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Q96JP5 (ZFP91_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase ZFP91

EC=6.3.2.-
Alternative name(s):
Zinc finger protein 757
Zinc finger protein 91 homolog
Short name=Zfp-91
Gene names
Name:ZFP91
Synonyms:ZNF757
ORF Names:FKSG11
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length570 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Atypical E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabilize and activate MAP3K14/NIK. It thereby acts as an activator of the non-canonical NF-kappa-B2/NFKB2 pathway. May also play an important role in cell proliferation and/or anti-apoptosis. Ref.1 Ref.9

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with MAP3K14/NIK. Ref.9

Subcellular location

Nucleus Ref.1.

Tissue specificity

Expressed ubiquitously, particularly at high level in testis. Isoform 2 is testis specific.

Miscellaneous

In contrast to other E3 ubiquitin-protein ligase, does not contain any domain (RING-type zinc finger or HECT domain) known to mediate E3 ligase activity.

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Contains 5 C2H2-type zinc fingers.

Sequence caution

The sequence AAL09963.1 differs from that shown. Reason: Frameshift at positions 72 and 80.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionLigase
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of NF-kappaB-inducing kinase activity

Inferred from direct assay Ref.9. Source: UniProtKB

protein K63-linked ubiquitination

Inferred from direct assay Ref.9. Source: UniProtKB

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

ubiquitin-protein ligase activity

Inferred from direct assay Ref.9. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96JP5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96JP5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     526-529: VSLM → SIHR
     530-570: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 570569E3 ubiquitin-protein ligase ZFP91
PRO_0000047312

Regions

Zinc finger311 – 33626C2H2-type 1
Zinc finger342 – 36625C2H2-type 2
Zinc finger372 – 39423C2H2-type 3
Zinc finger400 – 42223C2H2-type 4
Zinc finger430 – 45324C2H2-type 5
Region338 – 36831Interaction with MAP3K14/NIK
Compositional bias21 – 6747Ala-rich
Compositional bias48 – 8134Arg-rich
Compositional bias197 – 28286Glu-rich

Amino acid modifications

Modified residue1031Phosphoserine By similarity

Natural variations

Alternative sequence526 – 5294VSLM → SIHR in isoform 2.
VSP_012686
Alternative sequence530 – 57041Missing in isoform 2.
VSP_012687
Natural variant371V → I. Ref.4
Corresponds to variant rs17854702 [ dbSNP | Ensembl ].
VAR_032454
Natural variant2071S → G. Ref.6
Corresponds to variant rs8373 [ dbSNP | Ensembl ].
VAR_021889

Experimental info

Mutagenesis3441C → A: Abolishes ubiquitination of MAP3K14/NIK; when associated with A-349. Ref.9
Mutagenesis3491C → A: Abolishes ubiquitination of MAP3K14/NIK; when associated with A-344. Ref.9
Sequence conflict741A → V in AAL09963. Ref.6
Sequence conflict2061Missing in AAH51743. Ref.4

Secondary structure

................ 570
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 476F4C21DD453522

FASTA57063,445
        10         20         30         40         50         60 
MPGETEEPRP PEQQDQEGGE AAKAAPEEPQ QRPPEAVAAA PAGTTSSRVL RGGRDRGRAA 

        70         80         90        100        110        120 
AAAAAAAVSR RRKAEYPRRR RSSPSARPPD VPGQQPQAAK SPSPVQGKKS PRLLCIEKVT 

       130        140        150        160        170        180 
TDKDPKEEKE EEDDSALPQE VSIAASRPSR GWRSSRTSVS RHRDTENTRS SRSKTGSLQL 

       190        200        210        220        230        240 
ICKSEPNTDQ LDYDVGEEHQ SPGGISSEEE EEEEEEMLIS EEEIPFKDDP RDETYKPHLE 

       250        260        270        280        290        300 
RETPKPRRKS GKVKEEKEKK EIKVEVEVEV KEEENEIRED EEPPRKRGRR RKDDKSPRLP 

       310        320        330        340        350        360 
KRRKKPPIQY VRCEMEGCGT VLAHPRYLQH HIKYQHLLKK KYVCPHPSCG RLFRLQKQLL 

       370        380        390        400        410        420 
RHAKHHTDQR DYICEYCARA FKSSHNLAVH RMIHTGEKPL QCEICGFTCR QKASLNWHMK 

       430        440        450        460        470        480 
KHDADSFYQF SCNICGKKFE KKDSVVAHKA KSHPEVLIAE ALAANAGALI TSTDILGTNP 

       490        500        510        520        530        540 
ESLTQPSDGQ GLPLLPEPLG NSTSGECLLL EAEGMSKSYC SGTERVSLMA DGKIFVGSGS 

       550        560        570 
SGGTEGLVMN SDILGATTEV LIEDSDSAGP 

« Hide

Isoform 2 [UniParc].

Checksum: D181F0420D5D004F
Show »

FASTA52959,571

References

« Hide 'large scale' references
[1]"Identification of a novel human gene, ZFP91, involved in acute myelogenous leukemia."
Unoki M., Okutsu J., Nakamura Y.
Int. J. Oncol. 22:1217-1223(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR LOCATION.
Tissue: Leukocyte.
[2]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ILE-37.
Tissue: Testis.
[5]Bienvenut W.V., Waridel P., Quadroni M.
Submitted (MAR-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-23, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[6]"Identification of FKSG11, a novel gene related to breast cancer."
Wang Y.-G.
Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 67-570 (ISOFORM 1), VARIANT GLY-207.
[7]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"An atypical E3 ligase zinc finger protein 91 stabilizes and activates NF-kappaB-inducing kinase via Lys63-linked ubiquitination."
Jin X., Jin H.R., Jung H.S., Lee S.J., Lee J.H., Lee J.J.
J. Biol. Chem. 285:30539-30547(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MAP3K14, MUTAGENESIS OF CYS-344 AND CYS-349.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Solution structure of HR7784A."
Northeast structural genomics consortium (NESG)
Submitted (JUL-2013) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 370-456 IN COMPLEX WITH ZINC IONS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB056107 mRNA. Translation: BAB63373.1.
AB057443 mRNA. Translation: BAB63374.1.
AP001350 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73811.1.
BC051743 mRNA. Translation: AAH51743.1.
AF310246 mRNA. Translation: AAL09963.1. Frameshift.
RefSeqNP_001183980.1. NM_001197051.1.
NP_444251.1. NM_053023.4.
UniGeneHs.524920.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2M9ANMR-A370-456[»]
ProteinModelPortalQ96JP5.
SMRQ96JP5. Positions 313-456.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123322. 9 interactions.
IntActQ96JP5. 3 interactions.
STRING9606.ENSP00000339030.

PTM databases

PhosphoSiteQ96JP5.

Polymorphism databases

DMDM60416377.

Proteomic databases

PaxDbQ96JP5.
PRIDEQ96JP5.

Protocols and materials databases

DNASU80829.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000316059; ENSP00000339030; ENSG00000186660. [Q96JP5-1]
GeneID80829.
KEGGhsa:80829.
UCSCuc001nmx.4. human. [Q96JP5-1]

Organism-specific databases

CTD80829.
GeneCardsGC11P058347.
HGNCHGNC:14983. ZFP91.
HPACAB025417.
HPA024037.
neXtProtNX_Q96JP5.
PharmGKBPA37955.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG290299.
HOGENOMHOG000155779.
HOVERGENHBG055547.
InParanoidQ96JP5.
OMAGLVMNTE.
OrthoDBEOG7SV0TZ.
PhylomeDBQ96JP5.
TreeFamTF332664.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ96JP5.
BgeeQ96JP5.
CleanExHS_ZFP91.
GenevestigatorQ96JP5.

Family and domain databases

Gene3D3.30.160.60. 3 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZFP91. human.
GeneWikiZFP91.
GenomeRNAi80829.
NextBio71236.
PROQ96JP5.

Entry information

Entry nameZFP91_HUMAN
AccessionPrimary (citable) accession number: Q96JP5
Secondary accession number(s): A6NHC4 expand/collapse secondary AC list , A8MSG7, Q86V47, Q96JP4, Q96QA3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM