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Q96KQ4 (ASPP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Apoptosis-stimulating of p53 protein 1
Alternative name(s):
Protein phosphatase 1 regulatory subunit 13B
Gene names
Name:PPP1R13B
Synonyms:ASPP1, KIAA0771
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1090 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Ref.1

Subunit structure

Interacts with TP53. Ref.1

Subcellular location

Cytoplasm. Nucleus. Note: Predominantly cytoplasmic. Some fraction is nuclear. Ref.1

Tissue specificity

Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Ref.1

Domain

The ankyrin repeats and the SH3 domain are required for specific interactions with TP53.

Miscellaneous

In contrast to its official gene name, it is not a regulatory subunit of protein phosphatase 1. This name was given due to its similarity with a protein that binds to protein phosphatase 1.

Sequence similarities

Belongs to the ASPP family.

Contains 2 ANK repeats.

Contains 1 SH3 domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

EP300Q094722EBI-1105153,EBI-447295
PPP1CAP621365EBI-1105153,EBI-357253

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10901090Apoptosis-stimulating of p53 protein 1
PRO_0000066962

Regions

Repeat920 – 95233ANK 1
Repeat953 – 98533ANK 2
Domain1019 – 108163SH3
Compositional bias132 – 292161Gln-rich
Compositional bias428 – 860433Pro-rich

Amino acid modifications

Modified residue3321Phosphoserine Ref.7
Modified residue3351Phosphoserine Ref.7
Modified residue6811Phosphoserine Ref.6

Experimental info

Sequence conflict681K → I in CAC83011. Ref.1
Sequence conflict1121E → D in CAC83011. Ref.1
Sequence conflict1171N → Y in CAC83011. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q96KQ4 [UniParc].

Last modified May 18, 2010. Version 3.
Checksum: 0026F07D7AB33295

FASTA1,090119,565
        10         20         30         40         50         60 
MMPMILTVFL SNNEQILTEV PITPETTCRD VVEFCKEPGE GSCHLAEVWR GNERPIPFDH 

        70         80         90        100        110        120 
MMYEHLQKWG PRREEVKFFL RHEDSPTENS EQGGRQTQEQ RTQRNVINVP GEKRTENGVG 

       130        140        150        160        170        180 
NPRVELTLSE LQDMAARQQQ QIENQQQMLV AKEQRLHFLK QQERRQQQSI SENEKLQKLK 

       190        200        210        220        230        240 
ERVEAQENKL KKIRAMRGQV DYSKIMNGNL SAEIERFSAM FQEKKQEVQT AILRVDQLSQ 

       250        260        270        280        290        300 
QLEDLKKGKL NGFQSYNGKL TGPAAVELKR LYQELQIRNQ LNQEQNSKLQ QQKELLNKRN 

       310        320        330        340        350        360 
MEVAMMDKRI SELRERLYGK KIQLNRVNGT SSPQSPLSTS GRVAAVGPYI QVPSAGSFPV 

       370        380        390        400        410        420 
LGDPIKPQSL SIASNAAHGR SKSANDGNWP TLKQNSSSSV KPVQVAGADW KDPSVEGSVK 

       430        440        450        460        470        480 
QGTVSSQPVP FSALGPTEKP GIEIGKVPPP IPGVGKQLPP SYGTYPSPTP LGPGSTSSLE 

       490        500        510        520        530        540 
RRKEGSLPRP SAGLPSRQRP TLLPATGSTP QPGSSQQIQQ RISVPPSPTY PPAGPPAFPA 

       550        560        570        580        590        600 
GDSKPELPLT VAIRPFLADK GSRPQSPRKG PQTVNSSSIY SMYLQQATPP KNYQPAAHSA 

       610        620        630        640        650        660 
LNKSVKAVYG KPVLPSGSTS PSPLPFLHGS LSTGTPQPQP PSESTEKEPE QDGPAAPADG 

       670        680        690        700        710        720 
STVESLPRPL SPTKLTPIVH SPLRYQSDAD LEALRRKLAN APRPLKKRSS ITEPEGPGGP 

       730        740        750        760        770        780 
NIQKLLYQRF NTLAGGMEGT PFYQPSPSQD FMGTLADVDN GNTNANGNLE ELPPAQPTAP 

       790        800        810        820        830        840 
LPAEPAPSSD ANDNELPSPE PEELICPQTT HQTAEPAEDN NNNVATVPTT EQIPSPVAEA 

       850        860        870        880        890        900 
PSPGEEQVPP APLPPASHPP ATSTNKRTNL KKPNSERTGH GLRVRFNPLA LLLDASLEGE 

       910        920        930        940        950        960 
FDLVQRIIYE VEDPSKPNDE GITPLHNAVC AGHHHIVKFL LDFGVNVNAA DSDGWTPLHC 

       970        980        990       1000       1010       1020 
AASCNSVHLC KQLVESGAAI FASTISDIET AADKCEEMEE GYIQCSQFLY GVQEKLGVMN 

      1030       1040       1050       1060       1070       1080 
KGVAYALWDY EAQNSDELSF HEGDALTILR RKDESETEWW WARLGDREGY VPKNLLGLYP 

      1090 
RIKPRQRTLA 

« Hide

References

« Hide 'large scale' references
[1]"ASPP proteins specifically stimulate the apoptotic function of p53."
Samuels-Lev Y., O'Connor D.J., Bergamaschi D., Trigiante G., Hsieh J.-K., Zhong S., Campargue I., Naumovski L., Crook T., Lu X.
Mol. Cell 8:781-794(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH TP53.
[2]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 143-1090.
Tissue: Brain.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-681, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332 AND SER-335, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ318887 mRNA. Translation: CAC83011.2.
AL049840 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW81847.1.
BC136527 mRNA. Translation: AAI36528.1.
AB018314 mRNA. Translation: BAA34491.1.
CCDSCCDS41997.1.
RefSeqNP_056131.2. NM_015316.2.
UniGeneHs.709297.

3D structure databases

ProteinModelPortalQ96KQ4.
SMRQ96KQ4. Positions 1-83, 887-1083.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116948. 14 interactions.
IntActQ96KQ4. 22 interactions.
MINTMINT-1130841.
STRING9606.ENSP00000202556.

PTM databases

PhosphoSiteQ96KQ4.

Polymorphism databases

DMDM296439434.

Proteomic databases

MaxQBQ96KQ4.
PaxDbQ96KQ4.
PRIDEQ96KQ4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000202556; ENSP00000202556; ENSG00000088808.
GeneID23368.
KEGGhsa:23368.
UCSCuc001yof.1. human.

Organism-specific databases

CTD23368.
GeneCardsGC14M104200.
H-InvDBHIX0012009.
HGNCHGNC:14950. PPP1R13B.
HPACAB015454.
HPA006394.
MIM606455. gene.
neXtProtNX_Q96KQ4.
PharmGKBPA33622.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG283717.
HOGENOMHOG000034106.
HOVERGENHBG050596.
InParanoidQ96KQ4.
KOK17554.
OMAKLTPMVH.
OrthoDBEOG73FQKX.
PhylomeDBQ96KQ4.
TreeFamTF105545.

Gene expression databases

ArrayExpressQ96KQ4.
BgeeQ96KQ4.
CleanExHS_PPP1R13B.
GenevestigatorQ96KQ4.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR028319. ASPP_1.
IPR001452. SH3_domain.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERPTHR24131:SF5. PTHR24131:SF5. 1 hit.
PfamPF00023. Ank. 2 hits.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSPR00452. SH3DOMAIN.
SMARTSM00248. ANK. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPPP1R13B. human.
GeneWikiPPP1R13B.
GenomeRNAi23368.
NextBio45436.
PROQ96KQ4.
SOURCESearch...

Entry information

Entry nameASPP1_HUMAN
AccessionPrimary (citable) accession number: Q96KQ4
Secondary accession number(s): B2RMX5, O94870
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM