Q96JN0 (LCOR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ligand-dependent corepressor
Short name=LCoR

Alternative name(s):
Mblk1-related protein 2

Gene names

Name:	LCOR
Synonyms:	KIAA1795, MLR2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act as transcription activator that binds DNA elements with the sequence 5'-CCCTATCGATCGATCTCTACCT-3' By similarity. Repressor of ligand-dependent transcription activation by target nuclear receptors. Repressor of ligand-dependent transcription activation by ESR1, ESR2, NR3C1, PGR, RARA, RARB, RARG, RXRA and VDR. Ref.6
Subunit structure	Interacts with ESR1 and ESR2 in the presence of estradiol. Interacts with CTBP1, HDAC3 and HDAC6. Component of a large corepressor complex that contains about 20 proteins, including CTBP1, CTBP2, HDAC1 and HDAC2. Ref.6 Ref.7
Subcellular location	Nucleus Ref.6.
Tissue specificity	Ubiquitous. Ref.6
Sequence similarities	Contains 1 HTH psq-type DNA-binding domain.
Sequence caution	The sequence BAB47424.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator Repressor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96JN0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96JN0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     405-406: RS → SG
     407-433: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 433

433

Ligand-dependent corepressor

PRO_0000236807

Regions

Domain

340 – 392

53

HTH psq-type

DNA binding

368 – 388

21

H-T-H motif By similarity

Motif

53 – 57

5

Interaction with nuclear receptors

Motif

339 – 345

7

Nuclear localization signal Potential

Amino acid modifications

Modified residue

63

1

Phosphoserine Ref.9

Modified residue

249

1

Phosphoserine Ref.9

Natural variations

Alternative sequence

405 – 406

2

RS → SG in isoform 2.

VSP_018585

Alternative sequence

407 – 433

27

Missing in isoform 2.

VSP_018586

Experimental info

Mutagenesis

56 – 57

2

LL → AA: Loss of estradiol-dependent interaction with ESR1 and ESR2. Ref.6

Sequence conflict

6

1

Q → P in CAD91159. Ref.2

Sequence conflict

321

1

S → P in CAD38921. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

433

Helix Strand Turn

Details...

Helix

351 – 362

12

Helix

368 – 375

8

Helix

379 – 389

11

Turn

390 – 393

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 30, 2006. Version 2. Checksum: 5F934FE687417740	FASTA	433	47,007
	10 20 30 40 50 60 MQRMIQQFAA EYTSKNSSTQ DPSQPNSTKN QSLPKASPVT TSPTAATTQN PVLSKLLMAD 70 80 90 100 110 120 QDSPLDLTVR KSQSEPSEQD GVLDLSTKKS PCAGSTSLSH SPGCSSTQGN GRPGRPSQYR 130 140 150 160 170 180 PDGLRSGDGV PPRSLQDGTR EGFGHSTSLK VPLARSLQIS EELLSRNQLS TAASLGPSGL 190 200 210 220 230 240 QNHGQHLILS REASWAKPHY EFNLSRMKFR GNGALSNISD LPFLAENSAF PKMALQAKQD 250 260 270 280 290 300 GKKDVSHSSP VDLKIPQVRG MDLSWESRTG DQYSYSSLVM GSQTESALSK KLRAILPKQS 310 320 330 340 350 360 RKSMLDAGPD SWGSDAEQST SGQPYPTSDQ EGDPGSKQPR KKRGRYRQYN SEILEEAISV 370 380 390 400 410 420 VMSGKMSVSK AQSIYGIPHS TLEYKVKERL GTLKNPPKKK MKLMRSEGPD VSVKIELDPQ 430 GEAAQSANES KNE
	Isoform 2 [UniParc]. Checksum: ACE760CF3CB1F2EF	FASTA	406	44,083
	10 20 30 40 50 60 MQRMIQQFAA EYTSKNSSTQ DPSQPNSTKN QSLPKASPVT TSPTAATTQN PVLSKLLMAD 70 80 90 100 110 120 QDSPLDLTVR KSQSEPSEQD GVLDLSTKKS PCAGSTSLSH SPGCSSTQGN GRPGRPSQYR 130 140 150 160 170 180 PDGLRSGDGV PPRSLQDGTR EGFGHSTSLK VPLARSLQIS EELLSRNQLS TAASLGPSGL 190 200 210 220 230 240 QNHGQHLILS REASWAKPHY EFNLSRMKFR GNGALSNISD LPFLAENSAF PKMALQAKQD 250 260 270 280 290 300 GKKDVSHSSP VDLKIPQVRG MDLSWESRTG DQYSYSSLVM GSQTESALSK KLRAILPKQS 310 320 330 340 350 360 RKSMLDAGPD SWGSDAEQST SGQPYPTSDQ EGDPGSKQPR KKRGRYRQYN SEILEEAISV 370 380 390 400 VMSGKMSVSK AQSIYGIPHS TLEYKVKERL GTLKNPPKKK MKLMSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O. DNA Res. 8:85-95(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Bone marrow and Skeletal muscle.
[3]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung.
[6]	"Ligand-dependent nuclear receptor corepressor LCoR functions by histone deacetylase-dependent and -independent mechanisms." Fernandes I., Bastien Y., Wai T., Nygard K., Lin R., Cormier O., Lee H.S., Eng F., Bertos N.R., Pelletier N., Mader S., Han V.K.M., Yang X.-J., White J.H. Mol. Cell 11:139-150(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CTBP1; ESR1; ESR2; HDAC3 AND HDAC6, SUBCELLULAR LOCATION, MUTAGENESIS OF 56-LEU-LEU-57, TISSUE SPECIFICITY.
[7]	"Coordinated histone modifications mediated by a CtBP co-repressor complex." Shi Y., Sawada J., Sui G., Affar E.B., Whetstine J.R., Lan F., Ogawa H., Luke M.P.-S., Nakatani Y., Shi Y. Nature 422:735-738(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COREPRESSOR COMPLEX, MASS SPECTROMETRY.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63 AND SER-249, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Solution structures of the HTH domain of human LCOR protein." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 343-405.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB058698 mRNA. Translation: BAB47424.1. Different initiation.
AL834245 mRNA. Translation: CAD38921.2.
AL832106 mRNA. Translation: CAD91159.1.
AL832044 mRNA. Translation: CAD91160.1.
AL442123 Genomic DNA. Translation: CAH70915.1.
CH471066 Genomic DNA. Translation: EAW49963.1.
CH471066 Genomic DNA. Translation: EAW49965.1.
CH471066 Genomic DNA. Translation: EAW49966.1.
BC053359 mRNA. Translation: AAH53359.1.

IPI

IPI00478371.
IPI00759763.

RefSeq

NP_001164236.1. NM_001170765.1.
NP_001164237.1. NM_001170766.1.
NP_115816.2. NM_032440.3.

UniGene

Hs.644719.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2COB	NMR	-	A	343-405	[»]

ProteinModelPortal

Q96JN0.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000348298.

PTM databases

PhosphoSite

Q96JN0.

Polymorphism databases

DMDM

108936028.

Proteomic databases

PaxDb

Q96JN0.

PRIDE

Q96JN0.

Protocols and materials databases

DNASU

84458.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000356016; ENSP00000348298; ENSG00000196233.
ENST00000371097; ENSP00000360138; ENSG00000196233.
ENST00000371103; ENSP00000360144; ENSG00000196233.
ENST00000540664; ENSP00000443431; ENSG00000196233.

GeneID

84458.

KEGG

hsa:84458.

UCSC

uc001kmr.3. human.
uc001kms.2. human.

Organism-specific databases

CTD

84458.

GeneCards

GC10P098583.

HGNC

HGNC:29503. LCOR.

HPA

HPA031428.

MIM

607698. gene.

neXtProt

NX_Q96JN0.

PharmGKB

PA145148487.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG309364.

HOVERGEN

HBG079596.

InParanoid

Q96JN0.

OMA

IMQRMIQ.

OrthoDB

EOG4XWFZ4.

Gene expression databases

Bgee

Q96JN0.

CleanEx

HS_LCOR.

Genevestigator

Q96JN0.

GermOnline

ENSG00000196233. Homo sapiens.

Family and domain databases

InterPro

IPR009057. Homeodomain-like.
IPR007889. HTH_Psq.
[Graphical view]

Pfam

PF05225. HTH_psq. 1 hit.
[Graphical view]

SUPFAM

SSF46689. Homeodomain_like. 1 hit.

PROSITE

PS50960. HTH_PSQ. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q96JN0.

GenomeRNAi

84458.

NextBio

74251.

SOURCE

Search...

Entry information

Entry name

LCOR_HUMAN

Accession

Primary (citable) accession number: Q96JN0
Secondary accession number(s): D3DR47 , Q5VW16, Q7Z723, Q86T32, Q86T33, Q8N3L6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	May 30, 2006
Last modified:	May 29, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.