Q96KR1 (ZFR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger RNA-binding protein
Short name=hZFR

Alternative name(s):
M-phase phosphoprotein homolog

Gene names

Name:

ZFR

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1074 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in postimplantation and gastrulation stages of development. Involved in the nucleocytoplasmic shuttling of STAU2. Binds to DNA and RNA By similarity.
Subunit structure	Found in a cytoplasmic RNP complex with STAU2. Interacts with STAU2. Does not interact with STAU1 By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Cytoplasmic granule By similarity. Chromosome By similarity. Note: Associated with chromosome foci in meiotic cells. Localizes in somatodendritic compartment of primary hippocampal neurons. Colocalizes with STAU2 in several cytosolic RNA granules By similarity.
Tissue specificity	Expressed in lung, liver, lymphocytes, heart, pancreas, placenta, brain and kidney. Ref.1
Sequence similarities	Contains 1 DZF domain.
Sequence caution	The sequence AAD40385.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH30540.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAI07418.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence BAB15147.1 differs from that shown. Reason: Erroneous initiation. The sequence CAC40818.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAC40818.1 differs from that shown. Reason: Frameshift at position 1057.

Ontologies

Keywords
Cellular component	Chromosome Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	DNA-binding RNA-binding
Molecular function	Developmental protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chromosome Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: HPA
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SMAD2	Q15796	2	EBI-2513582,EBI-1040141

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1074	1074	Zinc finger RNA-binding protein		PRO_0000312719
Regions
	Domain	790 – 1038	249	DZF
	Compositional bias	36 – 302	267	Ala-rich
Amino acid modifications
	Modified residue	509	1	N6-acetyllysine Ref.10
	Modified residue	1054	1	Phosphoserine Ref.7 Ref.9
Natural variations
	Natural variant	461	1	V → I. Ref.3 Corresponds to variant rs4867440 [ dbSNP | Ensembl ].		VAR_037554
	Natural variant	520	1	I → T. Ref.3 Corresponds to variant rs1051489 [ dbSNP | Ensembl ].		VAR_037555
Experimental info
	Sequence conflict	7	1	V → I in AAI07418. Ref.3
	Sequence conflict	643	1	E → L in AAD40385. Ref.4
	Sequence conflict	659	1	M → I in CAC40818. Ref.1
	Sequence conflict	794	1	L → F in AAD40385. Ref.4
	Sequence conflict	834	1	V → F in AAD40385. Ref.4
	Sequence conflict	913	1	Q → QE in CAC40818. Ref.1
	Sequence conflict	1016	1	F → K in CAC40818. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q96KR1 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 3E43654E7134E9FC	FASTA	1,074	117,012
	10 20 30 40 50 60 MIPICPVVSF TYVPSRLGED AKMATGNYFG FTHSGAAAAA AAAQYSQQPA SGVAYSHPTT 70 80 90 100 110 120 VASYTVHQAP VAAHTVTAAY APAAATVAVA RPAPVAVAAA ATAAAYGGYP TAHTATDYGY 130 140 150 160 170 180 TQRQQEAPPP PPPATTQNYQ DSYSYVRSTA PAVAYDSKQY YQQPTATAAA VAAAAQPQPS 190 200 210 220 230 240 VAETYYQTAP KAGYSQGATQ YTQAQQTRQV TAIKPATPSP ATTTFSIYPV SSTVQPVAAA 250 260 270 280 290 300 ATVVPSYTQS ATYSTTAVTY SGTSYSGYEA AVYSAASSYY QQQQQQQKQA AAAAAAAAAT 310 320 330 340 350 360 AAWTGTTFTK KAPFQNKQLK PKQPPKPPQI HYCDVCKISC AGPQTYKEHL EGQKHKKKEA 370 380 390 400 410 420 ALKASQNTSS SNSSTRGTQN QLRCELCDVS CTGADAYAAH IRGAKHQKVV KLHTKLGKPI 430 440 450 460 470 480 PSTEPNVVSQ ATSSTAVSAS KPTASPSSIA ANNCTVNTSS VATSSMKGLT TTGNSSLNST 490 500 510 520 530 540 SNTKVSAVPT NMAAKKTSTP KINFVGGNKL QSTGNKAEDI KGTECVKSTP VTSAVQIPEV 550 560 570 580 590 600 KQDTVSEPVT PASLAALQSD VQPVGHDYVE EVRNDEGKVI RFHCKLCECS FNDPNAKEMH 610 620 630 640 650 660 LKGRRHRLQY KKKVNPDLQV EVKPSIRARK IQEEKMRKQM QKEEYWRRRE EEERWRMEMR 670 680 690 700 710 720 RYEEDMYWRR MEEEQHHWDD RRRMPDGGYP HGPPGPLGLL GVRPGMPPQP QGPAPLRRPD 730 740 750 760 770 780 SSDDRYVMTK HATIYPTEEE LQAVQKIVSI TERALKLVSD SLSEHEKNKN KEGDDKKEGG 790 800 810 820 830 840 KDRALKGVLR VGVLAKGLLL RGDRNVNLVL LCSEKPSKTL LSRIAENLPK QLAVISPEKY 850 860 870 880 890 900 DIKCAVSEAA IILNSCVEPK MQVTITLTSP IIREENMREG DVTSGMVKDP PDVLDRQKCL 910 920 930 940 950 960 DALAALRHAK WFQARANGLQ SCVIIIRILR DLCQRVPTWS DFPSWAMELL VEKAISSASS 970 980 990 1000 1010 1020 PQSPGDALRR VFECISSGII LKGSPGLLDP CEKDPFDTLA TMTDQQREDI TSSAQFALRL 1030 1040 1050 1060 1070 LAFRQIHKVL GMDPLPQMSQ RFNIHNNRKR RRDSDGVDGF EAEGKKDKKD YDNF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of the human single copy homologue of the mouse zinc finger protein zfr." Kleines M., Gaertner A., Ritter K., Schaade L. Gene 275:157-162(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ILE-461 AND THR-520. Tissue: Bone marrow, Duodenum, Eye, Lung and Uterus.
[4]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 643-1074. Tissue: Pituitary.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 744-1074. Tissue: Hepatoma.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 970-1074. Tissue: Testis.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1054, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1054, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-509, MASS SPECTROMETRY.
[11]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ314790 mRNA. Translation: CAC40818.1. Sequence problems. AC008949 Genomic DNA. No translation available. BC030540 mRNA. Translation: AAH30540.1. Sequence problems. BC051893 mRNA. Translation: AAH51893.1. BC062986 mRNA. Translation: AAH62986.1. BC107417 mRNA. Translation: AAI07418.1. Sequence problems. BC137084 mRNA. Translation: AAI37085.1. AF100742 mRNA. Translation: AAD40385.1. Different initiation. AK025481 mRNA. Translation: BAB15147.1. Different initiation. AL137258 mRNA. Translation: CAB70659.1.
IPI	IPI00784224.
PIR	T46329.
RefSeq	NP_057191.2. NM_016107.3.
UniGene	Hs.435231.
3D structure databases
ProteinModelPortal	Q96KR1.
SMR	Q96KR1. Positions 722-1039.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47293N.
IntAct	Q96KR1. 10 interactions.
PTM databases
PhosphoSite	Q96KR1.
Polymorphism databases
DMDM	162416228.
Proteomic databases
PaxDb	Q96KR1.
PRIDE	Q96KR1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000265069; ENSP00000265069; ENSG00000056097.
GeneID	51663.
KEGG	hsa:51663.
UCSC	uc003jhr.1. human.
Organism-specific databases
CTD	51663.
GeneCards	GC05M032390.
H-InvDB	HIX0004787.
HGNC	HGNC:17277. ZFR.
HPA	HPA016666.
neXtProt	NX_Q96KR1.
PharmGKB	PA38219.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG292385.
HOVERGEN	HBG108765.
InParanoid	Q96KR1.
KO	K13203.
OMA	EDAKMAT.
OrthoDB	EOG4HX511.
Gene expression databases
ArrayExpress	Q96KR1.
Bgee	Q96KR1.
CleanEx	HS_ZFR.
Genevestigator	Q96KR1.
Family and domain databases
InterPro	IPR006561. DZF. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR003604. Znf_U1. [Graphical view]
Pfam	PF07528. DZF. 1 hit. [Graphical view]
SMART	SM00572. DZF. 1 hit. SM00355. ZnF_C2H2. 3 hits. SM00451. ZnF_U1. 3 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 3 hits. Uncertain. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ZFR. human.
GenomeRNAi	51663.
NextBio	55646.

Entry information

Entry name

ZFR_HUMAN

Accession

Primary (citable) accession number: Q96KR1
Secondary accession number(s): B2RNR5 , Q05C08, Q3B7X5, Q6P5A3, Q86UA0, Q9H6V4, Q9NTI1, Q9Y687

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.