Q96KC8 (DNJC1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DnaJ homolog subfamily C member 1

Alternative name(s):
DnaJ protein homolog MTJ1

Gene names

Name:	DNAJC1
Synonyms:	HTJ1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May modulate protein synthesis By similarity.
Subunit structure	Interacts (via J domain) with HSPA5. Interacts (via cytosolic domain) with ribosomes By similarity. Interacts (via SANT domain) with SERPINA3. Ref.1
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity. Nucleus membrane; Single-pass type I membrane protein. Microsome membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Contains 1 J domain. Contains 2 SANT domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome Nucleus
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	DNA-binding
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of proteolysis Traceable author statement Ref.1. Source: UniProtKB regulation of protein secretion Inferred from direct assay Ref.1. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATPase activator activity Traceable author statement Ref.1. Source: UniProtKB DNA binding Inferred from electronic annotation. Source: UniProtKB-KW heat shock protein binding Inferred from electronic annotation. Source: InterPro unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SERPINA3	P01011	3	EBI-296550,EBI-296557

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 47

47

Potential

Chain

48 – 554

507

DnaJ homolog subfamily C member 1

PRO_0000071042

Regions

Topological domain

48 – 153

106

Lumenal By similarity

Transmembrane

154 – 174

21

Helical; Potential

Topological domain

175 – 554

380

Cytoplasmic By similarity

Domain

65 – 129

65

J

Domain

325 – 379

55

SANT 1

Domain

492 – 547

56

SANT 2

Amino acid modifications

Modified residue

363

1

Phosphoserine By similarity

Modified residue

365

1

Phosphothreonine By similarity

Modified residue

381

1

Phosphoserine Ref.6

Modified residue

430

1

Phosphoserine Ref.6 Ref.8

Modified residue

479

1

Phosphoserine By similarity

Modified residue

480

1

Phosphoserine Ref.7

Modified residue

484

1

Phosphoserine Ref.7

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

554

Helix Strand Turn

Details...

Helix

332 – 344

13

Helix

352 – 360

9

Helix

364 – 376

13

Turn

489 – 491

3

Helix

499 – 511

13

Beta strand

514 – 516

3

Helix

517 – 523

7

Helix

524 – 526

3

Beta strand

528 – 530

3

Sequences

	Sequence		Length	Mass (Da)
	Q96KC8 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 65EAAB16C65E2B46	FASTA	554	63,883
	10 20 30 40 50 60 MTAPCSQPAQ LPGRRQLGLV PFPPPPPRTP LLWLLLLLLA AVAPARGWES GDLELFDLVE 70 80 90 100 110 120 EVQLNFYQFL GVQQDASSAD IRKAYRKLSL TLHPDKNKDE NAETQFRQLV AIYEVLKDDE 130 140 150 160 170 180 RRQRYDDILI NGLPDWRQPV FYYRRVRKMS NAELALLLFI ILTVGHYAVV WSIYLEKQLD 190 200 210 220 230 240 ELLSRKKREK KKKTGSKSVD VSKLGASEKN ERLLMKPQWH DLLPCKLGIW FCLTLKALPH 250 260 270 280 290 300 LIQDAGQFYA KYKETRLKEK EDALTRTELE TLQKQKKVKK PKPEFPVYTP LETTYIQSYD 310 320 330 340 350 360 HGTSIEEIEE QMDDWLENRN RTQKKQAPEW TEEDLSQLTR SMVKFPGGTP GRWEKIAHEL 370 380 390 400 410 420 GRSVTDVTTK AKQLKDSVTC SPGMVRLSEL KSTVQNSRPI KTATTLPDDM ITQREDAEGV 430 440 450 460 470 480 AAEEEQEGDS GEQETGATDA RPRRRKPARL LEATAKPEPE EKSRAKRQKD FDIAEQNESS 490 500 510 520 530 540 DEESLRKERA RSAEEPWTQN QQKLLELALQ QYPRGSSDRW DKIARCVPSK SKEDCIARYK 550 LLVELVQKKK QAKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The SANT2 domain of the murine tumor cell DnaJ-like protein 1 human homologue interacts with alpha1-antichymotrypsin and kinetically interferes with its serpin inhibitory activity." Kroczynska B., Evangelista C.M., Samant S.S., Elguindi E.C., Blond S.Y. J. Biol. Chem. 279:11432-11443(2004) [PubMed: 14668352] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SERPINA3. Tissue: Liver.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
[3]	NHLBI resequencing and genotyping service (RS&G) Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-381 AND SER-430, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-480 AND SER-484, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[8]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-430, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"Solution structure of RSGI RUH-037, a Myb DNA-binding domain in human." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 327-385 AND 484-543.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY225122 mRNA. Translation: AAP50497.1.
AK026062 mRNA. Translation: BAB15343.1.
AK027263 mRNA. Translation: BAB55004.1.
EF444973 Genomic DNA. Translation: ACA05978.1.
EF444973 Genomic DNA. Translation: ACA05979.1.
AL445431, AL359697 Genomic DNA. Translation: CAI13668.1.
AL359697, AL445431 Genomic DNA. Translation: CAH73412.1.
BC110894 mRNA. Translation: AAI10895.1.

IPI

IPI00074870.

RefSeq

NP_071760.2. NM_022365.3.

UniGene

Hs.499000.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CQQ	NMR	-	A	327-385	[»]
2CQR	NMR	-	A	484-543	[»]

ProteinModelPortal

Q96KC8.

SMR

Q96KC8. Positions 64-134, 327-385, 485-543.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96KC8. 3 interactions.

MINT

MINT-1036349.

STRING

Q96KC8.

PTM databases

PhosphoSite

Q96KC8.

Polymorphism databases

DMDM

27805464.

Proteomic databases

PRIDE

Q96KC8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000376980; ENSP00000366179; ENSG00000136770.

GeneID

64215.

KEGG

hsa:64215.

UCSC

uc001irc.1. human.

Organism-specific databases

CTD

64215.

GeneCards

GC10M022085.

H-InvDB

HIX0008698.

HGNC

HGNC:20090. DNAJC1.

HPA

HPA013432.

MIM

611207. gene.

neXtProt

NX_Q96KC8.

PharmGKB

PA128394706.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG15033.

HOGENOM

HBG445892.

HOVERGEN

HBG051374.

InParanoid

Q96KC8.

OMA

YYRRMRK.

OrthoDB

EOG4GXFMP.

PhylomeDB

Q96KC8.

Gene expression databases

ArrayExpress

Q96KC8.

Bgee

Q96KC8.

CleanEx

HS_DNAJC1.

Genevestigator

Q96KC8.

GermOnline

ENSG00000136770. Homo sapiens.

Family and domain databases

InterPro

IPR001623. DnaJ_N.
IPR018253. Heat_shock_DnaJ_CS.
IPR009057. Homeodomain-like.
IPR012287. Homeodomain-rel.
IPR003095. Hsp_DnaJ.
IPR014778. Myb_DNA-bd.
IPR001005. SANT_DNA-bd.
IPR017884. SANT_eukarya.
[Graphical view]

Gene3D

G3DSA:1.10.287.110. DnaJ_N. 1 hit.
G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.

KO

K09521.

Pfam

PF00226. DnaJ. 1 hit.
PF00249. Myb_DNA-binding. 1 hit.
[Graphical view]

PRINTS

PR00625. JDOMAIN.

SMART

SM00271. DnaJ. 1 hit.
SM00717. SANT. 2 hits.
[Graphical view]

SUPFAM

SSF46565. DnaJ_N. 1 hit.
SSF46689. Homeodomain_like. 2 hits.

PROSITE

PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51293. SANT. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

66123.

SOURCE

Search...

Entry information

Entry name

DNJC1_HUMAN

Accession

Primary (citable) accession number: Q96KC8
Secondary accession number(s): B0YIZ8, Q5VX89, Q9H6B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.