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Protein

Mas-related G-protein coupled receptor member X1

Gene

MRGPRX1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22)(PubMed:26582731). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells. G(alpha)q proteins are involved in the calcium-signaling pathway. Activated by the antimalarial drug, chloroquine. May mediate chloroquine-induced itch, in a histamine-independent manner.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Acute phase

Enzyme and pathway databases

BioCyciZFISH:ENSG00000170255-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mas-related G-protein coupled receptor member X1
Alternative name(s):
Sensory neuron-specific G-protein coupled receptor 3/4
Gene namesi
Name:MRGPRX1
Synonyms:MRGX1, SNSR3, SNSR4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:17962. MRGPRX1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 31ExtracellularSequence analysisAdd BLAST31
Transmembranei32 – 52Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini53 – 67CytoplasmicSequence analysisAdd BLAST15
Transmembranei68 – 88Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini89 – 96ExtracellularSequence analysis8
Transmembranei97 – 117Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini118 – 144CytoplasmicSequence analysisAdd BLAST27
Transmembranei145 – 165Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini166 – 177ExtracellularSequence analysisAdd BLAST12
Transmembranei178 – 198Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini199 – 221CytoplasmicSequence analysisAdd BLAST23
Transmembranei222 – 242Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini243 – 254ExtracellularSequence analysisAdd BLAST12
Transmembranei255 – 275Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini276 – 322CytoplasmicSequence analysisAdd BLAST47

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi259249.
OpenTargetsiENSG00000170255.
PharmGKBiPA142671334.

Chemistry databases

ChEMBLiCHEMBL5850.
GuidetoPHARMACOLOGYi156.

Polymorphism and mutation databases

BioMutaiMRGPRX1.
DMDMi50401128.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000697721 – 322Mas-related G-protein coupled receptor member X1Add BLAST322

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi16N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ96LB2.
PeptideAtlasiQ96LB2.
PRIDEiQ96LB2.

PTM databases

iPTMnetiQ96LB2.
PhosphoSitePlusiQ96LB2.

Expressioni

Tissue specificityi

Uniquely localized in a subset of small dorsal root and trigeminal sensory neurons.1 Publication

Gene expression databases

BgeeiENSG00000170255.
CleanExiHS_MRGPRX1.
ExpressionAtlasiQ96LB2. baseline and differential.
GenevisibleiQ96LB2. HS.

Interactioni

Protein-protein interaction databases

BioGridi129233. 4 interactors.
STRINGi9606.ENSP00000305766.

Chemistry databases

BindingDBiQ96LB2.

Structurei

3D structure databases

ProteinModelPortaliQ96LB2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Mas subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J9NM. Eukaryota.
ENOG4111AUB. LUCA.
GeneTreeiENSGT00550000074531.
HOGENOMiHOG000015412.
HOVERGENiHBG003592.
InParanoidiQ96LB2.
KOiK08396.
OMAiSAWCQTS.
OrthoDBiEOG091G0CSX.
PhylomeDBiQ96LB2.
TreeFamiTF336336.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR026234. MRGPCRFAMILY.
IPR027338. MRGPCRX1.
[Graphical view]
PANTHERiPTHR11334. PTHR11334. 1 hit.
PTHR11334:SF22. PTHR11334:SF22. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR02108. MRGPCRFAMILY.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96LB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPTISTLDT ELTPINGTEE TLCYKQTLSL TVLTCIVSLV GLTGNAVVLW
60 70 80 90 100
LLGCRMRRNA FSIYILNLAA ADFLFLSGRL IYSLLSFISI PHTISKILYP
110 120 130 140 150
VMMFSYFAGL SFLSAVSTER CLSVLWPIWY RCHRPTHLSA VVCVLLWALS
160 170 180 190 200
LLRSILEWML CGFLFSGADS AWCQTSDFIT VAWLIFLCVV LCGSSLVLLI
210 220 230 240 250
RILCGSRKIP LTRLYVTILL TVLVFLLCGL PFGIQFFLFL WIHVDREVLF
260 270 280 290 300
CHVHLVSIFL SALNSSANPI IYFFVGSFRQ RQNRQNLKLV LQRALQDASE
310 320
VDEGGGQLPE EILELSGSRL EQ
Length:322
Mass (Da):36,250
Last modified:December 1, 2001 - v1
Checksum:iC7F3A9F4418E8AD1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5I → V in AAL86880 (PubMed:11850634).Curated1
Sequence conflicti278F → L in AAL86880 (PubMed:11850634).Curated1
Sequence conflicti298 – 299AS → TP in AAL86880 (PubMed:11850634).Curated2
Sequence conflicti307Q → W in AAL86880 (PubMed:11850634).Curated1
Sequence conflicti310 – 312EEI → QET in AAL86880 (PubMed:11850634).Curated3
Sequence conflicti321E → D in AAH96835 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01943236I → V Polymorphism; no alteration in ligand-mediated receptor activity. 1 PublicationCorresponds to variant rs11024885dbSNPEnsembl.1
Natural variantiVAR_07546246A → T Polymorphism; no alteration in ligand-mediated receptor activity. 1 PublicationCorresponds to variant rs78179510dbSNPEnsembl.1
Natural variantiVAR_07546355R → L Polymorphism; no alteration in ligand-mediated receptor activity. 1 PublicationCorresponds to variant rs55954376dbSNPEnsembl.1
Natural variantiVAR_075464131R → S Polymorphism; decrease in ligand-mediated and ligand-independent receptor activity. 1 PublicationCorresponds to variant rs111448117dbSNPEnsembl.1
Natural variantiVAR_075465133H → R Polymorphism; increase in ligand-mediated receptor activity. 1 PublicationCorresponds to variant rs140351170dbSNPEnsembl.1
Natural variantiVAR_075466137H → R Polymorphism; no alteration in ligand-mediated receptor activity. 1 PublicationCorresponds to variant rs143702818dbSNPEnsembl.1
Natural variantiVAR_075467273F → L Polymorphism; no alteration in ligand-mediated receptor activity. 1 PublicationCorresponds to variant rs137866403dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY042213 Genomic DNA. Translation: AAK91804.1.
AF474989 Genomic DNA. Translation: AAL86880.2.
AF474990 Genomic DNA. Translation: AAL86881.1.
AB065846 Genomic DNA. Translation: BAC06064.1.
AB083628 Genomic DNA. Translation: BAB89341.1.
AC023078 Genomic DNA. No translation available.
BC096835 mRNA. Translation: AAH96835.1.
CCDSiCCDS7846.1.
RefSeqiNP_671732.3. NM_147199.3.
UniGeneiHs.711459.

Genome annotation databases

EnsembliENST00000302797; ENSP00000305766; ENSG00000170255.
GeneIDi259249.
KEGGihsa:259249.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY042213 Genomic DNA. Translation: AAK91804.1.
AF474989 Genomic DNA. Translation: AAL86880.2.
AF474990 Genomic DNA. Translation: AAL86881.1.
AB065846 Genomic DNA. Translation: BAC06064.1.
AB083628 Genomic DNA. Translation: BAB89341.1.
AC023078 Genomic DNA. No translation available.
BC096835 mRNA. Translation: AAH96835.1.
CCDSiCCDS7846.1.
RefSeqiNP_671732.3. NM_147199.3.
UniGeneiHs.711459.

3D structure databases

ProteinModelPortaliQ96LB2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi129233. 4 interactors.
STRINGi9606.ENSP00000305766.

Chemistry databases

BindingDBiQ96LB2.
ChEMBLiCHEMBL5850.
GuidetoPHARMACOLOGYi156.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ96LB2.
PhosphoSitePlusiQ96LB2.

Polymorphism and mutation databases

BioMutaiMRGPRX1.
DMDMi50401128.

Proteomic databases

PaxDbiQ96LB2.
PeptideAtlasiQ96LB2.
PRIDEiQ96LB2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302797; ENSP00000305766; ENSG00000170255.
GeneIDi259249.
KEGGihsa:259249.

Organism-specific databases

CTDi259249.
DisGeNETi259249.
GeneCardsiMRGPRX1.
HGNCiHGNC:17962. MRGPRX1.
MIMi607227. gene.
neXtProtiNX_Q96LB2.
OpenTargetsiENSG00000170255.
PharmGKBiPA142671334.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J9NM. Eukaryota.
ENOG4111AUB. LUCA.
GeneTreeiENSGT00550000074531.
HOGENOMiHOG000015412.
HOVERGENiHBG003592.
InParanoidiQ96LB2.
KOiK08396.
OMAiSAWCQTS.
OrthoDBiEOG091G0CSX.
PhylomeDBiQ96LB2.
TreeFamiTF336336.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000170255-MONOMER.

Miscellaneous databases

GeneWikiiMRGPRX1.
GenomeRNAii259249.
PROiQ96LB2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170255.
CleanExiHS_MRGPRX1.
ExpressionAtlasiQ96LB2. baseline and differential.
GenevisibleiQ96LB2. HS.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR026234. MRGPCRFAMILY.
IPR027338. MRGPCRX1.
[Graphical view]
PANTHERiPTHR11334. PTHR11334. 1 hit.
PTHR11334:SF22. PTHR11334:SF22. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR02108. MRGPCRFAMILY.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMRGX1_HUMAN
AccessioniPrimary (citable) accession number: Q96LB2
Secondary accession number(s): Q4V9L2, Q8TDD8, Q8TDD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Activation of this receptor requires concentrations that exceed the chloroquine concentrations observed in plasma of patients undergoing chloroquine treatment. However, chloroquine accumulates at much higher level in the skin where the receptor is located. Chloroquine-induced itch is very common among black Africans (up to 70%) but less common in other populations.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.