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Protein

E3 ubiquitin-protein ligase ZFP91

Gene

ZFP91

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Atypical E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabilize and activate MAP3K14/NIK. It thereby acts as an activator of the non-canonical NF-kappa-B2/NFKB2 pathway. May also play an important role in cell proliferation and/or anti-apoptosis.2 Publications

Pathway:iprotein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri311 – 33626C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri342 – 36625C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri372 – 39423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri400 – 42223C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri430 – 45324C2H2-type 5PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • activation of NF-kappaB-inducing kinase activity Source: UniProtKB
  • protein K63-linked ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase ZFP91 (EC:6.3.2.-)
Alternative name(s):
Zinc finger protein 757
Zinc finger protein 91 homolog
Short name:
Zfp-91
Gene namesi
Name:ZFP91
Synonyms:ZNF757
ORF Names:FKSG11
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:14983. ZFP91.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi344 – 3441C → A: Abolishes ubiquitination of MAP3K14/NIK; when associated with A-349. 1 Publication
Mutagenesisi349 – 3491C → A: Abolishes ubiquitination of MAP3K14/NIK; when associated with A-344. 1 Publication

Organism-specific databases

PharmGKBiPA37955.

Polymorphism and mutation databases

BioMutaiZFP91.
DMDMi60416377.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 570569E3 ubiquitin-protein ligase ZFP91PRO_0000047312Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei103 – 1031PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ96JP5.
PaxDbiQ96JP5.
PRIDEiQ96JP5.

PTM databases

PhosphoSiteiQ96JP5.

Expressioni

Tissue specificityi

Expressed ubiquitously, particularly at high level in testis. Isoform 2 is testis specific.

Gene expression databases

BgeeiQ96JP5.
CleanExiHS_ZFP91.
ExpressionAtlasiQ96JP5. baseline and differential.
GenevisibleiQ96JP5. HS.

Organism-specific databases

HPAiCAB025417.
HPA024037.

Interactioni

Subunit structurei

Interacts with MAP3K14/NIK.2 Publications

Protein-protein interaction databases

BioGridi123322. 10 interactions.
IntActiQ96JP5. 3 interactions.
STRINGi9606.ENSP00000339030.

Structurei

Secondary structure

1
570
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi381 – 3833Combined sources
Helixi384 – 3907Combined sources
Helixi391 – 3933Combined sources
Beta strandi395 – 3973Combined sources
Beta strandi408 – 4114Combined sources
Helixi412 – 42312Combined sources
Beta strandi426 – 4294Combined sources
Turni433 – 4353Combined sources
Helixi442 – 45211Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2M9ANMR-A370-456[»]
ProteinModelPortaliQ96JP5.
SMRiQ96JP5. Positions 313-456.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni338 – 36831Interaction with MAP3K14/NIKAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi21 – 6747Ala-richAdd
BLAST
Compositional biasi48 – 8134Arg-richAdd
BLAST
Compositional biasi197 – 28286Glu-richAdd
BLAST

Sequence similaritiesi

Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri311 – 33626C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri342 – 36625C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri372 – 39423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri400 – 42223C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri430 – 45324C2H2-type 5PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG290299.
GeneTreeiENSGT00530000063153.
HOGENOMiHOG000155779.
HOVERGENiHBG055547.
InParanoidiQ96JP5.
OMAiVPFKDDP.
OrthoDBiEOG7SV0TZ.
PhylomeDBiQ96JP5.
TreeFamiTF332664.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96JP5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPGETEEPRP PEQQDQEGGE AAKAAPEEPQ QRPPEAVAAA PAGTTSSRVL
60 70 80 90 100
RGGRDRGRAA AAAAAAAVSR RRKAEYPRRR RSSPSARPPD VPGQQPQAAK
110 120 130 140 150
SPSPVQGKKS PRLLCIEKVT TDKDPKEEKE EEDDSALPQE VSIAASRPSR
160 170 180 190 200
GWRSSRTSVS RHRDTENTRS SRSKTGSLQL ICKSEPNTDQ LDYDVGEEHQ
210 220 230 240 250
SPGGISSEEE EEEEEEMLIS EEEIPFKDDP RDETYKPHLE RETPKPRRKS
260 270 280 290 300
GKVKEEKEKK EIKVEVEVEV KEEENEIRED EEPPRKRGRR RKDDKSPRLP
310 320 330 340 350
KRRKKPPIQY VRCEMEGCGT VLAHPRYLQH HIKYQHLLKK KYVCPHPSCG
360 370 380 390 400
RLFRLQKQLL RHAKHHTDQR DYICEYCARA FKSSHNLAVH RMIHTGEKPL
410 420 430 440 450
QCEICGFTCR QKASLNWHMK KHDADSFYQF SCNICGKKFE KKDSVVAHKA
460 470 480 490 500
KSHPEVLIAE ALAANAGALI TSTDILGTNP ESLTQPSDGQ GLPLLPEPLG
510 520 530 540 550
NSTSGECLLL EAEGMSKSYC SGTERVSLMA DGKIFVGSGS SGGTEGLVMN
560 570
SDILGATTEV LIEDSDSAGP
Length:570
Mass (Da):63,445
Last modified:December 1, 2001 - v1
Checksum:i476F4C21DD453522
GO
Isoform 2 (identifier: Q96JP5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     526-529: VSLM → SIHR
     530-570: Missing.

Show »
Length:529
Mass (Da):59,571
Checksum:iD181F0420D5D004F
GO

Sequence cautioni

The sequence AAL09963.1 differs from that shown. Reason: Frameshift at positions 72 and 80. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 741A → V in AAL09963 (Ref. 6) Curated
Sequence conflicti206 – 2061Missing in AAH51743 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti37 – 371V → I.1 Publication
Corresponds to variant rs17854702 [ dbSNP | Ensembl ].
VAR_032454
Natural varianti207 – 2071S → G.1 Publication
Corresponds to variant rs8373 [ dbSNP | Ensembl ].
VAR_021889

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei526 – 5294VSLM → SIHR in isoform 2. 1 PublicationVSP_012686
Alternative sequencei530 – 57041Missing in isoform 2. 1 PublicationVSP_012687Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056107 mRNA. Translation: BAB63373.1.
AB057443 mRNA. Translation: BAB63374.1.
AP001350 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73811.1.
BC051743 mRNA. Translation: AAH51743.1.
AF310246 mRNA. Translation: AAL09963.1. Frameshift.
CCDSiCCDS31553.1. [Q96JP5-1]
RefSeqiNP_001183980.1. NM_001197051.1.
NP_444251.1. NM_053023.4. [Q96JP5-1]
UniGeneiHs.524920.

Genome annotation databases

EnsembliENST00000316059; ENSP00000339030; ENSG00000186660.
GeneIDi80829.
KEGGihsa:80829.
UCSCiuc001nmx.4. human. [Q96JP5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056107 mRNA. Translation: BAB63373.1.
AB057443 mRNA. Translation: BAB63374.1.
AP001350 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73811.1.
BC051743 mRNA. Translation: AAH51743.1.
AF310246 mRNA. Translation: AAL09963.1. Frameshift.
CCDSiCCDS31553.1. [Q96JP5-1]
RefSeqiNP_001183980.1. NM_001197051.1.
NP_444251.1. NM_053023.4. [Q96JP5-1]
UniGeneiHs.524920.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2M9ANMR-A370-456[»]
ProteinModelPortaliQ96JP5.
SMRiQ96JP5. Positions 313-456.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123322. 10 interactions.
IntActiQ96JP5. 3 interactions.
STRINGi9606.ENSP00000339030.

PTM databases

PhosphoSiteiQ96JP5.

Polymorphism and mutation databases

BioMutaiZFP91.
DMDMi60416377.

Proteomic databases

MaxQBiQ96JP5.
PaxDbiQ96JP5.
PRIDEiQ96JP5.

Protocols and materials databases

DNASUi80829.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000316059; ENSP00000339030; ENSG00000186660.
GeneIDi80829.
KEGGihsa:80829.
UCSCiuc001nmx.4. human. [Q96JP5-1]

Organism-specific databases

CTDi80829.
GeneCardsiGC11P058347.
HGNCiHGNC:14983. ZFP91.
HPAiCAB025417.
HPA024037.
neXtProtiNX_Q96JP5.
PharmGKBiPA37955.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG290299.
GeneTreeiENSGT00530000063153.
HOGENOMiHOG000155779.
HOVERGENiHBG055547.
InParanoidiQ96JP5.
OMAiVPFKDDP.
OrthoDBiEOG7SV0TZ.
PhylomeDBiQ96JP5.
TreeFamiTF332664.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiZFP91. human.
GeneWikiiZFP91.
GenomeRNAii80829.
NextBioi71236.
PROiQ96JP5.

Gene expression databases

BgeeiQ96JP5.
CleanExiHS_ZFP91.
ExpressionAtlasiQ96JP5. baseline and differential.
GenevisibleiQ96JP5. HS.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel human gene, ZFP91, involved in acute myelogenous leukemia."
    Unoki M., Okutsu J., Nakamura Y.
    Int. J. Oncol. 22:1217-1223(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR LOCATION.
    Tissue: Leukocyte.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ILE-37.
    Tissue: Testis.
  5. Bienvenut W.V., Waridel P., Quadroni M.
    Submitted (MAR-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-23, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Cervix carcinoma.
  6. "Identification of FKSG11, a novel gene related to breast cancer."
    Wang Y.-G.
    Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 67-570 (ISOFORM 1), VARIANT GLY-207.
  7. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "An atypical E3 ligase zinc finger protein 91 stabilizes and activates NF-kappaB-inducing kinase via Lys63-linked ubiquitination."
    Jin X., Jin H.R., Jung H.S., Lee S.J., Lee J.H., Lee J.J.
    J. Biol. Chem. 285:30539-30547(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MAP3K14, MUTAGENESIS OF CYS-344 AND CYS-349.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  12. "Solution structure of HR7784A."
    Northeast structural genomics consortium (NESG)
    Submitted (JUL-2013) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 370-456 IN COMPLEX WITH ZINC IONS.

Entry informationi

Entry nameiZFP91_HUMAN
AccessioniPrimary (citable) accession number: Q96JP5
Secondary accession number(s): A6NHC4
, A8MSG7, Q86V47, Q96JP4, Q96QA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: December 1, 2001
Last modified: July 22, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In contrast to other E3 ubiquitin-protein ligase, does not contain any domain (RING-type zinc finger or HECT domain) known to mediate E3 ligase activity.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.