PDZ and LIM domain protein 2 - Homo sapiens (Human)

Preferred order of sections

Names and origin

Protein names

Recommended name:
PDZ and LIM domain protein 2

Alternative name(s):
PDZ-LIM protein mystique

Gene names

Name:	PDLIM2
ORF Names:	PP6345

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity. Ref.9
Subunit structure	Interacts with alpha-actinins ACTN1 and ACTN4, FLNA and MYH9 By similarity.
Subcellular location	Cytoplasm. Nucleus Ref.9. Note: May be partially nuclear. Ref.9 Isoform 1: Cytoplasm › cytoskeleton Ref.9. Note: Colocalizes with beta-1 integrin (ITGB1) and alpha-actinin but not with paxillin (PXN). Ref.9 Isoform 2: Cytoplasm › cytoskeleton Ref.9. Isoform 3: Nucleus Ref.9.
Sequence similarities	Contains 1 LIM zinc-binding domain. Contains 1 PDZ (DHR) domain.
Sequence caution	The sequence AAG16633.1 differs from that shown. Reason: Frameshift at position 131. The sequence AAL55747.1 differs from that shown. Reason: Frameshift at positions 42 and 207. The sequence AAL65265.1 differs from that shown. Reason: Frameshift at position 237. The sequence BAB15788.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Nucleus
Coding sequence diversity	Alternative splicing
Domain	LIM domain
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	actin cytoskeleton Inferred from direct assay. Source: HPA cell surface Inferred from direct assay PubMed 12493773. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell focal adhesion Inferred from direct assay. Source: HPA nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96JY6-1) Also known as: Mystique 2; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96JY6-2) The sequence of this isoform differs from the canonical sequence as follows: 154-224: SFQSLACSPG...LLDEDSEVFK → RRPRPRWRLG...LSALAGSPGG 225-352: Missing.
Note: No experimental confirmation available.

Isoform 3 (identifier: Q96JY6-3) Also known as: Mystique 1; The sequence of this isoform differs from the canonical sequence as follows: 328-352: VGDELYCEKHARQRYSAPATLSSRA → EDACAMEGMRLSLEALEGMVEGAKRRDRRKTRRPIQPSW

Isoform 4 (identifier: Q96JY6-4) Also known as: Mystique 3; The sequence of this isoform differs from the canonical sequence as follows: 256-352: GTPAFLPSSL...SAPATLSSRA → TRLCASRRAGTATPAATPVPTVG

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 352

352

PDZ and LIM domain protein 2

PRO_0000075862

Regions

Domain

1 – 84

84

PDZ

Domain

284 – 344

61

LIM zinc-binding

Compositional bias

114 – 161

48

Ser-rich

Amino acid modifications

Modified residue

197

1

Phosphoserine By similarity

Natural variations

Alternative sequence

154 – 224

71

SFQSL…SEVFK → RRPRPRWRLGGAGAAAFPGP SFLQAQHGLGRGKPPPGRGL GSLQDAAGKSRGTGGPPTVQ LLSALAGSPGG in isoform 2.

VSP_014058

Alternative sequence

225 – 352

128

Missing in isoform 2.

VSP_014059

Alternative sequence

256 – 352

97

GTPAF…LSSRA → TRLCASRRAGTATPAATPVP TVG in isoform 4.

VSP_014060

Alternative sequence

328 – 352

25

VGDEL…LSSRA → EDACAMEGMRLSLEALEGMV EGAKRRDRRKTRRPIQPSW in isoform 3.

VSP_014061

Experimental info

Mutagenesis

80

1

L → K: Abolishes cell adhesion to collagen and ability to suppress anchorage independent growth. Ref.9

Mutagenesis

313

1

C → S: Abolishes ability to suppress anchorage independent growth but not cell adhesion to collagen; when associated with S-316. Ref.9

Mutagenesis

316

1

C → S: Abolishes ability to suppress anchorage independent growth but not cell adhesion to collagen; when associated with S-313. Ref.9

Sequence conflict

91

1

T → N in AAG16633. Ref.1

Sequence conflict

116

1

S → F in AAG16633. Ref.1

Sequence conflict

149

1

A → R Ref.3

Secondary structure

1

.

352

Helix Strand Turn

Details...

Sequences

Sequence		Length	Mass (Da)
Isoform 1 (Mystique 2) [UniParc]. Last modified December 1, 2001. Version 1. Checksum: E36EC66C61C5E9DB Show »	FASTA	352	37,459
10 20 30 40 50 60 MALTVDVAGP APWGFRITGG RDFHTPIMVT KVAERGKAKD ADLRPGDIIV AINGESAEGM 70 80 90 100 110 120 LHAEAQSKIR QSPSPLRLQL DRSQATSPGQ TNGDSSLEVL ATRFQGSVRT YTESQSSLRS 130 140 150 160 170 180 SYSSPTSLSP RAGSPFSPPP SSSSLTGEAA ISRSFQSLAC SPGLPAADRL SYSGRPGSRQ 190 200 210 220 230 240 AGLGRAGDSA VLVLPPSPGP RSSRPSMDSE GGSLLLDEDS EVFKMLQENR EGRAAPRQSS 250 260 270 280 290 300 SFRLLQEALE AEERGGTPAF LPSSLSPQSS LPASRALATP PKLHTCEKCS TSIANQAVRI 310 320 330 340 350 QEGRYRHPGC YTCADCGLNL KMRGHFWVGD ELYCEKHARQ RYSAPATLSS RA « Hide
Isoform 2 [UniParc]. Checksum: F4C39E4F01144F52 Show »	FASTA	224	23,073
10 20 30 40 50 60 MALTVDVAGP APWGFRITGG RDFHTPIMVT KVAERGKAKD ADLRPGDIIV AINGESAEGM 70 80 90 100 110 120 LHAEAQSKIR QSPSPLRLQL DRSQATSPGQ TNGDSSLEVL ATRFQGSVRT YTESQSSLRS 130 140 150 160 170 180 SYSSPTSLSP RAGSPFSPPP SSSSLTGEAA ISRRRPRPRW RLGGAGAAAF PGPSFLQAQH 190 200 210 220 GLGRGKPPPG RGLGSLQDAA GKSRGTGGPP TVQLLSALAG SPGG « Hide
Isoform 3 (Mystique 1) [UniParc]. Checksum: 739E0BB65889B1F6 Show »	FASTA	366	39,181
10 20 30 40 50 60 MALTVDVAGP APWGFRITGG RDFHTPIMVT KVAERGKAKD ADLRPGDIIV AINGESAEGM 70 80 90 100 110 120 LHAEAQSKIR QSPSPLRLQL DRSQATSPGQ TNGDSSLEVL ATRFQGSVRT YTESQSSLRS 130 140 150 160 170 180 SYSSPTSLSP RAGSPFSPPP SSSSLTGEAA ISRSFQSLAC SPGLPAADRL SYSGRPGSRQ 190 200 210 220 230 240 AGLGRAGDSA VLVLPPSPGP RSSRPSMDSE GGSLLLDEDS EVFKMLQENR EGRAAPRQSS 250 260 270 280 290 300 SFRLLQEALE AEERGGTPAF LPSSLSPQSS LPASRALATP PKLHTCEKCS TSIANQAVRI 310 320 330 340 350 360 QEGRYRHPGC YTCADCGLNL KMRGHFWEDA CAMEGMRLSL EALEGMVEGA KRRDRRKTRR PIQPSW « Hide
Isoform 4 (Mystique 3) [UniParc]. Checksum: F3D480E6DEAD66E9 Show »	FASTA	278	29,105
10 20 30 40 50 60 MALTVDVAGP APWGFRITGG RDFHTPIMVT KVAERGKAKD ADLRPGDIIV AINGESAEGM 70 80 90 100 110 120 LHAEAQSKIR QSPSPLRLQL DRSQATSPGQ TNGDSSLEVL ATRFQGSVRT YTESQSSLRS 130 140 150 160 170 180 SYSSPTSLSP RAGSPFSPPP SSSSLTGEAA ISRSFQSLAC SPGLPAADRL SYSGRPGSRQ 190 200 210 220 230 240 AGLGRAGDSA VLVLPPSPGP RSSRPSMDSE GGSLLLDEDS EVFKMLQENR EGRAAPRQSS 250 260 270 SFRLLQEALE AEERGTRLCA SRRAGTATPA ATPVPTVG « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mystique, a PDZ-LIM protein with one carboxyl terminal LIM domain." Lau Y.M., Kotaka M., Chan A.H., Lee S.M.Y., Au C.C., Chim S.S.C., Kok L.D.S., Ng E.K.O., Siu S.S., Yiu S.W.H., Fung K.P., Waye M.M.Y., Tsui S.K.W., Lee C.Y. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	Guo J.H., Zan Q., Yu L. Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Hypothalamus.
[3]	"Cloning a novel protein containing PDZ and LIM domains." Shan Y.X., Yu L., Huang C.Q. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[4]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Characterization of long cDNA clones from human adult spleen." Hattori A., Okumura K., Nagase T., Kikuno R., Hirosawa M., Ohara O. DNA Res. 7:357-366(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Spleen.
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta and Spleen.
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Placenta.
[9]	"Mystique is a new IGF-I regulated PDZ-LIM domain protein that promotes cell attachment and migration and suppresses anchorage independent growth." Loughran G., Healy N., Kiely P.A., Huigsloot M., Kedersha N.L., O'connor R. Mol. Biol. Cell 16:1811-1822(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-80; CYS-313 AND CYS-316.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[11]	"Structure of the PDZ domain of human PDLIM2 bound to a C-terminal extension from human beta-tropomyosin." Structural genomics consortium (SGC) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 1-82.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY007729 mRNA. Translation: AAG16633.1. Frameshift.
AY070438 mRNA. Translation: AAL65265.1. Frameshift.
AY217349 mRNA. Translation: AAO45102.1.
AF289563 mRNA. Translation: AAL55747.1. Frameshift.
AK024498 mRNA. Translation: BAB15788.1. Different initiation.
AK027800 mRNA. Translation: BAB55378.1.
CH471080 Genomic DNA. Translation: EAW63667.1.
CH471080 Genomic DNA. Translation: EAW63669.1.
BC021556 mRNA. Translation: AAH21556.1.
BC071774 mRNA. Translation: AAH71774.1.

IPI

IPI00007983.
IPI00383278.
IPI00386717.
IPI00396593.

RefSeq

NP_067643.3. NM_021630.5.
NP_789847.1. NM_176871.3.
NP_932159.1. NM_198042.3.

UniGene

Hs.632034.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PA1	X-ray	1.70	A	1-82	[»]
3PDV	X-ray	2.20	A	1-83	[»]

ProteinModelPortal

Q96JY6.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000265810.

PTM databases

PhosphoSite

Q96JY6.

Proteomic databases

PaxDb

Q96JY6.

PRIDE

Q96JY6.

Protocols and materials databases

DNASU

64236.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000265810; ENSP00000265810; ENSG00000120913.
ENST00000339162; ENSP00000342035; ENSG00000120913.
ENST00000397760; ENSP00000380867; ENSG00000120913.
ENST00000397761; ENSP00000380868; ENSG00000120913.
ENST00000409141; ENSP00000386868; ENSG00000120913.
ENST00000409417; ENSP00000387084; ENSG00000120913.

GeneID

64236.

KEGG

hsa:64236.

UCSC

uc003xby.3. human.
uc003xca.3. human.
uc003xcc.2. human.

Organism-specific databases

CTD

64236.

GeneCards

GC08P022435.

HGNC

HGNC:13992. PDLIM2.

HPA

HPA003880.

MIM

609722. gene.

neXtProt

NX_Q96JY6.

PharmGKB

PA33159.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG246155.

HOGENOM

HOG000290704.

HOVERGEN

HBG061371.

OMA

FQSLAHS.

Gene expression databases

ArrayExpress

Q96JY6.

Bgee

Q96JY6.

CleanEx

HS_PDLIM2.

Genevestigator

Q96JY6.

Family and domain databases

Gene3D

2.10.110.10. 1 hit.

InterPro

IPR001478. PDZ.
IPR001781. Znf_LIM.
[Graphical view]

Pfam

PF00412. LIM. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]

SMART

SM00132. LIM. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]

SUPFAM

SSF50156. PDZ. 1 hit.

PROSITE

PS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q96JY6.

GenomeRNAi

64236.

NextBio

66179.

SOURCE

Search...

Entry information

Entry name

PDLI2_HUMAN

Accession

Primary (citable) accession number: Q96JY6
Secondary accession number(s): D3DSR5

Q9H7I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families