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Protein

E3 ubiquitin-protein ligase ZFP91

Gene

ZFP91

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Atypical E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabilize and activate MAP3K14/NIK. It thereby acts as an activator of the non-canonical NF-kappa-B2/NFKB2 pathway. May also play an important role in cell proliferation and/or anti-apoptosis.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri311 – 336C2H2-type 1PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri342 – 366C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri372 – 394C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri400 – 422C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri430 – 453C2H2-type 5PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

  • activation of NF-kappaB-inducing kinase activity Source: UniProtKB
  • protein K63-linked ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

SIGNORiQ96JP5.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase ZFP91 (EC:6.3.2.-)
Alternative name(s):
Zinc finger protein 757
Zinc finger protein 91 homolog
Short name:
Zfp-91
Gene namesi
Name:ZFP91
Synonyms:ZNF757
ORF Names:FKSG11
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:14983. ZFP91.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi344C → A: Abolishes ubiquitination of MAP3K14/NIK; when associated with A-349. 1 Publication1
Mutagenesisi349C → A: Abolishes ubiquitination of MAP3K14/NIK; when associated with A-344. 1 Publication1

Organism-specific databases

DisGeNETi80829.
OpenTargetsiENSG00000186660.
ENSG00000255073.
PharmGKBiPA37955.

Polymorphism and mutation databases

BioMutaiZFP91.
DMDMi60416377.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000473122 – 570E3 ubiquitin-protein ligase ZFP91Add BLAST569

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei83PhosphoserineBy similarity1
Modified residuei103PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ96JP5.
MaxQBiQ96JP5.
PaxDbiQ96JP5.
PeptideAtlasiQ96JP5.
PRIDEiQ96JP5.

PTM databases

iPTMnetiQ96JP5.
PhosphoSitePlusiQ96JP5.

Expressioni

Tissue specificityi

Expressed ubiquitously, particularly at high level in testis. Isoform 2 is testis specific.

Gene expression databases

BgeeiENSG00000186660.
CleanExiHS_ZFP91.
ExpressionAtlasiQ96JP5. baseline and differential.
GenevisibleiQ96JP5. HS.

Organism-specific databases

HPAiCAB025417.
HPA024037.
HPA065325.

Interactioni

Subunit structurei

Interacts with MAP3K14/NIK.2 Publications

Protein-protein interaction databases

BioGridi123322. 28 interactors.
IntActiQ96JP5. 13 interactors.
STRINGi9606.ENSP00000339030.

Structurei

Secondary structure

1570
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi381 – 383Combined sources3
Helixi384 – 390Combined sources7
Helixi391 – 393Combined sources3
Beta strandi395 – 397Combined sources3
Beta strandi408 – 411Combined sources4
Helixi412 – 423Combined sources12
Beta strandi426 – 429Combined sources4
Turni433 – 435Combined sources3
Helixi442 – 452Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2M9ANMR-A370-456[»]
ProteinModelPortaliQ96JP5.
SMRiQ96JP5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni338 – 368Interaction with MAP3K14/NIK1 PublicationAdd BLAST31

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi21 – 67Ala-richAdd BLAST47
Compositional biasi48 – 81Arg-richAdd BLAST34
Compositional biasi197 – 282Glu-richAdd BLAST86

Sequence similaritiesi

Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri311 – 336C2H2-type 1PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri342 – 366C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri372 – 394C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri400 – 422C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri430 – 453C2H2-type 5PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063153.
HOGENOMiHOG000155779.
HOVERGENiHBG055547.
InParanoidiQ96JP5.
OMAiVPFKDDP.
OrthoDBiEOG091G055J.
PhylomeDBiQ96JP5.
TreeFamiTF332664.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96JP5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPGETEEPRP PEQQDQEGGE AAKAAPEEPQ QRPPEAVAAA PAGTTSSRVL
60 70 80 90 100
RGGRDRGRAA AAAAAAAVSR RRKAEYPRRR RSSPSARPPD VPGQQPQAAK
110 120 130 140 150
SPSPVQGKKS PRLLCIEKVT TDKDPKEEKE EEDDSALPQE VSIAASRPSR
160 170 180 190 200
GWRSSRTSVS RHRDTENTRS SRSKTGSLQL ICKSEPNTDQ LDYDVGEEHQ
210 220 230 240 250
SPGGISSEEE EEEEEEMLIS EEEIPFKDDP RDETYKPHLE RETPKPRRKS
260 270 280 290 300
GKVKEEKEKK EIKVEVEVEV KEEENEIRED EEPPRKRGRR RKDDKSPRLP
310 320 330 340 350
KRRKKPPIQY VRCEMEGCGT VLAHPRYLQH HIKYQHLLKK KYVCPHPSCG
360 370 380 390 400
RLFRLQKQLL RHAKHHTDQR DYICEYCARA FKSSHNLAVH RMIHTGEKPL
410 420 430 440 450
QCEICGFTCR QKASLNWHMK KHDADSFYQF SCNICGKKFE KKDSVVAHKA
460 470 480 490 500
KSHPEVLIAE ALAANAGALI TSTDILGTNP ESLTQPSDGQ GLPLLPEPLG
510 520 530 540 550
NSTSGECLLL EAEGMSKSYC SGTERVSLMA DGKIFVGSGS SGGTEGLVMN
560 570
SDILGATTEV LIEDSDSAGP
Length:570
Mass (Da):63,445
Last modified:December 1, 2001 - v1
Checksum:i476F4C21DD453522
GO
Isoform 2 (identifier: Q96JP5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     526-529: VSLM → SIHR
     530-570: Missing.

Show »
Length:529
Mass (Da):59,571
Checksum:iD181F0420D5D004F
GO

Sequence cautioni

The sequence AAL09963 differs from that shown. Reason: Frameshift at positions 72 and 80.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74A → V in AAL09963 (Ref. 6) Curated1
Sequence conflicti206Missing in AAH51743 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03245437V → I.1 PublicationCorresponds to variant rs17854702dbSNPEnsembl.1
Natural variantiVAR_021889207S → G.1 PublicationCorresponds to variant rs8373dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012686526 – 529VSLM → SIHR in isoform 2. 1 Publication4
Alternative sequenceiVSP_012687530 – 570Missing in isoform 2. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056107 mRNA. Translation: BAB63373.1.
AB057443 mRNA. Translation: BAB63374.1.
AP001350 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73811.1.
BC051743 mRNA. Translation: AAH51743.1.
AF310246 mRNA. Translation: AAL09963.1. Frameshift.
CCDSiCCDS31553.1. [Q96JP5-1]
RefSeqiNP_001183980.1. NM_001197051.1.
NP_444251.1. NM_053023.4. [Q96JP5-1]
UniGeneiHs.524920.

Genome annotation databases

EnsembliENST00000316059; ENSP00000339030; ENSG00000186660. [Q96JP5-1]
GeneIDi80829.
KEGGihsa:80829.
UCSCiuc001nmx.5. human. [Q96JP5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056107 mRNA. Translation: BAB63373.1.
AB057443 mRNA. Translation: BAB63374.1.
AP001350 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73811.1.
BC051743 mRNA. Translation: AAH51743.1.
AF310246 mRNA. Translation: AAL09963.1. Frameshift.
CCDSiCCDS31553.1. [Q96JP5-1]
RefSeqiNP_001183980.1. NM_001197051.1.
NP_444251.1. NM_053023.4. [Q96JP5-1]
UniGeneiHs.524920.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2M9ANMR-A370-456[»]
ProteinModelPortaliQ96JP5.
SMRiQ96JP5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123322. 28 interactors.
IntActiQ96JP5. 13 interactors.
STRINGi9606.ENSP00000339030.

PTM databases

iPTMnetiQ96JP5.
PhosphoSitePlusiQ96JP5.

Polymorphism and mutation databases

BioMutaiZFP91.
DMDMi60416377.

Proteomic databases

EPDiQ96JP5.
MaxQBiQ96JP5.
PaxDbiQ96JP5.
PeptideAtlasiQ96JP5.
PRIDEiQ96JP5.

Protocols and materials databases

DNASUi80829.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000316059; ENSP00000339030; ENSG00000186660. [Q96JP5-1]
GeneIDi80829.
KEGGihsa:80829.
UCSCiuc001nmx.5. human. [Q96JP5-1]

Organism-specific databases

CTDi80829.
DisGeNETi80829.
GeneCardsiZFP91.
HGNCiHGNC:14983. ZFP91.
HPAiCAB025417.
HPA024037.
HPA065325.
neXtProtiNX_Q96JP5.
OpenTargetsiENSG00000186660.
ENSG00000255073.
PharmGKBiPA37955.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063153.
HOGENOMiHOG000155779.
HOVERGENiHBG055547.
InParanoidiQ96JP5.
OMAiVPFKDDP.
OrthoDBiEOG091G055J.
PhylomeDBiQ96JP5.
TreeFamiTF332664.

Enzyme and pathway databases

UniPathwayiUPA00143.
SIGNORiQ96JP5.

Miscellaneous databases

ChiTaRSiZFP91. human.
GeneWikiiZFP91.
GenomeRNAii80829.
PROiQ96JP5.

Gene expression databases

BgeeiENSG00000186660.
CleanExiHS_ZFP91.
ExpressionAtlasiQ96JP5. baseline and differential.
GenevisibleiQ96JP5. HS.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZFP91_HUMAN
AccessioniPrimary (citable) accession number: Q96JP5
Secondary accession number(s): A6NHC4
, A8MSG7, Q86V47, Q96JP4, Q96QA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In contrast to other E3 ubiquitin-protein ligase, does not contain any domain (RING-type zinc finger or HECT domain) known to mediate E3 ligase activity.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.