Q96JK2 (DCAF5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DDB1- and CUL4-associated factor 5

Alternative name(s):
Breakpoint cluster region protein 2
Short name=BCRP2
WD repeat-containing protein 22

Gene names

Name:	DCAF5
Synonyms:	BCRG2, KIAA1824, WDR22

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	942 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. Ref.6
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with DDB1 and CUL4A. Ref.6
Tissue specificity	Ubiquitous. Ref.5
Sequence similarities	Contains 6 WD repeats.
Sequence caution	The sequence AAC08965.1 differs from that shown. Reason: Frameshift at positions 772, 809, 812, 817, 836, 844 and 858. The sequence BAB47453.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein ubiquitination Inferred by curator Ref.6. Source: UniProtKB
Cellular component	CUL4 RING ubiquitin ligase complex Inferred from direct assay Ref.6. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96JK2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96JK2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 942

942

DDB1- and CUL4-associated factor 5

PRO_0000051369

Regions

Repeat

51 – 91

41

WD 1

Repeat

99 – 139

41

WD 2

Repeat

140 – 180

41

WD 3

Repeat

185 – 225

41

WD 4

Repeat

277 – 317

41

WD 5

Repeat

331 – 370

40

WD 6

Amino acid modifications

Modified residue

626

1

Phosphoserine By similarity

Modified residue

628

1

Phosphoserine By similarity

Modified residue

631

1

Phosphoserine By similarity

Modified residue

632

1

Phosphoserine By similarity

Modified residue

648

1

Phosphoserine Ref.7

Modified residue

651

1

Phosphoserine Ref.7

Natural variations

Alternative sequence

1 – 82

82

Missing in isoform 2.

VSP_010386

Experimental info

Sequence conflict

149

1

S → F Ref.2

Sequence conflict

404

1

Y → H in AAC08965. Ref.5

Sequence conflict

607

1

N → D in AAC08965. Ref.5

Sequence conflict

836 – 841

6

RLHPRP → TLHLS in AAC08965. Ref.5

Secondary structure

1

.

.

.

942

Helix Strand Turn

Details...

Turn

18 – 20

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 24, 2004. Version 2. Checksum: 3C627BA3FB2DC522	FASTA	942	103,963
	10 20 30 40 50 60 MKRRAGLGGS MRSVVGFLSQ RGLHGDPLLT QDFQRRRLRG CRNLYKKDLL GHFGCVNAIE 70 80 90 100 110 120 FSNNGGQWLV SGGDDRRVLL WHMEQAIHSR VKPIQLKGEH HSNIFCLAFN SGNTKVFSGG 130 140 150 160 170 180 NDEQVILHDV ESSETLDVFA HEDAVYGLSV SPVNDNIFAS SSDDGRVLIW DIRESPHGEP 190 200 210 220 230 240 FCLANYPSAF HSVMFNPVEP RLLATANSKE GVGLWDIRKP QSSLLRYGGN LSLQSAMSVR 250 260 270 280 290 300 FNSNGTQLLA LRRRLPPVLY DIHSRLPVFQ FDNQGYFNSC TMKSCCFAGD RDQYILSGSD 310 320 330 340 350 360 DFNLYMWRIP ADPEAGGIGR VVNGAFMVLK GHRSIVNQVR FNPHTYMICS SGVEKIIKIW 370 380 390 400 410 420 SPYKQPGCTG DLDGRIEDDS RCLYTHEEYI SLVLNSGSGL SHDYANQSVQ EDPRMMAFFD 430 440 450 460 470 480 SLVRREIEGW SSDSDSDLSE STILQLHAGV SERSGYTDSE SSASLPRSPP PTVDESADNA 490 500 510 520 530 540 FHLGPLRVTT TNTVASTPPT PTCEDAASRQ QRLSALRRYQ DKRLLALSNE SDSEENVCEV 550 560 570 580 590 600 ELDTDLFPRP RSPSPEDESS SSSSSSSSED EEELNERRAS TWQRNAMRRR QKTTREDKPS 610 620 630 640 650 660 APIKPTNTYI GEDNYDYPQI KVDDLSSSPT SSPERSTSTL EIQPSRASPT SDIESVERKI 670 680 690 700 710 720 YKAYKWLRYS YISYSNNKDG ETSLVTGEAD EGRAGTSHKD NPAPSSSKEA CLNIAMAQRN 730 740 750 760 770 780 QDLPPEGCSK DTFKEETPRT PSNGPGHEHS SHAWAEVPEG TSQDTGNSGS VEHPFETKKL 790 800 810 820 830 840 NGKALSSRAE EPPSPPVPKA SGSTLNSGSG NCPRTQSDDS EERSLETICA NHNNGRLHPR 850 860 870 880 890 900 PPHPHNNGQN LGELEVVAYS SPGHSDTDRD NSSLTGTLLH KDCCGSEMAC ETPNAGTRED 910 920 930 940 PTDTPATDSS RAVHGHSGLK RQRIELEDTD SENSSSEKKL KT
	Isoform 2 [UniParc]. Checksum: 5580E97E0C3ACA58	FASTA	860	94,699
	10 20 30 40 50 60 MEQAIHSRVK PIQLKGEHHS NIFCLAFNSG NTKVFSGGND EQVILHDVES SETLDVFAHE 70 80 90 100 110 120 DAVYGLSVSP VNDNIFASSS DDGRVLIWDI RESPHGEPFC LANYPSAFHS VMFNPVEPRL 130 140 150 160 170 180 LATANSKEGV GLWDIRKPQS SLLRYGGNLS LQSAMSVRFN SNGTQLLALR RRLPPVLYDI 190 200 210 220 230 240 HSRLPVFQFD NQGYFNSCTM KSCCFAGDRD QYILSGSDDF NLYMWRIPAD PEAGGIGRVV 250 260 270 280 290 300 NGAFMVLKGH RSIVNQVRFN PHTYMICSSG VEKIIKIWSP YKQPGCTGDL DGRIEDDSRC 310 320 330 340 350 360 LYTHEEYISL VLNSGSGLSH DYANQSVQED PRMMAFFDSL VRREIEGWSS DSDSDLSEST 370 380 390 400 410 420 ILQLHAGVSE RSGYTDSESS ASLPRSPPPT VDESADNAFH LGPLRVTTTN TVASTPPTPT 430 440 450 460 470 480 CEDAASRQQR LSALRRYQDK RLLALSNESD SEENVCEVEL DTDLFPRPRS PSPEDESSSS 490 500 510 520 530 540 SSSSSSEDEE ELNERRASTW QRNAMRRRQK TTREDKPSAP IKPTNTYIGE DNYDYPQIKV 550 560 570 580 590 600 DDLSSSPTSS PERSTSTLEI QPSRASPTSD IESVERKIYK AYKWLRYSYI SYSNNKDGET 610 620 630 640 650 660 SLVTGEADEG RAGTSHKDNP APSSSKEACL NIAMAQRNQD LPPEGCSKDT FKEETPRTPS 670 680 690 700 710 720 NGPGHEHSSH AWAEVPEGTS QDTGNSGSVE HPFETKKLNG KALSSRAEEP PSPPVPKASG 730 740 750 760 770 780 STLNSGSGNC PRTQSDDSEE RSLETICANH NNGRLHPRPP HPHNNGQNLG ELEVVAYSSP 790 800 810 820 830 840 GHSDTDRDNS SLTGTLLHKD CCGSEMACET PNAGTREDPT DTPATDSSRA VHGHSGLKRQ 850 860 RIELEDTDSE NSSSEKKLKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O. DNA Res. 8:85-95(2001) [PubMed: 11347906] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Skeletal muscle.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Genomic and functional map of the chromosome 14 t(12;14) breakpoint cluster region in uterine leiomyoma." Lynch R.A., Piper M., Bankier A., Bhugra B., Surti U., Liu J., Buckler A., Dear P.H., Menon A.G. Genomics 52:17-26(1998) [PubMed: 9740667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 262-942, TISSUE SPECIFICITY. Tissue: Uterine leiomyoma.
[6]	"A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1." Jin J., Arias E.E., Chen J., Harper J.W., Walter J.C. Mol. Cell 23:709-721(2006) [PubMed: 16949367] [Abstract] Cited for: FUNCTION, INTERACTION WITH DDB1 AND CUL4A, IDENTIFICATION BY MASS SPECTROMETRY.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-648 AND SER-651, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB058727 mRNA. Translation: BAB47453.1. Different initiation.
AL831932 mRNA. Translation: CAD38589.1.
AL831823 mRNA. Translation: CAD38537.2.
CH471061 Genomic DNA. Translation: EAW80983.1.
BC136632 mRNA. Translation: AAI36633.1.
AF044774 mRNA. Translation: AAC08965.1. Frameshift.

IPI

IPI00382979.
IPI00783277.

RefSeq

NP_003852.1. NM_003861.2.

UniGene

Hs.509780.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3I89	X-ray	3.00	B	13-25	[»]

ProteinModelPortal

Q96JK2.

SMR

Q96JK2. Positions 47-364.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48763N.

IntAct

Q96JK2. 1 interaction.

STRING

Q96JK2.

PTM databases

PhosphoSite

Q96JK2.

Polymorphism databases

DMDM

47606200.

Proteomic databases

PRIDE

Q96JK2.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000341516; ENSP00000341351; ENSG00000139990.

GeneID

8816.

KEGG

hsa:8816.

UCSC

uc001xkp.1. human.

Organism-specific databases

CTD

8816.

GeneCards

GC14M069517.

H-InvDB

HIX0011762.

HGNC

HGNC:20224. DCAF5.

MIM

603812. gene.

neXtProt

NX_Q96JK2.

PharmGKB

PA134927291.
PA165478844.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG10225.

GeneTree

ENSGT00530000062951.

HOGENOM

HBG717706.

HOVERGEN

HBG057544.

InParanoid

Q96JK2.

OMA

ERKIYKA.

OrthoDB

EOG44MXRG.

PhylomeDB

Q96JK2.

Gene expression databases

ArrayExpress

Q96JK2.

Bgee

Q96JK2.

CleanEx

HS_WDR22.

Genevestigator

Q96JK2.

GermOnline

ENSG00000139990. Homo sapiens.

Family and domain databases

InterPro

IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR011046. WD40_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]

Gene3D

G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.

KO

K11800.

Pfam

PF00400. WD40. 5 hits.
[Graphical view]

SMART

SM00320. WD40. 6 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

33070.

SOURCE

Search...

Entry information

Entry name

DCAF5_HUMAN

Accession

Primary (citable) accession number: Q96JK2
Secondary accession number(s): B2RN31 , O60559, Q8N3V3, Q8N3V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 24, 2004
Last modified:	January 25, 2012
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.