Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q96LB2 (MRGX1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mas-related G-protein coupled receptor member X1
Alternative name(s):
Sensory neuron-specific G-protein coupled receptor 3/4
Gene names
Name:MRGPRX1
Synonyms:MRGX1, SNSR3, SNSR4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length322 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells. G(alpha)q proteins are involved in the calcium-signaling pathway. Activated by the antimalarial drug, chloroquine. May mediate chloroquine-induced itch, in a histamine-independent manner. Ref.2 Ref.7

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Uniquely localized in a subset of small dorsal root and trigeminal sensory neurons. Ref.2

Miscellaneous

Activation of this receptor requires concentrations that exceed the chloroquine concentrations observed in plasma of patients undergoing chloroquine treatment. However, chloroquine accumulates at much higher level in the skin where the receptor is located. Chloroquine-induced itch is very common among black Africans (up to 70%) but less common in other populations.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Mas subfamily.

Ontologies

Keywords
   Biological processAcute phase
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processacute-phase response

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionG-protein coupled receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 322322Mas-related G-protein coupled receptor member X1
PRO_0000069772

Regions

Topological domain1 – 3131Extracellular Potential
Transmembrane32 – 5221Helical; Name=1; Potential
Topological domain53 – 6715Cytoplasmic Potential
Transmembrane68 – 8821Helical; Name=2; Potential
Topological domain89 – 968Extracellular Potential
Transmembrane97 – 11721Helical; Name=3; Potential
Topological domain118 – 14427Cytoplasmic Potential
Transmembrane145 – 16521Helical; Name=4; Potential
Topological domain166 – 17712Extracellular Potential
Transmembrane178 – 19821Helical; Name=5; Potential
Topological domain199 – 22123Cytoplasmic Potential
Transmembrane222 – 24221Helical; Name=6; Potential
Topological domain243 – 25412Extracellular Potential
Transmembrane255 – 27521Helical; Name=7; Potential
Topological domain276 – 32247Cytoplasmic Potential

Amino acid modifications

Glycosylation161N-linked (GlcNAc...) Potential

Natural variations

Natural variant361I → V.
Corresponds to variant rs11024885 [ dbSNP | Ensembl ].
VAR_019432

Experimental info

Sequence conflict51I → V in AAL86880. Ref.2
Sequence conflict2781F → L in AAL86880. Ref.2
Sequence conflict298 – 2992AS → TP in AAL86880. Ref.2
Sequence conflict3071Q → W in AAL86880. Ref.2
Sequence conflict310 – 3123EEI → QET in AAL86880. Ref.2
Sequence conflict3211E → D in AAH96835. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q96LB2 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: C7F3A9F4418E8AD1

FASTA32236,250
        10         20         30         40         50         60 
MDPTISTLDT ELTPINGTEE TLCYKQTLSL TVLTCIVSLV GLTGNAVVLW LLGCRMRRNA 

        70         80         90        100        110        120 
FSIYILNLAA ADFLFLSGRL IYSLLSFISI PHTISKILYP VMMFSYFAGL SFLSAVSTER 

       130        140        150        160        170        180 
CLSVLWPIWY RCHRPTHLSA VVCVLLWALS LLRSILEWML CGFLFSGADS AWCQTSDFIT 

       190        200        210        220        230        240 
VAWLIFLCVV LCGSSLVLLI RILCGSRKIP LTRLYVTILL TVLVFLLCGL PFGIQFFLFL 

       250        260        270        280        290        300 
WIHVDREVLF CHVHLVSIFL SALNSSANPI IYFFVGSFRQ RQNRQNLKLV LQRALQDASE 

       310        320 
VDEGGGQLPE EILELSGSRL EQ 

« Hide

References

« Hide 'large scale' references
[1]"A diverse family of GPCRs expressed in specific subsets of nociceptive sensory neurons."
Dong X., Han S.-K., Zylka M.J., Simon M.I., Anderson D.J.
Cell 106:619-632(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Proenkephalin A gene products activate a new family of sensory neuron-specific GPCRs."
Lembo P.M.C., Grazzini E., Groblewski T., O'Donnell D., Roy M.-O., Zhang J., Hoffert C., Cao J., Schmidt R., Pelletier M., Labarre M., Gosselin M., Fortin Y., Banville D., Shen S., Stroem P., Payza K., Dray A., Walker P., Ahmad S.
Nat. Neurosci. 5:201-209(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY.
[3]"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Identification of G protein-coupled receptor genes from the human genome sequence."
Takeda S., Kadowaki S., Haga T., Takaesu H., Mitaku S.
FEBS Lett. 520:97-101(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"Sensory neuron-specific GPCR Mrgprs are itch receptors mediating chloroquine-induced pruritus."
Liu Q., Tang Z., Surdenikova L., Kim S., Patel K.N., Kim A., Ru F., Guan Y., Weng H.J., Geng Y., Undem B.J., Kollarik M., Chen Z.F., Anderson D.J., Dong X.
Cell 139:1353-1365(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS ITCH RECEPTOR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY042213 Genomic DNA. Translation: AAK91804.1.
AF474989 Genomic DNA. Translation: AAL86880.2.
AF474990 Genomic DNA. Translation: AAL86881.1.
AB065846 Genomic DNA. Translation: BAC06064.1.
AB083628 Genomic DNA. Translation: BAB89341.1.
AC023078 Genomic DNA. No translation available.
BC096835 mRNA. Translation: AAH96835.1.
CCDSCCDS7846.1.
RefSeqNP_671732.3. NM_147199.3.
UniGeneHs.711459.

3D structure databases

ProteinModelPortalQ96LB2.
SMRQ96LB2. Positions 28-285.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid129233. 4 interactions.
STRING9606.ENSP00000305766.

Chemistry

BindingDBQ96LB2.
ChEMBLCHEMBL5850.
GuidetoPHARMACOLOGY156.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ96LB2.

Polymorphism databases

DMDM50401128.

Proteomic databases

PRIDEQ96LB2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000302797; ENSP00000305766; ENSG00000170255.
GeneID259249.
KEGGhsa:259249.
UCSCuc001mpg.3. human.

Organism-specific databases

CTD259249.
GeneCardsGC11M018955.
HGNCHGNC:17962. MRGPRX1.
MIM607227. gene.
neXtProtNX_Q96LB2.
PharmGKBPA142671334.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG146549.
HOGENOMHOG000015412.
HOVERGENHBG003592.
KOK08396.
OMASAWCQTS.
OrthoDBEOG75MVX4.
PhylomeDBQ96LB2.
TreeFamTF336336.

Gene expression databases

BgeeQ96LB2.
CleanExHS_MRGPRX1.
GenevestigatorQ96LB2.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR026234. MRGPCRFAMILY.
[Graphical view]
PANTHERPTHR11334. PTHR11334. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR02108. MRGPCRFAMILY.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMRGPRX1.
GenomeRNAi259249.
NextBio93086.
PROQ96LB2.
SOURCESearch...

Entry information

Entry nameMRGX1_HUMAN
AccessionPrimary (citable) accession number: Q96LB2
Secondary accession number(s): Q4V9L2, Q8TDD8, Q8TDD9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries