ID   MAGD4_HUMAN             Reviewed;         741 AA.
AC   Q96JG8; A8K093; Q5HYN6; Q8WXW4; Q9BQ84; Q9BVH1; Q9BYH3; Q9BYH4; Q9H217;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   16-JAN-2004, sequence version 3.
DT   27-MAR-2024, entry version 164.
DE   RecName: Full=Melanoma-associated antigen D4;
DE   AltName: Full=MAGE-D4 antigen;
DE   AltName: Full=MAGE-E1 antigen;
GN   Name=MAGED4; Synonyms=KIAA1859, MAGED4A, MAGEE1;
GN   and
GN   Name=MAGED4B;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), AND TISSUE SPECIFICITY.
RX   PubMed=11406556;
RA   Sasaki M., Nakahira K., Kawano Y., Katakura H., Yoshimine T., Shimizu K.,
RA   Kim S.U., Ikenaka K.;
RT   "MAGE-E1, a new member of the melanoma-associated antigen gene family and
RT   its expression in human glioma.";
RL   Cancer Res. 61:4809-4814(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1; 2 AND 3).
RX   PubMed=11602350; DOI=10.1016/s0378-1119(01)00698-9;
RA   Kawano Y., Sasaki M., Nakahira K., Yoshimine T., Shimizu K., Wada H.,
RA   Ikenaka K.;
RT   "Structural characterization and chromosomal localization of the MAGE-E1
RT   gene.";
RL   Gene 277:129-137(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Amygdala;
RX   PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA   Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA   Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA   Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA   Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA   Klein M., Poustka A.;
RT   "Towards a catalog of human genes and proteins: sequencing and analysis of
RT   500 novel complete protein coding human cDNAs.";
RL   Genome Res. 11:422-435(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Brain;
RX   PubMed=11347906; DOI=10.1093/dnares/8.2.85;
RA   Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.;
RT   "Prediction of the coding sequences of unidentified human genes. XX. The
RT   complete sequences of 100 new cDNA clones from brain which code for large
RT   proteins in vitro.";
RL   DNA Res. 8:85-95(2001).
RN   [5]
RP   SEQUENCE REVISION.
RX   PubMed=12168954; DOI=10.1093/dnares/9.3.99;
RA   Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.;
RT   "Construction of expression-ready cDNA clones for KIAA genes: manual
RT   curation of 330 KIAA cDNA clones.";
RL   DNA Res. 9:99-106(2002).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15772651; DOI=10.1038/nature03440;
RA   Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA   Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA   Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA   Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA   Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA   Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA   Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA   Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA   Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA   Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA   Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA   Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA   Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA   Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA   Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA   Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA   Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA   Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA   Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA   Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA   Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA   Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA   Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA   Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA   Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA   Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA   Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA   Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA   Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA   Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA   McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA   Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA   Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA   Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA   Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA   Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA   Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA   Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA   Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA   Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA   d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA   Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA   Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA   Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA   Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA   Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA   Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA   Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA   Rogers J., Bentley D.R.;
RT   "The DNA sequence of the human X chromosome.";
RL   Nature 434:325-337(2005).
RN   [8]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [9]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
RC   TISSUE=Brain, Eye, and PNS;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [10]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 60-741.
RA   Zhang C.;
RT   "Identification of a novel member of human Mage-D subfamily genes.";
RL   Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases.
RN   [11]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 497-627.
RC   TISSUE=Testis;
RA   Lucas S., Boon T.;
RT   "Identification of new genes of the MAGE family.";
RL   Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
RN   [12]
RP   TISSUE SPECIFICITY.
RX   PubMed=16082191; DOI=10.4161/cbt.4.9.1907;
RA   Kraemer B.F., Schoor O., Krueger T., Reichle C., Mueller M., Weinschenk T.,
RA   Hennenlotter J., Stenzl A., Rammensee H.-G., Stevanovic S.;
RT   "MAGED4-expression in renal cell carcinoma and identification of an HLA-
RT   A*25-restricted MHC class I ligand from solid tumor tissue.";
RL   Cancer Biol. Ther. 4:943-948(2005).
RN   [13]
RP   FUNCTION, AND INTERACTION WITH TRIM27.
RX   PubMed=20864041; DOI=10.1016/j.molcel.2010.08.029;
RA   Doyle J.M., Gao J., Wang J., Yang M., Potts P.R.;
RT   "MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases.";
RL   Mol. Cell 39:963-974(2010).
CC   -!- FUNCTION: May enhance ubiquitin ligase activity of RING-type zinc
CC       finger-containing E3 ubiquitin-protein ligases. Proposed to act through
CC       recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at
CC       the E3:substrate complex. {ECO:0000269|PubMed:20864041}.
CC   -!- SUBUNIT: Interacts with TRIM27. {ECO:0000269|PubMed:20864041}.
CC   -!- INTERACTION:
CC       Q96JG8; Q8N111: CEND1; NbExp=3; IntAct=EBI-721864, EBI-946825;
CC       Q96JG8; Q9NVL1-2: FAM86C1P; NbExp=3; IntAct=EBI-721864, EBI-12845222;
CC       Q96JG8; Q0VD86: INCA1; NbExp=3; IntAct=EBI-721864, EBI-6509505;
CC       Q96JG8; P80188: LCN2; NbExp=3; IntAct=EBI-721864, EBI-11911016;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=4;
CC       Name=1; Synonyms=MAGE-E1a;
CC         IsoId=Q96JG8-1; Sequence=Displayed;
CC       Name=2; Synonyms=MAGE-E1b;
CC         IsoId=Q96JG8-2; Sequence=VSP_009287;
CC       Name=3; Synonyms=MAGE-E1c;
CC         IsoId=Q96JG8-3; Sequence=VSP_009288, VSP_009289;
CC       Name=4;
CC         IsoId=Q96JG8-4; Sequence=VSP_035013;
CC   -!- TISSUE SPECIFICITY: Expressed only in brain and ovary among normal
CC       tissues. Isoform 1 and isoform 2 are specifically expressed in glioma
CC       cells among cancer cells. Detected in some renal cell carcinoma
CC       samples. {ECO:0000269|PubMed:11406556, ECO:0000269|PubMed:16082191}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH01207.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAL50032.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=BAB47488.2; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB040527; BAB33378.1; -; mRNA.
DR   EMBL; AB040528; BAB33379.1; -; mRNA.
DR   EMBL; AB040529; BAB33380.1; -; mRNA.
DR   EMBL; AB056059; BAB61778.1; -; Genomic_DNA.
DR   EMBL; AB056059; BAB61779.1; -; Genomic_DNA.
DR   EMBL; AB056059; BAB61780.1; -; Genomic_DNA.
DR   EMBL; AL136582; CAB66517.1; -; mRNA.
DR   EMBL; AB058762; BAB47488.2; ALT_INIT; mRNA.
DR   EMBL; AK289458; BAF82147.1; -; mRNA.
DR   EMBL; AL928717; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BX537154; CAI41307.1; -; Genomic_DNA.
DR   EMBL; CH471230; EAW62885.1; -; Genomic_DNA.
DR   EMBL; BC001207; AAH01207.1; ALT_INIT; mRNA.
DR   EMBL; BC063838; AAH63838.1; -; mRNA.
DR   EMBL; BC081566; AAH81566.1; -; mRNA.
DR   EMBL; AF329733; AAL50032.1; ALT_FRAME; mRNA.
DR   EMBL; AF320908; AAG38604.1; -; mRNA.
DR   CCDS; CCDS14338.1; -. [Q96JG8-1]
DR   CCDS; CCDS35281.1; -. [Q96JG8-2]
DR   CCDS; CCDS48116.1; -. [Q96JG8-2]
DR   CCDS; CCDS56602.1; -. [Q96JG8-4]
DR   CCDS; CCDS65261.1; -. [Q96JG8-4]
DR   CCDS; CCDS94608.1; -. [Q96JG8-1]
DR   RefSeq; NP_001092270.1; NM_001098800.2. [Q96JG8-2]
DR   RefSeq; NP_001229291.1; NM_001242362.1. [Q96JG8-4]
DR   RefSeq; NP_001258990.1; NM_001272061.1. [Q96JG8-4]
DR   RefSeq; NP_001258991.1; NM_001272062.1. [Q96JG8-1]
DR   RefSeq; NP_001258992.1; NM_001272063.1. [Q96JG8-1]
DR   RefSeq; NP_110428.2; NM_030801.3. [Q96JG8-1]
DR   RefSeq; NP_803879.1; NM_177535.2. [Q96JG8-1]
DR   RefSeq; NP_803881.1; NM_177537.2. [Q96JG8-2]
DR   RefSeq; XP_006724670.1; XM_006724607.3.
DR   RefSeq; XP_006724671.1; XM_006724608.3.
DR   RefSeq; XP_011529124.1; XM_011530822.1.
DR   RefSeq; XP_016885365.1; XM_017029876.1.
DR   AlphaFoldDB; Q96JG8; -.
DR   SMR; Q96JG8; -.
DR   BioGRID; 123521; 24.
DR   BioGRID; 608671; 8.
DR   IntAct; Q96JG8; 15.
DR   MINT; Q96JG8; -.
DR   STRING; 9606.ENSP00000421861; -.
DR   iPTMnet; Q96JG8; -.
DR   PhosphoSitePlus; Q96JG8; -.
DR   BioMuta; MAGED4B; -.
DR   DMDM; 41017391; -.
DR   EPD; Q96JG8; -.
DR   jPOST; Q96JG8; -.
DR   MassIVE; Q96JG8; -.
DR   MaxQB; Q96JG8; -.
DR   PaxDb; 9606-ENSP00000421861; -.
DR   PeptideAtlas; Q96JG8; -.
DR   ProteomicsDB; 76958; -. [Q96JG8-1]
DR   ProteomicsDB; 76959; -. [Q96JG8-2]
DR   ProteomicsDB; 76960; -. [Q96JG8-3]
DR   ProteomicsDB; 76961; -. [Q96JG8-4]
DR   Antibodypedia; 1173; 85 antibodies from 15 providers.
DR   Antibodypedia; 26378; 26 antibodies from 9 providers.
DR   DNASU; 81557; -.
DR   Ensembl; ENST00000335504.10; ENSP00000335385.5; ENSG00000187243.17. [Q96JG8-1]
DR   Ensembl; ENST00000360134.10; ENSP00000353252.6; ENSG00000187243.17. [Q96JG8-2]
DR   Ensembl; ENST00000375626.7; ENSP00000364777.3; ENSG00000154545.17. [Q96JG8-2]
DR   Ensembl; ENST00000471932.6; ENSP00000507908.1; ENSG00000154545.17. [Q96JG8-1]
DR   Ensembl; ENST00000485287.5; ENSP00000421861.1; ENSG00000187243.17. [Q96JG8-4]
DR   Ensembl; ENST00000497164.5; ENSP00000423848.1; ENSG00000187243.17. [Q96JG8-1]
DR   Ensembl; ENST00000599522.7; ENSP00000472118.2; ENSG00000154545.17. [Q96JG8-4]
DR   GeneID; 728239; -.
DR   GeneID; 81557; -.
DR   KEGG; hsa:728239; -.
DR   KEGG; hsa:81557; -.
DR   MANE-Select; ENST00000335504.10; ENSP00000335385.5; NM_030801.5; NP_110428.2.
DR   MANE-Select; ENST00000471932.6; ENSP00000507908.1; NM_001272063.2; NP_001258992.1.
DR   UCSC; uc004dpr.5; human. [Q96JG8-1]
DR   AGR; HGNC:22880; -.
DR   AGR; HGNC:23793; -.
DR   CTD; 728239; -.
DR   CTD; 81557; -.
DR   DisGeNET; 728239; -.
DR   DisGeNET; 81557; -.
DR   GeneCards; MAGED4; -.
DR   GeneCards; MAGED4B; -.
DR   HGNC; HGNC:23793; MAGED4.
DR   HGNC; HGNC:22880; MAGED4B.
DR   HPA; ENSG00000154545; Tissue enhanced (brain, ovary).
DR   HPA; ENSG00000187243; Tissue enhanced (brain, pituitary gland).
DR   MIM; 300702; gene.
DR   MIM; 300765; gene.
DR   neXtProt; NX_Q96JG8; -.
DR   OpenTargets; ENSG00000154545; -.
DR   PharmGKB; PA145148472; -.
DR   VEuPathDB; HostDB:ENSG00000154545; -.
DR   VEuPathDB; HostDB:ENSG00000187243; -.
DR   eggNOG; KOG4562; Eukaryota.
DR   GeneTree; ENSGT00940000162389; -.
DR   HOGENOM; CLU_370025_0_0_1; -.
DR   InParanoid; Q96JG8; -.
DR   OMA; AEPWTRM; -.
DR   OrthoDB; 3128316at2759; -.
DR   PhylomeDB; Q96JG8; -.
DR   TreeFam; TF352132; -.
DR   PathwayCommons; Q96JG8; -.
DR   SignaLink; Q96JG8; -.
DR   BioGRID-ORCS; 728239; 74 hits in 639 CRISPR screens.
DR   BioGRID-ORCS; 81557; 38 hits in 643 CRISPR screens.
DR   ChiTaRS; MAGED4; human.
DR   GeneWiki; MAGED4B; -.
DR   Pharos; Q96JG8; Tbio.
DR   PRO; PR:Q96JG8; -.
DR   Proteomes; UP000005640; Chromosome X.
DR   RNAct; Q96JG8; Protein.
DR   Bgee; ENSG00000154545; Expressed in cortical plate and 97 other cell types or tissues.
DR   ExpressionAtlas; Q96JG8; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 1.10.10.1200; MAGE homology domain, winged helix WH1 motif; 1.
DR   Gene3D; 1.10.10.1210; MAGE homology domain, winged helix WH2 motif; 1.
DR   InterPro; IPR037445; MAGE.
DR   InterPro; IPR041898; MAGE_WH1.
DR   InterPro; IPR041899; MAGE_WH2.
DR   InterPro; IPR002190; MHD_dom.
DR   PANTHER; PTHR11736:SF37; MELANOMA-ASSOCIATED ANTIGEN D4; 1.
DR   PANTHER; PTHR11736; MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN; 1.
DR   Pfam; PF01454; MAGE; 1.
DR   SMART; SM01373; MAGE; 1.
DR   PROSITE; PS50838; MAGE; 1.
DR   Genevisible; Q96JG8; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Reference proteome; Tumor antigen;
KW   Ubl conjugation pathway.
FT   CHAIN           1..741
FT                   /note="Melanoma-associated antigen D4"
FT                   /id="PRO_0000156728"
FT   DOMAIN          413..611
FT                   /note="MAGE"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00127"
FT   REGION          1..27
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          136..206
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          247..296
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          323..379
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          700..720
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..15
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        136..155
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        167..206
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        340..367
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         349..414
FT                   /note="SKHLDDEYESSEEERETPAVPPTWRASQPSLTVRAQLAPRPPMAPRSQIPSR
FT                   HVLCLPPRNVTLLQ -> VRSPCPLPPPHPLAPVLSFSSLSCSSPPSPLPLLPLFSSFP
FT                   SFSPHLPSPPLLSSQLVHVSPTQVC (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:11406556"
FT                   /id="VSP_009288"
FT   VAR_SEQ         415..741
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:11406556"
FT                   /id="VSP_009289"
FT   VAR_SEQ         557
FT                   /note="K -> KNPELREETPLSMAPSW (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_035013"
FT   VAR_SEQ         740..741
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:11406556"
FT                   /id="VSP_009287"
FT   CONFLICT        314
FT                   /note="A -> V (in Ref. 6; BAF82147)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        635
FT                   /note="Q -> R (in Ref. 10)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   741 AA;  81378 MW;  56B073324C12A8EC CRC64;
     MAEGSFSVQS ESYSVEDMDE GSDEVGEEEM VEGNDYEEFG AFGGYGTLTS FDIHILRAFG
     SLGPGLRILS NEPWELENPV LAQTLVEALQ LDPETLANET AARAANVARA AASNRAARAA
     AAAARTAFSQ VVASHRVATP QVSGEDTQPT TYAAEAQGPT PEPPLASPQT SQMLVTSKMA
     APEAPATSAQ SQTGSPAQEA ATEGPSSACA FSQAPCAREV DANRPSTAFL GQNDVFDFTQ
     PAGVSGMAFP RPKRPAPAQE AATEGPSAAS GVPQTGPGRE VAATRPKTTK SGKALAKTRW
     VEPQNVVAAA AAKAKMATSI PEPEGAAAAT AQHSAEPWAR MGGKRTKKSK HLDDEYESSE
     EERETPAVPP TWRASQPSLT VRAQLAPRPP MAPRSQIPSR HVLCLPPRNV TLLQERANKL
     VKYLMIKDYK KIPIKRADML KDVIREYDEH FPEIIERATY TLEKKFGIHL KEIDKEEHLY
     ILVCTRDSSA RLLGKTKDTP RLSLLLVILG VIFMNGNRAS EAVLWEALRK MGLRPGVRHP
     FLGDLRKLIT DDFVKQKYLE YKKIPNSNPP EYEFLWGLRA RHETSKMRVL RFIAQNQNRD
     PREWKAHFLE AVDDAFKTMD VDMAEEHARA QMRAQMNIGD EALIGRWSWD DIQVELLTWD
     EDGDFGDAWA RIPFAFWARY HQYILNSNRA NRRATWRAGV SSGTNGGAST SVLDGPSTSS
     TIRTRNAARA GASFFSWIQH R
//