Reviewed, UniProtKB/Swiss-Prot Q96L15 (NAR5_HUMAN)

Names and origin

Protein names

Recommended name:
    Ecto-ADP-ribosyltransferase 5
    EC=2.4.2.31
Alternative name(s):
    NAD(P)(+)--arginine ADP-ribosyltransferase 5
    Mono(ADP-ribosyl)transferase 5

Gene names

Name:	ART5
ORF Names:	UNQ575/PRO1137

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine. NADP+ + protein-L-arginine = nicotinamide + N(omega)-((2'-phospho-ADP)-D-ribosyl)-protein-L-arginine.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the Arg-specific ADP-ribosyltransferase family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	NAD NADP
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid ADP-ribosylation Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD(P)+-protein-arginine ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96L15-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96L15-2)

The sequence of this isoform differs from the canonical sequence as follows:
     196-263: Missing.
     276-292: ALGTGDLHMTKRHLQQP → VQLGSQSEGASSLPPWKTLLLAPGEFQLSGVGP

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 292	270	Ecto-ADP-ribosyltransferase 5		PRO_0000019333
Sites
	Active site	229	1	By similarity
	Binding site	100	1	NAD By similarity
	Binding site	161	1	NAD By similarity
	Binding site	181	1	NAD By similarity
	Binding site	215	1	NAD By similarity
Amino acid modifications
	Glycosylation	102	1	N-linked (GlcNAc...) Potential
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
	Glycosylation	251	1	N-linked (GlcNAc...) Potential
	Disulfide bond	43 ↔ 259		By similarity
Natural variations
	Alternative sequence	196 – 263	68	Missing in isoform 2.		VSP_013145
	Alternative sequence	276 – 292	17	ALGTG…HLQQP → VQLGSQSEGASSLPPWKTLL LAPGEFQLSGVGP in isoform 2.		VSP_013146
Experimental info
	Sequence conflict	44	1	A → V in AAH14577. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: 7EF73CD03C657F57	FASTA	292	32,097
	10 20 30 40 50 60 MALAALMIAL GSLGLHTWQA QAVPTILPLG LAPDTFDDTY VGCAEEMAEK AAPLLKEEMA 70 80 90 100 110 120 HHALLRESWE AAQETWEDKR RGLTLPPGFK AQNGIAIMVY TNSSNTLYWE LNQAVRTGGG 130 140 150 160 170 180 SRELYMRHFP FKALHFYLIR ALQLLRGSGG CSRGPGEVVF RGVGSLRFEP KRLGDSVRLG 190 200 210 220 230 240 QFASSSLDKA VAHRFGNATL FSLTTCFGAP IQAFSVFPKE REVLIPPHEV FLVTRFSQDG 250 260 270 280 290 AQSLVTLWSY NQTCSHFNCA YLGGEKRRGC VSAPGALGTG DLHMTKRHLQ QP
	Isoform 2. Checksum: B659F9AB2FF75356	FASTA	240	26,006
	10 20 30 40 50 60 MALAALMIAL GSLGLHTWQA QAVPTILPLG LAPDTFDDTY VGCAEEMAEK AAPLLKEEMA 70 80 90 100 110 120 HHALLRESWE AAQETWEDKR RGLTLPPGFK AQNGIAIMVY TNSSNTLYWE LNQAVRTGGG 130 140 150 160 170 180 SRELYMRHFP FKALHFYLIR ALQLLRGSGG CSRGPGEVVF RGVGSLRFEP KRLGDSVRLG 190 200 210 220 230 240 QFASSSLDKA VAHRFGEKRR GCVSAPGVQL GSQSEGASSL PPWKTLLLAP GEFQLSGVGP

References

[1]

"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).

[2]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY358466 mRNA. Translation: AAQ88831.1. BC014577 mRNA. Translation: AAH14577.1.
IPI	IPI00328943. IPI00432414.
RefSeq	NP_443750.2.
UniGene	Hs.125680
3D structure databases
HSSP	HSSP built from PDB template 1GXY based on UniProtKB P20974.
ModBase	Search...
Proteomic databases
PRIDE	Q96L15.
Genome annotation databases
Ensembl	ENSG00000167311. Homo sapiens. [Contig view]
GeneID	116969.
Organism-specific databases
GeneCards	GC11M003616.
HGNC	HGNC:24049. ART5.
MIM	610625. gene.
PharmGKB	PA134887713.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q96L15.
HOVERGEN	Q96L15.
Enzyme and pathway databases
BRENDA	2.4.2.31. 247.
Gene expression databases
ArrayExpress	Q96L15.
Bgee	Q96L15.
CleanEx	HS_ART5.
GermOnline	ENSG00000167311. Homo sapiens.
Family and domain databases
InterPro	IPR000768. ART. [Graphical view]
PANTHER	PTHR10339. ART. 1 hit.
Pfam	PF01129. ART. 1 hit. [Graphical view]
PRINTS	PR00970. RIBTRNSFRASE.
PROSITE	PS01291. ART. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	80046.
SOURCE	Search...

Entry information

Entry name

NAR5_HUMAN

Accession

Primary (citable) accession number: Q96L15
Secondary accession number(s): Q6UX84

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 2002
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)