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Protein

Chromosome alignment-maintaining phosphoprotein 1

Gene

CHAMP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri738 – 760C2H2-typePROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

  • attachment of mitotic spindle microtubules to kinetochore Source: UniProtKB
  • protein localization to kinetochore Source: UniProtKB
  • protein localization to microtubule Source: UniProtKB
  • sister chromatid biorientation Source: UniProtKB

Keywordsi

LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome alignment-maintaining phosphoprotein 1
Alternative name(s):
Zinc finger protein 828
Gene namesi
Name:CHAMP1
Synonyms:C13orf8, CAMP, CHAMP, KIAA1802, ZNF828
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000198824.5.
HGNCiHGNC:20311. CHAMP1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

Pathology & Biotechi

Involvement in diseasei

Mental retardation, autosomal dominant 40 (MRD40)2 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period.
See also OMIM:616579

Keywords - Diseasei

Mental retardation

Organism-specific databases

DisGeNETi283489.
MalaCardsiCHAMP1.
MIMi616579. phenotype.
OpenTargetsiENSG00000198824.
PharmGKBiPA162410749.

Polymorphism and mutation databases

BioMutaiCHAMP1.
DMDMi114149935.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002483191 – 812Chromosome alignment-maintaining phosphoprotein 1Add BLAST812

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei87PhosphoserineCombined sources1
Modified residuei108PhosphoserineCombined sources1
Modified residuei173PhosphoserineCombined sources1
Modified residuei184PhosphoserineCombined sources1
Modified residuei204PhosphoserineCombined sources1
Modified residuei214PhosphoserineCombined sources1
Modified residuei217PhosphoserineCombined sources1
Modified residuei244PhosphoserineBy similarity1
Modified residuei247PhosphoserineCombined sources1
Modified residuei253PhosphoserineCombined sources1
Modified residuei264PhosphoserineCombined sources1
Modified residuei275PhosphoserineCombined sources1
Modified residuei282PhosphoserineCombined sources1
Modified residuei286PhosphoserineCombined sources1
Modified residuei297PhosphoserineCombined sources1
Modified residuei308PhosphoserineCombined sources1
Modified residuei319PhosphoserineCombined sources1
Modified residuei344PhosphoserineCombined sources1
Modified residuei355PhosphoserineCombined sources1
Modified residuei376PhosphoserineCombined sources1
Modified residuei382PhosphoserineCombined sources1
Modified residuei386PhosphoserineCombined sources1
Modified residuei403PhosphothreonineCombined sources1
Modified residuei405PhosphoserineCombined sources1
Modified residuei416PhosphoserineCombined sources1
Modified residuei427PhosphoserineCombined sources1
Modified residuei432PhosphoserineCombined sources1
Modified residuei436PhosphoserineCombined sources1
Modified residuei443PhosphoserineCombined sources1
Modified residuei445PhosphoserineCombined sources1
Modified residuei452PhosphoserineCombined sources1
Modified residuei458PhosphothreonineCombined sources1
Modified residuei459PhosphoserineCombined sources1
Modified residuei462PhosphoserineCombined sources1
Modified residuei472PhosphoserineCombined sources1
Modified residuei476PhosphoserineCombined sources1
Modified residuei490N6-acetyllysine; alternateCombined sources1
Cross-linki490Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei507PhosphoserineCombined sources1
Modified residuei512PhosphoserineCombined sources1
Modified residuei542PhosphoserineCombined sources1
Cross-linki565Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei572PhosphoserineCombined sources1
Modified residuei603PhosphoserineCombined sources1
Cross-linki606Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei615PhosphoserineCombined sources1
Modified residuei626PhosphoserineCombined sources1
Modified residuei627PhosphoserineCombined sources1
Modified residuei632PhosphoserineCombined sources1
Cross-linki638Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei651PhosphoserineCombined sources1
Modified residuei652PhosphoserineCombined sources1
Modified residuei653PhosphoserineCombined sources1
Cross-linki670Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei675PhosphoserineCombined sources1
Cross-linki689Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei736PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by CDK1. Mitotic phosphorylation is required for the attachment of spindle microtubules to the kinetochore.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ96JM3.
MaxQBiQ96JM3.
PaxDbiQ96JM3.
PeptideAtlasiQ96JM3.
PRIDEiQ96JM3.

PTM databases

iPTMnetiQ96JM3.
PhosphoSitePlusiQ96JM3.

Expressioni

Gene expression databases

BgeeiENSG00000198824.
CleanExiHS_ZNF828.
ExpressionAtlasiQ96JM3. baseline and differential.
GenevisibleiQ96JM3. HS.

Organism-specific databases

HPAiHPA006623.
HPA008900.

Interactioni

Subunit structurei

Interacts with MAD2L2. Interacts with POGZ, CBX1, CBX3 and CBX5.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MAD2L2Q9UI954EBI-2560420,EBI-77889

Protein-protein interaction databases

BioGridi129584. 43 interactors.
IntActiQ96JM3. 25 interactors.
STRINGi9606.ENSP00000354730.

Structurei

3D structure databases

ProteinModelPortaliQ96JM3.
SMRiQ96JM3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni271 – 490Mediates interaction with MAD2L21 PublicationAdd BLAST220
Regioni451 – 590Mediates localization to the spindle and the kinetochore and is required for the attachment of spindle microtubules to the kinetochoreAdd BLAST140
Regioni591 – 812Mediates localization to the chromosome and the spindle and negatively regulates chromosome alignmentAdd BLAST222

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi105 – 564Pro-richAdd BLAST460

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri738 – 760C2H2-typePROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IFC8. Eukaryota.
ENOG410XXII. LUCA.
GeneTreeiENSGT00730000111351.
HOGENOMiHOG000065731.
HOVERGENiHBG062352.
InParanoidiQ96JM3.
OMAiPEFCDEM.
OrthoDBiEOG091G02UO.
PhylomeDBiQ96JM3.
TreeFamiTF350859.

Family and domain databases

InterProiView protein in InterPro
IPR036236. Znf_C2H2_sf.
IPR013087. Znf_C2H2_type.
SMARTiView protein in SMART
SM00355. ZnF_C2H2. 5 hits.
SUPFAMiSSF57667. SSF57667. 1 hit.
PROSITEiView protein in PROSITE
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q96JM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAFQELRKP SARLECDHCS FRGTDYENVQ IHMGTIHPEF CDEMDAGGLG
60 70 80 90 100
KMIFYQKSAK LFHCHKCFFT SKMYSNVYYH ITSKHASPDK WNDKPKNQLN
110 120 130 140 150
KETDPVKSPP LPEHQKIPCN SAEPKSIPAL SMETQKLGSV LSPESPKPTP
160 170 180 190 200
LTPLEPQKPG SVVSPELQTP LPSPEPSKPA SVSSPEPPKS VPVCESQKLA
210 220 230 240 250
PVPSPEPQKP APVSPESVKA TLSNPKPQKQ SHFPETLGPP SASSPESPVL
260 270 280 290 300
AASPEPWGPS PAASPESRKS ARTTSPEPRK PSPSESPEPW KPFPAVSPEP
310 320 330 340 350
RRPAPAVSPG SWKPGPPGSP RPWKSNPSAS SGPWKPAKPA PSVSPGPWKP
360 370 380 390 400
IPSVSPGPWK PTPSVSSASW KSSSVSPSSW KSPPASPESW KSGPPELRKT
410 420 430 440 450
APTLSPEHWK AVPPVSPELR KPGPPLSPEI RSPAGSPELR KPSGSPDLWK
460 470 480 490 500
LSPDQRKTSP ASLDFPESQK SSRGGSPDLW KSSFFIEPQK PVFPETRKPG
510 520 530 540 550
PSGPSESPKA ASDIWKPVLS IDTEPRKPAL FPEPAKTAPP ASPEARKRAL
560 570 580 590 600
FPEPRKHALF PELPKSALFS ESQKAVELGD ELQIDAIDDQ KCDILVQEEL
610 620 630 640 650
LASPKKLLED TLFPSSKKLK KDNQESSDAE LSSSEYIKTD LDAMDIKGQE
660 670 680 690 700
SSSDQEQVDV ESIDFSKENK MDMTSPEQSR NVLQFTEEKE AFISEEEIAK
710 720 730 740 750
YMKRGKGKYY CKICCCRAMK KGAVLHHLVN KHNVHSPYKC TICGKAFLLE
760 770 780 790 800
SLLKNHVAAH GQSLLKCPRC NFESNFPRGF KKHLTHCQSR HNEEANKKLM
810
EALEPPLEEQ QI
Length:812
Mass (Da):89,099
Last modified:September 5, 2006 - v2
Checksum:i0276EF84795D89F2
GO

Sequence cautioni

Q96JM3: The sequence BAB47431 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
Q96JM3: The sequence BAC11273 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti81I → T in BAC11273 (PubMed:14702039).Curated1
Sequence conflicti140V → A in BAC11273 (PubMed:14702039).Curated1
Sequence conflicti576V → A in BAC11273 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027270568L → V. Corresponds to variant dbSNP:rs3764522Ensembl.1
Natural variantiVAR_052910591K → R. Corresponds to variant dbSNP:rs35564629Ensembl.1
Natural variantiVAR_027271604P → R. Corresponds to variant dbSNP:rs12428067Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058705 mRNA. Translation: BAB47431.1. Different initiation.
AK074894 mRNA. Translation: BAC11273.1. Different initiation.
AK096346 mRNA. Translation: BAG53268.1.
AL845154 Genomic DNA. No translation available.
CH471085 Genomic DNA. Translation: EAX09253.1.
BC004820 mRNA. Translation: AAH04820.1.
BC065237 mRNA. Translation: AAH65237.1.
CCDSiCCDS9545.1.
RefSeqiNP_001157616.1. NM_001164144.2.
NP_001157617.1. NM_001164145.2.
NP_115812.1. NM_032436.3.
UniGeneiHs.7542.

Genome annotation databases

EnsembliENST00000361283; ENSP00000354730; ENSG00000198824.
GeneIDi283489.
KEGGihsa:283489.
UCSCiuc001vuv.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCHAP1_HUMAN
AccessioniPrimary (citable) accession number: Q96JM3
Secondary accession number(s): B3KU06
, Q6P181, Q8NC88, Q9BST0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: October 25, 2017
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot