi129233. 4 interactions.
STRINGi9606.ENSP00000305766.

Structurei

3D structure databases

ProteinModelPortaliQ96LB2.
SMRiQ96LB2. Positions 28-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Mas subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG146549.
GeneTreeiENSGT00550000074531.
HOGENOMiHOG000015412.
HOVERGENiHBG003592.
InParanoidiQ96LB2.
KOiK08396.
OMAiSAWCQTS.
OrthoDBiEOG75MVX4.
PhylomeDBiQ96LB2.
TreeFamiTF336336.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR026234. MRGPCRFAMILY.
[Graphical view]
PANTHERiPTHR11334. PTHR11334. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR02108. MRGPCRFAMILY.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96LB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPTISTLDT ELTPINGTEE TLCYKQTLSL TVLTCIVSLV GLTGNAVVLW
60 70 80 90 100
LLGCRMRRNA FSIYILNLAA ADFLFLSGRL IYSLLSFISI PHTISKILYP
110 120 130 140 150
VMMFSYFAGL SFLSAVSTER CLSVLWPIWY RCHRPTHLSA VVCVLLWALS
160 170 180 190 200
LLRSILEWML CGFLFSGADS AWCQTSDFIT VAWLIFLCVV LCGSSLVLLI
210 220 230 240 250
RILCGSRKIP LTRLYVTILL TVLVFLLCGL PFGIQFFLFL WIHVDREVLF
260 270 280 290 300
CHVHLVSIFL SALNSSANPI IYFFVGSFRQ RQNRQNLKLV LQRALQDASE
310 320
VDEGGGQLPE EILELSGSRL EQ
Length:322
Mass (Da):36,250
Last modified:December 1, 2001 - v1
Checksum:iC7F3A9F4418E8AD1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51I → V in AAL86880 (PubMed:11850634).Curated
Sequence conflicti278 – 2781F → L in AAL86880 (PubMed:11850634).Curated
Sequence conflicti298 – 2992AS → TP in AAL86880 (PubMed:11850634).Curated
Sequence conflicti307 – 3071Q → W in AAL86880 (PubMed:11850634).Curated
Sequence conflicti310 – 3123EEI → QET in AAL86880 (PubMed:11850634).Curated
Sequence conflicti321 – 3211E → D in AAH96835 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti36 – 361I → V.
Corresponds to variant rs11024885 [ dbSNP | Ensembl ].
VAR_019432

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY042213 Genomic DNA. Translation: AAK91804.1.
AF474989 Genomic DNA. Translation: AAL86880.2.
AF474990 Genomic DNA. Translation: AAL86881.1.
AB065846 Genomic DNA. Translation: BAC06064.1.
AB083628 Genomic DNA. Translation: BAB89341.1.
AC023078 Genomic DNA. No translation available.
BC096835 mRNA. Translation: AAH96835.1.
CCDSiCCDS7846.1.
RefSeqiNP_671732.3. NM_147199.3.
UniGeneiHs.711459.

Genome annotation databases

EnsembliENST00000302797; ENSP00000305766; ENSG00000170255.
GeneIDi259249.
KEGGihsa:259249.
UCSCiuc001mpg.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY042213 Genomic DNA. Translation: AAK91804.1.
AF474989 Genomic DNA. Translation: AAL86880.2.
AF474990 Genomic DNA. Translation: AAL86881.1.
AB065846 Genomic DNA. Translation: BAC06064.1.
AB083628 Genomic DNA. Translation: BAB89341.1.
AC023078 Genomic DNA. No translation available.
BC096835 mRNA. Translation: AAH96835.1.
CCDSiCCDS7846.1.
RefSeqiNP_671732.3. NM_147199.3.
UniGeneiHs.711459.

3D structure databases

ProteinModelPortaliQ96LB2.
SMRiQ96LB2. Positions 28-285.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi129233. 4 interactions.
STRINGi9606.ENSP00000305766.

Chemistry

BindingDBiQ96LB2.
ChEMBLiCHEMBL5850.
GuidetoPHARMACOLOGYi156.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ96LB2.

Polymorphism and mutation databases

BioMutaiMRGPRX1.
DMDMi50401128.

Proteomic databases

PRIDEiQ96LB2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302797; ENSP00000305766; ENSG00000170255.
GeneIDi259249.
KEGGihsa:259249.
UCSCiuc001mpg.3. human.

Organism-specific databases

CTDi259249.
GeneCardsiGC11M018955.
HGNCiHGNC:17962. MRGPRX1.
MIMi607227. gene.
neXtProtiNX_Q96LB2.
PharmGKBiPA142671334.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG146549.
GeneTreeiENSGT00550000074531.
HOGENOMiHOG000015412.
HOVERGENiHBG003592.
InParanoidiQ96LB2.
KOiK08396.
OMAiSAWCQTS.
OrthoDBiEOG75MVX4.
PhylomeDBiQ96LB2.
TreeFamiTF336336.

Miscellaneous databases

GeneWikiiMRGPRX1.
GenomeRNAii259249.
NextBioi93086.
PROiQ96LB2.
SOURCEiSearch...

Gene expression databases

BgeeiQ96LB2.
CleanExiHS_MRGPRX1.
ExpressionAtlasiQ96LB2. baseline.
GenevisibleiQ96LB2. HS.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR026234. MRGPCRFAMILY.
[Graphical view]
PANTHERiPTHR11334. PTHR11334. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR02108. MRGPCRFAMILY.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A diverse family of GPCRs expressed in specific subsets of nociceptive sensory neurons."
    Dong X., Han S.-K., Zylka M.J., Simon M.I., Anderson D.J.
    Cell 106:619-632(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY.
  3. "Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
    Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Identification of G protein-coupled receptor genes from the human genome sequence."
    Takeda S., Kadowaki S., Haga T., Takaesu H., Mitaku S.
    FEBS Lett. 520:97-101(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Sensory neuron-specific GPCR Mrgprs are itch receptors mediating chloroquine-induced pruritus."
    Liu Q., Tang Z., Surdenikova L., Kim S., Patel K.N., Kim A., Ru F., Guan Y., Weng H.J., Geng Y., Undem B.J., Kollarik M., Chen Z.F., Anderson D.J., Dong X.
    Cell 139:1353-1365(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS ITCH RECEPTOR.

Entry informationi

Entry nameiMRGX1_HUMAN
AccessioniPrimary (citable) accession number: Q96LB2
Secondary accession number(s): Q4V9L2, Q8TDD8, Q8TDD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 1, 2001
Last modified: July 22, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Activation of this receptor requires concentrations that exceed the chloroquine concentrations observed in plasma of patients undergoing chloroquine treatment. However, chloroquine accumulates at much higher level in the skin where the receptor is located. Chloroquine-induced itch is very common among black Africans (up to 70%) but less common in other populations.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.