Q96KR4 (LMLN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leishmanolysin-like peptidase
EC=3.4.24.-

Alternative name(s):
Invadolysin

Gene names

Name:

LMLN

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	655 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Metalloprotease essential for the coordination of mitotic progression, and also plays a role in cell migration By similarity.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm. Lipid droplet. Note: Found in ring-like structures resembling invadopodia. In migrating cells it relocalizes from internal structures to the leading edge of cells. Ref.1 Ref.4
Tissue specificity	Expressed in all cell lines analyzed. Ref.1
Sequence similarities	Belongs to the peptidase M8 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Lipid droplet
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: InterPro cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA focal adhesion Inferred from direct assay. Source: HPA lipid particle Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96KR4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96KR4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: MVTTLGPKMA...CRHHVPSDTE → MGRRSGLLGLRPGPEPVALER
     411-411: G → GWYKANYSMAEKLDWGRGMGCDFVRKSCKFWIDQQRQK

Isoform 3 (identifier: Q96KR4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     411-411: G → GWYKANYSMAEKLDWGRGMGCDFVRKSCKFWIDQQRQK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 655	655	Leishmanolysin-like peptidase		PRO_0000303076
Sites
	Active site	273	1	By similarity
	Metal binding	272	1	Zinc; catalytic By similarity
	Metal binding	276	1	Zinc; catalytic By similarity
	Metal binding	378	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	308	1	N-linked (GlcNAc...) Potential
	Glycosylation	615	1	N-linked (GlcNAc...) Potential
	Disulfide bond	172 ↔ 235		By similarity
	Disulfide bond	358 ↔ 419		By similarity
	Disulfide bond	431 ↔ 525		By similarity
	Disulfide bond	555 ↔ 602		By similarity
Natural variations
	Alternative sequence	1 – 73	73	MVTTL…PSDTE → MGRRSGLLGLRPGPEPVALE R in isoform 2.		VSP_028002
	Alternative sequence	411	1	G → GWYKANYSMAEKLDWGRGMG CDFVRKSCKFWIDQQRQK in isoform 2 and isoform 3.		VSP_028003
	Natural variant	106	1	E → D. Corresponds to variant rs7373165 [ dbSNP | Ensembl ].		VAR_060158
Experimental info
	Sequence conflict	539	1	R → K in CAP49203. Ref.1
	Sequence conflict	539	1	R → K in CAP49204. Ref.1
	Sequence conflict	539	1	R → K in CAC42882. Ref.2
	Sequence conflict	539	1	R → K in CAC42883. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: D6A767CE3FA7A915	FASTA	655	73,568
	10 20 30 40 50 60 MVTTLGPKMA AEWGGGVGYS GSGPGRSRWR WSGSVWVRSV LLLLGGLRAS ATSTPVSLGS 70 80 90 100 110 120 SPPCRHHVPS DTEVINKVHL KANHVVKRDV DEHLRIKTVY DKSVEELLPE KKNLVKNKLF 130 140 150 160 170 180 PQAISYLEKT FQVRRPAGTI LLSRQCATNQ YLRKENDPHR YCTGECAAHT KCGPVIVPEE 190 200 210 220 230 240 HLQQCRVYRG GKWPHGAVGV PDQEGISDAD FVLYVGALAT ERCSHENIIS YAAYCQQEAN 250 260 270 280 290 300 MDRPIAGYAN LCPNMISTQP QEFVGMLSTV KHEVIHALGF SAGLFAFYHD KDGNPLTSRF 310 320 330 340 350 360 ADGLPPFNYS LGLYQWSDKV VRKVERLWDV RDNKIVRHTV YLLVTPRVVE EARKHFDCPV 370 380 390 400 410 420 LEGMELENQG GVGTELNHWE KRLLENEAMT GSHTQNRVLS RITLALMEDT GRQMLSPYCD 430 440 450 460 470 480 TLRSNPLQLT CRQDQRAVAV CNLQKFPKPL PQEYQYFDEL SGIPAEDLPY YGGSVEIADY 490 500 510 520 530 540 CPFSQEFSWH LSGEYQRSSD CRILENQPEI FKNYGAEKYG PHSVCLIQKS AFVMEKCERK 550 560 570 580 590 600 LSYPDWGSGC YQVSCSPQGL KVWVQDTSYL CSRAGQVLPV SIQMNGWIHD GNLLCPSCWD 610 620 630 640 650 FCELCPPETD PPATNLTRAL PLDLCSCSSS LVVTLWLLLG NLFPLLAGFL LCIWH
	Isoform 2 [UniParc]. Checksum: 272AB99BBE391B42	FASTA	640	72,780
	10 20 30 40 50 60 MGRRSGLLGL RPGPEPVALE RVINKVHLKA NHVVKRDVDE HLRIKTVYDK SVEELLPEKK 70 80 90 100 110 120 NLVKNKLFPQ AISYLEKTFQ VRRPAGTILL SRQCATNQYL RKENDPHRYC TGECAAHTKC 130 140 150 160 170 180 GPVIVPEEHL QQCRVYRGGK WPHGAVGVPD QEGISDADFV LYVGALATER CSHENIISYA 190 200 210 220 230 240 AYCQQEANMD RPIAGYANLC PNMISTQPQE FVGMLSTVKH EVIHALGFSA GLFAFYHDKD 250 260 270 280 290 300 GNPLTSRFAD GLPPFNYSLG LYQWSDKVVR KVERLWDVRD NKIVRHTVYL LVTPRVVEEA 310 320 330 340 350 360 RKHFDCPVLE GMELENQGGV GTELNHWEKR LLENEAMTGS HTQNRVLSRI TLALMEDTGW 370 380 390 400 410 420 YKANYSMAEK LDWGRGMGCD FVRKSCKFWI DQQRQKRQML SPYCDTLRSN PLQLTCRQDQ 430 440 450 460 470 480 RAVAVCNLQK FPKPLPQEYQ YFDELSGIPA EDLPYYGGSV EIADYCPFSQ EFSWHLSGEY 490 500 510 520 530 540 QRSSDCRILE NQPEIFKNYG AEKYGPHSVC LIQKSAFVME KCERKLSYPD WGSGCYQVSC 550 560 570 580 590 600 SPQGLKVWVQ DTSYLCSRAG QVLPVSIQMN GWIHDGNLLC PSCWDFCELC PPETDPPATN 610 620 630 640 LTRALPLDLC SCSSSLVVTL WLLLGNLFPL LAGFLLCIWH
	Isoform 3 [UniParc]. Checksum: 3000E53236452C1D	FASTA	692	78,111
	10 20 30 40 50 60 MVTTLGPKMA AEWGGGVGYS GSGPGRSRWR WSGSVWVRSV LLLLGGLRAS ATSTPVSLGS 70 80 90 100 110 120 SPPCRHHVPS DTEVINKVHL KANHVVKRDV DEHLRIKTVY DKSVEELLPE KKNLVKNKLF 130 140 150 160 170 180 PQAISYLEKT FQVRRPAGTI LLSRQCATNQ YLRKENDPHR YCTGECAAHT KCGPVIVPEE 190 200 210 220 230 240 HLQQCRVYRG GKWPHGAVGV PDQEGISDAD FVLYVGALAT ERCSHENIIS YAAYCQQEAN 250 260 270 280 290 300 MDRPIAGYAN LCPNMISTQP QEFVGMLSTV KHEVIHALGF SAGLFAFYHD KDGNPLTSRF 310 320 330 340 350 360 ADGLPPFNYS LGLYQWSDKV VRKVERLWDV RDNKIVRHTV YLLVTPRVVE EARKHFDCPV 370 380 390 400 410 420 LEGMELENQG GVGTELNHWE KRLLENEAMT GSHTQNRVLS RITLALMEDT GWYKANYSMA 430 440 450 460 470 480 EKLDWGRGMG CDFVRKSCKF WIDQQRQKRQ MLSPYCDTLR SNPLQLTCRQ DQRAVAVCNL 490 500 510 520 530 540 QKFPKPLPQE YQYFDELSGI PAEDLPYYGG SVEIADYCPF SQEFSWHLSG EYQRSSDCRI 550 560 570 580 590 600 LENQPEIFKN YGAEKYGPHS VCLIQKSAFV MEKCERKLSY PDWGSGCYQV SCSPQGLKVW 610 620 630 640 650 660 VQDTSYLCSR AGQVLPVSIQ MNGWIHDGNL LCPSCWDFCE LCPPETDPPA TNLTRALPLD 670 680 690 LCSCSSSLVV TLWLLLGNLF PLLAGFLLCI WH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The conserved metalloprotease invadolysin localizes to the surface of lipid droplets." Cobbe N., Marshall K.M., Gururaja Rao S., Chang C.W., Di Cara F., Duca E., Vass S., Kassan A., Heck M.M. J. Cell Sci. 122:3414-3423(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY. Tissue: Testis.
[2]	"Cloning and expression of splice variants of leishmanolysin-like protein, a human form of the protozoan leishmanolysin, EC 3.4.24.36." Chen J.M., Fortunato M., Barrett A.J. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Colon and Testis.
[3]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Invadolysin: a novel, conserved metalloprotease links mitotic structural rearrangements with cell migration." McHugh B., Krause S.A., Yu B., Deans A.M., Heasman S., McLaughlin P., Heck M.M. J. Cell Biol. 167:673-686(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM920777 mRNA. Translation: CAP49203.1. AM920778 mRNA. Translation: CAP49204.1. AJ312398 mRNA. Translation: CAC42882.1. AJ312399 mRNA. Translation: CAC42883.1. AC135893 Genomic DNA. No translation available.
IPI	IPI00064741. IPI00903090. IPI00924964.
RefSeq	NP_001129521.2. NM_001136049.2. NP_149018.2. NM_033029.3.
UniGene	Hs.518540.
3D structure databases
ProteinModelPortal	Q96KR4.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000410926.
Protein family/group databases
MEROPS	M08.003.
PTM databases
PhosphoSite	Q96KR4.
Polymorphism databases
DMDM	296434578.
Proteomic databases
PaxDb	Q96KR4.
PRIDE	Q96KR4.
Protocols and materials databases
DNASU	89782.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000330198; ENSP00000328829; ENSG00000185621. ENST00000420910; ENSP00000410926; ENSG00000185621. ENST00000482695; ENSP00000418324; ENSG00000185621.
GeneID	89782.
KEGG	hsa:89782.
UCSC	uc003fyt.3. human. uc011buo.2. human.
Organism-specific databases
CTD	89782.
GeneCards	GC03P197687.
HGNC	HGNC:15991. LMLN.
HPA	HPA016481. HPA028844.
MIM	609380. gene.
neXtProt	NX_Q96KR4.
PharmGKB	PA30402.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG279222.
HOGENOM	HOG000007103.
HOVERGEN	HBG087499.
InParanoid	Q96KR4.
KO	K13539.
OrthoDB	EOG4S7JPH.
Gene expression databases
ArrayExpress	Q96KR4.
Bgee	Q96KR4.
CleanEx	HS_LMLN.
Genevestigator	Q96KR4.
Family and domain databases
InterPro	IPR001577. Peptidase_M8. [Graphical view]
PANTHER	PTHR10942. PTHR10942. 1 hit.
Pfam	PF01457. Peptidase_M8. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	89782.
NextBio	35470729.
SOURCE	Search...

Entry information

Entry name

LMLN_HUMAN

Accession

Primary (citable) accession number: Q96KR4
Secondary accession number(s): B3LDG9 , B3LDH0, C9J796, F8WB28, Q96KR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.