Q96KQ4 (ASPP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Apoptosis-stimulating of p53 protein 1

Alternative name(s):
Protein phosphatase 1 regulatory subunit 13B

Gene names

Name:	PPP1R13B
Synonyms:	ASPP1, KIAA0771

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1090 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Ref.1
Subunit structure	Interacts with TP53. Ref.1
Subcellular location	Cytoplasm. Nucleus. Note: Predominantly cytoplasmic. Some fraction is nuclear. Ref.1
Tissue specificity	Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Ref.1
Domain	The ankyrin repeats and the SH3 domain are required for specific interactions with TP53.
Miscellaneous	In contrast to its official gene name, it is not a regulatory subunit of protein phosphatase 1. This name was given due to its similarity with a protein that binds to protein phosphatase 1.
Sequence similarities	Belongs to the ASPP family. Contains 2 ANK repeats. Contains 1 SH3 domain.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm Nucleus
Domain	ANK repeat Repeat SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW induction of apoptosis Inferred from direct assay Ref.1. Source: UniProtKB negative regulation of cell cycle Traceable author statement PubMed 14729977. Source: UniProtKB
Cellular_component	cytoplasm Traceable author statement PubMed 15782125. Source: UniProtKB nucleus Inferred from direct assay Ref.1. Source: UniProtKB plasma membrane Inferred from direct assay. Source: HPA
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1090	1090	Apoptosis-stimulating of p53 protein 1		PRO_0000066962
Regions
	Repeat	920 – 952	33	ANK 1
	Repeat	953 – 985	33	ANK 2
	Domain	1019 – 1081	63	SH3
	Compositional bias	132 – 292	161	Gln-rich
	Compositional bias	428 – 860	433	Pro-rich
Amino acid modifications
	Modified residue	332	1	Phosphoserine Ref.7
	Modified residue	335	1	Phosphoserine Ref.7
	Modified residue	671	1	Phosphoserine By similarity
	Modified residue	681	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	68	1	K → I in CAC83011. Ref.1
	Sequence conflict	112	1	E → D in CAC83011. Ref.1
	Sequence conflict	117	1	N → Y in CAC83011. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q96KQ4 [UniParc]. Last modified May 18, 2010. Version 3. Checksum: 0026F07D7AB33295	FASTA	1,090	119,565
	10 20 30 40 50 60 MMPMILTVFL SNNEQILTEV PITPETTCRD VVEFCKEPGE GSCHLAEVWR GNERPIPFDH 70 80 90 100 110 120 MMYEHLQKWG PRREEVKFFL RHEDSPTENS EQGGRQTQEQ RTQRNVINVP GEKRTENGVG 130 140 150 160 170 180 NPRVELTLSE LQDMAARQQQ QIENQQQMLV AKEQRLHFLK QQERRQQQSI SENEKLQKLK 190 200 210 220 230 240 ERVEAQENKL KKIRAMRGQV DYSKIMNGNL SAEIERFSAM FQEKKQEVQT AILRVDQLSQ 250 260 270 280 290 300 QLEDLKKGKL NGFQSYNGKL TGPAAVELKR LYQELQIRNQ LNQEQNSKLQ QQKELLNKRN 310 320 330 340 350 360 MEVAMMDKRI SELRERLYGK KIQLNRVNGT SSPQSPLSTS GRVAAVGPYI QVPSAGSFPV 370 380 390 400 410 420 LGDPIKPQSL SIASNAAHGR SKSANDGNWP TLKQNSSSSV KPVQVAGADW KDPSVEGSVK 430 440 450 460 470 480 QGTVSSQPVP FSALGPTEKP GIEIGKVPPP IPGVGKQLPP SYGTYPSPTP LGPGSTSSLE 490 500 510 520 530 540 RRKEGSLPRP SAGLPSRQRP TLLPATGSTP QPGSSQQIQQ RISVPPSPTY PPAGPPAFPA 550 560 570 580 590 600 GDSKPELPLT VAIRPFLADK GSRPQSPRKG PQTVNSSSIY SMYLQQATPP KNYQPAAHSA 610 620 630 640 650 660 LNKSVKAVYG KPVLPSGSTS PSPLPFLHGS LSTGTPQPQP PSESTEKEPE QDGPAAPADG 670 680 690 700 710 720 STVESLPRPL SPTKLTPIVH SPLRYQSDAD LEALRRKLAN APRPLKKRSS ITEPEGPGGP 730 740 750 760 770 780 NIQKLLYQRF NTLAGGMEGT PFYQPSPSQD FMGTLADVDN GNTNANGNLE ELPPAQPTAP 790 800 810 820 830 840 LPAEPAPSSD ANDNELPSPE PEELICPQTT HQTAEPAEDN NNNVATVPTT EQIPSPVAEA 850 860 870 880 890 900 PSPGEEQVPP APLPPASHPP ATSTNKRTNL KKPNSERTGH GLRVRFNPLA LLLDASLEGE 910 920 930 940 950 960 FDLVQRIIYE VEDPSKPNDE GITPLHNAVC AGHHHIVKFL LDFGVNVNAA DSDGWTPLHC 970 980 990 1000 1010 1020 AASCNSVHLC KQLVESGAAI FASTISDIET AADKCEEMEE GYIQCSQFLY GVQEKLGVMN 1030 1040 1050 1060 1070 1080 KGVAYALWDY EAQNSDELSF HEGDALTILR RKDESETEWW WARLGDREGY VPKNLLGLYP 1090 RIKPRQRTLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ASPP proteins specifically stimulate the apoptotic function of p53." Samuels-Lev Y., O'Connor D.J., Bergamaschi D., Trigiante G., Hsieh J.-K., Zhong S., Campargue I., Naumovski L., Crook T., Lu X. Mol. Cell 8:781-794(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH TP53.
[2]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 143-1090. Tissue: Brain.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-681, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332 AND SER-335, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ318887 mRNA. Translation: CAC83011.2. AL049840 Genomic DNA. No translation available. CH471061 Genomic DNA. Translation: EAW81847.1. BC136527 mRNA. Translation: AAI36528.1. AB018314 mRNA. Translation: BAA34491.1.
IPI	IPI00306903.
RefSeq	NP_056131.2. NM_015316.2.
UniGene	Hs.709297.
3D structure databases
ProteinModelPortal	Q96KQ4.
SMR	Q96KQ4. Positions 1-83, 887-1083.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96KQ4. 9 interactions.
MINT	MINT-1130841.
STRING	9606.ENSP00000202556.
PTM databases
PhosphoSite	Q96KQ4.
Polymorphism databases
DMDM	296439434.
Proteomic databases
PaxDb	Q96KQ4.
PRIDE	Q96KQ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000202556; ENSP00000202556; ENSG00000088808.
GeneID	23368.
KEGG	hsa:23368.
UCSC	uc001yof.1. human.
Organism-specific databases
CTD	23368.
GeneCards	GC14M104200.
H-InvDB	HIX0012009.
HGNC	HGNC:14950. PPP1R13B.
HPA	CAB015454. HPA006394.
MIM	606455. gene.
neXtProt	NX_Q96KQ4.
PharmGKB	PA33622.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG283717.
HOGENOM	HOG000034106.
HOVERGEN	HBG050596.
InParanoid	Q96KQ4.
OMA	EPAEDNN.
OrthoDB	EOG4RNB7N.
PhylomeDB	Q96KQ4.
Gene expression databases
ArrayExpress	Q96KQ4.
Bgee	Q96KQ4.
CleanEx	HS_PPP1R13B.
Genevestigator	Q96KQ4.
GermOnline	ENSG00000088808. Homo sapiens.
Family and domain databases
Gene3D	1.25.40.20. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR001452. SH3_domain. [Graphical view]
Pfam	PF00023. Ank. 2 hits. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00452. SH3DOMAIN.
SMART	SM00248. ANK. 2 hits. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 2 hits. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PPP1R13B. human.
GenomeRNAi	23368.
NextBio	45436.
SOURCE	Search...

Entry information

Entry name

ASPP1_HUMAN

Accession

Primary (citable) accession number: Q96KQ4
Secondary accession number(s): B2RMX5, O94870

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	May 18, 2010
Last modified:	April 3, 2013
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.