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Protein

N-terminal kinase-like protein

Gene

SCYL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum (PubMed:18556652). Involved in the maintenance of the Golgi apparatus morphology (PubMed:26581903). Has no detectable kinase activity in vitro (PubMed:18556652).2 Publications
Isoform 6 acts as transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region.1 Publication

GO - Molecular functioni

  • ATP binding Source: InterPro
  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • DNA binding Source: UniProtKB-KW
  • protein tyrosine kinase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

ER-Golgi transport, Transcription, Transcription regulation, Transport

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiQ96KG9.

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal kinase-like protein
Alternative name(s):
Coated vesicle-associated kinase of 90 kDa
SCY1-like protein 1
Telomerase regulation-associated protein
Telomerase transcriptional element-interacting factor
Teratoma-associated tyrosine kinase
Gene namesi
Name:SCYL1
Synonyms:CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP
ORF Names:HT019
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:14372. SCYL1.

Subcellular locationi

Isoform 1 :
  • Cytoplasm

  • Note: Cytoplasmic throughout the cell cycle.
Isoform 2 :
  • Cytoplasm

  • Note: Cytoplasmic throughout the cell cycle.
Isoform 3 :
Isoform 6 :

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • cis-Golgi network Source: UniProtKB
  • COPI vesicle coat Source: UniProtKB
  • cytoplasm Source: HGNC
  • endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • membrane Source: UniProtKB
  • microtubule organizing center Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Nucleus

Pathology & Biotechi

Involvement in diseasei

Spinocerebellar ataxia, autosomal recessive, 21 (SCAR21)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of spinocerebellar ataxia, a clinically and genetically heterogeneous group of cerebellar disorders due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR21 is characterized by cerebellar atrophy and ataxia with onset in early childhood. Patients also manifest recurrent episodes of liver failure, hepatic fibrosis and a peripheral neuropathy.
See also OMIM:616719

Keywords - Diseasei

Mental retardation, Neurodegeneration, Spinocerebellar ataxia

Organism-specific databases

DisGeNETi57410.
MIMi616719. phenotype.
OpenTargetsiENSG00000142186.
PharmGKBiPA31812.

Polymorphism and mutation databases

BioMutaiSCYL1.
DMDMi74762671.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002495411 – 808N-terminal kinase-like proteinAdd BLAST808

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei754PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ96KG9.
MaxQBiQ96KG9.
PaxDbiQ96KG9.
PeptideAtlasiQ96KG9.
PRIDEiQ96KG9.

PTM databases

iPTMnetiQ96KG9.
PhosphoSitePlusiQ96KG9.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000142186.
CleanExiHS_SCYL1.
ExpressionAtlasiQ96KG9. baseline and differential.
GenevisibleiQ96KG9. HS.

Organism-specific databases

HPAiHPA015015.

Interactioni

Subunit structurei

Interacts with GORAB. Interacts with COPA, COPB1 and COPB2 (By similarity). Homooligomer. Interacts with AP2B1.By similarity2 Publications

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL

Protein-protein interaction databases

BioGridi121512. 27 interactors.
IntActiQ96KG9. 20 interactors.
MINTiMINT-3055065.
STRINGi9606.ENSP00000270176.

Structurei

3D structure databases

ProteinModelPortaliQ96KG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 314Protein kinasePROSITE-ProRule annotationAdd BLAST301
Repeati350 – 388HEAT 1Add BLAST39
Repeati389 – 427HEAT 2Add BLAST39
Repeati507 – 545HEAT 3Add BLAST39

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni793 – 808Interaction with COPB1By similarityAdd BLAST16

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili761 – 797Sequence analysisAdd BLAST37

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi589 – 619Pro-richAdd BLAST31

Domaini

The protein kinase domain is predicted to be catalytically inactive.

Sequence similaritiesi

Belongs to the protein kinase superfamily.Curated
Contains 3 HEAT repeats.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1243. Eukaryota.
ENOG410XQTG. LUCA.
GeneTreeiENSGT00860000133812.
HOVERGENiHBG082065.
InParanoidiQ96KG9.
KOiK08876.
OMAiNEVRDGW.
OrthoDBiEOG091G02FF.
PhylomeDBiQ96KG9.
TreeFamiTF313435.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96KG9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWFFARDPVR DFPFELIPEP PEGGLPGPWA LHRGRKKATG SPVSIFVYDV
60 70 80 90 100
KPGAEEQTQV AKAAFKRFKT LRHPNILAYI DGLETEKCLH VVTEAVTPLG
110 120 130 140 150
IYLKARVEAG GLKELEISWG LHQIVKALSF LVNDCSLIHN NVCMAAVFVD
160 170 180 190 200
RAGEWKLGGL DYMYSAQGNG GGPPRKGIPE LEQYDPPELA DSSGRVVREK
210 220 230 240 250
WSADMWRLGC LIWEVFNGPL PRAAALRNPG KIPKTLVPHY CELVGANPKV
260 270 280 290 300
RPNPARFLQN CRAPGGFMSN RFVETNLFLE EIQIKEPAEK QKFFQELSKS
310 320 330 340 350
LDAFPEDFCR HKVLPQLLTA FEFGNAGAVV LTPLFKVGKF LSAEEYQQKI
360 370 380 390 400
IPVVVKMFSS TDRAMRIRLL QQMEQFIQYL DEPTVNTQIF PHVVHGFLDT
410 420 430 440 450
NPAIREQTVK SMLLLAPKLN EANLNVELMK HFARLQAKDE QGPIRCNTTV
460 470 480 490 500
CLGKIGSYLS ASTRHRVLTS AFSRATRDPF APSRVAGVLG FAATHNLYSM
510 520 530 540 550
NDCAQKILPV LCGLTVDPEK SVRDQAFKAI RSFLSKLESV SEDPTQLEEV
560 570 580 590 600
EKDVHAASSP GMGGAAASWA GWAVTGVSSL TSKLIRSHPT TAPTETNIPQ
610 620 630 640 650
RPTPEGVPAP APTPVPATPT TSGHWETQEE DKDTAEDSST ADRWDDEDWG
660 670 680 690 700
SLEQEAESVL AQQDDWSTGG QVSRASQVSN SDHKSSKSPE SDWSSWEAEG
710 720 730 740 750
SWEQGWQEPS SQEPPPDGTR LASEYNWGGP ESSDKGDPFA TLSARPSTQP
760 770 780 790 800
RPDSWGEDNW EGLETDSRQV KAELARKKRE ERRREMEAKR AERKVAKGPM

KLGARKLD
Length:808
Mass (Da):89,631
Last modified:December 1, 2001 - v1
Checksum:iE87A2957DDCCE937
GO
Isoform 2 (identifier: Q96KG9-2) [UniParc]FASTAAdd to basket
Also known as: Variant 1

The sequence of this isoform differs from the canonical sequence as follows:
     606-622: Missing.

Show »
Length:791
Mass (Da):88,089
Checksum:i79A8FA462666CBC5
GO
Isoform 3 (identifier: Q96KG9-3) [UniParc]FASTAAdd to basket
Also known as: Variant 2

The sequence of this isoform differs from the canonical sequence as follows:
     606-622: Missing.
     628-711: Missing.

Note: Non-canonical splice junctions.
Show »
Length:707
Mass (Da):78,696
Checksum:i2F2C81CD4EAF8225
GO
Isoform 4 (identifier: Q96KG9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     750-808: PRPDSWGEDN...PMKLGARKLD → DRSRLSWPGRSARSGGGRWRPNAPRGRWPRAP

Show »
Length:781
Mass (Da):86,312
Checksum:i7DC90CF2578622FE
GO
Isoform 5 (identifier: Q96KG9-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     603-626: TPEGVPAPAPTPVPATPTTSGHWE → SRPARRPLGDAGGGQGHSRGQQHC
     627-808: Missing.

Show »
Length:626
Mass (Da):69,238
Checksum:i43E36C7298D8BB33
GO
Isoform 6 (identifier: Q96KG9-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     678-808: VSNSDHKSSK...PMKLGARKLD → SPTGAAGKLR...APRGRWPRAP

Show »
Length:787
Mass (Da):86,371
Checksum:iBD673D007B1A79B9
GO

Sequence cautioni

The sequence AAG09726 differs from that shown. Reason: Frameshift at positions 289 and 307.Curated
The sequence AAG17902 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti168 – 169GN → AT in AAG17902 (PubMed:15504359).Curated2
Sequence conflicti530I → F in AAG09726 (Ref. 7) Curated1
Sequence conflicti574V → W in AAG09726 (Ref. 7) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_041364479P → L.1 PublicationCorresponds to variant rs55977709dbSNPEnsembl.1
Natural variantiVAR_041365495H → Y in a metastatic melanoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_041366663Q → H.1 PublicationCorresponds to variant rs56076708dbSNPEnsembl.1
Natural variantiVAR_041367755W → S.1 PublicationCorresponds to variant rs56077405dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020503603 – 626TPEGV…SGHWE → SRPARRPLGDAGGGQGHSRG QQHC in isoform 5. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_020504606 – 622Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_020505627 – 808Missing in isoform 5. 1 PublicationAdd BLAST182
Alternative sequenceiVSP_020506628 – 711Missing in isoform 3. 1 PublicationAdd BLAST84
Alternative sequenceiVSP_020507678 – 808VSNSD…ARKLD → SPTGAAGKLRAPGNRAGRSQ APRSHLLTVHGWPASITGVA QSPATRATPSLPCLHVPAPS RGQTLGVRTTGRASRLTVDR SRLSWPGRSARSGGGRWRPN APRGRWPRAP in isoform 6. 1 PublicationAdd BLAST131
Alternative sequenceiVSP_020508750 – 808PRPDS…ARKLD → DRSRLSWPGRSARSGGGRWR PNAPRGRWPRAP in isoform 4. 1 PublicationAdd BLAST59

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047077 mRNA. Translation: BAB55454.1.
AB051427 mRNA. Translation: BAB55458.1.
AB051428 mRNA. Translation: BAB55459.1.
AF297709 mRNA. Translation: AAG17902.1. Frameshift.
AP000769 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW74399.1.
BC009967 mRNA. Translation: AAH09967.2.
BC069233 mRNA. Translation: AAH69233.1.
AF255613 Genomic DNA. Translation: AAF81422.1.
AF225424 mRNA. Translation: AAG09726.1. Frameshift.
CCDSiCCDS41672.1. [Q96KG9-1]
CCDS44646.1. [Q96KG9-2]
RefSeqiNP_001041683.1. NM_001048218.1. [Q96KG9-2]
NP_065731.3. NM_020680.3. [Q96KG9-1]
XP_005274177.1. XM_005274120.3. [Q96KG9-4]
UniGeneiHs.238839.

Genome annotation databases

EnsembliENST00000270176; ENSP00000270176; ENSG00000142186. [Q96KG9-1]
ENST00000420247; ENSP00000408192; ENSG00000142186. [Q96KG9-2]
ENST00000533862; ENSP00000437254; ENSG00000142186. [Q96KG9-6]
GeneIDi57410.
KEGGihsa:57410.
UCSCiuc001oea.2. human. [Q96KG9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047077 mRNA. Translation: BAB55454.1.
AB051427 mRNA. Translation: BAB55458.1.
AB051428 mRNA. Translation: BAB55459.1.
AF297709 mRNA. Translation: AAG17902.1. Frameshift.
AP000769 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW74399.1.
BC009967 mRNA. Translation: AAH09967.2.
BC069233 mRNA. Translation: AAH69233.1.
AF255613 Genomic DNA. Translation: AAF81422.1.
AF225424 mRNA. Translation: AAG09726.1. Frameshift.
CCDSiCCDS41672.1. [Q96KG9-1]
CCDS44646.1. [Q96KG9-2]
RefSeqiNP_001041683.1. NM_001048218.1. [Q96KG9-2]
NP_065731.3. NM_020680.3. [Q96KG9-1]
XP_005274177.1. XM_005274120.3. [Q96KG9-4]
UniGeneiHs.238839.

3D structure databases

ProteinModelPortaliQ96KG9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121512. 27 interactors.
IntActiQ96KG9. 20 interactors.
MINTiMINT-3055065.
STRINGi9606.ENSP00000270176.

PTM databases

iPTMnetiQ96KG9.
PhosphoSitePlusiQ96KG9.

Polymorphism and mutation databases

BioMutaiSCYL1.
DMDMi74762671.

Proteomic databases

EPDiQ96KG9.
MaxQBiQ96KG9.
PaxDbiQ96KG9.
PeptideAtlasiQ96KG9.
PRIDEiQ96KG9.

Protocols and materials databases

DNASUi57410.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000270176; ENSP00000270176; ENSG00000142186. [Q96KG9-1]
ENST00000420247; ENSP00000408192; ENSG00000142186. [Q96KG9-2]
ENST00000533862; ENSP00000437254; ENSG00000142186. [Q96KG9-6]
GeneIDi57410.
KEGGihsa:57410.
UCSCiuc001oea.2. human. [Q96KG9-1]

Organism-specific databases

CTDi57410.
DisGeNETi57410.
GeneCardsiSCYL1.
HGNCiHGNC:14372. SCYL1.
HPAiHPA015015.
MIMi607982. gene.
616719. phenotype.
neXtProtiNX_Q96KG9.
OpenTargetsiENSG00000142186.
PharmGKBiPA31812.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1243. Eukaryota.
ENOG410XQTG. LUCA.
GeneTreeiENSGT00860000133812.
HOVERGENiHBG082065.
InParanoidiQ96KG9.
KOiK08876.
OMAiNEVRDGW.
OrthoDBiEOG091G02FF.
PhylomeDBiQ96KG9.
TreeFamiTF313435.

Enzyme and pathway databases

SignaLinkiQ96KG9.

Miscellaneous databases

ChiTaRSiSCYL1. human.
GeneWikiiSCYL1.
GenomeRNAii57410.
PROiQ96KG9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000142186.
CleanExiHS_SCYL1.
ExpressionAtlasiQ96KG9. baseline and differential.
GenevisibleiQ96KG9. HS.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSCYL1_HUMAN
AccessioniPrimary (citable) accession number: Q96KG9
Secondary accession number(s): A6NJF1
, Q96G50, Q96KG8, Q96KH1, Q9HAW5, Q9HBL3, Q9NR53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.