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Protein

Abscission/NoCut checkpoint regulator

Gene

ZFYVE19

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key regulator of abscission step in cytokinesis: part of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Together with CHMP4C, required to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ZFYVE19/ANCHR and VPS4 and subsequent abscission.1 Publication

Caution

Phosphorylated in vitro at Ser-22 by AURKB; however, phosphorylation at this site could not be confirmed in vivo.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri74 – 133FYVE-typePROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • phosphatidylinositol-3-phosphate binding Source: UniProtKB

GO - Biological processi

  • abscission Source: UniProtKB
  • cell division Source: UniProtKB-KW
  • mitotic cytokinesis checkpoint Source: UniProtKB
  • negative regulation of cytokinesis Source: UniProtKB

Keywordsi

Biological processCell cycle, Cell division
LigandLipid-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Abscission/NoCut checkpoint regulator
Short name:
ANCHR
Alternative name(s):
MLL partner containing FYVE domain
Zinc finger FYVE domain-containing protein 19
Gene namesi
Name:ZFYVE19
Synonyms:ANCHR, MPFYVE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000166140.17
HGNCiHGNC:20758 ZFYVE19
neXtProtiNX_Q96K21

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving ZFYVE19 is associated with acute myeloblastic leukemia (AML). Translocation t(11;15)(q23;q14) with KMT2A/MLL1 (PubMed:12618766).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi101R → A: Abolishes binding to phosphatidylinositol-3-phosphate (PtdIns(3)P) without affecting localization to the midbody. 1 Publication1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei145 – 146Breakpoint for translocation to form KMT2A/MLL1-ZFYVE192

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNETi84936
OpenTargetsiENSG00000166140
PharmGKBiPA134906967

Polymorphism and mutation databases

BioMutaiZFYVE19
DMDMi296453076

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000987181 – 471Abscission/NoCut checkpoint regulatorAdd BLAST471

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei144PhosphoserineCombined sources1
Cross-linki207Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei243PhosphothreonineCombined sources1
Modified residuei293PhosphoserineCombined sources1
Modified residuei354PhosphoserineCombined sources1
Modified residuei463PhosphoserineCombined sources1
Isoform 3 (identifier: Q96K21-3)
Modified residuei286PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ96K21
MaxQBiQ96K21
PaxDbiQ96K21
PeptideAtlasiQ96K21
PRIDEiQ96K21

PTM databases

iPTMnetiQ96K21
PhosphoSitePlusiQ96K21

Expressioni

Tissue specificityi

Detected in brain, heart, skeletal muscle, kidney and liver.1 Publication

Gene expression databases

BgeeiENSG00000166140
CleanExiHS_ZFYVE19
ExpressionAtlasiQ96K21 baseline and differential
GenevisibleiQ96K21 HS

Organism-specific databases

HPAiHPA040283
HPA041946

Interactioni

Subunit structurei

Interacts (via MIM1-B) with VPS4A; interaction takes place at the midbody ring following cytokinesis checkpoint activation.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi12437043 interactors.
DIPiDIP-60854N
IntActiQ96K21 10 interactors.
STRINGi9606.ENSP00000347498

Structurei

3D structure databases

ProteinModelPortaliQ96K21
SMRiQ96K21
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili311 – 375Sequence analysisAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi174 – 187MIM1-AAdd BLAST14
Motifi326 – 339MIM1-BAdd BLAST14

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi412 – 417Poly-Glu6
Compositional biasi421 – 444Cys-richAdd BLAST24

Domaini

The FYVE-type zinc finger mediates binding to phosphatidylinositol-3-phosphate (PtdIns3P).1 Publication
The MIM1-B motif mediates interaction with VPS4A.1 Publication

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri74 – 133FYVE-typePROSITE-ProRule annotationAdd BLAST60

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1818 Eukaryota
ENOG410XNRF LUCA
GeneTreeiENSGT00390000016108
HOGENOMiHOG000231992
HOVERGENiHBG057281
InParanoidiQ96K21
OMAiRCGNTQQ
OrthoDBiEOG091G0F4F
PhylomeDBiQ96K21
TreeFamiTF317652

Family and domain databases

Gene3Di3.30.40.101 hit
InterProiView protein in InterPro
IPR000306 Znf_FYVE
IPR017455 Znf_FYVE-rel
IPR011011 Znf_FYVE_PHD
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF01363 FYVE, 1 hit
SMARTiView protein in SMART
SM00064 FYVE, 1 hit
SUPFAMiSSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS50178 ZF_FYVE, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96K21-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNYDSQQPPL PPLPYAGCRR ASGFPALGRG GTVPVGVWGG AGQGREGRSW
60 70 80 90 100
GEGPRGPGLG RRDLSSADPA VLGATMESRC YGCAVKFTLF KKEYGCKNCG
110 120 130 140 150
RAFCSGCLSF SAAVPRTGNT QQKVCKQCHE VLTRGSSANA SKWSPPQNYK
160 170 180 190 200
KRVAALEAKQ KPSTSQSQGL TRQDQMIAER LARLRQENKP KLVPSQAEIE
210 220 230 240 250
ARLAALKDER QGSIPSTQEM EARLAALQGR VLPSQTPQPA HHTPDTRTQA
260 270 280 290 300
QQTQDLLTQL AAEVAIDESW KGGGPAASLQ NDLNQGGPGS TNSKRQANWS
310 320 330 340 350
LEEEKSRLLA EAALELREEN TRQERILALA KRLAMLRGQD PERVTLQDYR
360 370 380 390 400
LPDSDDDEDE ETAIQRVLQQ LTEEASLDEA SGFNIPAEQA SRPWTQPRGA
410 420 430 440 450
EPEAQDVDPR PEAEEEELPW CCICNEDATL RCAGCDGDLF CARCFREGHD
460 470
AFELKEHQTS AYSPPRAGQE H
Length:471
Mass (Da):51,546
Last modified:May 18, 2010 - v3
Checksum:i0F07D2DDFD19864D
GO
Isoform 2 (identifier: Q96K21-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-93: MNYDSQQPPL...AVKFTLFKKE → MPAVAEALGQ...DLPDSSGKLQ

Show »
Length:461
Mass (Da):50,534
Checksum:i0366891B7E766686
GO
Isoform 3 (identifier: Q96K21-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     276-343: Missing.

Note: No experimental confirmation available.Combined sources
Show »
Length:403
Mass (Da):43,934
Checksum:iF2D8DF956362DABF
GO
Isoform 4 (identifier: Q96K21-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-175: Missing.

Note: No experimental confirmation available.
Show »
Length:296
Mass (Da):32,963
Checksum:i53D85D6E28C521A1
GO

Sequence cautioni

The sequence AAH21092 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAO73862 differs from that shown. Reason: Frameshift at position 459.Curated
The sequence BAB55338 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104C → R in AAH21092 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_057494210R → H. Corresponds to variant dbSNP:rs34819163Ensembl.1
Natural variantiVAR_060474376S → A2 PublicationsCorresponds to variant dbSNP:rs690347Ensembl.1
Natural variantiVAR_060475398R → C. Corresponds to variant dbSNP:rs72735636Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0460081 – 175Missing in isoform 4. 1 PublicationAdd BLAST175
Alternative sequenceiVSP_0137911 – 93MNYDS…LFKKE → MPAVAEALGQEGPPDLSRSA FLATVLTSLSAAFSSMPSSA YSLPFSRSLELDYHTSSCFR GTMVKADCPVPITDLPDSSG KLQ in isoform 2. 1 PublicationAdd BLAST93
Alternative sequenceiVSP_013792276 – 343Missing in isoform 3. 1 PublicationAdd BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF445414 mRNA Translation: AAO73862.1 Frameshift.
AK027746 mRNA Translation: BAB55338.1 Different initiation.
AK122779 mRNA Translation: BAG53724.1
AC012476 Genomic DNA No translation available.
CH471125 Genomic DNA Translation: EAW92451.1
BC021092 mRNA Translation: AAH21092.1 Different initiation.
CCDSiCCDS42025.1 [Q96K21-1]
CCDS58353.1 [Q96K21-2]
CCDS58354.1 [Q96K21-3]
CCDS58355.1 [Q96K21-4]
RefSeqiNP_001070736.1, NM_001077268.1 [Q96K21-1]
NP_001245349.1, NM_001258420.1 [Q96K21-3]
NP_001245350.1, NM_001258421.1 [Q96K21-4]
NP_116239.3, NM_032850.4 [Q96K21-2]
UniGeneiHs.121676

Genome annotation databases

EnsembliENST00000299173; ENSP00000299173; ENSG00000166140 [Q96K21-3]
ENST00000336455; ENSP00000337824; ENSG00000166140 [Q96K21-2]
ENST00000355341; ENSP00000347498; ENSG00000166140 [Q96K21-1]
ENST00000564258; ENSP00000457617; ENSG00000166140 [Q96K21-4]
GeneIDi84936
KEGGihsa:84936
UCSCiuc001zmt.2 human [Q96K21-1]

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiANCHR_HUMAN
AccessioniPrimary (citable) accession number: Q96K21
Secondary accession number(s): B3KVB2
, C9JNF4, H3BUF9, Q86WC2, Q8WU96
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: May 18, 2010
Last modified: March 28, 2018
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome