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Q96KN2

- CNDP1_HUMAN

UniProt

Q96KN2 - CNDP1_HUMAN

Protein

Beta-Ala-His dipeptidase

Gene

CNDP1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 4 (11 Jan 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Preferential hydrolysis of the beta-Ala-|-His dipeptide (carnosine), and also anserine, Xaa-|-His dipeptides and other dipeptides including homocarnosine.2 Publications

    Cofactori

    Binds 2 zinc ions per subunit.1 Publication

    Enzyme regulationi

    Inhibited by the metal chelator 1,10-o-phenantrolin. The inhibitory concentration 50% (IC50) is 5 µM.

    Kineticsi

    1 hour incubation in 50 mM Tris-HCl, pH 7.5.

    1. KM=1.27 µM for carnosine (at 30 degrees Celsius and in the absence of cadmium ions)1 Publication
    2. KM=11.00 µM for carnosine (at 30 degrees Celsius and in the presence of 200 µM cadmium ions)1 Publication
    3. KM=0.20 µM for homocarnosine (at 30 degrees Celsius and in the absence of cadmium ions)1 Publication
    4. KM=1.0 µM for homocarnosine (at 30 degrees Celsius and in the presence of 200 µM cadmium ions)1 Publication

    pH dependencei

    Optimum pH is 8.5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi132 – 1321Zinc 2
    Active sitei134 – 1341By similarity
    Metal bindingi165 – 1651Zinc 1
    Metal bindingi165 – 1651Zinc 2
    Active sitei199 – 1991Proton acceptorBy similarity
    Metal bindingi200 – 2001Zinc 1
    Metal bindingi228 – 2281Zinc 2
    Metal bindingi478 – 4781Zinc 1

    GO - Molecular functioni

    1. carboxypeptidase activity Source: UniProtKB-KW
    2. dipeptidase activity Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. metallopeptidase activity Source: UniProtKB-KW
    5. tripeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Carboxypeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:HS07681-MONOMER.
    SABIO-RKQ96KN2.

    Protein family/group databases

    MEROPSiM20.006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-Ala-His dipeptidase (EC:3.4.13.20)
    Alternative name(s):
    CNDP dipeptidase 1
    Carnosine dipeptidase 1
    Glutamate carboxypeptidase-like protein 2
    Serum carnosinase
    Gene namesi
    Name:CNDP1
    Synonyms:CN1, CPGL2
    ORF Names:UNQ1915/PRO4380
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 18

    Organism-specific databases

    HGNCiHGNC:20675. CNDP1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi132 – 1321H → A: Loss of activity. 1 Publication
    Mutagenesisi165 – 1651D → A: Loss of activity. 1 Publication
    Mutagenesisi200 – 2001E → A: Loss of activity. 1 Publication

    Organism-specific databases

    PharmGKBiPA134907547.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 507481Beta-Ala-His dipeptidasePRO_0000026809Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei219 – 2191PhosphoserineBy similarity
    Glycosylationi322 – 3221N-linked (GlcNAc...)1 Publication
    Glycosylationi382 – 3821N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ96KN2.
    PeptideAtlasiQ96KN2.
    PRIDEiQ96KN2.

    PTM databases

    PhosphoSiteiQ96KN2.

    Expressioni

    Tissue specificityi

    Found in serum and adult nervous central system. Absent in serum from patients with homocarnosinosis.2 Publications

    Gene expression databases

    ArrayExpressiQ96KN2.
    BgeeiQ96KN2.
    CleanExiHS_CNDP1.
    GenevestigatoriQ96KN2.

    Organism-specific databases

    HPAiHPA008933.

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Protein-protein interaction databases

    BioGridi124230. 1 interaction.
    STRINGi9606.ENSP00000351682.

    Structurei

    Secondary structure

    1
    507
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi35 – 4410
    Helixi46 – 5712
    Beta strandi62 – 654
    Helixi68 – 8720
    Beta strandi91 – 955
    Beta strandi99 – 1013
    Beta strandi107 – 1093
    Beta strandi113 – 1186
    Beta strandi126 – 1327
    Helixi140 – 1423
    Beta strandi152 – 1543
    Beta strandi157 – 1604
    Turni161 – 1666
    Helixi167 – 18216
    Beta strandi188 – 19710
    Helixi199 – 2013
    Turni202 – 2054
    Helixi206 – 2138
    Turni214 – 2174
    Beta strandi223 – 2264
    Beta strandi238 – 2436
    Beta strandi245 – 25511
    Turni262 – 2643
    Helixi272 – 2809
    Turni293 – 2986
    Helixi304 – 3118
    Helixi317 – 3248
    Helixi334 – 3429
    Beta strandi346 – 35510
    Beta strandi358 – 3603
    Beta strandi367 – 37711
    Helixi383 – 39917
    Beta strandi404 – 41512
    Helixi425 – 43814
    Beta strandi443 – 4497
    Helixi452 – 4609
    Beta strandi483 – 4853
    Helixi486 – 50419

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3DLJX-ray2.26A/B27-507[»]
    ProteinModelPortaliQ96KN2.
    SMRiQ96KN2. Positions 30-506.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ96KN2.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M20A family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0624.
    HOGENOMiHOG000216709.
    HOVERGENiHBG051103.
    InParanoidiQ96KN2.
    KOiK05604.
    OMAiWINAVSA.
    OrthoDBiEOG7JHM55.
    PhylomeDBiQ96KN2.
    TreeFamiTF300633.

    Family and domain databases

    Gene3Di3.30.70.360. 1 hit.
    InterProiIPR001261. ArgE/DapE_CS.
    IPR017153. GSH_degradosome_DUG1.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view]
    PfamiPF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037242. CNDP_dipeptidase. 1 hit.
    PROSITEiPS00759. ARGE_DAPE_CPG2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q96KN2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPKLGRMAA SLLAVLLLLL ERGMFSSPSP PPALLEKVFQ YIDLHQDEFV    50
    QTLKEWVAIE SDSVQPVPRF RQELFRMMAV AADTLQRLGA RVASVDMGPQ 100
    QLPDGQSLPI PPIILAELGS DPTKGTVCFY GHLDVQPADR GDGWLTDPYV 150
    LTEVDGKLYG RGATDNKGPV LAWINAVSAF RALEQDLPVN IKFIIEGMEE 200
    AGSVALEELV EKEKDRFFSG VDYIVISDNL WISQRKPAIT YGTRGNSYFM 250
    VEVKCRDQDF HSGTFGGILH EPMADLVALL GSLVDSSGHI LVPGIYDEVV 300
    PLTEEEINTY KAIHLDLEEY RNSSRVEKFL FDTKEEILMH LWRYPSLSIH 350
    GIEGAFDEPG TKTVIPGRVI GKFSIRLVPH MNVSAVEKQV TRHLEDVFSK 400
    RNSSNKMVVS MTLGLHPWIA NIDDTQYLAA KRAIRTVFGT EPDMIRDGST 450
    IPIAKMFQEI VHKSVVLIPL GAVDDGEHSQ NEKINRWNYI EGTKLFAAFF 500
    LEMAQLH 507
    Length:507
    Mass (Da):56,706
    Last modified:January 11, 2011 - v4
    Checksum:i756CCD872996F192
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti155 – 1551D → G in CAD10388. 1 PublicationCurated
    Sequence conflicti155 – 1551D → G in AAI10296. (PubMed:15489334)Curated
    Sequence conflicti237 – 2371P → L in CAD10388. 1 PublicationCurated
    Sequence conflicti272 – 2721P → L in CAD10388. 1 PublicationCurated

    Polymorphismi

    The number of trinucleotide (CTG) repeat varies among different alleles leading to insertion of Leu residues in the signal peptide. The allele with 5 leucines (as shown in the reference entry) is known as the Mannheim allele. Diabetic patients with the CNDP1 Mannheim allele are less susceptible for nephropathy.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti6 – 61G → R.
    Corresponds to variant rs11151964 [ dbSNP | Ensembl ].
    VAR_027147
    Natural varianti20 – 201L → LL.2 Publications
    VAR_027148
    Natural varianti113 – 1131I → V.4 Publications
    Corresponds to variant rs4263028 [ dbSNP | Ensembl ].
    VAR_027149

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ417564 mRNA. Translation: CAD10388.1.
    AY358756 mRNA. Translation: AAQ89116.1.
    BC004271 mRNA. No translation available.
    BC110295 mRNA. Translation: AAI10296.1.
    BC113512 mRNA. Translation: AAI13513.1.
    BC117122 mRNA. Translation: AAI17123.1.
    CCDSiCCDS12007.1.
    RefSeqiNP_116038.4. NM_032649.5.
    UniGeneiHs.400613.

    Genome annotation databases

    EnsembliENST00000358821; ENSP00000351682; ENSG00000150656.
    GeneIDi84735.
    KEGGihsa:84735.
    UCSCiuc002llq.3. human.

    Polymorphism databases

    DMDMi317373563.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ417564 mRNA. Translation: CAD10388.1 .
    AY358756 mRNA. Translation: AAQ89116.1 .
    BC004271 mRNA. No translation available.
    BC110295 mRNA. Translation: AAI10296.1 .
    BC113512 mRNA. Translation: AAI13513.1 .
    BC117122 mRNA. Translation: AAI17123.1 .
    CCDSi CCDS12007.1.
    RefSeqi NP_116038.4. NM_032649.5.
    UniGenei Hs.400613.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3DLJ X-ray 2.26 A/B 27-507 [» ]
    ProteinModelPortali Q96KN2.
    SMRi Q96KN2. Positions 30-506.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 124230. 1 interaction.
    STRINGi 9606.ENSP00000351682.

    Protein family/group databases

    MEROPSi M20.006.

    PTM databases

    PhosphoSitei Q96KN2.

    Polymorphism databases

    DMDMi 317373563.

    Proteomic databases

    PaxDbi Q96KN2.
    PeptideAtlasi Q96KN2.
    PRIDEi Q96KN2.

    Protocols and materials databases

    DNASUi 84735.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000358821 ; ENSP00000351682 ; ENSG00000150656 .
    GeneIDi 84735.
    KEGGi hsa:84735.
    UCSCi uc002llq.3. human.

    Organism-specific databases

    CTDi 84735.
    GeneCardsi GC18P072201.
    HGNCi HGNC:20675. CNDP1.
    HPAi HPA008933.
    MIMi 609064. gene.
    neXtProti NX_Q96KN2.
    PharmGKBi PA134907547.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0624.
    HOGENOMi HOG000216709.
    HOVERGENi HBG051103.
    InParanoidi Q96KN2.
    KOi K05604.
    OMAi WINAVSA.
    OrthoDBi EOG7JHM55.
    PhylomeDBi Q96KN2.
    TreeFami TF300633.

    Enzyme and pathway databases

    BioCyci MetaCyc:HS07681-MONOMER.
    SABIO-RK Q96KN2.

    Miscellaneous databases

    EvolutionaryTracei Q96KN2.
    GeneWikii CNDP1.
    GenomeRNAii 84735.
    NextBioi 74860.
    PROi Q96KN2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q96KN2.
    Bgeei Q96KN2.
    CleanExi HS_CNDP1.
    Genevestigatori Q96KN2.

    Family and domain databases

    Gene3Di 3.30.70.360. 1 hit.
    InterProi IPR001261. ArgE/DapE_CS.
    IPR017153. GSH_degradosome_DUG1.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view ]
    Pfami PF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037242. CNDP_dipeptidase. 1 hit.
    PROSITEi PS00759. ARGE_DAPE_CPG2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of a second human homologue of glutamate carboxypeptidase in peptidase family M20."
      Chen J.M., Barrett A.J.
      Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-113.
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS LEU-20 INS AND VAL-113.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-113.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS LEU-20 INS AND VAL-113.
      Tissue: Brain and Skin.
    6. "Human serum carnosinase: characterization, distinction from cellular carnosinase, and activation by cadmium."
      Lenney J.F., George R.P., Weiss A.M., Kucera C.M., Chan P.W., Rinzler G.S.
      Clin. Chim. Acta 123:221-231(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, SUBUNIT, CATALYTIC ACTIVITY.
    7. "Homocarnosinosis: lack of serum carnosinase is the defect probably responsible for elevated brain and CSF homocarnosine."
      Lenney J.F., Peppers S.C., Kucera C.M., Sjaastad O.
      Clin. Chim. Acta 132:157-165(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    8. "Sequence identification and characterization of human carnosinase and a closely related non-specific dipeptidase."
      Teufel M., Saudek V., Ledig J.P., Bernhardt A., Boularand S., Carreau A., Cairns N.J., Carter C., Cowley D.J., Duverger D., Ganzhorn A.J., Guenet C., Heintzelmann B., Laucher V., Sauvage C., Smirnova T.
      J. Biol. Chem. 278:6521-6531(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, CATALYTIC ACTIVITY, MUTAGENESIS OF HIS-132; ASP-165 AND GLU-200.
    9. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
      Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
      J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-322 AND ASN-382.
      Tissue: Plasma.
    10. "Crystal structure of human carnosine dipeptidase 1."
      Structural genomics consortium (SGC)
      Submitted (JUL-2011) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.26 ANGSTROMS) OF 27-507 IN COMPLEX WITH ZINC IONS, COFACTOR.

    Entry informationi

    Entry nameiCNDP1_HUMAN
    AccessioniPrimary (citable) accession number: Q96KN2
    Secondary accession number(s): Q14D40
    , Q17S05, Q2TBG0, Q6UWK2, Q9BT98
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2002
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 114 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 18
      Human chromosome 18: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. Peptidase families
      Classification of peptidase families and list of entries
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3