Q96L58 (B3GT6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1,3-galactosyltransferase 6
Short name=Beta-1,3-GalTase 6
Short name=Beta3Gal-T6
Short name=Beta3GalT6
EC=2.4.1.134

Alternative name(s):
GAG GalTII
Galactosyltransferase II
Galactosylxylosylprotein 3-beta-galactosyltransferase
UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 6

Gene names

Name:

B3GALT6

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. Has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. Has no activity towards substrates with terminal glucosamine or galactosamine residues. Ref.1
Catalytic activity	UDP-galactose + 4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.
Cofactor	Manganese. Ref.3
Pathway	Glycan metabolism; chondroitin sulfate biosynthesis. Glycan metabolism; heparan sulfate biosynthesis.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein Ref.1.
Tissue specificity	Ubiquitous. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 31 family.
Caution	Ref.3 describes the wrong protein; the cDNAs used had been switched inadvertently.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycosaminoglycan biosynthetic process Inferred from mutant phenotype Ref.1. Source: UniProtKB protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi medial cisterna Inferred from direct assay Ref.1. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosylxylosylprotein 3-beta-galactosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Beta-1,3-galactosyltransferase 6		PRO_0000219168
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 34	23	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	35 – 329	295	Lumenal Potential
	Compositional bias	47 – 52	6	Poly-Pro
Amino acid modifications
	Glycosylation	131	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	174	1	E → D. Corresponds to variant rs12085009 [ dbSNP | Ensembl ].		VAR_059317
Experimental info
	Sequence conflict	2	1	K → N in AAL11442. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q96L58 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: F4E2A219B1DC11F7	FASTA	329	37,138
	10 20 30 40 50 60 MKLLRRAWRR RAALGLGTLA LCGAALLYLA RCAAEPGDPR AMSGRSPPPP APARAAAFLA 70 80 90 100 110 120 VLVASAPRAA ERRSVIRSTW LARRGAPGDV WARFAVGTAG LGAEERRALE REQARHGDLL 130 140 150 160 170 180 LLPALRDAYE NLTAKVLAML AWLDEHVAFE FVLKADDDSF ARLDALLAEL RAREPARRRR 190 200 210 220 230 240 LYWGFFSGRG RVKPGGRWRE AAWQLCDYYL PYALGGGYVL SADLVHYLRL SRDYLRAWHS 250 260 270 280 290 300 EDVSLGAWLA PVDVQREHDP RFDTEYRSRG CSNQYLVTHK QSLEDMLEKH ATLAREGRLC 310 320 KREVQLRLSY VYDWSAPPSQ CCQRREGIP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biosynthesis of the linkage region of glycosaminoglycans: cloning and activity of galactosyltransferase II, the sixth member of the beta 1,3-galactosyltransferase family (beta 3GalT6)." Bai X., Zhou D., Brown J.R., Crawford B.E., Hennet T., Esko J.D. J. Biol. Chem. 276:48189-48195(2001) [PubMed: 11551958] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Fetal brain.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"A beta-1,3-N-acetylglucosaminyltransferase with poly-N-acetyllactosamine synthase activity is structurally related to beta-1,3-galactosyltransferases." Zhou D., Dinter A., Gutierrez Gallego R., Kamerling J.P., Vliegenthart J.F.G., Berger E.G., Hennet T. Proc. Natl. Acad. Sci. U.S.A. 96:406-411(1999) [PubMed: 9892646] [Abstract] Cited for: COFACTOR.
[4]	Erratum Zhou D., Dinter A., Gutierrez Gallego R., Kamerling J.P., Vliegenthart J.F.G., Berger E.G., Hennet T. Proc. Natl. Acad. Sci. U.S.A. 97:11673-11673(2000)
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Beta-1,3-galactosyltransferase 6

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY050570 mRNA. Translation: AAL11442.1. AL162741 Genomic DNA. Translation: CAI23255.1.
IPI	IPI00064848.
RefSeq	NP_542172.2. NM_080605.3.
UniGene	Hs.284284.
3D structure databases
ProteinModelPortal	Q96L58.
ModBase	Search...
Protein-protein interaction databases
STRING	Q96L58.
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Polymorphism databases
DMDM	61211870.
Proteomic databases
PRIDE	Q96L58.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000379198; ENSP00000368496; ENSG00000176022.
GeneID	126792.
KEGG	hsa:126792.
NMPDR	fig|9606.3.peg.34.
UCSC	uc001adk.1. human.
Organism-specific databases
CTD	126792.
GeneCards	GC01P001157.
H-InvDB	HIX0028666.
HGNC	HGNC:17978. B3GALT6.
neXtProt	NX_Q96L58.
PharmGKB	PA25214.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00530000063810.
HOGENOM	HBG714704.
HOVERGEN	HBG050652.
InParanoid	Q96L58.
OMA	HEGRLCK.
OrthoDB	EOG4J6RR8.
PhylomeDB	Q96L58.
Enzyme and pathway databases
BioCyc	MetaCyc:HS10991-MONOMER.
Gene expression databases
ArrayExpress	Q96L58.
Bgee	Q96L58.
CleanEx	HS_B3GALT6.
Genevestigator	Q96L58.
GermOnline	ENSG00000176022. Homo sapiens.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
KO	K00734.
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	81920.

Entry information

Entry name

B3GT6_HUMAN

Accession

Primary (citable) accession number: Q96L58
Secondary accession number(s): Q5T7M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.