Q96L15 (NAR5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ecto-ADP-ribosyltransferase 5
EC=2.4.2.31

Alternative name(s):
Mono(ADP-ribosyl)transferase 5
NAD(P)(+)--arginine ADP-ribosyltransferase 5

Gene names

Name:	ART5
ORF Names:	UNQ575/PRO1137

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine. NADP+ + protein-L-arginine = nicotinamide + N(omega)-((2'-phospho-ADP)-D-ribosyl)-protein-L-arginine.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the Arg-specific ADP-ribosyltransferase family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Ligand	NAD NADP
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein ADP-ribosylation Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD(P)+-protein-arginine ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96L15-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96L15-2)

The sequence of this isoform differs from the canonical sequence as follows:
     195-262: Missing.
     275-291: ALGTGDLHMTKRHLQQP → VQLGSQSEGASSLPPWKTLLLAPGEFQLSGVGP

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 291	269	Ecto-ADP-ribosyltransferase 5		PRO_0000019333
Sites
	Active site	228	1	By similarity
	Binding site	99	1	NAD By similarity
	Binding site	160	1	NAD By similarity
	Binding site	180	1	NAD By similarity
	Binding site	214	1	NAD By similarity
Amino acid modifications
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
	Glycosylation	196	1	N-linked (GlcNAc...) Potential
	Glycosylation	250	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 258		By similarity
Natural variations
	Alternative sequence	195 – 262	68	Missing in isoform 2.		VSP_013145
	Alternative sequence	275 – 291	17	ALGTG…HLQQP → VQLGSQSEGASSLPPWKTLL LAPGEFQLSGVGP in isoform 2.		VSP_013146
	Natural variant	24	1	P → PT. Ref.1 Ref.2 Ref.4 Ref.5 Corresponds to variant rs72515796 [ dbSNP | Ensembl ].		VAR_063143
Experimental info
	Sequence conflict	43	1	A → V in AAH14577. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 4. Checksum: 9783A0473FB4613E	FASTA	291	32,054
	10 20 30 40 50 60 MALAALMIAL GSLGLHTWQA QAVPILPLGL APDTFDDTYV GCAEEMEEKA APLLKEEMAH 70 80 90 100 110 120 HALLRESWEA AQETWEDKRR GLTLPPGFKA QNGIAIMVYT NSSNTLYWEL NQAVRTGGGS 130 140 150 160 170 180 RELYMRHFPF KALHFYLIRA LQLLRGSGGC SRGPGEVVFR GVGSLRFEPK RLGDSVRLGQ 190 200 210 220 230 240 FASSSLDKAV AHRFGNATLF SLTTCFGAPI QAFSVFPKER EVLIPPHEVF LVTRFSQDGA 250 260 270 280 290 QSLVTLWSYN QTCSHFNCAY LGGEKRRGCV SAPGALGTGD LHMTKRHLQQ P
	Isoform 2 [UniParc]. Checksum: BA4A6D7AB29A73BD	FASTA	239	25,963
	10 20 30 40 50 60 MALAALMIAL GSLGLHTWQA QAVPILPLGL APDTFDDTYV GCAEEMEEKA APLLKEEMAH 70 80 90 100 110 120 HALLRESWEA AQETWEDKRR GLTLPPGFKA QNGIAIMVYT NSSNTLYWEL NQAVRTGGGS 130 140 150 160 170 180 RELYMRHFPF KALHFYLIRA LQLLRGSGGC SRGPGEVVFR GVGSLRFEPK RLGDSVRLGQ 190 200 210 220 230 FASSSLDKAV AHRFGEKRRG CVSAPGVQLG SQSEGASSLP PWKTLLLAPG EFQLSGVGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The family of toxin-related ecto-ADP-ribosyltransferases in humans and the mouse." Glowacki G., Braren R., Firner K., Nissen M., Kuehl M., Reche P., Bazan J.F., Cetkovic-Cvrlje M., Leiter E., Haag F., Koch-Nolte F. Protein Sci. 11:1657-1670(2002) [PubMed: 12070318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT THR-24 INS. Tissue: Testis.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT THR-24 INS.
[3]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT THR-24 INS.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-24 INS. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y16835 mRNA. Translation: CAC79987.1. AY358466 mRNA. Translation: AAQ88831.1. AC060812 Genomic DNA. No translation available. CH471158 Genomic DNA. Translation: EAX02556.1. BC014577 mRNA. Translation: AAH14577.1.
IPI	IPI00328943. IPI00432414.
RefSeq	NP_001073004.1. NM_001079536.1. NP_443750.2. NM_053017.3.
UniGene	Hs.125680.
3D structure databases
ProteinModelPortal	Q96L15.
SMR	Q96L15. Positions 26-260.
ModBase	Search...
Protein-protein interaction databases
STRING	Q96L15.
Polymorphism databases
DMDM	296439487.
Proteomic databases
PRIDE	Q96L15.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000359918; ENSP00000352992; ENSG00000167311. ENST00000397068; ENSP00000380258; ENSG00000167311.
GeneID	116969.
KEGG	hsa:116969.
UCSC	uc001lyd.2. human.
Organism-specific databases
CTD	116969.
GeneCards	GC11M003659.
HGNC	HGNC:24049. ART5.
MIM	610625. gene.
neXtProt	NX_Q96L15.
PharmGKB	PA134887713.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG04603.
GeneTree	ENSGT00530000062975.
HOGENOM	HBG443999.
HOVERGEN	HBG004464.
InParanoid	Q96L15.
OMA	LHFEPKR.
OrthoDB	EOG4V1719.
PhylomeDB	Q96L15.
Gene expression databases
ArrayExpress	Q96L15.
Bgee	Q96L15.
CleanEx	HS_ART5.
Genevestigator	Q96L15.
GermOnline	ENSG00000167311. Homo sapiens.
Family and domain databases
InterPro	IPR000768. ART. [Graphical view]
KO	K00775.
PANTHER	PTHR10339. ART. 1 hit.
Pfam	PF01129. ART. 1 hit. [Graphical view]
PRINTS	PR00970. RIBTRNSFRASE.
PROSITE	PS01291. ART. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	80046.
SOURCE	Search...

Entry information

Entry name

NAR5_HUMAN

Accession

Primary (citable) accession number: Q96L15
Secondary accession number(s): C9IYG7, Q6UX84, Q86W02

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 2002
Last sequence update:	May 18, 2010
Last modified:	January 25, 2012
This is version 84 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.