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Protein

Lymphokine-activated killer T-cell-originated protein kinase

Gene

PBK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64ATPPROSITE-ProRule annotation1
Active sitei167Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 46ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS09691-MONOMER.
SignaLinkiQ96KB5.
SIGNORiQ96KB5.

Names & Taxonomyi

Protein namesi
Recommended name:
Lymphokine-activated killer T-cell-originated protein kinase (EC:2.7.12.2)
Alternative name(s):
Cancer/testis antigen 84
Short name:
CT84
MAPKK-like protein kinase
Nori-3
PDZ-binding kinase
Spermatogenesis-related protein kinase
Short name:
SPK
T-LAK cell-originated protein kinase
Gene namesi
Name:PBK
Synonyms:TOPK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:18282. PBK.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: CACAO
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi9T → E: TP53-binding. 1 Publication1
Mutagenesisi64 – 65KK → AA: Loss of activity. 1 Publication2
Mutagenesisi320T → A: Decrease in the binding to DLG1. 1 Publication1
Mutagenesisi322V → A: Decrease in the binding to DLG1. 1 Publication1

Organism-specific databases

DisGeNETi55872.
OpenTargetsiENSG00000168078.
PharmGKBiPA134925802.

Chemistry databases

ChEMBLiCHEMBL4896.
GuidetoPHARMACOLOGYi2140.

Polymorphism and mutation databases

BioMutaiPBK.
DMDMi83305809.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867631 – 322Lymphokine-activated killer T-cell-originated protein kinaseAdd BLAST322

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei9PhosphothreonineCombined sources1
Modified residuei24PhosphothreonineCombined sources1
Modified residuei32PhosphoserineCombined sources1
Modified residuei59PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated; in a cell-cycle dependent manner at mitosis.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ96KB5.
PaxDbiQ96KB5.
PeptideAtlasiQ96KB5.
PRIDEiQ96KB5.

PTM databases

iPTMnetiQ96KB5.
PhosphoSitePlusiQ96KB5.

Expressioni

Tissue specificityi

Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.2 Publications

Gene expression databases

BgeeiENSG00000168078.
CleanExiHS_PBK.
ExpressionAtlasiQ96KB5. baseline and differential.
GenevisibleiQ96KB5. HS.

Organism-specific databases

HPAiHPA005753.

Interactioni

Subunit structurei

Interacts with DLG1 and TP53.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARAFP103983EBI-536853,EBI-365961
TP53P046377EBI-536853,EBI-366083

Protein-protein interaction databases

BioGridi120971. 29 interactors.
IntActiQ96KB5. 11 interactors.
MINTiMINT-130347.
STRINGi9606.ENSP00000301905.

Structurei

Secondary structure

1322
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 29Combined sources4
Helixi34 – 37Combined sources4
Beta strandi44 – 48Combined sources5
Helixi54 – 57Combined sources4
Helixi60 – 65Combined sources6
Helixi69 – 71Combined sources3
Helixi73 – 77Combined sources5
Beta strandi78 – 82Combined sources5
Helixi83 – 91Combined sources9
Beta strandi100 – 105Combined sources6
Beta strandi108 – 115Combined sources8
Beta strandi120 – 122Combined sources3
Helixi123 – 131Combined sources9
Turni132 – 134Combined sources3
Helixi140 – 159Combined sources20
Helixi170 – 172Combined sources3
Beta strandi173 – 176Combined sources4
Turni177 – 180Combined sources4
Beta strandi181 – 184Combined sources4
Beta strandi189 – 192Combined sources4
Helixi210 – 212Combined sources3
Helixi215 – 217Combined sources3
Beta strandi219 – 221Combined sources3
Helixi227 – 242Combined sources16
Beta strandi250 – 252Combined sources3
Turni254 – 256Combined sources3
Helixi263 – 265Combined sources3
Helixi268 – 274Combined sources7
Turni283 – 285Combined sources3
Helixi291 – 300Combined sources10
Helixi305 – 307Combined sources3
Helixi311 – 319Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5J0AX-ray2.74A23-320[»]
B19-319[»]
ProteinModelPortaliQ96KB5.
SMRiQ96KB5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 322Protein kinasePROSITE-ProRule annotationAdd BLAST291

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni320 – 322PDZ-interaction3

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0192. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00720000108839.
HOGENOMiHOG000294208.
HOVERGENiHBG056011.
InParanoidiQ96KB5.
KOiK08865.
OMAiHMARGLK.
OrthoDBiEOG091G05V7.
PhylomeDBiQ96KB5.
TreeFamiTF329763.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96KB5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGISNFKTP SKLSEKKKSV LCSTPTINIP ASPFMQKLGF GTGVNVYLMK
60 70 80 90 100
RSPRGLSHSP WAVKKINPIC NDHYRSVYQK RLMDEAKILK SLHHPNIVGY
110 120 130 140 150
RAFTEANDGS LCLAMEYGGE KSLNDLIEER YKASQDPFPA AIILKVALNM
160 170 180 190 200
ARGLKYLHQE KKLLHGDIKS SNVVIKGDFE TIKICDVGVS LPLDENMTVT
210 220 230 240 250
DPEACYIGTE PWKPKEAVEE NGVITDKADI FAFGLTLWEM MTLSIPHINL
260 270 280 290 300
SNDDDDEDKT FDESDFDDEA YYAALGTRPP INMEELDESY QKVIELFSVC
310 320
TNEDPKDRPS AAHIVEALET DV
Length:322
Mass (Da):36,085
Last modified:December 6, 2005 - v3
Checksum:i6BF55789BC204ABE
GO
Isoform 2 (identifier: Q96KB5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     198-198: T → TAPAFITILLVS

Note: No experimental confirmation available.
Show »
Length:333
Mass (Da):37,211
Checksum:i05A472E8DD4D9928
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34F → I in AAF71521 (PubMed:11378444).Curated1
Sequence conflicti169K → E in BAB55019 (PubMed:14702039).Curated1
Sequence conflicti254D → N in BAB55019 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_021162107N → S.4 PublicationsCorresponds to variant rs3779620dbSNPEnsembl.1
Natural variantiVAR_051676220E → D.Corresponds to variant rs17057901dbSNPEnsembl.1
Natural variantiVAR_041234241M → L.1 PublicationCorresponds to variant rs36086402dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055269198T → TAPAFITILLVS in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027249 mRNA. Translation: BAA99576.1.
AB027250 mRNA. Translation: BAA99577.1.
AF189722 mRNA. Translation: AAF69107.1.
AF237709 mRNA. Translation: AAF71521.1.
AK027291 mRNA. Translation: BAB55019.1.
AK301836 mRNA. Translation: BAG63280.1.
AC104997 Genomic DNA. No translation available.
CH471080 Genomic DNA. Translation: EAW63536.1.
CH471080 Genomic DNA. Translation: EAW63537.1.
BC015191 mRNA. Translation: AAH15191.1.
CCDSiCCDS6063.1. [Q96KB5-1]
CCDS64858.1. [Q96KB5-2]
RefSeqiNP_001265874.1. NM_001278945.1. [Q96KB5-2]
NP_060962.2. NM_018492.3. [Q96KB5-1]
XP_006716431.1. XM_006716368.2. [Q96KB5-1]
UniGeneiHs.104741.

Genome annotation databases

EnsembliENST00000301905; ENSP00000301905; ENSG00000168078. [Q96KB5-1]
ENST00000522944; ENSP00000428489; ENSG00000168078. [Q96KB5-2]
GeneIDi55872.
KEGGihsa:55872.
UCSCiuc011lap.4. human. [Q96KB5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027249 mRNA. Translation: BAA99576.1.
AB027250 mRNA. Translation: BAA99577.1.
AF189722 mRNA. Translation: AAF69107.1.
AF237709 mRNA. Translation: AAF71521.1.
AK027291 mRNA. Translation: BAB55019.1.
AK301836 mRNA. Translation: BAG63280.1.
AC104997 Genomic DNA. No translation available.
CH471080 Genomic DNA. Translation: EAW63536.1.
CH471080 Genomic DNA. Translation: EAW63537.1.
BC015191 mRNA. Translation: AAH15191.1.
CCDSiCCDS6063.1. [Q96KB5-1]
CCDS64858.1. [Q96KB5-2]
RefSeqiNP_001265874.1. NM_001278945.1. [Q96KB5-2]
NP_060962.2. NM_018492.3. [Q96KB5-1]
XP_006716431.1. XM_006716368.2. [Q96KB5-1]
UniGeneiHs.104741.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5J0AX-ray2.74A23-320[»]
B19-319[»]
ProteinModelPortaliQ96KB5.
SMRiQ96KB5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120971. 29 interactors.
IntActiQ96KB5. 11 interactors.
MINTiMINT-130347.
STRINGi9606.ENSP00000301905.

Chemistry databases

ChEMBLiCHEMBL4896.
GuidetoPHARMACOLOGYi2140.

PTM databases

iPTMnetiQ96KB5.
PhosphoSitePlusiQ96KB5.

Polymorphism and mutation databases

BioMutaiPBK.
DMDMi83305809.

Proteomic databases

EPDiQ96KB5.
PaxDbiQ96KB5.
PeptideAtlasiQ96KB5.
PRIDEiQ96KB5.

Protocols and materials databases

DNASUi55872.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000301905; ENSP00000301905; ENSG00000168078. [Q96KB5-1]
ENST00000522944; ENSP00000428489; ENSG00000168078. [Q96KB5-2]
GeneIDi55872.
KEGGihsa:55872.
UCSCiuc011lap.4. human. [Q96KB5-1]

Organism-specific databases

CTDi55872.
DisGeNETi55872.
GeneCardsiPBK.
HGNCiHGNC:18282. PBK.
HPAiHPA005753.
MIMi611210. gene.
neXtProtiNX_Q96KB5.
OpenTargetsiENSG00000168078.
PharmGKBiPA134925802.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0192. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00720000108839.
HOGENOMiHOG000294208.
HOVERGENiHBG056011.
InParanoidiQ96KB5.
KOiK08865.
OMAiHMARGLK.
OrthoDBiEOG091G05V7.
PhylomeDBiQ96KB5.
TreeFamiTF329763.

Enzyme and pathway databases

BioCyciZFISH:HS09691-MONOMER.
SignaLinkiQ96KB5.
SIGNORiQ96KB5.

Miscellaneous databases

ChiTaRSiPBK. human.
GeneWikiiPBK_(gene).
GenomeRNAii55872.
PROiQ96KB5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168078.
CleanExiHS_PBK.
ExpressionAtlasiQ96KB5. baseline and differential.
GenevisibleiQ96KB5. HS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOPK_HUMAN
AccessioniPrimary (citable) accession number: Q96KB5
Secondary accession number(s): B4DX68
, D3DST2, Q9NPD9, Q9NYL7, Q9NZK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: December 6, 2005
Last modified: November 30, 2016
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.