Q96L93 (KI16B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinesin-like protein KIF16B

Alternative name(s):
Sorting nexin-23

Gene names

Name:	KIF16B
Synonyms:	C20orf23, KIAA1590, SNX23

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1317 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plus end-directed microtubule-dependent motor protein involved in endosome transport and receptor recycling and degradation. Regulates the plus end motility of early endosomes and the balance between recycling and degradation of receptors such as EGF receptor (EGFR) and FGF receptor (FGFR). Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. Ref.9
Subunit structure	Interacts with RAB14 By similarity. Interacts with PTPN21. Ref.11
Subcellular location	Cytoplasm › cytoskeleton Probable. Early endosome membrane. Note: It is unclear whether association with endosomes is mediated via phosphatidylinositol 3-phosphate (PtdIns3P)-binding or via its interaction with RAB14. Ref.9 Ref.13
Tissue specificity	Primarily expressed in brain. Also present in kidney, liver, intestine, placenta, leukocytes, heart and skeletal muscle (at protein level). Ref.9
Domain	The PX domain mediates binding to phosphatidylinositol 3-phosphate (PtdIns3P), phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2), phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3). Does not bind phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Ref.9 Ref.13
Post-translational modification	Isoform 4 is ubiquitinated at Lys-685.
Sequence similarities	Belongs to the kinesin-like protein family. Contains 1 FHA domain. Contains 1 kinesin-motor domain. Contains 1 PX (phox homology) domain.
Sequence caution	The sequence BAA90971.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAA90971.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence BAB13416.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAB15530.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAI43062.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI43179.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm Cytoskeleton Endosome Membrane Microtubule
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Ligand	ATP-binding Lipid-binding Nucleotide-binding
Molecular function	Motor protein
PTM	Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to endosome transport Inferred from sequence or structural similarity. Source: UniProtKB early endosome to late endosome transport Inferred from mutant phenotype Ref.9. Source: UniProtKB endoderm development Inferred from sequence or structural similarity. Source: UniProtKB epidermal growth factor receptor signaling pathway Inferred from mutant phenotype Ref.9. Source: UniProtKB fibroblast growth factor receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB formation of primary germ layer Inferred from sequence or structural similarity. Source: UniProtKB microtubule-based movement Inferred from electronic annotation. Source: InterPro receptor catabolic process Inferred from mutant phenotype Ref.9. Source: UniProtKB regulation of receptor recycling Inferred from mutant phenotype Ref.9. Source: UniProtKB
Cellular_component	early endosome Inferred from direct assay Ref.9. Source: UniProtKB early endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW microtubule associated complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylinositol-3,4,5-trisphosphate binding Inferred from direct assay Ref.9Ref.13. Source: UniProtKB phosphatidylinositol-3,4-bisphosphate binding Inferred from direct assay Ref.9Ref.13. Source: UniProtKB phosphatidylinositol-3,5-bisphosphate binding Inferred from direct assay Ref.13. Source: UniProtKB phosphatidylinositol-3-phosphate binding Inferred from direct assay Ref.9. Source: UniProtKB plus-end-directed microtubule motor activity Inferred from direct assay Ref.9. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96L93-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96L93-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1167-1317: RMVSRSLGAN...VFDYSSHGTG → LCRDLLCVLM...LWLISHLTRL

Note: Ubiquitinated at position 685.

Isoform 3 (identifier: Q96L93-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1208-1237: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q96L93-5)

The sequence of this isoform differs from the canonical sequence as follows:
     685-840: Missing.

Note: No experimental confirmation available.

Isoform 5 (identifier: Q96L93-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1167-1207: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1317

1317

Kinesin-like protein KIF16B

PRO_0000125466

Regions

Domain

1 – 365

365

Kinesin-motor

Domain

478 – 529

52

FHA

Domain

1182 – 1296

115

PX

Nucleotide binding

102 – 109

8

ATP Potential

Coiled coil

370 – 425

56

Potential

Coiled coil

595 – 882

288

Potential

Coiled coil

936 – 1087

152

Potential

Compositional bias

597 – 1070

474

Glu-rich

Natural variations

Alternative sequence

685 – 840

156

Missing in isoform 4.

VSP_015858

Alternative sequence

1167 – 1317

151

RMVSR…SHGTG → LCRDLLCVLMPEPDAAACAN HPLLQQDLVQLSLDWKTEIP DLVLPNGVQVSSKFQTTLVD MIYFLHGNMEVNVPSLAEVQ LLLYTTVKVMGDSGHDQCQS LVLLNTHIALVKEDCVFYPR IRSRNIPPPGAQFDVIKCHA LSEFRCVVVPEKKNVSTVEL VFLQKLKPSVGSRNSPPEHL QEAPNVQLFTTPLYLQGSQN VAPEVWKLTFNSQDEALWLI SHLTRL in isoform 2.

VSP_010851

Alternative sequence

1167 – 1207

41

Missing in isoform 5.

VSP_041318

Alternative sequence

1208 – 1237

30

Missing in isoform 3.

VSP_010852

Natural variant

772

1

K → T in a breast cancer sample; somatic mutation. Ref.14

VAR_036218

Natural variant

810

1

G → R.
Corresponds to variant rs2236145 [ dbSNP | Ensembl ].

VAR_049700

Natural variant

824

1

R → S.
Corresponds to variant rs2236144 [ dbSNP | Ensembl ].

VAR_019396

Natural variant

999

1

K → N.
Corresponds to variant rs8116503 [ dbSNP | Ensembl ].

VAR_065248

Natural variant

1027

1

M → T. Ref.1 Ref.2 Ref.6
Corresponds to variant rs6034464 [ dbSNP | Ensembl ].

VAR_019397

Natural variant

1119

1

N → S.
Corresponds to variant rs8123195 [ dbSNP | Ensembl ].

VAR_019398

Experimental info

Mutagenesis

109

1

S → A: Impairs plus end-directed microtubule-dependent motor activity, leading to impair EGFR recycling. Ref.9

Mutagenesis

1220

1

R → A: Abolishes PtdIns(3)P-binding. Ref.13

Mutagenesis

1225

1

R → A: Induces a 3-fold decrease in PtdIns(3)P-binding; when associated with A-1229 and A-1232. Ref.13

Mutagenesis

1229

1

K → A: Induces a 3-fold decrease in PtdIns(3)P-binding; when associated with A-1225 and A-1232. Ref.13

Mutagenesis

1232

1

K → A: Induces a 3-fold decrease in PtdIns(3)P-binding; when associated with A-1225 and A-1229. Ref.13

Mutagenesis

1248

1

L → A: Induces a 7-fold decrease in PtdIns(3)P-binding and abolishes endosome localization. Induces a 25-fold decrease in PtdIns(3)P-binding; when associated with A-1249. Ref.13

Mutagenesis

1248

1

L → V: Induces a 6-fold decrease in PtdIns(3)P-binding. Ref.13

Mutagenesis

1249

1

F → A: Induces a 5-fold decrease in PtdIns(3)P-binding and abolishes endosome localization. Induces a 25-fold decrease in PtdIns(3)P-binding; when associated with A-1248. Ref.13

Mutagenesis

1260

1

R → A: Induces a 30-fold decrease in PtdIns(3)P-binding. Ref.13

Sequence conflict

18

1

K → E in AK095322. Ref.7

Sequence conflict

274

1

N → D in CAI46105. Ref.2

Sequence conflict

583

1

R → H in BAA90971. Ref.7

Sequence conflict

649

1

L → H in CAI46266. Ref.2

Sequence conflict

1056 – 1057

2

EQ → SE in AAH34984. Ref.6

Sequence conflict

1100

1

S → N in CAI46105. Ref.2

Sequence conflict

1108

1

S → L in AAH34984. Ref.6

Sequence conflict

1244

1

P → A in AAO17292. Ref.3

Sequence conflict

1244

1

P → A in BAB15530. Ref.7

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1317

Helix Strand Turn

Details...

Beta strand

1184 – 1196

13

Beta strand

1201 – 1210

10

Beta strand

1213 – 1219

7

Helix

1221 – 1234

14

Helix

1236 – 1240

5

Helix

1254 – 1277

24

Helix

1294 – 1300

7

Helix

1302 – 1304

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 19, 2004. Version 2. Checksum: 278E9CB88D9C04DF	FASTA	1,317	152,011
	10 20 30 40 50 60 MASVKVAVRV RPMNRREKDL EAKFIIQMEK SKTTITNLKI PEGGTGDSGR ERTKTFTYDF 70 80 90 100 110 120 SFYSADTKSP DYVSQEMVFK TLGTDVVKSA FEGYNACVFA YGQTGSGKSY TMMGNSGDSG 130 140 150 160 170 180 LIPRICEGLF SRINETTRWD EASFRTEVSY LEIYNERVRD LLRRKSSKTF NLRVREHPKE 190 200 210 220 230 240 GPYVEDLSKH LVQNYGDVEE LMDAGNINRT TAATGMNDVS SRSHAIFTIK FTQAKFDSEM 250 260 270 280 290 300 PCETVSKIHL VDLAGSERAD ATGATGVRLK EGGNINKSLV TLGNVISALA DLSQDAANTL 310 320 330 340 350 360 AKKKQVFVPY RDSVLTWLLK DSLGGNSKTI MIATISPADV NYGETLSTLR YANRAKNIIN 370 380 390 400 410 420 KPTINEDANV KLIRELRAEI ARLKTLLAQG NQIALLDSPT ALSMEEKLQQ NEARVQELTK 430 440 450 460 470 480 EWTNKWNETQ NILKEQTLAL RKEGIGVVLD SELPHLIGID DDLLSTGIIL YHLKEGQTYV 490 500 510 520 530 540 GRDDASTEQD IVLHGLDLES EHCIFENIGG TVTLIPLSGS QCSVNGVQIV EATHLNQGAV 550 560 570 580 590 600 ILLGRTNMFR FNHPKEAAKL REKRKSGLLS SFSLSMTDLS KSRENLSAVM LYNPGLEFER 610 620 630 640 650 660 QQREELEKLE SKRKLIEEME EKQKSDKAEL ERMQQEVETQ RKETEIVQLQ IRKQEESLKR 670 680 690 700 710 720 RSFHIENKLK DLLAEKEKFE EERLREQQEI ELQKKRQEEE TFLRVQEELQ RLKELNNNEK 730 740 750 760 770 780 AEKFQIFQEL DQLQKEKDEQ YAKLELEKKR LEEQEKEQVM LVAHLEEQLR EKQEMIQLLR 790 800 810 820 830 840 RGEVQWVEEE KRDLEGIRES LLRVKEARAG GDEDGEELEK AQLRFFEFKR RQLVKLVNLE 850 860 870 880 890 900 KDLVQQKDIL KKEVQEEQEI LECLKCEHDK ESRLLEKHDE SVTDVTEVPQ DFEKIKPVEY 910 920 930 940 950 960 RLQYKERQLQ YLLQNHLPTL LEEKQRAFEI LDRGPLSLDN TLYQVEKEME EKEEQLAQYQ 970 980 990 1000 1010 1020 ANANQLQKLQ ATFEFTANIA RQEEKVRKKE KEILESREKQ QREALERALA RLERRHSALQ 1030 1040 1050 1060 1070 1080 RHSTLGMEIE EQRQKLASLN SGSREQSGLQ ASLEAEQEAL EKDQERLEYE IQQLKQKIYE 1090 1100 1110 1120 1130 1140 VDGVQKDHHG TLEGKVASSS LPVSAEKSHL VPLMDARINA YIEEEVQRRL QDLHRVISEG 1150 1160 1170 1180 1190 1200 CSTSADTMKD NEKLHNGTIQ RKLKYERMVS RSLGANPDDL KDPIKISIPR YVLCGQGKDA 1210 1220 1230 1240 1250 1260 HFEFEVKITV LDETWTVFRR YSRFREMHKT LKLKYAELAA LEFPPKKLFG NKDERVIAER 1270 1280 1290 1300 1310 RSHLEKYLRD FFSVMLQSAT SPLHINKVGL TLSKHTICEF SPFFKKGVFD YSSHGTG
	Isoform 2 [UniParc]. Checksum: 85A4365F83AADDB9	FASTA	1,392	159,831
	10 20 30 40 50 60 MASVKVAVRV RPMNRREKDL EAKFIIQMEK SKTTITNLKI PEGGTGDSGR ERTKTFTYDF 70 80 90 100 110 120 SFYSADTKSP DYVSQEMVFK TLGTDVVKSA FEGYNACVFA YGQTGSGKSY TMMGNSGDSG 130 140 150 160 170 180 LIPRICEGLF SRINETTRWD EASFRTEVSY LEIYNERVRD LLRRKSSKTF NLRVREHPKE 190 200 210 220 230 240 GPYVEDLSKH LVQNYGDVEE LMDAGNINRT TAATGMNDVS SRSHAIFTIK FTQAKFDSEM 250 260 270 280 290 300 PCETVSKIHL VDLAGSERAD ATGATGVRLK EGGNINKSLV TLGNVISALA DLSQDAANTL 310 320 330 340 350 360 AKKKQVFVPY RDSVLTWLLK DSLGGNSKTI MIATISPADV NYGETLSTLR YANRAKNIIN 370 380 390 400 410 420 KPTINEDANV KLIRELRAEI ARLKTLLAQG NQIALLDSPT ALSMEEKLQQ NEARVQELTK 430 440 450 460 470 480 EWTNKWNETQ NILKEQTLAL RKEGIGVVLD SELPHLIGID DDLLSTGIIL YHLKEGQTYV 490 500 510 520 530 540 GRDDASTEQD IVLHGLDLES EHCIFENIGG TVTLIPLSGS QCSVNGVQIV EATHLNQGAV 550 560 570 580 590 600 ILLGRTNMFR FNHPKEAAKL REKRKSGLLS SFSLSMTDLS KSRENLSAVM LYNPGLEFER 610 620 630 640 650 660 QQREELEKLE SKRKLIEEME EKQKSDKAEL ERMQQEVETQ RKETEIVQLQ IRKQEESLKR 670 680 690 700 710 720 RSFHIENKLK DLLAEKEKFE EERLREQQEI ELQKKRQEEE TFLRVQEELQ RLKELNNNEK 730 740 750 760 770 780 AEKFQIFQEL DQLQKEKDEQ YAKLELEKKR LEEQEKEQVM LVAHLEEQLR EKQEMIQLLR 790 800 810 820 830 840 RGEVQWVEEE KRDLEGIRES LLRVKEARAG GDEDGEELEK AQLRFFEFKR RQLVKLVNLE 850 860 870 880 890 900 KDLVQQKDIL KKEVQEEQEI LECLKCEHDK ESRLLEKHDE SVTDVTEVPQ DFEKIKPVEY 910 920 930 940 950 960 RLQYKERQLQ YLLQNHLPTL LEEKQRAFEI LDRGPLSLDN TLYQVEKEME EKEEQLAQYQ 970 980 990 1000 1010 1020 ANANQLQKLQ ATFEFTANIA RQEEKVRKKE KEILESREKQ QREALERALA RLERRHSALQ 1030 1040 1050 1060 1070 1080 RHSTLGMEIE EQRQKLASLN SGSREQSGLQ ASLEAEQEAL EKDQERLEYE IQQLKQKIYE 1090 1100 1110 1120 1130 1140 VDGVQKDHHG TLEGKVASSS LPVSAEKSHL VPLMDARINA YIEEEVQRRL QDLHRVISEG 1150 1160 1170 1180 1190 1200 CSTSADTMKD NEKLHNGTIQ RKLKYELCRD LLCVLMPEPD AAACANHPLL QQDLVQLSLD 1210 1220 1230 1240 1250 1260 WKTEIPDLVL PNGVQVSSKF QTTLVDMIYF LHGNMEVNVP SLAEVQLLLY TTVKVMGDSG 1270 1280 1290 1300 1310 1320 HDQCQSLVLL NTHIALVKED CVFYPRIRSR NIPPPGAQFD VIKCHALSEF RCVVVPEKKN 1330 1340 1350 1360 1370 1380 VSTVELVFLQ KLKPSVGSRN SPPEHLQEAP NVQLFTTPLY LQGSQNVAPE VWKLTFNSQD 1390 EALWLISHLT RL
	Isoform 3 [UniParc]. Checksum: 9BA79E2857090F5F	FASTA	1,287	148,211
	10 20 30 40 50 60 MASVKVAVRV RPMNRREKDL EAKFIIQMEK SKTTITNLKI PEGGTGDSGR ERTKTFTYDF 70 80 90 100 110 120 SFYSADTKSP DYVSQEMVFK TLGTDVVKSA FEGYNACVFA YGQTGSGKSY TMMGNSGDSG 130 140 150 160 170 180 LIPRICEGLF SRINETTRWD EASFRTEVSY LEIYNERVRD LLRRKSSKTF NLRVREHPKE 190 200 210 220 230 240 GPYVEDLSKH LVQNYGDVEE LMDAGNINRT TAATGMNDVS SRSHAIFTIK FTQAKFDSEM 250 260 270 280 290 300 PCETVSKIHL VDLAGSERAD ATGATGVRLK EGGNINKSLV TLGNVISALA DLSQDAANTL 310 320 330 340 350 360 AKKKQVFVPY RDSVLTWLLK DSLGGNSKTI MIATISPADV NYGETLSTLR YANRAKNIIN 370 380 390 400 410 420 KPTINEDANV KLIRELRAEI ARLKTLLAQG NQIALLDSPT ALSMEEKLQQ NEARVQELTK 430 440 450 460 470 480 EWTNKWNETQ NILKEQTLAL RKEGIGVVLD SELPHLIGID DDLLSTGIIL YHLKEGQTYV 490 500 510 520 530 540 GRDDASTEQD IVLHGLDLES EHCIFENIGG TVTLIPLSGS QCSVNGVQIV EATHLNQGAV 550 560 570 580 590 600 ILLGRTNMFR FNHPKEAAKL REKRKSGLLS SFSLSMTDLS KSRENLSAVM LYNPGLEFER 610 620 630 640 650 660 QQREELEKLE SKRKLIEEME EKQKSDKAEL ERMQQEVETQ RKETEIVQLQ IRKQEESLKR 670 680 690 700 710 720 RSFHIENKLK DLLAEKEKFE EERLREQQEI ELQKKRQEEE TFLRVQEELQ RLKELNNNEK 730 740 750 760 770 780 AEKFQIFQEL DQLQKEKDEQ YAKLELEKKR LEEQEKEQVM LVAHLEEQLR EKQEMIQLLR 790 800 810 820 830 840 RGEVQWVEEE KRDLEGIRES LLRVKEARAG GDEDGEELEK AQLRFFEFKR RQLVKLVNLE 850 860 870 880 890 900 KDLVQQKDIL KKEVQEEQEI LECLKCEHDK ESRLLEKHDE SVTDVTEVPQ DFEKIKPVEY 910 920 930 940 950 960 RLQYKERQLQ YLLQNHLPTL LEEKQRAFEI LDRGPLSLDN TLYQVEKEME EKEEQLAQYQ 970 980 990 1000 1010 1020 ANANQLQKLQ ATFEFTANIA RQEEKVRKKE KEILESREKQ QREALERALA RLERRHSALQ 1030 1040 1050 1060 1070 1080 RHSTLGMEIE EQRQKLASLN SGSREQSGLQ ASLEAEQEAL EKDQERLEYE IQQLKQKIYE 1090 1100 1110 1120 1130 1140 VDGVQKDHHG TLEGKVASSS LPVSAEKSHL VPLMDARINA YIEEEVQRRL QDLHRVISEG 1150 1160 1170 1180 1190 1200 CSTSADTMKD NEKLHNGTIQ RKLKYERMVS RSLGANPDDL KDPIKISIPR YVLCGQGKDA 1210 1220 1230 1240 1250 1260 HFEFEVKLAA LEFPPKKLFG NKDERVIAER RSHLEKYLRD FFSVMLQSAT SPLHINKVGL 1270 1280 TLSKHTICEF SPFFKKGVFD YSSHGTG
	Isoform 4 [UniParc]. Checksum: 078AB4C77FBAA4C3	FASTA	1,161	132,866
	10 20 30 40 50 60 MASVKVAVRV RPMNRREKDL EAKFIIQMEK SKTTITNLKI PEGGTGDSGR ERTKTFTYDF 70 80 90 100 110 120 SFYSADTKSP DYVSQEMVFK TLGTDVVKSA FEGYNACVFA YGQTGSGKSY TMMGNSGDSG 130 140 150 160 170 180 LIPRICEGLF SRINETTRWD EASFRTEVSY LEIYNERVRD LLRRKSSKTF NLRVREHPKE 190 200 210 220 230 240 GPYVEDLSKH LVQNYGDVEE LMDAGNINRT TAATGMNDVS SRSHAIFTIK FTQAKFDSEM 250 260 270 280 290 300 PCETVSKIHL VDLAGSERAD ATGATGVRLK EGGNINKSLV TLGNVISALA DLSQDAANTL 310 320 330 340 350 360 AKKKQVFVPY RDSVLTWLLK DSLGGNSKTI MIATISPADV NYGETLSTLR YANRAKNIIN 370 380 390 400 410 420 KPTINEDANV KLIRELRAEI ARLKTLLAQG NQIALLDSPT ALSMEEKLQQ NEARVQELTK 430 440 450 460 470 480 EWTNKWNETQ NILKEQTLAL RKEGIGVVLD SELPHLIGID DDLLSTGIIL YHLKEGQTYV 490 500 510 520 530 540 GRDDASTEQD IVLHGLDLES EHCIFENIGG TVTLIPLSGS QCSVNGVQIV EATHLNQGAV 550 560 570 580 590 600 ILLGRTNMFR FNHPKEAAKL REKRKSGLLS SFSLSMTDLS KSRENLSAVM LYNPGLEFER 610 620 630 640 650 660 QQREELEKLE SKRKLIEEME EKQKSDKAEL ERMQQEVETQ RKETEIVQLQ IRKQEESLKR 670 680 690 700 710 720 RSFHIENKLK DLLAEKEKFE EERLKDLVQQ KDILKKEVQE EQEILECLKC EHDKESRLLE 730 740 750 760 770 780 KHDESVTDVT EVPQDFEKIK PVEYRLQYKE RQLQYLLQNH LPTLLEEKQR AFEILDRGPL 790 800 810 820 830 840 SLDNTLYQVE KEMEEKEEQL AQYQANANQL QKLQATFEFT ANIARQEEKV RKKEKEILES 850 860 870 880 890 900 REKQQREALE RALARLERRH SALQRHSTLG MEIEEQRQKL ASLNSGSREQ SGLQASLEAE 910 920 930 940 950 960 QEALEKDQER LEYEIQQLKQ KIYEVDGVQK DHHGTLEGKV ASSSLPVSAE KSHLVPLMDA 970 980 990 1000 1010 1020 RINAYIEEEV QRRLQDLHRV ISEGCSTSAD TMKDNEKLHN GTIQRKLKYE RMVSRSLGAN 1030 1040 1050 1060 1070 1080 PDDLKDPIKI SIPRYVLCGQ GKDAHFEFEV KITVLDETWT VFRRYSRFRE MHKTLKLKYA 1090 1100 1110 1120 1130 1140 ELAALEFPPK KLFGNKDERV IAERRSHLEK YLRDFFSVML QSATSPLHIN KVGLTLSKHT 1150 1160 ICEFSPFFKK GVFDYSSHGT G
	Isoform 5 [UniParc]. Checksum: 430E508AEE6E2E12	FASTA	1,276	147,398
	10 20 30 40 50 60 MASVKVAVRV RPMNRREKDL EAKFIIQMEK SKTTITNLKI PEGGTGDSGR ERTKTFTYDF 70 80 90 100 110 120 SFYSADTKSP DYVSQEMVFK TLGTDVVKSA FEGYNACVFA YGQTGSGKSY TMMGNSGDSG 130 140 150 160 170 180 LIPRICEGLF SRINETTRWD EASFRTEVSY LEIYNERVRD LLRRKSSKTF NLRVREHPKE 190 200 210 220 230 240 GPYVEDLSKH LVQNYGDVEE LMDAGNINRT TAATGMNDVS SRSHAIFTIK FTQAKFDSEM 250 260 270 280 290 300 PCETVSKIHL VDLAGSERAD ATGATGVRLK EGGNINKSLV TLGNVISALA DLSQDAANTL 310 320 330 340 350 360 AKKKQVFVPY RDSVLTWLLK DSLGGNSKTI MIATISPADV NYGETLSTLR YANRAKNIIN 370 380 390 400 410 420 KPTINEDANV KLIRELRAEI ARLKTLLAQG NQIALLDSPT ALSMEEKLQQ NEARVQELTK 430 440 450 460 470 480 EWTNKWNETQ NILKEQTLAL RKEGIGVVLD SELPHLIGID DDLLSTGIIL YHLKEGQTYV 490 500 510 520 530 540 GRDDASTEQD IVLHGLDLES EHCIFENIGG TVTLIPLSGS QCSVNGVQIV EATHLNQGAV 550 560 570 580 590 600 ILLGRTNMFR FNHPKEAAKL REKRKSGLLS SFSLSMTDLS KSRENLSAVM LYNPGLEFER 610 620 630 640 650 660 QQREELEKLE SKRKLIEEME EKQKSDKAEL ERMQQEVETQ RKETEIVQLQ IRKQEESLKR 670 680 690 700 710 720 RSFHIENKLK DLLAEKEKFE EERLREQQEI ELQKKRQEEE TFLRVQEELQ RLKELNNNEK 730 740 750 760 770 780 AEKFQIFQEL DQLQKEKDEQ YAKLELEKKR LEEQEKEQVM LVAHLEEQLR EKQEMIQLLR 790 800 810 820 830 840 RGEVQWVEEE KRDLEGIRES LLRVKEARAG GDEDGEELEK AQLRFFEFKR RQLVKLVNLE 850 860 870 880 890 900 KDLVQQKDIL KKEVQEEQEI LECLKCEHDK ESRLLEKHDE SVTDVTEVPQ DFEKIKPVEY 910 920 930 940 950 960 RLQYKERQLQ YLLQNHLPTL LEEKQRAFEI LDRGPLSLDN TLYQVEKEME EKEEQLAQYQ 970 980 990 1000 1010 1020 ANANQLQKLQ ATFEFTANIA RQEEKVRKKE KEILESREKQ QREALERALA RLERRHSALQ 1030 1040 1050 1060 1070 1080 RHSTLGMEIE EQRQKLASLN SGSREQSGLQ ASLEAEQEAL EKDQERLEYE IQQLKQKIYE 1090 1100 1110 1120 1130 1140 VDGVQKDHHG TLEGKVASSS LPVSAEKSHL VPLMDARINA YIEEEVQRRL QDLHRVISEG 1150 1160 1170 1180 1190 1200 CSTSADTMKD NEKLHNGTIQ RKLKYEITVL DETWTVFRRY SRFREMHKTL KLKYAELAAL 1210 1220 1230 1240 1250 1260 EFPPKKLFGN KDERVIAERR SHLEKYLRDF FSVMLQSATS PLHINKVGLT LSKHTICEFS 1270 PFFKKGVFDY SSHGTG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Nakayama M., Hirosawa M., Ohara O. DNA Res. 7:273-281(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT THR-1027. Tissue: Brain.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 557-1317 (ISOFORM 4), VARIANT THR-1027. Tissue: Adipose tissue and Salivary gland.
[3]	Guo J.H. Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 916-1317 (ISOFORM 5), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1056-1317 (ISOFORM 3), VARIANT THR-1027. Tissue: Hippocampus and Liver.
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-948 (ISOFORMS 1/2/3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1085-1317 (ISOFORMS 1/4). Tissue: Colon, Lung and Tongue.
[8]	"A new member (SNX23) of the sorting nexin family." Hong W. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1114-1317 (ISOFORMS 1/4).
[9]	"Modulation of receptor recycling and degradation by the endosomal kinesin KIF16B." Hoepfner S., Severin F., Cabezas A., Habermann B., Runge A., Gillooly D., Stenmark H., Zerial M. Cell 121:437-450(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DOMAIN PX, PHOSPHATIDYLINOSITOL-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF SER-109.
[10]	"The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells." Vasilescu J., Zweitzig D.R., Denis N.J., Smith J.C., Ethier M., Haines D.S., Figeys D. J. Proteome Res. 6:298-305(2007) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-685 (ISOFORM 4), MASS SPECTROMETRY. Tissue: Lung adenocarcinoma.
[11]	"PTPD1 supports receptor stability and mitogenic signaling in bladder cancer cells." Carlucci A., Porpora M., Garbi C., Galgani M., Santoriello M., Mascolo M., di Lorenzo D., Altieri V., Quarto M., Terracciano L., Gottesman M.E., Insabato L., Feliciello A. J. Biol. Chem. 285:39260-39270(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PTPN21.
[12]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"The structural basis of novel endosome anchoring activity of KIF16B kinesin." Blatner N.R., Wilson M.I., Lei C., Hong W., Murray D., Williams R.L., Cho W. EMBO J. 26:3709-3719(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1179-1312, SUBCELLULAR LOCATION, PHOSPHATIDYLINOSITOL-BINDING, DOMAIN PX, MUTAGENESIS OF ARG-1220; ARG-1225; LYS-1229; LYS-1232; LEU-1248; PHE-1249 AND ARG-1260.
[14]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-772.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB046810 mRNA. Translation: BAB13416.2. Different initiation.
BX647572 mRNA. Translation: CAI46105.1.
BX648426 mRNA. Translation: CAI46266.1.
AY166853 mRNA. Translation: AAO17292.1.
AL049794, AL117376, AL118509 Genomic DNA. Translation: CAI22745.2.
AL117376, AL049794, AL118509 Genomic DNA. Translation: CAI43180.2.
AL117376 Genomic DNA. Translation: CAI43181.2.
AL118509, AL049794, AL117376 Genomic DNA. Translation: CAI43063.2.
AL118509, AL049794, AL117376 Genomic DNA. Translation: CAM28305.1.
AL049794, AL117376, AL118509 Genomic DNA. Translation: CAM28307.1.
AL118509, AL117376 Genomic DNA. Translation: CAI43062.1. Sequence problems.
AL117376, AL118509 Genomic DNA. Translation: CAI43179.1. Sequence problems.
CH471133 Genomic DNA. Translation: EAX10290.1.
BC034984 mRNA. Translation: AAH34984.2.
BC110317 mRNA. Translation: AAI10318.1.
BC150261 mRNA. Translation: AAI50262.1.
AY044654 mRNA. Translation: AAK98768.1.
AK000142 mRNA. Translation: BAA90971.1. Sequence problems.
AK026698 mRNA. Translation: BAB15530.1. Different initiation.
AK095322 mRNA. No translation available.

IPI

IPI00452246.
IPI00452247.
IPI00452250.
IPI00651625.
IPI00747865.

RefSeq

NP_001186794.1. NM_001199865.1.
NP_001186795.1. NM_001199866.1.
NP_078980.3. NM_024704.4.

UniGene

Hs.101774.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2V14	X-ray	2.20	A	1179-1312	[»]

ProteinModelPortal

Q96L93.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96L93. 1 interaction.

MINT

MINT-5225330.

PTM databases

PhosphoSite

Q96L93.

Polymorphism databases

DMDM

50403793.

Proteomic databases

PaxDb

Q96L93.

PRIDE

Q96L93.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000354981; ENSP00000347076; ENSG00000089177.
ENST00000355755; ENSP00000347995; ENSG00000089177.
ENST00000408042; ENSP00000384164; ENSG00000089177.

GeneID

55614.

KEGG

hsa:55614.

UCSC

uc002wpe.1. human.
uc002wpf.1. human.
uc002wpg.2. human.
uc010gci.2. human.

Organism-specific databases

CTD

55614.

GeneCards

GC20M016201.

HGNC

HGNC:15869. KIF16B.

HPA

HPA051198.

neXtProt

NX_Q96L93.

PharmGKB

PA162393227.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5059.

HOVERGEN

HBG099335.

KO

K10392.

OMA

IAERRSH.

OrthoDB

EOG4255RZ.

Enzyme and pathway databases

BRENDA

3.6.4.4. 2681.

Gene expression databases

Bgee

Q96L93.

CleanEx

HS_KIF16B.

Genevestigator

Q96L93.

GermOnline

ENSG00000089177. Homo sapiens.

Family and domain databases

Gene3D

2.60.200.20. 1 hit.
3.30.1520.10. 1 hit.
3.40.850.10. 1 hit.

InterPro

IPR000253. FHA_dom.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR001683. Phox.
IPR008984. SMAD_FHA_domain.
[Graphical view]

Pfam

PF00498. FHA. 1 hit.
PF00225. Kinesin. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]

PRINTS

PR00380. KINESINHEAVY.

SMART

SM00240. FHA. 1 hit.
SM00129. KISc. 1 hit.
SM00312. PX. 1 hit.
[Graphical view]

SUPFAM

SSF64268. PX. 1 hit.
SSF49879. SMAD_FHA. 1 hit.

PROSITE

PS50006. FHA_DOMAIN. False negative.
PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit.
PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit.
PS50195. PX. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

KIF16B. human.

EvolutionaryTrace

Q96L93.

GenomeRNAi

55614.

NextBio

60192.

Entry information

Entry name

KI16B_HUMAN

Accession

Primary (citable) accession number: Q96L93
Secondary accession number(s): A6NKJ9 , A7E2A8, B1AKG3, B1AKT7, C9JDN5, C9JI52, C9JSM8, C9JWJ7, Q2TBF5, Q5HYC0, Q5HYK1, Q5JWW3, Q5TFK5, Q86VL9, Q86YS5, Q8IYU0, Q9BQJ8, Q9BQM0, Q9BQM1, Q9BQM5, Q9H5U0, Q9HCI2, Q9NXN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.