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Protein

Neuralized-like protein 4

Gene

NEURL4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Promotes CCP110 ubiquitination and proteasome-dependent degradation. By counteracting accumulation of CP110, maintains normal centriolar homeostasis and preventing formation of ectopic microtubular organizing centers.2 Publications

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Neuralized-like protein 4
Gene namesi
Name:NEURL4
Synonyms:KIAA1787
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:34410. NEURL4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164723835.

Polymorphism and mutation databases

BioMutaiNEURL4.
DMDMi156630938.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15621562Neuralized-like protein 4PRO_0000299105Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei502 – 5021PhosphoserineCombined sources
Modified residuei907 – 9071PhosphoserineCombined sources

Post-translational modificationi

Ubiquitinated; undergoes HERC2-dependent 'Lys-48' ubiquitination. This ubiquitination leads to proteasomal degradation.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ96JN8.
MaxQBiQ96JN8.
PaxDbiQ96JN8.
PeptideAtlasiQ96JN8.
PRIDEiQ96JN8.

PTM databases

iPTMnetiQ96JN8.
PhosphoSiteiQ96JN8.

Expressioni

Tissue specificityi

Widely expressed at high levels (including brain).1 Publication

Gene expression databases

BgeeiQ96JN8.
ExpressionAtlasiQ96JN8. baseline and differential.
GenevisibleiQ96JN8. HS.

Organism-specific databases

HPAiHPA043678.
HPA055314.

Interactioni

Subunit structurei

Interacts with CCP110; this interaction propmotes CCP110 ubiquitination and degradation via the proteasome pathway. Via its interaction with CCP110, may indirectly interact with CEP97. Interacts with the E3 ubiquitin-protein ligase HERC2 and UBE3A. May interact with MAPK6 and hence mediate MAPK6 interaction with UBE3A. Interaction with UBE3A may be indirect and mediated by HERC2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CCP110O433039EBI-1053406,EBI-1566217

Protein-protein interaction databases

BioGridi124095. 61 interactions.
IntActiQ96JN8. 13 interactions.
MINTiMINT-8392025.
STRINGi9606.ENSP00000382390.

Structurei

Secondary structure

1
1562
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi50 – 545Combined sources
Beta strandi56 – 583Combined sources
Beta strandi60 – 645Combined sources
Turni66 – 683Combined sources
Beta strandi86 – 949Combined sources
Beta strandi103 – 1097Combined sources
Helixi120 – 1223Combined sources
Beta strandi125 – 13915Combined sources
Beta strandi141 – 1455Combined sources
Helixi150 – 1523Combined sources
Beta strandi159 – 1635Combined sources
Beta strandi169 – 1757Combined sources
Beta strandi177 – 1826Combined sources
Beta strandi189 – 1946Combined sources
Beta strandi199 – 2046Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E63NMR-A43-205[»]
ProteinModelPortaliQ96JN8.
SMRiQ96JN8. Positions 36-205, 320-482, 523-684, 711-881, 910-1084, 1139-1292.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96JN8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 207167NHR 1PROSITE-ProRule annotationAdd
BLAST
Domaini317 – 484168NHR 2PROSITE-ProRule annotationAdd
BLAST
Domaini520 – 686167NHR 3PROSITE-ProRule annotationAdd
BLAST
Domaini716 – 884169NHR 4PROSITE-ProRule annotationAdd
BLAST
Domaini913 – 1086174NHR 5PROSITE-ProRule annotationAdd
BLAST
Domaini1131 – 1294164NHR 6PROSITE-ProRule annotationAdd
BLAST

Domaini

The third NHR domain (NHR 3) is required for localization to both mother and daughter centrioles. NHR 1 restricts targeting to daughter centriole (PubMed:22441691). NHR 3 and 4 are required for CCP110/CEP97-binding, but not for HERC2-binding. NHR 5 and 6 are important for HERC2-binding and centrosomal localization (PubMed:22261722).2 Publications

Sequence similaritiesi

Contains 6 NHR (neuralized homology repeat) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IMDW. Eukaryota.
ENOG410XT3C. LUCA.
GeneTreeiENSGT00730000110931.
HOGENOMiHOG000007538.
InParanoidiQ96JN8.
KOiK16777.
OMAiGKCTQVT.
OrthoDBiEOG7XWPMQ.
PhylomeDBiQ96JN8.

Family and domain databases

InterProiIPR013320. ConA-like_dom.
IPR006573. NHR_dom.
[Graphical view]
PfamiPF07177. Neuralized. 6 hits.
[Graphical view]
SMARTiSM00588. NEUZ. 6 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiPS51065. NHR. 6 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96JN8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGSGGSGG SGGGPGPGPG GGGGPSGSGS GPGSNGGLGS GGELHPRTGR
60 70 80 90 100
LVSLSACGRT ARRQQPGQEF NHGLVLSREP LRDGRVFTVR IDRKVNSWSG
110 120 130 140 150
SIEIGVTALD PSVLDFPSSA TGLKGGSWVV SGCSVLRDGR SVLEEYGQDL
160 170 180 190 200
DQLGEGDRVG VERTVAGELR LWVNGRDCGV AATGLPPRVW AVVDLYGKCT
210 220 230 240 250
QITVLPPEPG FSPPTPIPTP PLEPLAPTED SALAEQGTSA DEAFMVSPAQ
260 270 280 290 300
ARPETFPNSL ESHNDFANME LSEVVSNTIL SAYNGGLLNV NLSSPPAGEG
310 320 330 340 350
LGSSGAATSP ILTSNDALLF HEKCGTLIKL SNNNKTAERR RPLDEFNNGV
360 370 380 390 400
VMTNRPLRDN EMFEIRIDKL VDKWSGSIEI GVTTHNPNSL EYPATMTNLQ
410 420 430 440 450
SGTIMMSGCG ILTNGKGTRR EYCEFSLDEL QEGDHIGLTR KSNSALHFFI
460 470 480 490 500
NGIDQGVATP LTPPVVYGVV DLYGMAVKVT IVHNNNHSDR LRRNNAILRA
510 520 530 540 550
LSPEGALRRA APAAQAEPER LLFHPNCGQK AAITHEGRTA LRPHATDDFN
560 570 580 590 600
HGVVLSSRAL RDGEVFQVRI DKMVDKWAGS IEIGVTTHNP AYLQLPSTMT
610 620 630 640 650
NLRSGTWMMT GNGVMHNGTT ILDEYGHNLD RLKAGDTVGV VRREDGTLHF
660 670 680 690 700
FVNGMTQGPA AWNVPPGVYA VVDLYGQAAQ ATIVDDVEVA PVPEPLPEGN
710 720 730 740 750
NQVSPSSPSS GAGGSDLRFH QLHGSNAVIT NGGRTALRHN CRSEFNDAIV
760 770 780 790 800
ISNRALRDGE LFEIVIQKMV DRWSGSIEAG VTAIRPEDLE FPNTMTDIDY
810 820 830 840 850
DTWMLSGTAI MQDGNTMRNN YGCDLDALGT GARIGMMRTA KGDLHYFING
860 870 880 890 900
QDQGAACSGL PPGKEVYAVV DLYGQCVQVS ITNATGPMDN SLATSNTATE
910 920 930 940 950
KSFPLHSPVA GVAHRFHSTC GKNVTLEEDG TRAVRAAGYA HGLVFSTKEL
960 970 980 990 1000
RAEEVFEVKV EELDEKWAGS LRLGLTTLAP GEMGPGAGGG GPGLPPSLPE
1010 1020 1030 1040 1050
LRTKTTWMVS SCEVRRDGQL QRMNYGRNLE RLGVGSRVGV RRGADDTMHI
1060 1070 1080 1090 1100
LVDGEDMGPA ATGIAKNVWA VLDLYGPVRG VSIVSSTRLE ESEGTQPPSP
1110 1120 1130 1140 1150
SSDTGSEGEE DDEGEEHGLG GQNEVGIIPT TLEFLENHGK NILLSNGNRT
1160 1170 1180 1190 1200
ATRVASYNQG IVVINQPLVP QLLVQVRIDF LNRQWTSSLV LGVITCAPER
1210 1220 1230 1240 1250
LNFPASACAL KRAAWLLRGR GVFHNGLKIC EKFGPNLDTC PEGTILGLRL
1260 1270 1280 1290 1300
DSSGGLHLHV NGVDQGVAVP DVPQPCHALV DLYGQCEQVT IVNPEPGAAS
1310 1320 1330 1340 1350
GKSAGTQGDM EKADMVDGIK ESVCWGPPPA ASPLKSCEYH ALCSRFQELL
1360 1370 1380 1390 1400
LLPEDYFMPP PKRSLCYCES CRKLRGDEAH RRRGEPPREY ALPFGWCRFN
1410 1420 1430 1440 1450
LRVNPRLEAG TLTKKWHMAY HGSNVAAVRR VLDRGELGAG TASILSCRPL
1460 1470 1480 1490 1500
KGEPGVGFEE PGENCAPPRE EQPPPVLLSP SLQYAGAETL ASKVQFRDPK
1510 1520 1530 1540 1550
SQRTHQAQVA FQVCVRPGSY TPGPPSAALG EPPDPHFSPA ELEWVTKEKG
1560
ATLLCALLVR VE
Length:1,562
Mass (Da):166,907
Last modified:August 21, 2007 - v2
Checksum:iCB25753A5D127246
GO
Isoform 2 (identifier: Q96JN8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     863-864: Missing.

Show »
Length:1,560
Mass (Da):166,722
Checksum:i7DE44AED5D555460
GO

Sequence cautioni

The sequence AAH07227.1 differs from that shown. Reason: Frameshift at position 1263. Curated
The sequence BAB47416.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti228 – 2281T → S in CAB66804 (PubMed:11230166).Curated
Sequence conflicti359 – 3591D → G in CAB66804 (PubMed:11230166).Curated
Sequence conflicti637 – 6371T → A in CAB66804 (PubMed:11230166).Curated
Sequence conflicti1262 – 12621G → A in AAH07227 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti1019 – 10191Q → H.1 Publication
Corresponds to variant rs3809813 [ dbSNP | Ensembl ].
VAR_049527

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei863 – 8642Missing in isoform 2. 2 PublicationsVSP_027563

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058690 mRNA. Translation: BAB47416.1. Different initiation.
AL136870 mRNA. Translation: CAB66804.1.
BC007227 mRNA. Translation: AAH07227.1. Frameshift.
BC073134 mRNA. Translation: AAH73134.1.
CCDSiCCDS42251.1. [Q96JN8-1]
CCDS42252.1. [Q96JN8-2]
RefSeqiNP_001005408.1. NM_001005408.1. [Q96JN8-2]
NP_115818.2. NM_032442.2. [Q96JN8-1]
UniGeneiHs.654794.

Genome annotation databases

EnsembliENST00000315614; ENSP00000319826; ENSG00000215041. [Q96JN8-2]
ENST00000399464; ENSP00000382390; ENSG00000215041. [Q96JN8-1]
GeneIDi84461.
KEGGihsa:84461.
UCSCiuc002gga.2. human. [Q96JN8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058690 mRNA. Translation: BAB47416.1. Different initiation.
AL136870 mRNA. Translation: CAB66804.1.
BC007227 mRNA. Translation: AAH07227.1. Frameshift.
BC073134 mRNA. Translation: AAH73134.1.
CCDSiCCDS42251.1. [Q96JN8-1]
CCDS42252.1. [Q96JN8-2]
RefSeqiNP_001005408.1. NM_001005408.1. [Q96JN8-2]
NP_115818.2. NM_032442.2. [Q96JN8-1]
UniGeneiHs.654794.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E63NMR-A43-205[»]
ProteinModelPortaliQ96JN8.
SMRiQ96JN8. Positions 36-205, 320-482, 523-684, 711-881, 910-1084, 1139-1292.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124095. 61 interactions.
IntActiQ96JN8. 13 interactions.
MINTiMINT-8392025.
STRINGi9606.ENSP00000382390.

PTM databases

iPTMnetiQ96JN8.
PhosphoSiteiQ96JN8.

Polymorphism and mutation databases

BioMutaiNEURL4.
DMDMi156630938.

Proteomic databases

EPDiQ96JN8.
MaxQBiQ96JN8.
PaxDbiQ96JN8.
PeptideAtlasiQ96JN8.
PRIDEiQ96JN8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000315614; ENSP00000319826; ENSG00000215041. [Q96JN8-2]
ENST00000399464; ENSP00000382390; ENSG00000215041. [Q96JN8-1]
GeneIDi84461.
KEGGihsa:84461.
UCSCiuc002gga.2. human. [Q96JN8-1]

Organism-specific databases

CTDi84461.
GeneCardsiNEURL4.
HGNCiHGNC:34410. NEURL4.
HPAiHPA043678.
HPA055314.
MIMi615865. gene.
neXtProtiNX_Q96JN8.
PharmGKBiPA164723835.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IMDW. Eukaryota.
ENOG410XT3C. LUCA.
GeneTreeiENSGT00730000110931.
HOGENOMiHOG000007538.
InParanoidiQ96JN8.
KOiK16777.
OMAiGKCTQVT.
OrthoDBiEOG7XWPMQ.
PhylomeDBiQ96JN8.

Miscellaneous databases

EvolutionaryTraceiQ96JN8.
GenomeRNAii84461.
PROiQ96JN8.
SOURCEiSearch...

Gene expression databases

BgeeiQ96JN8.
ExpressionAtlasiQ96JN8. baseline and differential.
GenevisibleiQ96JN8. HS.

Family and domain databases

InterProiIPR013320. ConA-like_dom.
IPR006573. NHR_dom.
[Graphical view]
PfamiPF07177. Neuralized. 6 hits.
[Graphical view]
SMARTiSM00588. NEUZ. 6 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiPS51065. NHR. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.
    DNA Res. 8:85-95(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 47-1562 (ISOFORM 2).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 735-1562 (ISOFORM 2), VARIANT HIS-1019.
    Tissue: Kidney and Testis.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-907, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Neurl4, a novel daughter centriole protein, prevents formation of ectopic microtubule organizing centres."
    Li J., Kim S., Kobayashi T., Liang F.X., Korzeniewski N., Duensing S., Dynlacht B.D.
    EMBO Rep. 13:547-553(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CCP110, SUBCELLULAR LOCATION.
  7. "Identification and proteomic analysis of distinct UBE3A/E6AP protein complexes."
    Martinez-Noel G., Galligan J.T., Sowa M.E., Arndt V., Overton T.M., Harper J.W., Howley P.M.
    Mol. Cell. Biol. 32:3095-3106(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UBE3A AND MAPK6.
  8. "Interaction proteomics identify NEURL4 and the HECT E3 ligase HERC2 as novel modulators of centrosome architecture."
    Al-Hakim A.K., Bashkurov M., Gingras A.C., Durocher D., Pelletier L.
    Mol. Cell. Proteomics 11:M111.014233.01-M111.014233.14(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CEP97; CCP110 AND HERC2, SUBCELLULAR LOCATION, UBIQUITINATION.
  9. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-502, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma and Erythroleukemia.
  10. "Solution structure of the NEUZ domain in KIAA1787 protein."
    RIKEN structural genomics initiative (RSGI)
    Submitted (JUN-2007) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 42-205.

Entry informationi

Entry nameiNEUL4_HUMAN
AccessioniPrimary (citable) accession number: Q96JN8
Secondary accession number(s): Q6GPI8, Q96IU9, Q9H0B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: August 21, 2007
Last modified: July 6, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.