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Protein

DDB1- and CUL4-associated factor 5

Gene

DCAF5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

SignaLinkiQ96JK2.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
DDB1- and CUL4-associated factor 5
Alternative name(s):
Breakpoint cluster region protein 2
Short name:
BCRP2
WD repeat-containing protein 22
Gene namesi
Name:DCAF5
Synonyms:BCRG2, KIAA1824, WDR22
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:20224. DCAF5.

Subcellular locationi

GO - Cellular componenti

  • Cul4-RING E3 ubiquitin ligase complex Source: UniProtKB
  • mitochondrion Source: Ensembl
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165478844.

Polymorphism and mutation databases

BioMutaiDCAF5.
DMDMi47606200.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 942942DDB1- and CUL4-associated factor 5PRO_0000051369Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei500 – 5001PhosphothreonineBy similarity
Modified residuei531 – 5311PhosphoserineBy similarity
Modified residuei533 – 5331PhosphoserineBy similarity
Modified residuei626 – 6261PhosphoserineBy similarity
Modified residuei628 – 6281PhosphoserineBy similarity
Modified residuei645 – 6451PhosphoserineCombined sources
Modified residuei648 – 6481PhosphoserineCombined sources
Modified residuei651 – 6511PhosphoserineCombined sources
Modified residuei794 – 7941PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ96JK2.
MaxQBiQ96JK2.
PaxDbiQ96JK2.
PRIDEiQ96JK2.

PTM databases

iPTMnetiQ96JK2.
PhosphoSiteiQ96JK2.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiQ96JK2.
CleanExiHS_WDR22.
ExpressionAtlasiQ96JK2. baseline and differential.
GenevisibleiQ96JK2. HS.

Organism-specific databases

HPAiHPA041575.
HPA048098.

Interactioni

Subunit structurei

Interacts with DDB1 and CUL4A.1 Publication

Protein-protein interaction databases

BioGridi114343. 38 interactions.
DIPiDIP-48763N.
IntActiQ96JK2. 1 interaction.
STRINGi9606.ENSP00000341351.

Structurei

Secondary structure

1
942
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 185Combined sources
Turni19 – 235Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3I89X-ray3.00B13-25[»]
ProteinModelPortaliQ96JK2.
SMRiQ96JK2. Positions 14-363.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96JK2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati51 – 9141WD 1Add
BLAST
Repeati99 – 13941WD 2Add
BLAST
Repeati140 – 18041WD 3Add
BLAST
Repeati185 – 22541WD 4Add
BLAST
Repeati277 – 31741WD 5Add
BLAST
Repeati331 – 37040WD 6Add
BLAST

Sequence similaritiesi

Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG4227. Eukaryota.
ENOG410ZPFW. LUCA.
GeneTreeiENSGT00530000062951.
HOGENOMiHOG000060080.
HOVERGENiHBG057544.
InParanoidiQ96JK2.
KOiK11800.
OMAiCANHNNG.
OrthoDBiEOG7DNNVS.
PhylomeDBiQ96JK2.
TreeFamiTF320710.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96JK2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKRRAGLGGS MRSVVGFLSQ RGLHGDPLLT QDFQRRRLRG CRNLYKKDLL
60 70 80 90 100
GHFGCVNAIE FSNNGGQWLV SGGDDRRVLL WHMEQAIHSR VKPIQLKGEH
110 120 130 140 150
HSNIFCLAFN SGNTKVFSGG NDEQVILHDV ESSETLDVFA HEDAVYGLSV
160 170 180 190 200
SPVNDNIFAS SSDDGRVLIW DIRESPHGEP FCLANYPSAF HSVMFNPVEP
210 220 230 240 250
RLLATANSKE GVGLWDIRKP QSSLLRYGGN LSLQSAMSVR FNSNGTQLLA
260 270 280 290 300
LRRRLPPVLY DIHSRLPVFQ FDNQGYFNSC TMKSCCFAGD RDQYILSGSD
310 320 330 340 350
DFNLYMWRIP ADPEAGGIGR VVNGAFMVLK GHRSIVNQVR FNPHTYMICS
360 370 380 390 400
SGVEKIIKIW SPYKQPGCTG DLDGRIEDDS RCLYTHEEYI SLVLNSGSGL
410 420 430 440 450
SHDYANQSVQ EDPRMMAFFD SLVRREIEGW SSDSDSDLSE STILQLHAGV
460 470 480 490 500
SERSGYTDSE SSASLPRSPP PTVDESADNA FHLGPLRVTT TNTVASTPPT
510 520 530 540 550
PTCEDAASRQ QRLSALRRYQ DKRLLALSNE SDSEENVCEV ELDTDLFPRP
560 570 580 590 600
RSPSPEDESS SSSSSSSSED EEELNERRAS TWQRNAMRRR QKTTREDKPS
610 620 630 640 650
APIKPTNTYI GEDNYDYPQI KVDDLSSSPT SSPERSTSTL EIQPSRASPT
660 670 680 690 700
SDIESVERKI YKAYKWLRYS YISYSNNKDG ETSLVTGEAD EGRAGTSHKD
710 720 730 740 750
NPAPSSSKEA CLNIAMAQRN QDLPPEGCSK DTFKEETPRT PSNGPGHEHS
760 770 780 790 800
SHAWAEVPEG TSQDTGNSGS VEHPFETKKL NGKALSSRAE EPPSPPVPKA
810 820 830 840 850
SGSTLNSGSG NCPRTQSDDS EERSLETICA NHNNGRLHPR PPHPHNNGQN
860 870 880 890 900
LGELEVVAYS SPGHSDTDRD NSSLTGTLLH KDCCGSEMAC ETPNAGTRED
910 920 930 940
PTDTPATDSS RAVHGHSGLK RQRIELEDTD SENSSSEKKL KT
Length:942
Mass (Da):103,963
Last modified:May 24, 2004 - v2
Checksum:i3C627BA3FB2DC522
GO
Isoform 2 (identifier: Q96JK2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Note: No experimental confirmation available.
Show »
Length:860
Mass (Da):94,699
Checksum:i5580E97E0C3ACA58
GO
Isoform 3 (identifier: Q96JK2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     132-132: Missing.

Note: No experimental confirmation available. Gene prediction based on cDNA data.
Show »
Length:941
Mass (Da):103,876
Checksum:i05A2AF1D551E965B
GO

Sequence cautioni

The sequence AAC08965.1 differs from that shown. Reason: Frameshift at positions 772, 809, 812, 817, 836, 844 and 858. Curated
The sequence BAB47453.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti149 – 1491S → F (PubMed:17974005).Curated
Sequence conflicti404 – 4041Y → H in AAC08965 (PubMed:9740667).Curated
Sequence conflicti607 – 6071N → D in AAC08965 (PubMed:9740667).Curated
Sequence conflicti836 – 8416RLHPRP → TLHLS in AAC08965 (PubMed:9740667).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8282Missing in isoform 2. 1 PublicationVSP_010386Add
BLAST
Alternative sequencei132 – 1321Missing in isoform 3. CuratedVSP_055647

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058727 mRNA. Translation: BAB47453.1. Different initiation.
AL391262 Genomic DNA. No translation available.
AL831932 mRNA. Translation: CAD38589.1.
AL831823 mRNA. Translation: CAD38537.2.
CH471061 Genomic DNA. Translation: EAW80981.1.
CH471061 Genomic DNA. Translation: EAW80983.1.
BC136632 mRNA. Translation: AAI36633.1.
AF044774 mRNA. Translation: AAC08965.1. Frameshift.
CCDSiCCDS32106.1. [Q96JK2-1]
CCDS61480.1. [Q96JK2-2]
CCDS61481.1. [Q96JK2-3]
RefSeqiNP_001271135.1. NM_001284206.1. [Q96JK2-3]
NP_001271136.1. NM_001284207.1. [Q96JK2-2]
NP_003852.1. NM_003861.2. [Q96JK2-1]
UniGeneiHs.509780.

Genome annotation databases

EnsembliENST00000341516; ENSP00000341351; ENSG00000139990. [Q96JK2-1]
ENST00000554215; ENSP00000451551; ENSG00000139990. [Q96JK2-2]
ENST00000556847; ENSP00000452052; ENSG00000139990. [Q96JK2-2]
ENST00000557386; ENSP00000451845; ENSG00000139990. [Q96JK2-3]
GeneIDi8816.
KEGGihsa:8816.
UCSCiuc001xkp.4. human. [Q96JK2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058727 mRNA. Translation: BAB47453.1. Different initiation.
AL391262 Genomic DNA. No translation available.
AL831932 mRNA. Translation: CAD38589.1.
AL831823 mRNA. Translation: CAD38537.2.
CH471061 Genomic DNA. Translation: EAW80981.1.
CH471061 Genomic DNA. Translation: EAW80983.1.
BC136632 mRNA. Translation: AAI36633.1.
AF044774 mRNA. Translation: AAC08965.1. Frameshift.
CCDSiCCDS32106.1. [Q96JK2-1]
CCDS61480.1. [Q96JK2-2]
CCDS61481.1. [Q96JK2-3]
RefSeqiNP_001271135.1. NM_001284206.1. [Q96JK2-3]
NP_001271136.1. NM_001284207.1. [Q96JK2-2]
NP_003852.1. NM_003861.2. [Q96JK2-1]
UniGeneiHs.509780.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3I89X-ray3.00B13-25[»]
ProteinModelPortaliQ96JK2.
SMRiQ96JK2. Positions 14-363.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114343. 38 interactions.
DIPiDIP-48763N.
IntActiQ96JK2. 1 interaction.
STRINGi9606.ENSP00000341351.

PTM databases

iPTMnetiQ96JK2.
PhosphoSiteiQ96JK2.

Polymorphism and mutation databases

BioMutaiDCAF5.
DMDMi47606200.

Proteomic databases

EPDiQ96JK2.
MaxQBiQ96JK2.
PaxDbiQ96JK2.
PRIDEiQ96JK2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000341516; ENSP00000341351; ENSG00000139990. [Q96JK2-1]
ENST00000554215; ENSP00000451551; ENSG00000139990. [Q96JK2-2]
ENST00000556847; ENSP00000452052; ENSG00000139990. [Q96JK2-2]
ENST00000557386; ENSP00000451845; ENSG00000139990. [Q96JK2-3]
GeneIDi8816.
KEGGihsa:8816.
UCSCiuc001xkp.4. human. [Q96JK2-1]

Organism-specific databases

CTDi8816.
GeneCardsiDCAF5.
HGNCiHGNC:20224. DCAF5.
HPAiHPA041575.
HPA048098.
MIMi603812. gene.
neXtProtiNX_Q96JK2.
PharmGKBiPA165478844.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4227. Eukaryota.
ENOG410ZPFW. LUCA.
GeneTreeiENSGT00530000062951.
HOGENOMiHOG000060080.
HOVERGENiHBG057544.
InParanoidiQ96JK2.
KOiK11800.
OMAiCANHNNG.
OrthoDBiEOG7DNNVS.
PhylomeDBiQ96JK2.
TreeFamiTF320710.

Enzyme and pathway databases

UniPathwayiUPA00143.
SignaLinkiQ96JK2.

Miscellaneous databases

ChiTaRSiDCAF5. human.
EvolutionaryTraceiQ96JK2.
GenomeRNAii8816.
PROiQ96JK2.
SOURCEiSearch...

Gene expression databases

BgeeiQ96JK2.
CleanExiHS_WDR22.
ExpressionAtlasiQ96JK2. baseline and differential.
GenevisibleiQ96JK2. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.
    DNA Res. 8:85-95(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Skeletal muscle.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Genomic and functional map of the chromosome 14 t(12;14) breakpoint cluster region in uterine leiomyoma."
    Lynch R.A., Piper M., Bankier A., Bhugra B., Surti U., Liu J., Buckler A., Dear P.H., Menon A.G.
    Genomics 52:17-26(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 262-942, TISSUE SPECIFICITY.
    Tissue: Uterine leiomyoma.
  6. "A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1."
    Jin J., Arias E.E., Chen J., Harper J.W., Walter J.C.
    Mol. Cell 23:709-721(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH DDB1 AND CUL4A, IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery."
    Angers S., Li T., Yi X., MacCoss M.J., Moon R.T., Zheng N.
    Nature 443:590-593(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-648 AND SER-651, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-645; SER-648 AND SER-794, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiDCAF5_HUMAN
AccessioniPrimary (citable) accession number: Q96JK2
Secondary accession number(s): B2RN31
, G3V4J7, O60559, Q8N3V3, Q8N3V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: June 8, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.