Reviewed,
UniProtKB/Swiss-Prot Q96L34 (MARK4_HUMAN)
Last modified
December 15, 2009.
Version 87.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: MAP/microtubule affinity-regulating kinase 4 EC=2.7.11.1 Alternative name(s): MAP/microtubule affinity-regulating kinase-like 1 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 752 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Tissue specificity | |
| Sequence similarities | Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily. Contains 1 KA1 (kinase-associated) domain. Contains 1 protein kinase domain. Contains 1 UBA domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| CDC42 | P60953 | 1 | EBI-302319,EBI-81752 | |
| PARD6G | Q9BYG4 | 1 | EBI-302319,EBI-295417 | |
| PNMA1 | Q8ND90 | 1 | EBI-302319,EBI-302345 | |
| PRKCI | P41743 | 1 | EBI-302319,EBI-286199 | |
| STK11 | Q15831 | 2 | EBI-302319,EBI-306838 | |
| TUBG1 | P23258 | 1 | EBI-302319,EBI-302589 | |
| YWHAH | Q04917 | 1 | EBI-302319,EBI-306940 |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q96L34-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q96L34-2) Also known as: MARKL1S; The sequence of this isoform differs from the canonical sequence as follows: 628-752: ADEPERIGGP...VTRISNDLEL → TLDPSKRQNS...PPGCSDSPGV |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 752 | 752 | MAP/microtubule affinity-regulating kinase 4 | PRO_0000086307 | |||||
Regions | |||||||||
| Domain | 59 – 310 | 252 | Protein kinase | ||||||
| Domain | 324 – 368 | 45 | UBA | ||||||
| Domain | 703 – 752 | 50 | KA1 | ||||||
| Nucleotide binding | 65 – 73 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 181 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 88 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 26 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 214 | 1 | Phosphothreonine Ref.6 | ||||||
Natural variations | |||||||||
| Alternative sequence | 628 – 752 | 125 | ADEPE…NDLEL → TLDPSKRQNSNRCVSGASLP QGSKIRSQTNLRESGDLRSQ VAIYLGIKRKPPPGCSDSPG V in isoform 2. | VSP_004946 | |||||
| Natural variant | 377 | 1 | R → Q: dbSNP rs35070611. Ref.7 | VAR_040766 | |||||
| Natural variant | 418 | 1 | R → C in a colorectal adenocarcinoma sample; somatic mutation. Ref.7 | VAR_040767 | |||||
Experimental info | |||||||||
| Sequence conflict | 70 | 1 | F → S in BAB39380. Ref.2 | ||||||
| Sequence conflict | 70 | 1 | F → S in BAC03375. Ref.2 | ||||||
| Sequence conflict | 518 | 1 | P → L in BAB55238. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "MARK4, homologue of MARK1, MARK2 and MARK3." Drewes G., Mandelkow E.M. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain. |
| [2] | "Isolation of a novel human gene, MARKL1, homologous to MARK3 and its involvement in hepatocellular carcinogenesis." Kato T., Satoh S., Okabe H., Kitahara O., Ono K., Kihara C., Tanaka T., Tsunoda T., Yamaoka Y., Nakamura Y., Furukawa Y. Neoplasia 3:4-9(2001) [PubMed: 11326310] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY. Tissue: Brain. |
| [3] | "Neural progenitor-restricted isoform of MARKL1 gene is upregulated by 19q13 amplification in human glioblastoma." Beghini A., Magnani I., Roversi G., Piepoli T., DiTerlizzi S., Pollo B., Conti A.M.F., Cowell J.K., Finocchiaro G., Larizza L. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain. |
| [4] | "Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O. DNA Res. 8:85-95(2001) [PubMed: 11347906] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain. |
| [5] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Thyroid. |
| [6] | "Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry." Wissing J., Jaensch L., Nimtz M., Dieterich G., Hornberger R., Keri G., Wehland J., Daub H. Mol. Cell. Proteomics 6:537-547(2007) [PubMed: 17192257] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26 AND THR-214, MASS SPECTROMETRY. |
| [7] | "Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. Stratton M.R.Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] GLN-377 AND CYS-418. |
Cross-references
Sequence databases | |
|---|---|
| AY057448 mRNA. Translation: AAL23683.1. AB049127 mRNA. Translation: BAB39380.1. AB088047 mRNA. Translation: BAC03375.1. AY120867 mRNA. Translation: AAM55491.1. AB058763 mRNA. Translation: BAB47489.1. Different initiation. AK027619 mRNA. Translation: BAB55238.1. Different initiation. AK075272 mRNA. Translation: BAC11510.1. | |
| IPI | IPI00064797. IPI00297959. |
| RefSeq | NP_113605.2. |
| UniGene | Hs.34314 |
3D structure databases | |
| SMR | Q96L34. Positions 53-370. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q96L34. 30 interactions. |
| STRING | Q96L34. |
PTM databases | |
| PhosphoSite | Q96L34. |
Proteomic databases | |
| PRIDE | Q96L34. |
Genome annotation databases | |
| Ensembl | ENST00000262891; ENSP00000262891; ENSG00000007047; Homo sapiens. [Genome view] |
| GeneID | 57787. |
| KEGG | hsa:57787. |
| UCSC | uc002pbb.1. human. |
Organism-specific databases | |
| CTD | 57787. |
| GeneCards | GC19P050446. |
| H-InvDB | HIX0015226. |
| HGNC | HGNC:13538. MARK4. |
| MIM | 606495. gene. |
| PharmGKB | PA30641. |
| HUGE | Search... |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | HBG315019. |
| HOVERGEN | Q96L34. |
| InParanoid | Q96L34. |
| OMA | TERPGSE. |
| OrthoDB | EOG9NP9PH. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.1. 247. |
Gene expression databases | |
| ArrayExpress | Q96L34. |
| Bgee | Q96L34. |
| CleanEx | HS_MARK4. |
| Genevestigator | Q96L34. |
| GermOnline | ENSG00000007047. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR020636. Ca/CaM-dep_prot_kinase-like. IPR001772. Kinase-assoc_KA1. IPR011009. Kinase-like_dom. IPR020670. MAP/microtub-affin-reg_kin2/4. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. IPR000449. UBA/transl_elong_EF1B_N. IPR015940. UBA/transl_elong_EF1B_N_euk. [Graphical view] |
| PANTHER | PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit. PTHR22982:SF55. MAP/microtub-affin-reg_kin2/4. 1 hit. |
| Pfam | PF02149. KA1. 1 hit. PF00069. Pkinase. 1 hit. PF00627. UBA. 1 hit. [Graphical view] |
| SMART | SM00220. S_TKc. 1 hit. SM00165. UBA. 1 hit. [Graphical view] |
| PROSITE | PS50032. KA1. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50030. UBA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 64721. |
| SOURCE | Search... |
Entry information
| Entry name | MARK4_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q96L34 Secondary accession number(s): Q8NG37 Q9BYD8 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 19 Human chromosome 19: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


