Q96JJ7 (TMX3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein disulfide-isomerase TMX3
EC=5.3.4.1

Alternative name(s):
Thioredoxin domain-containing protein 10
Thioredoxin-related transmembrane protein 3

Gene names

Name:	TMX3
Synonyms:	KIAA1830, TXNDC10

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable disulfide isomerase, which participates in the folding of proteins containing disulfide bonds. May act as a dithiol oxidase. Ref.5
Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins. Ref.5
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein Ref.5.
Tissue specificity	Widely expressed. Expressed in brain, testis, lung, skin, kidney, uterus, bone, stomach, liver, prostate, placenta, eye and muscle. Ref.5
Induction	Not up-regulated by unfolded protein response (UPR). Ref.5
Domain	The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins By similarity.
Post-translational modification	N-glycosylated. Ref.5 Ref.7
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 1 thioredoxin domain.
Biophysicochemical properties	Redox potential: E0 is -157 mV. Ref.5
Sequence caution	The sequence BAA91381.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAB47459.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Redox-active center Signal Transmembrane Transmembrane helix
Molecular function	Isomerase
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glycerol ether metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro protein disulfide isomerase activity Inferred from electronic annotation. Source: EC protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96JJ7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96JJ7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     191-195: YVTLK → VIFKI
     196-454: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 454	430	Protein disulfide-isomerase TMX3		PRO_0000034185
Regions
	Topological domain	25 – 375	351	Lumenal Potential
	Transmembrane	376 – 396	21	Helical; Potential
	Topological domain	397 – 454	58	Cytoplasmic Potential
	Domain	25 – 128	104	Thioredoxin
	Motif	451 – 454	4	Di-lysine motif
Amino acid modifications
	Modified residue	268	1	Phosphoserine Ref.6
	Glycosylation	258	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	313	1	N-linked (GlcNAc...) Potential
	Disulfide bond	53 ↔ 56		Redox-active By similarity
Natural variations
	Alternative sequence	191 – 195	5	YVTLK → VIFKI in isoform 2.		VSP_013748
	Alternative sequence	196 – 454	259	Missing in isoform 2.		VSP_013749
	Natural variant	61	1	P → S. Ref.4 Corresponds to variant rs11557684 [ dbSNP | Ensembl ].		VAR_022451

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: 37EBA0C70ED84A9B	FASTA	454	51,872
	10 20 30 40 50 60 MAAWKSWTAL RLCATVVVLD MVVCKGFVED LDESFKENRN DDIWLVDFYA PWCGHCKKLE 70 80 90 100 110 120 PIWNEVGLEM KSIGSPVKVG KMDATSYSSI ASEFGVRGYP TIKLLKGDLA YNYRGPRTKD 130 140 150 160 170 180 DIIEFAHRVS GALIRPLPSQ QMFEHMQKRH RVFFVYVGGE SPLKEKYIDA ASELIVYTYF 190 200 210 220 230 240 FSASEEVVPE YVTLKEMPAV LVFKDETYFV YDEYEDGDLS SWINRERFQN YLAMDGFLLY 250 260 270 280 290 300 ELGDTGKLVA LAVIDEKNTS VEHTRLKSII QEVARDYRDL FHRDFQFGHM DGNDYINTLL 310 320 330 340 350 360 MDELTVPTVV VLNTSNQQYF LLDRQIKNVE DMVQFINNIL DGTVEAQGGD SILQRLKRIV 370 380 390 400 410 420 FDAKSTIVSI FKSSPLMGCF LFGLPLGVIS IMCYGIYTAD TDGGYIEERY EVSKSENENQ 430 440 450 EQIEESKEQQ EPSSGGSVVP TVQEPKDVLE KKKD
	Isoform 2 [UniParc]. Checksum: 49E7DD4636FF59BE	FASTA	195	22,291
	10 20 30 40 50 60 MAAWKSWTAL RLCATVVVLD MVVCKGFVED LDESFKENRN DDIWLVDFYA PWCGHCKKLE 70 80 90 100 110 120 PIWNEVGLEM KSIGSPVKVG KMDATSYSSI ASEFGVRGYP TIKLLKGDLA YNYRGPRTKD 130 140 150 160 170 180 DIIEFAHRVS GALIRPLPSQ QMFEHMQKRH RVFFVYVGGE SPLKEKYIDA ASELIVYTYF 190 FSASEEVVPE VIFKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O. DNA Res. 8:85-95(2001) [PubMed: 11347906] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon and Tongue.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Skeletal muscle.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT SER-61. Tissue: Brain and Skin.
[5]	"Identification and characterization of a novel thioredoxin-related transmembrane protein of the endoplasmic reticulum." Haugstetter J., Blicher T., Ellgaard L. J. Biol. Chem. 280:8371-8380(2005) [PubMed: 15623505] [Abstract] Cited for: FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, GLYCOSYLATION.
[6]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-258, MASS SPECTROMETRY. Tissue: Liver.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB058733 mRNA. Translation: BAB47459.1. Different initiation. AK000800 mRNA. Translation: BAA91381.1. Different initiation. AK122715 mRNA. Translation: BAG53687.1. BX647846 mRNA. No translation available. BC032325 mRNA. Translation: AAH32325.1. BC093792 mRNA. Translation: AAH93792.1. BC093794 mRNA. Translation: AAH93794.1.
IPI	IPI00064193. IPI00555585.
RefSeq	NP_061895.3. NM_019022.3.
UniGene	Hs.440534.
3D structure databases
ProteinModelPortal	Q96JJ7.
SMR	Q96JJ7. Positions 24-227.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96JJ7. 3 interactions.
STRING	Q96JJ7.
PTM databases
PhosphoSite	Q96JJ7.
Polymorphism databases
DMDM	78103208.
Proteomic databases
PeptideAtlas	Q96JJ7.
PRIDE	Q96JJ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000299608; ENSP00000299608; ENSG00000166479.
GeneID	54495.
KEGG	hsa:54495.
UCSC	uc002lkf.1. human. uc002lkg.2. human.
Organism-specific databases
CTD	54495.
GeneCards	GC18M066340.
H-InvDB	HIX0014509.
HGNC	HGNC:24718. TMX3.
HPA	HPA014157.
neXtProt	NX_Q96JJ7.
PharmGKB	PA134895485. PA164726632.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG11990.
GeneTree	ENSGT00590000082864.
InParanoid	Q96JJ7.
OMA	CYGICTA.
OrthoDB	EOG444KKF.
PhylomeDB	Q96JJ7.
Gene expression databases
ArrayExpress	Q96JJ7.
Bgee	Q96JJ7.
CleanEx	HS_TXNDC10.
Genevestigator	Q96JJ7.
GermOnline	ENSG00000166479. Homo sapiens.
Family and domain databases
InterPro	IPR005746. Thioredoxin. IPR012336. Thioredoxin-like_fold. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KO	K09585.
Pfam	PF00085. Thioredoxin. 1 hit. [Graphical view]
PRINTS	PR00421. THIOREDOXIN.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	56824.

Entry information

Entry name

TMX3_HUMAN

Accession

Primary (citable) accession number: Q96JJ7
Secondary accession number(s): B3KV75 , Q52LT7, Q8N5J0, Q9NWJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	January 25, 2012
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.