Q96L96 (ALPK3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-protein kinase 3
EC=2.7.11.-

Alternative name(s):
Muscle alpha-protein kinase

Gene names

Name:	ALPK3
Synonyms:	KIAA1330, MAK

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1907 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Kinase that recognizes phosphorylation sites in which the surrounding peptides have an alpha-helical conformation. Plays a role in cardiomyocyte differentiation By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily. Contains 1 alpha-type protein kinase domain. Contains 2 Ig-like (immunoglobulin-like) domains.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Repeat
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	heart development Inferred from sequence or structural similarity. Source: HGNC
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: HGNC
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1907	1907	Alpha-protein kinase 3		PRO_0000260031
Regions
	Domain	279 – 370	92	Ig-like 1
	Domain	1476 – 1564	89	Ig-like 2
	Domain	1592 – 1827	236	Alpha-type protein kinase
	Compositional bias	170 – 175	6	Poly-Ala
	Compositional bias	517 – 521	5	Poly-Lys
	Compositional bias	766 – 771	6	Poly-Ser
	Compositional bias	1166 – 1172	7	Poly-Leu
Amino acid modifications
	Disulfide bond	1498 ↔ 1548		By similarity
Natural variations
	Natural variant	336	1	R → H. Ref.4 Corresponds to variant rs34407151 [ dbSNP | Ensembl ].		VAR_041524
	Natural variant	338	1	T → I. Ref.4		VAR_041525
	Natural variant	414	1	T → S. Ref.4 Corresponds to variant rs3803403 [ dbSNP | Ensembl ].		VAR_028989
	Natural variant	433	1	Q → E in a lung large cell carcinoma sample; somatic mutation. Ref.4		VAR_041526
	Natural variant	579	1	G → E. Ref.4 Corresponds to variant rs3803405 [ dbSNP | Ensembl ].		VAR_028990
	Natural variant	602	1	Q → R. Ref.4		VAR_041527
	Natural variant	663	1	G → D. Ref.4		VAR_041528
	Natural variant	761	1	T → M. Ref.4 Corresponds to variant rs16974569 [ dbSNP | Ensembl ].		VAR_028991
	Natural variant	836	1	R → L. Ref.4		VAR_041529
	Natural variant	929	1	E → D. Ref.4		VAR_041530
	Natural variant	1013	1	E → K. Corresponds to variant rs35633849 [ dbSNP | Ensembl ].		VAR_057743
	Natural variant	1137	1	A → G. Corresponds to variant rs34173528 [ dbSNP | Ensembl ].		VAR_057744
	Natural variant	1299	1	P → L. Ref.1 Ref.2 Ref.4 Corresponds to variant rs306197 [ dbSNP | Ensembl ].		VAR_028992
	Natural variant	1364	1	G → E in a metastatic melanoma sample; somatic mutation. Ref.4		VAR_041531
	Natural variant	1412	1	R → W. Ref.4		VAR_041532
	Natural variant	1557	1	A → D. Ref.4 Corresponds to variant rs34775428 [ dbSNP | Ensembl ].		VAR_041533
	Natural variant	1622	1	L → P. Ref.4 Corresponds to variant rs187316 [ dbSNP | Ensembl ].		VAR_028993
	Natural variant	1873	1	A → V. Corresponds to variant rs36002219 [ dbSNP | Ensembl ].		VAR_057745
Experimental info
	Sequence conflict	210	1	S → T in AAK95951. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q96L96 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: 3896A1EC187BF5C7	FASTA	1,907	201,272
	10 20 30 40 50 60 MEVAWLVYVL GQQPLARQGE GQSRLVPGRG LVLWLPGLPR SSPSWPAVDL APLAPARPRG 70 80 90 100 110 120 PLICHTGHEQ AGREPGPGSS TKGPVLHDQD TRCAFLPRPP GPLQTRRYCR HQGRQGSGLG 130 140 150 160 170 180 AGPGAGTWAP APPGVSKPRC PGRARPGEGQ QQVTTARPPA INRGARQPRA GAAAAGRGPG 190 200 210 220 230 240 AGAWRTGEAA ASAGPAVGEG GAMGSRRAPS RGWGAGGRSG AGGDGEDDGP VWIPSPASRS 250 260 270 280 290 300 YLLSVRPETS LSSNRLSHPS SGRSTFCSII AQLTEETQPL FETTLKSRSV SEDSDVRFTC 310 320 330 340 350 360 IVTGYPEPEV TWYKDDTELD RYCGLPKYEI THQGNRHTLQ LYRCREEDAA IYQASAQNSK 370 380 390 400 410 420 GIVSCSGVLE VGTMTEYKIH QRWFAKLKRK AAAKLREIEQ SWKHEKAVPG EVDTLRKLSP 430 440 450 460 470 480 DRFQRKRRLS GAQAPGPSVP TREPEGGTLA AWQEGETETA QHSGLGLINS FASGEVTTNG 490 500 510 520 530 540 EAAPENGEDG EHGLLTYICD AMELGPQRAL KEESGAKKKK KDEESKQGLR KPELEKAAQS 550 560 570 580 590 600 RRSSENCIPS SDEPDSCGTQ GPVGVEQVQT QPRGRAARGP GSSGTDSTRK PASAVGTPDK 610 620 630 640 650 660 AQKAPGPGPG QEVYFSLKDM YLENTQAVRP LGEEGPQTLS VRAPGESPKG KAPLRARSEG 670 680 690 700 710 720 VPGAPGQPTH SLTPQPTRPF NRKRFAPPKP KGEATTDSKP ISSLSQAPEC GAQSLGKAPP 730 740 750 760 770 780 QASVQVPTPP ARRRHGTRDS TLQGQAGHRT PGEVLECQTT TAPTMSASSS SDVASIGVST 790 800 810 820 830 840 SGSQGIIEPM DMETQEDGRT SANQRTGSKK NVQADGKIQV DGRTRGDGTQ TAQRTRADRK 850 860 870 880 890 900 TQVDAGTQES KRPQSDRSAQ KGMMTQGRAE TQLETTQAGE KIQEDRKAQA DKGTQEDRRM 910 920 930 940 950 960 QGEKGMQGEK GTQSEGSAPT AMEGQSEQEV ATSLGPPSRT PKLPPTAGPR APLNIECFVQ 970 980 990 1000 1010 1020 TPEGSCFPKK PGCLPRSEEA VVTASRNHEQ TVLGPLSGNL MLPAQPPHEG SVEQVGGERC 1030 1040 1050 1060 1070 1080 RGPQSSGPVE AKQEDSPFQC PKEERPGGVP CMDQGGCPLA GLSQEVPTMP SLPGTGLTAS 1090 1100 1110 1120 1130 1140 PKAGPCSTPT SQHGSTATFL PSEDQVLMSS APTLHLGLGT PTQSHPPETM ATSSEGACAQ 1150 1160 1170 1180 1190 1200 VPDVEGRTPG PRSCDPGLID SLKNYLLLLL KLSSTETSGA GGESQVGAAT GGLVPSATLT 1210 1220 1230 1240 1250 1260 PTVEVAGLSP RTSRRILERV ENNHLVQSAQ TLLLSPCTSR RLTGLLDREV QAGRQALAAA 1270 1280 1290 1300 1310 1320 RGSWGPGPSS LTVPAIVVDE EDPGLASEGA SEGEGEVSPE GPGLLGASQE SSMAGRLGEA 1330 1340 1350 1360 1370 1380 GGQAAPGQGP SAESIAQEPS QEEKFPGEAL TGLPAATPEE LALGARRKRF LPKVRAAGDG 1390 1400 1410 1420 1430 1440 EATTPEERES PTVSPRGPRK SLVPGSPGTP GRERRSPTQG RKASMLEVPR AEEELAAGDL 1450 1460 1470 1480 1490 1500 GPSPKAGGLD TEVALDEGKQ ETLAKPRKAK DLLKAPQVIR KIRVEQFPDA SGSLKLWCQF 1510 1520 1530 1540 1550 1560 FNILSDSVLT WAKDQRPVGE VGRSAGDEGP AALAIVQASP VDCGVYRCTI HNEHGSASTD 1570 1580 1590 1600 1610 1620 FCLSPEVLSG FISREEGEVG EEIEMTPMVF AKGLADSGCW GDKLFGRLVS EELRGGGYGC 1630 1640 1650 1660 1670 1680 GLRKASQAKV IYGLEPIFES GRTCIIKVSS LLVFGPSSET SLVGRNYDVT IQGCKIQNMS 1690 1700 1710 1720 1730 1740 REYCKIFAAE ARAAPGFGEV PEIIPLYLIY RPANNIPYAT LEEDLGKPLE SYCSREWGCA 1750 1760 1770 1780 1790 1800 EAPTASGSSE AMQKCQTFQH WLYQWTNGSF LVTDLAGVDW KMTDVQIATK LRGYQGLKES 1810 1820 1830 1840 1850 1860 CFPALLDRFA SSHQCNAYCE LLGLTPLKGP EAAHPQAKAK GSKSPSAGRK GSQLSPQPQK 1870 1880 1890 1900 KGLPSPQGTR KSAPSSKATP QASEPVTTQL LGQPPTQEEG SKAQGMR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Alpha-kinases: a new class of protein kinases with a novel catalytic domain." Ryazanov A.G., Pavur K.S., Dorovkov M.V. Curr. Biol. 9:R43-R45(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LEU-1299.
[2]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 963-1907, VARIANT LEU-1299. Tissue: Brain.
[3]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] HIS-336; ILE-338; SER-414; GLU-433; GLU-579; ARG-602; ASP-663; MET-761; LEU-836; ASP-929; LEU-1299; GLU-1364; TRP-1412; ASP-1557 AND PRO-1622.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY044449 mRNA. Translation: AAK95951.1. AB037751 mRNA. Translation: BAA92568.1. AC012291 Genomic DNA. No translation available.
IPI	IPI00064935.
RefSeq	NP_065829.3. NM_020778.4.
UniGene	Hs.459183.
3D structure databases
HSSP	HSSP built from PDB template 1TLK based on UniProtKB P56276.
ProteinModelPortal	Q96L96.
SMR	Q96L96. Positions 279-371, 1489-1564.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96L96. 1 interaction.
STRING	9606.ENSP00000258888.
PTM databases
PhosphoSite	Q96L96.
Polymorphism databases
DMDM	296434393.
Proteomic databases
PaxDb	Q96L96.
PRIDE	Q96L96.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000258888; ENSP00000258888; ENSG00000136383.
GeneID	57538.
KEGG	hsa:57538.
UCSC	uc002ble.3. human.
Organism-specific databases
CTD	57538.
GeneCards	GC15P085359.
H-InvDB	HIX0012537.
HGNC	HGNC:17574. ALPK3.
HPA	HPA026558.
neXtProt	NX_Q96L96.
PharmGKB	PA134921552.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG148552.
HOGENOM	HOG000169775.
HOVERGEN	HBG074162.
InParanoid	Q96L96.
KO	K08868.
OMA	KYEITHQ.
OrthoDB	EOG49GKG7.
PhylomeDB	Q96L96.
Gene expression databases
Bgee	Q96L96.
CleanEx	HS_ALPK3. HS_MAK.
Genevestigator	Q96L96.
GermOnline	ENSG00000136383. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.10. 2 hits.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR013098. Ig_I-set. IPR003598. Ig_sub2. IPR011009. Kinase-like_dom. IPR004166. MHCK_EF2_kinase. [Graphical view]
Pfam	PF02816. Alpha_kinase. 1 hit. PF07679. I-set. 1 hit. [Graphical view]
SMART	SM00811. Alpha_kinase. 1 hit. SM00408. IGc2. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51158. ALPHA_KINASE. 1 hit. PS50835. IG_LIKE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	57538.
NextBio	63964.

Entry information

Entry name

ALPK3_HUMAN

Accession

Primary (citable) accession number: Q96L96
Secondary accession number(s): Q9P2L6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.