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Q96LB2

- MRGX1_HUMAN

UniProt

Q96LB2 - MRGX1_HUMAN

Protein

Mas-related G-protein coupled receptor member X1

Gene

MRGPRX1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells. G(alpha)q proteins are involved in the calcium-signaling pathway. Activated by the antimalarial drug, chloroquine. May mediate chloroquine-induced itch, in a histamine-independent manner.2 Publications

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: UniProtKB-KW

    GO - Biological processi

    1. acute-phase response Source: UniProtKB-KW

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Acute phase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mas-related G-protein coupled receptor member X1
    Alternative name(s):
    Sensory neuron-specific G-protein coupled receptor 3/4
    Gene namesi
    Name:MRGPRX1
    Synonyms:MRGX1, SNSR3, SNSR4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:17962. MRGPRX1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142671334.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 322322Mas-related G-protein coupled receptor member X1PRO_0000069772Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi16 – 161N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ96LB2.

    PTM databases

    PhosphoSiteiQ96LB2.

    Expressioni

    Tissue specificityi

    Uniquely localized in a subset of small dorsal root and trigeminal sensory neurons.1 Publication

    Gene expression databases

    BgeeiQ96LB2.
    CleanExiHS_MRGPRX1.
    GenevestigatoriQ96LB2.

    Interactioni

    Protein-protein interaction databases

    BioGridi129233. 4 interactions.
    STRINGi9606.ENSP00000305766.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96LB2.
    SMRiQ96LB2. Positions 28-285.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3131ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini53 – 6715CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini89 – 968ExtracellularSequence Analysis
    Topological domaini118 – 14427CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini166 – 17712ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini199 – 22123CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini243 – 25412ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini276 – 32247CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei32 – 5221Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei68 – 8821Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei97 – 11721Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei145 – 16521Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei178 – 19821Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei222 – 24221Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei255 – 27521Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Mas subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG146549.
    HOGENOMiHOG000015412.
    HOVERGENiHBG003592.
    KOiK08396.
    OMAiSAWCQTS.
    OrthoDBiEOG75MVX4.
    PhylomeDBiQ96LB2.
    TreeFamiTF336336.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR026234. MRGPCRFAMILY.
    [Graphical view]
    PANTHERiPTHR11334. PTHR11334. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR02108. MRGPCRFAMILY.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q96LB2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPTISTLDT ELTPINGTEE TLCYKQTLSL TVLTCIVSLV GLTGNAVVLW    50
    LLGCRMRRNA FSIYILNLAA ADFLFLSGRL IYSLLSFISI PHTISKILYP 100
    VMMFSYFAGL SFLSAVSTER CLSVLWPIWY RCHRPTHLSA VVCVLLWALS 150
    LLRSILEWML CGFLFSGADS AWCQTSDFIT VAWLIFLCVV LCGSSLVLLI 200
    RILCGSRKIP LTRLYVTILL TVLVFLLCGL PFGIQFFLFL WIHVDREVLF 250
    CHVHLVSIFL SALNSSANPI IYFFVGSFRQ RQNRQNLKLV LQRALQDASE 300
    VDEGGGQLPE EILELSGSRL EQ 322
    Length:322
    Mass (Da):36,250
    Last modified:December 1, 2001 - v1
    Checksum:iC7F3A9F4418E8AD1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51I → V in AAL86880. (PubMed:11850634)Curated
    Sequence conflicti278 – 2781F → L in AAL86880. (PubMed:11850634)Curated
    Sequence conflicti298 – 2992AS → TP in AAL86880. (PubMed:11850634)Curated
    Sequence conflicti307 – 3071Q → W in AAL86880. (PubMed:11850634)Curated
    Sequence conflicti310 – 3123EEI → QET in AAL86880. (PubMed:11850634)Curated
    Sequence conflicti321 – 3211E → D in AAH96835. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti36 – 361I → V.
    Corresponds to variant rs11024885 [ dbSNP | Ensembl ].
    VAR_019432

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY042213 Genomic DNA. Translation: AAK91804.1.
    AF474989 Genomic DNA. Translation: AAL86880.2.
    AF474990 Genomic DNA. Translation: AAL86881.1.
    AB065846 Genomic DNA. Translation: BAC06064.1.
    AB083628 Genomic DNA. Translation: BAB89341.1.
    AC023078 Genomic DNA. No translation available.
    BC096835 mRNA. Translation: AAH96835.1.
    CCDSiCCDS7846.1.
    RefSeqiNP_671732.3. NM_147199.3.
    UniGeneiHs.711459.

    Genome annotation databases

    EnsembliENST00000302797; ENSP00000305766; ENSG00000170255.
    GeneIDi259249.
    KEGGihsa:259249.
    UCSCiuc001mpg.3. human.

    Polymorphism databases

    DMDMi50401128.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY042213 Genomic DNA. Translation: AAK91804.1 .
    AF474989 Genomic DNA. Translation: AAL86880.2 .
    AF474990 Genomic DNA. Translation: AAL86881.1 .
    AB065846 Genomic DNA. Translation: BAC06064.1 .
    AB083628 Genomic DNA. Translation: BAB89341.1 .
    AC023078 Genomic DNA. No translation available.
    BC096835 mRNA. Translation: AAH96835.1 .
    CCDSi CCDS7846.1.
    RefSeqi NP_671732.3. NM_147199.3.
    UniGenei Hs.711459.

    3D structure databases

    ProteinModelPortali Q96LB2.
    SMRi Q96LB2. Positions 28-285.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 129233. 4 interactions.
    STRINGi 9606.ENSP00000305766.

    Chemistry

    BindingDBi Q96LB2.
    ChEMBLi CHEMBL5850.
    GuidetoPHARMACOLOGYi 156.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei Q96LB2.

    Polymorphism databases

    DMDMi 50401128.

    Proteomic databases

    PRIDEi Q96LB2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000302797 ; ENSP00000305766 ; ENSG00000170255 .
    GeneIDi 259249.
    KEGGi hsa:259249.
    UCSCi uc001mpg.3. human.

    Organism-specific databases

    CTDi 259249.
    GeneCardsi GC11M018955.
    HGNCi HGNC:17962. MRGPRX1.
    MIMi 607227. gene.
    neXtProti NX_Q96LB2.
    PharmGKBi PA142671334.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG146549.
    HOGENOMi HOG000015412.
    HOVERGENi HBG003592.
    KOi K08396.
    OMAi SAWCQTS.
    OrthoDBi EOG75MVX4.
    PhylomeDBi Q96LB2.
    TreeFami TF336336.

    Miscellaneous databases

    GeneWikii MRGPRX1.
    GenomeRNAii 259249.
    NextBioi 93086.
    PROi Q96LB2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q96LB2.
    CleanExi HS_MRGPRX1.
    Genevestigatori Q96LB2.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR026234. MRGPCRFAMILY.
    [Graphical view ]
    PANTHERi PTHR11334. PTHR11334. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR02108. MRGPCRFAMILY.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A diverse family of GPCRs expressed in specific subsets of nociceptive sensory neurons."
      Dong X., Han S.-K., Zylka M.J., Simon M.I., Anderson D.J.
      Cell 106:619-632(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY.
    3. "Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
      Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y.
      Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Identification of G protein-coupled receptor genes from the human genome sequence."
      Takeda S., Kadowaki S., Haga T., Takaesu H., Mitaku S.
      FEBS Lett. 520:97-101(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. "Sensory neuron-specific GPCR Mrgprs are itch receptors mediating chloroquine-induced pruritus."
      Liu Q., Tang Z., Surdenikova L., Kim S., Patel K.N., Kim A., Ru F., Guan Y., Weng H.J., Geng Y., Undem B.J., Kollarik M., Chen Z.F., Anderson D.J., Dong X.
      Cell 139:1353-1365(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS ITCH RECEPTOR.

    Entry informationi

    Entry nameiMRGX1_HUMAN
    AccessioniPrimary (citable) accession number: Q96LB2
    Secondary accession number(s): Q4V9L2, Q8TDD8, Q8TDD9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Activation of this receptor requires concentrations that exceed the chloroquine concentrations observed in plasma of patients undergoing chloroquine treatment. However, chloroquine accumulates at much higher level in the skin where the receptor is located. Chloroquine-induced itch is very common among black Africans (up to 70%) but less common in other populations.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3