Q96LA5 (FCRL2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fc receptor-like protein 2
Short name=FcR-like protein 2
Short name=FcRL2

Alternative name(s):
Fc receptor homolog 2
Short name=FcRH2
IFGP family protein 4
Immunoglobulin receptor translocation-associated protein 4
SH2 domain-containing phosphatase anchor protein 1
CD_antigen=CD307b

Gene names

Name:	FCRL2
Synonyms:	FCRH2, IFGP4, IRTA4, SPAP1
ORF Names:	UNQ9236/PRO31998

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May have an regulatory role in normal and neoplastic B cell development. Ref.1
Subunit structure	The tyrosine-phosphorylated isoform 2 interacts with PTPN6. Ref.2
Subcellular location	Cell membrane; Single-pass type I membrane protein Ref.11.
Tissue specificity	Expressed in the secondary lymphoid organs, spleen and lymph node. Expression is limited to the mature B-cell lines. Highly expressed in CD19 and within the mantle zones of the tonsil tissue. Isoform 2 is expressed in the spleen, peripheral blood and bone marrow. Isoform 2 and isoform 4 are expressed in B-cell lines. Preferentially expressed in memory B-cells (at protein level). Ref.1 Ref.2 Ref.3 Ref.11
Domain	Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). The phosphorylated ITIM motif bind the SH2 domain of PTPN6.
Post-translational modification	Isoform 2 is N- and O-glycosylated, and phosphorylated. Ref.2
Sequence similarities	Contains 4 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell signaling Non-traceable author statement Ref.2. Source: UniProtKB
Cellular_component	integral to membrane Non-traceable author statement Ref.2. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96LA5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96LA5-2)

Also known as: SPAP1A;

The sequence of this isoform differs from the canonical sequence as follows:
     1-253: Missing.
     254-295: RSLSAELEIP...SKVVNIPVRI → MWEWKICNSH...FLIAPLCLSV

Isoform 3 (identifier: Q96LA5-3)

Also known as: SPAP1B;

The sequence of this isoform differs from the canonical sequence as follows:
     1-253: Missing.
     254-295: RSLSAELEIP...SKVVNIPVRI → MWEWKICNSH...FLIAPLCLSV
     428-445: ESSATNEPRGASRPNPQE → CIYHLDSPYFAMTFPLLI
     446-508: Missing.

Isoform 4 (identifier: Q96LA5-4)

Also known as: SPAP1C;

The sequence of this isoform differs from the canonical sequence as follows:
     1-253: Missing.
     254-295: RSLSAELEIP...SKVVNIPVRI → MWEWKICNSH...FLIAPLCLSV
     389-397: PDGYRRDLM → GWVLPGYRV
     398-508: Missing.

Isoform 5 (identifier: Q96LA5-5)

The sequence of this isoform differs from the canonical sequence as follows:
     104-387: Missing.
     465-486: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.10
	Chain	20 – 508	489	Fc receptor-like protein 2		PRO_0000014761
Regions
	Topological domain	20 – 401	382	Extracellular Potential
	Transmembrane	402 – 422	21	Helical; Potential
	Topological domain	423 – 508	86	Cytoplasmic Potential
	Domain	20 – 98	79	Ig-like C2-type 1
	Domain	109 – 187	79	Ig-like C2-type 2
	Domain	201 – 290	90	Ig-like C2-type 3
	Domain	300 – 387	88	Ig-like C2-type 4
	Motif	446 – 451	6	ITIM motif 1
	Motif	460 – 465	6	ITIM motif 2
	Motif	472 – 477	6	ITIM motif 3
	Motif	500 – 505	6	ITIM motif 4
Amino acid modifications
	Glycosylation	204	1	N-linked (GlcNAc...) Potential
	Glycosylation	234	1	N-linked (GlcNAc...) Potential
	Glycosylation	343	1	N-linked (GlcNAc...) Potential
	Glycosylation	355	1	N-linked (GlcNAc...) Potential
	Glycosylation	365	1	N-linked (GlcNAc...) Potential
	Disulfide bond	128 ↔ 177		By similarity
	Disulfide bond	226 ↔ 275		By similarity
	Disulfide bond	321 ↔ 368		By similarity
Natural variations
	Alternative sequence	1 – 253	253	Missing in isoform 2, isoform 3 and isoform 4.		VSP_014111
	Alternative sequence	104 – 387	284	Missing in isoform 5.		VSP_014113
	Alternative sequence	254 – 295	42	RSLSA…IPVRI → MWEWKICNSHGARPFAEPAG WEFVNLLRHHKSFLIAPLCL SV in isoform 2, isoform 3 and isoform 4.		VSP_014112
	Alternative sequence	389 – 397	9	PDGYRRDLM → GWVLPGYRV in isoform 4.		VSP_014114
	Alternative sequence	398 – 508	111	Missing in isoform 4.		VSP_014115
	Alternative sequence	428 – 445	18	ESSAT…PNPQE → CIYHLDSPYFAMTFPLLI in isoform 3.		VSP_014116
	Alternative sequence	446 – 508	63	Missing in isoform 3.		VSP_014117
	Alternative sequence	465 – 486	22	Missing in isoform 5.		VSP_014118
	Natural variant	202	1	I → N. Corresponds to variant rs16839100 [ dbSNP | Ensembl ].		VAR_049873

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 9AB30E0411B41EDC	FASTA	508	55,542
	10 20 30 40 50 60 MLLWSLLVIF DAVTEQADSL TLVAPSSVFE GDSIVLKCQG EQNWKIQKMA YHKDNKELSV 70 80 90 100 110 120 FKKFSDFLIQ SAVLSDSGNY FCSTKGQLFL WDKTSNIVKI KVQELFQRPV LTASSFQPIE 130 140 150 160 170 180 GGPVSLKCET RLSPQRLDVQ LQFCFFRENQ VLGSGWSSSP ELQISAVWSE DTGSYWCKAE 190 200 210 220 230 240 TVTHRIRKQS LQSQIHVQRI PISNVSLEIR APGGQVTEGQ KLILLCSVAG GTGNVTFSWY 250 260 270 280 290 300 REATGTSMGK KTQRSLSAEL EIPAVKESDA GKYYCRADNG HVPIQSKVVN IPVRIPVSRP 310 320 330 340 350 360 VLTLRSPGAQ AAVGDLLELH CEALRGSPPI LYQFYHEDVT LGNSSAPSGG GASFNLSLTA 370 380 390 400 410 420 EHSGNYSCEA NNGLGAQCSE AVPVSISGPD GYRRDLMTAG VLWGLFGVLG FTGVALLLYA 430 440 450 460 470 480 LFHKISGESS ATNEPRGASR PNPQEFTYSS PTPDMEELQP VYVNVGSVDV DVVYSQVWSM 490 500 QQPESSANIR TLLENKDSQV IYSSVKKS
	Isoform 2 (SPAP1A) [UniParc]. Checksum: 826E6447FB6BAB14	FASTA	255	27,546
	10 20 30 40 50 60 MWEWKICNSH GARPFAEPAG WEFVNLLRHH KSFLIAPLCL SVPVSRPVLT LRSPGAQAAV 70 80 90 100 110 120 GDLLELHCEA LRGSPPILYQ FYHEDVTLGN SSAPSGGGAS FNLSLTAEHS GNYSCEANNG 130 140 150 160 170 180 LGAQCSEAVP VSISGPDGYR RDLMTAGVLW GLFGVLGFTG VALLLYALFH KISGESSATN 190 200 210 220 230 240 EPRGASRPNP QEFTYSSPTP DMEELQPVYV NVGSVDVDVV YSQVWSMQQP ESSANIRTLL 250 ENKDSQVIYS SVKKS
	Isoform 3 (SPAP1B) [UniParc]. Checksum: C9962BBB4B15B08D	FASTA	192	20,680
	10 20 30 40 50 60 MWEWKICNSH GARPFAEPAG WEFVNLLRHH KSFLIAPLCL SVPVSRPVLT LRSPGAQAAV 70 80 90 100 110 120 GDLLELHCEA LRGSPPILYQ FYHEDVTLGN SSAPSGGGAS FNLSLTAEHS GNYSCEANNG 130 140 150 160 170 180 LGAQCSEAVP VSISGPDGYR RDLMTAGVLW GLFGVLGFTG VALLLYALFH KISGCIYHLD 190 SPYFAMTFPL LI
	Isoform 4 (SPAP1C) [UniParc]. Checksum: D03F859338237CA5	FASTA	144	15,373
	10 20 30 40 50 60 MWEWKICNSH GARPFAEPAG WEFVNLLRHH KSFLIAPLCL SVPVSRPVLT LRSPGAQAAV 70 80 90 100 110 120 GDLLELHCEA LRGSPPILYQ FYHEDVTLGN SSAPSGGGAS FNLSLTAEHS GNYSCEANNG 130 140 LGAQCSEAVP VSISGGWVLP GYRV
	Isoform 5 [UniParc]. Checksum: 9D6C578B6E136E6E	FASTA	202	22,533
	10 20 30 40 50 60 MLLWSLLVIF DAVTEQADSL TLVAPSSVFE GDSIVLKCQG EQNWKIQKMA YHKDNKELSV 70 80 90 100 110 120 FKKFSDFLIQ SAVLSDSGNY FCSTKGQLFL WDKTSNIVKI KVQGPDGYRR DLMTAGVLWG 130 140 150 160 170 180 LFGVLGFTGV ALLLYALFHK ISGESSATNE PRGASRPNPQ EFTYSSPTPD MEELQPVYVN 190 200 ANIRTLLENK DSQVIYSSVK KS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a family of Fc receptor homologs with preferential B cell expression." Davis R.S., Wang Y.-H., Kubagawa H., Cooper M.D. Proc. Natl. Acad. Sci. U.S.A. 98:9772-9777(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY. Tissue: Lymph node.
[2]	"Molecular cloning and characterization of SPAP1, an inhibitory receptor." Xu M.-J., Zhao R., Zhao Z.J. Biochem. Biophys. Res. Commun. 280:768-775(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4), INTERACTION WITH PTPN6, TISSUE SPECIFICITY, PHOSPHORYLATION, GLYCOSYLATION.
[3]	"IRTAs: a new family of immunoglobulin-like receptors differentially expressed in B cells." Miller I., Hatzivassiliou G., Cattoretti G., Mendelsohn C., Dalla-Favera R. Blood 99:2662-2669(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[4]	"A family of highly diverse human and mouse genes structurally links leukocyte FcR, gp42 and PECAM-1." Guselnikov S.V., Ershova S.A., Mechetina L.V., Najakshin A.M., Volkova O.Y., Alabyev B.Y., Taranin A.V. Immunogenetics 54:87-95(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Tonsil.
[5]	Livingston R.J., Shaffer T., McFarland I., Nguyen C.P., Stanaway I.B., Rajkumar N., Johnson E.J., da Ponte S.H., Willa H., Ahearn M.O., Bertucci C., Acklestad J., Carroll A., Swanson J., Gildersleeve H.I., Nickerson D.A. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 5).
[10]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-34.
[11]	"Expression pattern of the human FcRH/IRTA receptors in normal tissue and in B-chronic lymphocytic leukemia." Polson A.G., Zheng B., Elkins K., Chang W., Du C., Dowd P., Yen L., Tan C., Hongo J.-A., Koeppen H., Ebens A. Int. Immunol. 18:1363-1373(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY043465 mRNA. Translation: AAK91778.1. AF319438 mRNA. Translation: AAK01402.1. AF319439 mRNA. Translation: AAK01403.1. AF319440 mRNA. Translation: AAK01404.1. AF459633 mRNA. Translation: AAL60249.1. AF390037 mRNA. Translation: AAM12152.1. EF064733 Genomic DNA. Translation: ABK41916.1. AY358130 mRNA. Translation: AAQ88497.1. AL356276 Genomic DNA. Translation: CAH73060.1. AL356276 Genomic DNA. Translation: CAH73061.1. AL356276 Genomic DNA. Translation: CAH73062.1. AL356276 Genomic DNA. Translation: CAH73063.1. CH471121 Genomic DNA. Translation: EAW52864.1. CH471121 Genomic DNA. Translation: EAW52867.1. CH471121 Genomic DNA. Translation: EAW52869.1. BC069185 mRNA. Translation: AAH69185.1. BC129836 mRNA. Translation: AAI29837.1.
IPI	IPI00013114. IPI00097529. IPI00107694. IPI00382413. IPI00431992.
PIR	JC7593.
RefSeq	NP_110391.2. NM_030764.3.
UniGene	Hs.437393.
3D structure databases
ProteinModelPortal	Q96LA5.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000355157.
PTM databases
PhosphoSite	Q96LA5.
Polymorphism databases
DMDM	68052374.
Proteomic databases
PaxDb	Q96LA5.
PRIDE	Q96LA5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000361516; ENSP00000355157; ENSG00000132704. ENST00000368181; ENSP00000357163; ENSG00000132704. ENST00000469986; ENSP00000417393; ENSG00000132704.
GeneID	79368.
KEGG	hsa:79368.
UCSC	uc001frd.2. human. uc001fre.2. human. uc009wsp.2. human.
Organism-specific databases
CTD	79368.
GeneCards	GC01M157715.
HGNC	HGNC:14875. FCRL2.
HPA	CAB017619. HPA041426.
MIM	606509. gene.
neXtProt	NX_Q96LA5.
PharmGKB	PA37913.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG151212.
HOVERGEN	HBG074073.
InParanoid	Q96LA5.
OMA	QELFQHP.
OrthoDB	EOG4K9BBP.
Gene expression databases
ArrayExpress	Q96LA5.
Bgee	Q96LA5.
CleanEx	HS_FCRL2.
Genevestigator	Q96LA5.
GermOnline	ENSG00000132704. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.10. 4 hits.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR003598. Ig_sub2. [Graphical view]
SMART	SM00409. IG. 3 hits. SM00408. IGc2. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 3 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	79368.
NextBio	68341.
SOURCE	Search...

Entry information

Entry name

FCRL2_HUMAN

Accession

Primary (citable) accession number: Q96LA5
Secondary accession number(s): A0N0M5 , A1L307, A6NMS0, Q6NTA1, Q9BZI4, Q9BZI5, Q9BZI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.