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Protein

Protein disulfide-isomerase TMX3

Gene

TMX3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable disulfide isomerase, which participates in the folding of proteins containing disulfide bonds. May act as a dithiol oxidase.1 Publication

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.1 Publication

Redox potential

E0 is -157 mV.1 Publication

Manual assertion based on experiment ini

GO - Molecular functioni

  • protein disulfide isomerase activity Source: GO_Central
  • thiol oxidase activity Source: WormBase

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciZFISH:HS09404-MONOMER.
ReactomeiR-HSA-114608. Platelet degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase TMX3 (EC:5.3.4.1)
Alternative name(s):
Thioredoxin domain-containing protein 10
Thioredoxin-related transmembrane protein 3
Gene namesi
Name:TMX3
Synonyms:KIAA1830, TXNDC10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:24718. TMX3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 375LumenalSequence analysisAdd BLAST351
Transmembranei376 – 396HelicalSequence analysisAdd BLAST21
Topological domaini397 – 454CytoplasmicSequence analysisAdd BLAST58

GO - Cellular componenti

  • cell surface Source: MGI
  • endoplasmic reticulum membrane Source: WormBase
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: Reactome
  • platelet alpha granule membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi54495.
OpenTargetsiENSG00000166479.
PharmGKBiPA164726632.

Polymorphism and mutation databases

BioMutaiTMX3.
DMDMi78103208.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000003418525 – 454Protein disulfide-isomerase TMX3Add BLAST430

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi53 ↔ 56Redox-activePROSITE-ProRule annotation
Glycosylationi258N-linked (GlcNAc...)1 Publication1
Glycosylationi313N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ96JJ7.
MaxQBiQ96JJ7.
PaxDbiQ96JJ7.
PeptideAtlasiQ96JJ7.
PRIDEiQ96JJ7.
TopDownProteomicsiQ96JJ7-1. [Q96JJ7-1]

PTM databases

iPTMnetiQ96JJ7.
PhosphoSitePlusiQ96JJ7.
SwissPalmiQ96JJ7.

Expressioni

Tissue specificityi

Widely expressed. Expressed in brain, testis, lung, skin, kidney, uterus, bone, stomach, liver, prostate, placenta, eye and muscle.1 Publication

Inductioni

Not up-regulated by unfolded protein response (UPR).1 Publication

Gene expression databases

BgeeiENSG00000166479.
CleanExiHS_TXNDC10.
ExpressionAtlasiQ96JJ7. baseline and differential.
GenevisibleiQ96JJ7. HS.

Organism-specific databases

HPAiHPA014157.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
FHL3Q136433EBI-2514069,EBI-741101

Protein-protein interaction databases

BioGridi119991. 10 interactors.
IntActiQ96JJ7. 20 interactors.
MINTiMINT-3054729.
STRINGi9606.ENSP00000299608.

Structurei

3D structure databases

ProteinModelPortaliQ96JJ7.
SMRiQ96JJ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 128ThioredoxinPROSITE-ProRule annotationAdd BLAST104

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi451 – 454Di-lysine motif4

Domaini

The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.By similarity

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4277. Eukaryota.
COG0526. LUCA.
GeneTreeiENSGT00860000133691.
HOGENOMiHOG000154655.
InParanoidiQ96JJ7.
KOiK09585.
OMAiMQKRHRV.
OrthoDBiEOG091G0MS9.
PhylomeDBiQ96JJ7.
TreeFamiTF313807.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 2 hits.
PROSITEiPS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96JJ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAWKSWTAL RLCATVVVLD MVVCKGFVED LDESFKENRN DDIWLVDFYA
60 70 80 90 100
PWCGHCKKLE PIWNEVGLEM KSIGSPVKVG KMDATSYSSI ASEFGVRGYP
110 120 130 140 150
TIKLLKGDLA YNYRGPRTKD DIIEFAHRVS GALIRPLPSQ QMFEHMQKRH
160 170 180 190 200
RVFFVYVGGE SPLKEKYIDA ASELIVYTYF FSASEEVVPE YVTLKEMPAV
210 220 230 240 250
LVFKDETYFV YDEYEDGDLS SWINRERFQN YLAMDGFLLY ELGDTGKLVA
260 270 280 290 300
LAVIDEKNTS VEHTRLKSII QEVARDYRDL FHRDFQFGHM DGNDYINTLL
310 320 330 340 350
MDELTVPTVV VLNTSNQQYF LLDRQIKNVE DMVQFINNIL DGTVEAQGGD
360 370 380 390 400
SILQRLKRIV FDAKSTIVSI FKSSPLMGCF LFGLPLGVIS IMCYGIYTAD
410 420 430 440 450
TDGGYIEERY EVSKSENENQ EQIEESKEQQ EPSSGGSVVP TVQEPKDVLE

KKKD
Length:454
Mass (Da):51,872
Last modified:May 10, 2005 - v2
Checksum:i37EBA0C70ED84A9B
GO
Isoform 2 (identifier: Q96JJ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     191-195: YVTLK → VIFKI
     196-454: Missing.

Note: No experimental confirmation available.
Show »
Length:195
Mass (Da):22,291
Checksum:i49E7DD4636FF59BE
GO

Sequence cautioni

The sequence BAA91381 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB47459 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02245161P → S.1 PublicationCorresponds to variant rs11557684dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013748191 – 195YVTLK → VIFKI in isoform 2. 1 Publication5
Alternative sequenceiVSP_013749196 – 454Missing in isoform 2. 1 PublicationAdd BLAST259

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058733 mRNA. Translation: BAB47459.1. Different initiation.
AK000800 mRNA. Translation: BAA91381.1. Different initiation.
AK122715 mRNA. Translation: BAG53687.1.
BX647846 mRNA. No translation available.
BC032325 mRNA. Translation: AAH32325.1.
BC093792 mRNA. Translation: AAH93792.1.
BC093794 mRNA. Translation: AAH93794.1.
CCDSiCCDS32840.1. [Q96JJ7-1]
RefSeqiNP_061895.3. NM_019022.3. [Q96JJ7-1]
UniGeneiHs.440534.

Genome annotation databases

EnsembliENST00000299608; ENSP00000299608; ENSG00000166479. [Q96JJ7-1]
ENST00000562706; ENSP00000457262; ENSG00000166479. [Q96JJ7-2]
GeneIDi54495.
KEGGihsa:54495.
UCSCiuc002lkf.4. human. [Q96JJ7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058733 mRNA. Translation: BAB47459.1. Different initiation.
AK000800 mRNA. Translation: BAA91381.1. Different initiation.
AK122715 mRNA. Translation: BAG53687.1.
BX647846 mRNA. No translation available.
BC032325 mRNA. Translation: AAH32325.1.
BC093792 mRNA. Translation: AAH93792.1.
BC093794 mRNA. Translation: AAH93794.1.
CCDSiCCDS32840.1. [Q96JJ7-1]
RefSeqiNP_061895.3. NM_019022.3. [Q96JJ7-1]
UniGeneiHs.440534.

3D structure databases

ProteinModelPortaliQ96JJ7.
SMRiQ96JJ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119991. 10 interactors.
IntActiQ96JJ7. 20 interactors.
MINTiMINT-3054729.
STRINGi9606.ENSP00000299608.

PTM databases

iPTMnetiQ96JJ7.
PhosphoSitePlusiQ96JJ7.
SwissPalmiQ96JJ7.

Polymorphism and mutation databases

BioMutaiTMX3.
DMDMi78103208.

Proteomic databases

EPDiQ96JJ7.
MaxQBiQ96JJ7.
PaxDbiQ96JJ7.
PeptideAtlasiQ96JJ7.
PRIDEiQ96JJ7.
TopDownProteomicsiQ96JJ7-1. [Q96JJ7-1]

Protocols and materials databases

DNASUi54495.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299608; ENSP00000299608; ENSG00000166479. [Q96JJ7-1]
ENST00000562706; ENSP00000457262; ENSG00000166479. [Q96JJ7-2]
GeneIDi54495.
KEGGihsa:54495.
UCSCiuc002lkf.4. human. [Q96JJ7-1]

Organism-specific databases

CTDi54495.
DisGeNETi54495.
GeneCardsiTMX3.
HGNCiHGNC:24718. TMX3.
HPAiHPA014157.
MIMi616102. gene.
neXtProtiNX_Q96JJ7.
OpenTargetsiENSG00000166479.
PharmGKBiPA164726632.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4277. Eukaryota.
COG0526. LUCA.
GeneTreeiENSGT00860000133691.
HOGENOMiHOG000154655.
InParanoidiQ96JJ7.
KOiK09585.
OMAiMQKRHRV.
OrthoDBiEOG091G0MS9.
PhylomeDBiQ96JJ7.
TreeFamiTF313807.

Enzyme and pathway databases

BioCyciZFISH:HS09404-MONOMER.
ReactomeiR-HSA-114608. Platelet degranulation.

Miscellaneous databases

GeneWikiiTMX3.
GenomeRNAii54495.
PROiQ96JJ7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166479.
CleanExiHS_TXNDC10.
ExpressionAtlasiQ96JJ7. baseline and differential.
GenevisibleiQ96JJ7. HS.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 2 hits.
PROSITEiPS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTMX3_HUMAN
AccessioniPrimary (citable) accession number: Q96JJ7
Secondary accession number(s): B3KV75
, Q52LT7, Q8N5J0, Q9NWJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: November 30, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.