Q96JP5 (ZFP91_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase ZFP91
EC=6.3.2.-

Alternative name(s):
Zinc finger protein 757
Zinc finger protein 91 homolog
Short name=Zfp-91

Gene names

Name:	ZFP91
Synonyms:	ZNF757
ORF Names:	FKSG11

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Atypical E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabilize and activate MAP3K14/NIK. It thereby acts as an activator of the non-canonical NF-kappa-B2/NFKB2 pathway. May also play an important role in cell proliferation and/or anti-apoptosis. Ref.1 Ref.9
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with MAP3K14/NIK. Ref.9
Subcellular location	Nucleus Ref.1.
Tissue specificity	Expressed ubiquitously, particularly at high level in testis. Isoform 2 is testis specific.
Miscellaneous	In contrast to other E3 ubiquitin-protein ligase, does not contain any domain (RING-type zinc finger or HECT domain) known to mediate E3 ligase activity.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers.
Sequence caution	The sequence AAL09963.1 differs from that shown. Reason: Frameshift at positions 72 and 80.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	activation of NF-kappaB-inducing kinase activity Inferred from direct assay Ref.9. Source: UniProtKB protein K63-linked ubiquitination Inferred from direct assay Ref.9. Source: UniProtKB
Cellular_component	nucleus Inferred from direct assay. Source: HPA
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro ubiquitin-protein ligase activity Inferred from direct assay Ref.9. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96JP5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96JP5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     526-529: VSLM → SIHR
     530-570: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 570	569	E3 ubiquitin-protein ligase ZFP91		PRO_0000047312
Regions
	Zinc finger	311 – 336	26	C2H2-type 1
	Zinc finger	342 – 366	25	C2H2-type 2
	Zinc finger	372 – 394	23	C2H2-type 3
	Zinc finger	400 – 422	23	C2H2-type 4
	Zinc finger	430 – 453	24	C2H2-type 5
	Region	338 – 368	31	Interaction with MAP3K14/NIK
	Compositional bias	21 – 67	47	Ala-rich
	Compositional bias	48 – 81	34	Arg-rich
	Compositional bias	197 – 282	86	Glu-rich
Amino acid modifications
	Modified residue	103	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	526 – 529	4	VSLM → SIHR in isoform 2.		VSP_012686
	Alternative sequence	530 – 570	41	Missing in isoform 2.		VSP_012687
	Natural variant	37	1	V → I. Ref.4 Corresponds to variant rs17854702 [ dbSNP | Ensembl ].		VAR_032454
	Natural variant	207	1	S → G. Ref.6 Corresponds to variant rs8373 [ dbSNP | Ensembl ].		VAR_021889
Experimental info
	Mutagenesis	344	1	C → A: Abolishes ubiquitination of MAP3K14/NIK; when associated with A-349. Ref.9
	Mutagenesis	349	1	C → A: Abolishes ubiquitination of MAP3K14/NIK; when associated with A-344. Ref.9
	Sequence conflict	74	1	A → V in AAL09963. Ref.6
	Sequence conflict	206	1	Missing in AAH51743. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 476F4C21DD453522	FASTA	570	63,445
	10 20 30 40 50 60 MPGETEEPRP PEQQDQEGGE AAKAAPEEPQ QRPPEAVAAA PAGTTSSRVL RGGRDRGRAA 70 80 90 100 110 120 AAAAAAAVSR RRKAEYPRRR RSSPSARPPD VPGQQPQAAK SPSPVQGKKS PRLLCIEKVT 130 140 150 160 170 180 TDKDPKEEKE EEDDSALPQE VSIAASRPSR GWRSSRTSVS RHRDTENTRS SRSKTGSLQL 190 200 210 220 230 240 ICKSEPNTDQ LDYDVGEEHQ SPGGISSEEE EEEEEEMLIS EEEIPFKDDP RDETYKPHLE 250 260 270 280 290 300 RETPKPRRKS GKVKEEKEKK EIKVEVEVEV KEEENEIRED EEPPRKRGRR RKDDKSPRLP 310 320 330 340 350 360 KRRKKPPIQY VRCEMEGCGT VLAHPRYLQH HIKYQHLLKK KYVCPHPSCG RLFRLQKQLL 370 380 390 400 410 420 RHAKHHTDQR DYICEYCARA FKSSHNLAVH RMIHTGEKPL QCEICGFTCR QKASLNWHMK 430 440 450 460 470 480 KHDADSFYQF SCNICGKKFE KKDSVVAHKA KSHPEVLIAE ALAANAGALI TSTDILGTNP 490 500 510 520 530 540 ESLTQPSDGQ GLPLLPEPLG NSTSGECLLL EAEGMSKSYC SGTERVSLMA DGKIFVGSGS 550 560 570 SGGTEGLVMN SDILGATTEV LIEDSDSAGP
	Isoform 2 [UniParc]. Checksum: D181F0420D5D004F	FASTA	529	59,571
	10 20 30 40 50 60 MPGETEEPRP PEQQDQEGGE AAKAAPEEPQ QRPPEAVAAA PAGTTSSRVL RGGRDRGRAA 70 80 90 100 110 120 AAAAAAAVSR RRKAEYPRRR RSSPSARPPD VPGQQPQAAK SPSPVQGKKS PRLLCIEKVT 130 140 150 160 170 180 TDKDPKEEKE EEDDSALPQE VSIAASRPSR GWRSSRTSVS RHRDTENTRS SRSKTGSLQL 190 200 210 220 230 240 ICKSEPNTDQ LDYDVGEEHQ SPGGISSEEE EEEEEEMLIS EEEIPFKDDP RDETYKPHLE 250 260 270 280 290 300 RETPKPRRKS GKVKEEKEKK EIKVEVEVEV KEEENEIRED EEPPRKRGRR RKDDKSPRLP 310 320 330 340 350 360 KRRKKPPIQY VRCEMEGCGT VLAHPRYLQH HIKYQHLLKK KYVCPHPSCG RLFRLQKQLL 370 380 390 400 410 420 RHAKHHTDQR DYICEYCARA FKSSHNLAVH RMIHTGEKPL QCEICGFTCR QKASLNWHMK 430 440 450 460 470 480 KHDADSFYQF SCNICGKKFE KKDSVVAHKA KSHPEVLIAE ALAANAGALI TSTDILGTNP 490 500 510 520 ESLTQPSDGQ GLPLLPEPLG NSTSGECLLL EAEGMSKSYC SGTERSIHR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel human gene, ZFP91, involved in acute myelogenous leukemia." Unoki M., Okutsu J., Nakamura Y. Int. J. Oncol. 22:1217-1223(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR LOCATION. Tissue: Leukocyte.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ILE-37. Tissue: Testis.
[5]	Bienvenut W.V., Waridel P., Quadroni M. Submitted (MAR-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-23, CLEAVAGE OF INITIATOR METHIONINE, LACK OF N-TERMINAL ACETYLATION, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Identification of FKSG11, a novel gene related to breast cancer." Wang Y.-G. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 67-570 (ISOFORM 1), VARIANT GLY-207.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"An atypical E3 ligase zinc finger protein 91 stabilizes and activates NF-kappaB-inducing kinase via Lys63-linked ubiquitination." Jin X., Jin H.R., Jung H.S., Lee S.J., Lee J.H., Lee J.J. J. Biol. Chem. 285:30539-30547(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MAP3K14, MUTAGENESIS OF CYS-344 AND CYS-349.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB056107 mRNA. Translation: BAB63373.1. AB057443 mRNA. Translation: BAB63374.1. AP001350 Genomic DNA. No translation available. CH471076 Genomic DNA. Translation: EAW73811.1. BC051743 mRNA. Translation: AAH51743.1. AF310246 mRNA. Translation: AAL09963.1. Frameshift.
IPI	IPI00172474. IPI00477661.
RefSeq	NP_001183980.1. NM_001197051.1. NP_444251.1. NM_053023.4.
UniGene	Hs.524920.
3D structure databases
ProteinModelPortal	Q96JP5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96JP5. 3 interactions.
STRING	9606.ENSP00000339030.
PTM databases
PhosphoSite	Q96JP5.
Polymorphism databases
DMDM	60416377.
Proteomic databases
PaxDb	Q96JP5.
PRIDE	Q96JP5.
Protocols and materials databases
DNASU	80829.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000316059; ENSP00000339030; ENSG00000186660.
GeneID	80829.
KEGG	hsa:80829.
UCSC	uc001nmx.4. human.
Organism-specific databases
CTD	80829.
GeneCards	GC11P058347.
HGNC	HGNC:14983. ZFP91.
HPA	CAB025417. HPA024037.
neXtProt	NX_Q96JP5.
PharmGKB	PA37955.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG290299.
HOGENOM	HOG000155779.
HOVERGEN	HBG055547.
InParanoid	Q96JP5.
OMA	SRPSRGW.
OrthoDB	EOG4Z8XXK.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q96JP5.
Bgee	Q96JP5.
CleanEx	HS_ZFP91.
Genevestigator	Q96JP5.
GermOnline	ENSG00000186660. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.60. 3 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
SMART	SM00355. ZnF_C2H2. 5 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 5 hits. PS50157. ZINC_FINGER_C2H2_2. 4 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ZFP91. human.
GenomeRNAi	80829.
NextBio	71236.

Entry information

Entry name

ZFP91_HUMAN

Accession

Primary (citable) accession number: Q96JP5
Secondary accession number(s): A6NHC4 , A8MSG7, Q86V47, Q96JP4, Q96QA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.