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Protein

Ras-associating and dilute domain-containing protein

Gene

RADIL

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Downstream effector of Rap required for cell adhesion and migration of neural crest precursors during development.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

BioCyciZFISH:ENSG00000157927-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-associating and dilute domain-containing protein
Gene namesi
Name:RADIL
Synonyms:KIAA1849
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:22226. RADIL.

Subcellular locationi

GO - Cellular componenti

  • microtubule Source: MGI
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000157927.
PharmGKBiPA164725261.

Polymorphism and mutation databases

BioMutaiRADIL.
DMDMi317373589.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508031 – 1075Ras-associating and dilute domain-containing proteinAdd BLAST1075

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei206PhosphoserineCombined sources1
Modified residuei216PhosphoserineBy similarity1
Modified residuei219PhosphoserineBy similarity1
Modified residuei221PhosphoserineBy similarity1
Modified residuei393PhosphoserineCombined sources1
Modified residuei890PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ96JH8.
PeptideAtlasiQ96JH8.
PRIDEiQ96JH8.

PTM databases

iPTMnetiQ96JH8.
PhosphoSitePlusiQ96JH8.

Expressioni

Gene expression databases

BgeeiENSG00000157927.
ExpressionAtlasiQ96JH8. baseline and differential.
GenevisibleiQ96JH8. HS.

Organism-specific databases

HPAiHPA051776.

Interactioni

Subunit structurei

Interacts with RAP1A; in a GTP-dependent manner. Does not interact with members of the Ras family (PubMed:17704304). Interacts (via PDZ domain) with KIF14; is recruited to the microtubule network restricting its interaction with activated RAP1A (PubMed:23209302).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CEP76Q8TAP63EBI-744267,EBI-742887
GATA1P159763EBI-744267,EBI-3909284
KRT31Q153235EBI-744267,EBI-948001
MAGEA11P43364-23EBI-744267,EBI-10178634
MRFAP1L1Q96HT83EBI-744267,EBI-748896

Protein-protein interaction databases

BioGridi120823. 26 interactors.
IntActiQ96JH8. 9 interactors.
MINTiMINT-1391797.
STRINGi9606.ENSP00000382492.

Structurei

Secondary structure

11075
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi52 – 60Combined sources9
Beta strandi62 – 68Combined sources7
Beta strandi79 – 84Combined sources6
Helixi89 – 99Combined sources11
Helixi104 – 109Combined sources6
Beta strandi110 – 117Combined sources8
Beta strandi130 – 133Combined sources4
Helixi140 – 146Combined sources7
Beta strandi154 – 161Combined sources8
Helixi162 – 188Combined sources27
Beta strandi974 – 980Combined sources7
Beta strandi988 – 992Combined sources5
Turni993 – 995Combined sources3
Beta strandi1001 – 1008Combined sources8
Helixi1013 – 1017Combined sources5
Beta strandi1025 – 1031Combined sources7
Helixi1039 – 1047Combined sources9
Beta strandi1051 – 1058Combined sources8
Helixi1062 – 1068Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UM1NMR-A973-1069[»]
3EC8X-ray2.60A51-193[»]
ProteinModelPortaliQ96JH8.
SMRiQ96JH8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96JH8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 164Ras-associatingPROSITE-ProRule annotationAdd BLAST104
Domaini314 – 389FHAAdd BLAST76
Domaini497 – 764DilutePROSITE-ProRule annotationAdd BLAST268
Domaini976 – 1061PDZPROSITE-ProRule annotationAdd BLAST86

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi847 – 925Pro-richAdd BLAST79

Sequence similaritiesi

Belongs to the RADIL family.Curated
Contains 1 dilute domain.PROSITE-ProRule annotation
Contains 1 FHA domain.Curated
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IQUG. Eukaryota.
ENOG410ZVSZ. LUCA.
GeneTreeiENSGT00530000063674.
HOVERGENiHBG079536.
InParanoidiQ96JH8.
OMAiQDCLVYL.
OrthoDBiEOG091G00X4.
PhylomeDBiQ96JH8.
TreeFamiTF350641.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
2.60.200.20. 1 hit.
InterProiIPR002710. Dilute_dom.
IPR000253. FHA_dom.
IPR001478. PDZ.
IPR000159. RA_dom.
IPR008984. SMAD_FHA_domain.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF01843. DIL. 1 hit.
PF00595. PDZ. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM01132. DIL. 1 hit.
SM00228. PDZ. 1 hit.
SM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS51126. DILUTE. 1 hit.
PS50106. PDZ. 1 hit.
PS50200. RA. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 4 (identifier: Q96JH8-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFYGTHFIMS PPTKSKLKRQ SQLLSSMLSR TLSYKYRDLD STFSSLGASD
60 70 80 90 100
DPAELSTQLS APGVLKVFGD SVCTGTHYKS VLATGTSSAR ELVKEALERY
110 120 130 140 150
ALDPRQAGQY VLCDVVGQAG DAGQRWQARC FRVFGDSEKP LLIQELWKPR
160 170 180 190 200
EGLSRRFELR KRSDVEELAA KEVDTITAGI NAQARRLQRS RAKGTPTPAL
210 220 230 240 250
GDARSSPPPR LRRTVSETSL SPVNALPAAA QGPEEPGPDA MRYSLYQSPH
260 270 280 290 300
LLLLQGYSQQ HDSLVYVLNR DRHTVGQRTP SSKPSISLSA PDILPLHCTI
310 320 330 340 350
RRQPLPDSGQ AAGRLVLEPI PGAHISVNFS EVGHRTVVLH HGDLLSLGLY
360 370 380 390 400
YLLLFKDPAQ AQPLPARALA RLRAVPQSCR LCGAALGARG AASPTQAALP
410 420 430 440 450
RRQQLLLEFE PHLEDTLLQR IMTLIEPGGD DHKLTPAFLL CLCIQHSATH
460 470 480 490 500
FQPGTFGQLL LKIARLIRET VWEKTKELAE KQAQLQEPIS LASCAMADLV
510 520 530 540 550
PDLQPILFWM SNSIELLYFI QQKCPLYMQS MEEQLDITGS KESLFSCTLT
560 570 580 590 600
ASEEAMAVLE EVVLYAFQQC VYYVSKSLYI CLPALLECPP FQTERRESWS
610 620 630 640 650
SAPELPEELR RVVSVYQAAL DLLRQLQVHP EVASQMLAYL FFFSGTLLLN
660 670 680 690 700
QLLDRGPSLS CFHWPRGVQA CARLQQLLEW MRSAGFGAAG EHFFQKLSCT
710 720 730 740 750
LNLLATPRAQ LIQMSWTALR AAFPALSPAQ LHRLLTHYQL ASAMGPMSTW
760 770 780 790 800
EPGAQDSPEA FRSEDVLESY ENPPPIVLPS DGFQVDLEAN CLDDSIYQHL
810 820 830 840 850
LYVRHFLWGL RSRASPGSPG RPGSGASQPV CPEGMHHVVL DGHLEAPSCP
860 870 880 890 900
LAPRDPGPAA REVAPERTLP LRGAPWAQAP PGRQPSRGGS QAGPPHTDSS
910 920 930 940 950
CLLTPPSTPL GPEPGDPDWP ESGGPCGKAL PERQRNGLSG LRGAAPEGDS
960 970 980 990 1000
AALAEESPPA PSSRSSSTED FCYVFTVELE RGPSGLGMGL IDGMHTHLGA
1010 1020 1030 1040 1050
PGLYIQTLLP GSPAAADGRL SLGDRILEVN GSSLLGLGYL RAVDLIRHGG
1060 1070
KKMRFLVAKS DVETAKKIHF RTPPL
Length:1,075
Mass (Da):117,455
Last modified:January 11, 2011 - v5
Checksum:iA071219E8FD10131
GO
Isoform 2 (identifier: Q96JH8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-495: Missing.

Note: No experimental confirmation available.
Show »
Length:580
Mass (Da):63,118
Checksum:iDA26C51AF63798BF
GO
Isoform 3 (identifier: Q96JH8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-704: Missing.
     705-763: ATPRAQLIQM...AQDSPEAFRS → MSPRVGPVTV...GHYGHPLSPA

Note: No experimental confirmation available.
Show »
Length:371
Mass (Da):38,824
Checksum:iFBD298E4B59C5BAB
GO
Isoform 1 (identifier: Q96JH8-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     262-279: DSLVYVLNRDRHTVGQRT → PGVCAQPGPAHGGPAD

Show »
Length:1,073
Mass (Da):116,757
Checksum:iBE16FDC66C40E658
GO

Sequence cautioni

The sequence AAS07559 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAA91543 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB47478 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti700T → I in CAB66665 (PubMed:11230166).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_046192239D → N.Combined sources2 PublicationsCorresponds to variant rs3763384dbSNPEnsembl.1
Natural variantiVAR_046193412H → D.3 PublicationsCorresponds to variant rs2292498dbSNPEnsembl.1
Natural variantiVAR_023769886S → G.7 PublicationsCorresponds to variant rs414035dbSNPEnsembl.1
Natural variantiVAR_060193938L → P.1 PublicationCorresponds to variant rs6966329dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0160971 – 704Missing in isoform 3. 1 PublicationAdd BLAST704
Alternative sequenceiVSP_0160981 – 495Missing in isoform 2. 1 PublicationAdd BLAST495
Alternative sequenceiVSP_035179262 – 279DSLVY…VGQRT → PGVCAQPGPAHGGPAD in isoform 1. 3 PublicationsAdd BLAST18
Alternative sequenceiVSP_016099705 – 763ATPRA…EAFRS → MSPRVGPVTVARIPASCVHR AGDSGLNLLAPGFPHRWAGR PGEGGSFSCGHYGHPLSPA in isoform 3. 1 PublicationAdd BLAST59

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058752 mRNA. Translation: BAB47478.1. Different initiation.
AK000997 mRNA. Translation: BAA91459.1.
AK001186 mRNA. Translation: BAA91543.1. Different initiation.
EF560723 mRNA. Translation: ABQ59033.1.
AC092610 Genomic DNA. Translation: AAS07559.1. Sequence problems.
CH236953 Genomic DNA. Translation: EAL23966.1.
CH471144 Genomic DNA. Translation: EAW87301.1.
BC004919 mRNA. Translation: AAH04919.2.
BC117317 mRNA. Translation: AAI17318.1.
BC126311 mRNA. Translation: AAI26312.1.
BC143526 mRNA. Translation: AAI43527.1.
AL136731 mRNA. Translation: CAB66665.2.
CCDSiCCDS43544.1. [Q96JH8-4]
RefSeqiNP_060529.4. NM_018059.4. [Q96JH8-4]
UniGeneiHs.667336.

Genome annotation databases

EnsembliENST00000399583; ENSP00000382492; ENSG00000157927. [Q96JH8-4]
GeneIDi55698.
KEGGihsa:55698.
UCSCiuc003snj.2. human. [Q96JH8-4]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058752 mRNA. Translation: BAB47478.1. Different initiation.
AK000997 mRNA. Translation: BAA91459.1.
AK001186 mRNA. Translation: BAA91543.1. Different initiation.
EF560723 mRNA. Translation: ABQ59033.1.
AC092610 Genomic DNA. Translation: AAS07559.1. Sequence problems.
CH236953 Genomic DNA. Translation: EAL23966.1.
CH471144 Genomic DNA. Translation: EAW87301.1.
BC004919 mRNA. Translation: AAH04919.2.
BC117317 mRNA. Translation: AAI17318.1.
BC126311 mRNA. Translation: AAI26312.1.
BC143526 mRNA. Translation: AAI43527.1.
AL136731 mRNA. Translation: CAB66665.2.
CCDSiCCDS43544.1. [Q96JH8-4]
RefSeqiNP_060529.4. NM_018059.4. [Q96JH8-4]
UniGeneiHs.667336.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UM1NMR-A973-1069[»]
3EC8X-ray2.60A51-193[»]
ProteinModelPortaliQ96JH8.
SMRiQ96JH8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120823. 26 interactors.
IntActiQ96JH8. 9 interactors.
MINTiMINT-1391797.
STRINGi9606.ENSP00000382492.

PTM databases

iPTMnetiQ96JH8.
PhosphoSitePlusiQ96JH8.

Polymorphism and mutation databases

BioMutaiRADIL.
DMDMi317373589.

Proteomic databases

PaxDbiQ96JH8.
PeptideAtlasiQ96JH8.
PRIDEiQ96JH8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000399583; ENSP00000382492; ENSG00000157927. [Q96JH8-4]
GeneIDi55698.
KEGGihsa:55698.
UCSCiuc003snj.2. human. [Q96JH8-4]

Organism-specific databases

CTDi55698.
GeneCardsiRADIL.
H-InvDBHIX0006446.
HGNCiHGNC:22226. RADIL.
HPAiHPA051776.
MIMi611491. gene.
neXtProtiNX_Q96JH8.
OpenTargetsiENSG00000157927.
PharmGKBiPA164725261.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IQUG. Eukaryota.
ENOG410ZVSZ. LUCA.
GeneTreeiENSGT00530000063674.
HOVERGENiHBG079536.
InParanoidiQ96JH8.
OMAiQDCLVYL.
OrthoDBiEOG091G00X4.
PhylomeDBiQ96JH8.
TreeFamiTF350641.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000157927-MONOMER.

Miscellaneous databases

ChiTaRSiRADIL. human.
EvolutionaryTraceiQ96JH8.
GeneWikiiRADIL.
GenomeRNAii55698.
PROiQ96JH8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000157927.
ExpressionAtlasiQ96JH8. baseline and differential.
GenevisibleiQ96JH8. HS.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
2.60.200.20. 1 hit.
InterProiIPR002710. Dilute_dom.
IPR000253. FHA_dom.
IPR001478. PDZ.
IPR000159. RA_dom.
IPR008984. SMAD_FHA_domain.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF01843. DIL. 1 hit.
PF00595. PDZ. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM01132. DIL. 1 hit.
SM00228. PDZ. 1 hit.
SM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS51126. DILUTE. 1 hit.
PS50106. PDZ. 1 hit.
PS50200. RA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRADIL_HUMAN
AccessioniPrimary (citable) accession number: Q96JH8
Secondary accession number(s): A4D1Z5
, A5YM49, B7ZL20, Q0VFZ9, Q75LH3, Q9BSP5, Q9H0M6, Q9NW43, Q9NWC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: January 11, 2011
Last modified: November 30, 2016
This is version 127 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.