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Protein

Melanoma-associated antigen D4

Gene

MAGED4

more
Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.1 Publication

Keywords - Molecular functioni

Tumor antigen

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:ENSG00000154545-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma-associated antigen D4
Alternative name(s):
MAGE-D4 antigen
MAGE-E1 antigen
Gene namesi
Name:MAGED4
Synonyms:KIAA1859, MAGED4A, MAGEE1
AND
Name:MAGED4B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:23793. MAGED4.
HGNC:22880. MAGED4B.

Pathology & Biotechi

Organism-specific databases

DisGeNETi728239.
81557.
OpenTargetsiENSG00000154545.
ENSG00000187243.
PharmGKBiPA145148472.

Polymorphism and mutation databases

BioMutaiMAGED4.
DMDMi41017391.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001567281 – 741Melanoma-associated antigen D4Add BLAST741

Proteomic databases

EPDiQ96JG8.
MaxQBiQ96JG8.
PaxDbiQ96JG8.
PeptideAtlasiQ96JG8.
PRIDEiQ96JG8.

PTM databases

iPTMnetiQ96JG8.
PhosphoSitePlusiQ96JG8.

Expressioni

Tissue specificityi

Expressed only in brain and ovary among normal tissues. Isoform 1 and isoform 2 are specifically expressed in glioma cells among cancer cells. Detected in some renal cell carcinoma samples.2 Publications

Gene expression databases

BgeeiENSG00000154545.
CleanExiHS_MAGED4.
HS_MAGEE1.
ExpressionAtlasiQ96JG8. baseline and differential.
GenevisibleiQ96JG8. HS.

Organism-specific databases

HPAiHPA003554.

Interactioni

Subunit structurei

Interacts with TRIM27.1 Publication

Protein-protein interaction databases

BioGridi123521. 11 interactors.
608671. 1 interactor.
IntActiQ96JG8. 6 interactors.
MINTiMINT-8247559.
STRINGi9606.ENSP00000364777.

Structurei

3D structure databases

ProteinModelPortaliQ96JG8.
SMRiQ96JG8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini413 – 611MAGEPROSITE-ProRule annotationAdd BLAST199

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi97 – 339Ala-richAdd BLAST243

Sequence similaritiesi

Contains 1 MAGE domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOVERGENiHBG003714.
InParanoidiQ96JG8.
OMAiRTSASFF.
OrthoDBiEOG091G09D3.
PhylomeDBiQ96JG8.
TreeFamiTF352132.

Family and domain databases

InterProiIPR002190. MHD_dom.
[Graphical view]
PfamiPF01454. MAGE. 2 hits.
[Graphical view]
SMARTiSM01373. MAGE. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96JG8-1) [UniParc]FASTAAdd to basket
Also known as: MAGE-E1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEGSFSVQS ESYSVEDMDE GSDEVGEEEM VEGNDYEEFG AFGGYGTLTS
60 70 80 90 100
FDIHILRAFG SLGPGLRILS NEPWELENPV LAQTLVEALQ LDPETLANET
110 120 130 140 150
AARAANVARA AASNRAARAA AAAARTAFSQ VVASHRVATP QVSGEDTQPT
160 170 180 190 200
TYAAEAQGPT PEPPLASPQT SQMLVTSKMA APEAPATSAQ SQTGSPAQEA
210 220 230 240 250
ATEGPSSACA FSQAPCAREV DANRPSTAFL GQNDVFDFTQ PAGVSGMAFP
260 270 280 290 300
RPKRPAPAQE AATEGPSAAS GVPQTGPGRE VAATRPKTTK SGKALAKTRW
310 320 330 340 350
VEPQNVVAAA AAKAKMATSI PEPEGAAAAT AQHSAEPWAR MGGKRTKKSK
360 370 380 390 400
HLDDEYESSE EERETPAVPP TWRASQPSLT VRAQLAPRPP MAPRSQIPSR
410 420 430 440 450
HVLCLPPRNV TLLQERANKL VKYLMIKDYK KIPIKRADML KDVIREYDEH
460 470 480 490 500
FPEIIERATY TLEKKFGIHL KEIDKEEHLY ILVCTRDSSA RLLGKTKDTP
510 520 530 540 550
RLSLLLVILG VIFMNGNRAS EAVLWEALRK MGLRPGVRHP FLGDLRKLIT
560 570 580 590 600
DDFVKQKYLE YKKIPNSNPP EYEFLWGLRA RHETSKMRVL RFIAQNQNRD
610 620 630 640 650
PREWKAHFLE AVDDAFKTMD VDMAEEHARA QMRAQMNIGD EALIGRWSWD
660 670 680 690 700
DIQVELLTWD EDGDFGDAWA RIPFAFWARY HQYILNSNRA NRRATWRAGV
710 720 730 740
SSGTNGGAST SVLDGPSTSS TIRTRNAARA GASFFSWIQH R
Length:741
Mass (Da):81,378
Last modified:January 16, 2004 - v3
Checksum:i56B073324C12A8EC
GO
Isoform 2 (identifier: Q96JG8-2) [UniParc]FASTAAdd to basket
Also known as: MAGE-E1b

The sequence of this isoform differs from the canonical sequence as follows:
     740-741: Missing.

Show »
Length:739
Mass (Da):81,084
Checksum:iA3324C12A8EC3BAB
GO
Isoform 3 (identifier: Q96JG8-3) [UniParc]FASTAAdd to basket
Also known as: MAGE-E1c

The sequence of this isoform differs from the canonical sequence as follows:
     349-414: SKHLDDEYES...LPPRNVTLLQ → VRSPCPLPPP...LVHVSPTQVC
     415-741: Missing.

Show »
Length:414
Mass (Da):42,774
Checksum:i9E9B0351E0B580D8
GO
Isoform 4 (identifier: Q96JG8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     557-557: K → KNPELREETPLSMAPSW

Note: No experimental confirmation available.
Show »
Length:757
Mass (Da):83,217
Checksum:iC52971EDE25D26F0
GO

Sequence cautioni

The sequence AAH01207 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAL50032 differs from that shown. Reason: Frameshift at positions 186 and 219.Curated
The sequence BAB47488 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti314A → V in BAF82147 (PubMed:14702039).Curated1
Sequence conflicti635Q → R (Ref. 10) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009288349 – 414SKHLD…VTLLQ → VRSPCPLPPPHPLAPVLSFS SLSCSSPPSPLPLLPLFSSF PSFSPHLPSPPLLSSQLVHV SPTQVC in isoform 3. 1 PublicationAdd BLAST66
Alternative sequenceiVSP_009289415 – 741Missing in isoform 3. 1 PublicationAdd BLAST327
Alternative sequenceiVSP_035013557K → KNPELREETPLSMAPSW in isoform 4. 1 Publication1
Alternative sequenceiVSP_009287740 – 741Missing in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040527 mRNA. Translation: BAB33378.1.
AB040528 mRNA. Translation: BAB33379.1.
AB040529 mRNA. Translation: BAB33380.1.
AB056059 Genomic DNA. Translation: BAB61778.1.
AB056059 Genomic DNA. Translation: BAB61779.1.
AB056059 Genomic DNA. Translation: BAB61780.1.
AL136582 mRNA. Translation: CAB66517.1.
AB058762 mRNA. Translation: BAB47488.2. Different initiation.
AK289458 mRNA. Translation: BAF82147.1.
AL928717 Genomic DNA. Translation: CAI40426.1.
BX537154 Genomic DNA. Translation: CAI41307.1.
CH471230 Genomic DNA. Translation: EAW62885.1.
BC001207 mRNA. Translation: AAH01207.1. Different initiation.
BC063838 mRNA. Translation: AAH63838.1.
BC081566 mRNA. Translation: AAH81566.1.
AF329733 mRNA. Translation: AAL50032.1. Frameshift.
AF320908 mRNA. Translation: AAG38604.1.
CCDSiCCDS14338.1. [Q96JG8-1]
CCDS35281.1. [Q96JG8-2]
CCDS48116.1. [Q96JG8-2]
CCDS56602.1. [Q96JG8-4]
CCDS65261.1. [Q96JG8-4]
RefSeqiNP_001092270.1. NM_001098800.2. [Q96JG8-2]
NP_001229291.1. NM_001242362.1. [Q96JG8-4]
NP_001258990.1. NM_001272061.1. [Q96JG8-4]
NP_001258991.1. NM_001272062.1. [Q96JG8-1]
NP_001258992.1. NM_001272063.1. [Q96JG8-1]
NP_110428.2. NM_030801.3. [Q96JG8-1]
NP_803879.1. NM_177535.2. [Q96JG8-1]
NP_803881.1. NM_177537.2. [Q96JG8-2]
XP_006724670.1. XM_006724607.3. [Q96JG8-1]
XP_006724671.1. XM_006724608.3. [Q96JG8-1]
XP_011529124.1. XM_011530822.1. [Q96JG8-2]
XP_016885365.1. XM_017029876.1. [Q96JG8-2]
UniGeneiHs.522650.
Hs.571729.
Hs.733143.

Genome annotation databases

EnsembliENST00000335504; ENSP00000335385; ENSG00000187243. [Q96JG8-1]
ENST00000360134; ENSP00000353252; ENSG00000187243. [Q96JG8-2]
ENST00000375626; ENSP00000364777; ENSG00000154545. [Q96JG8-2]
ENST00000485287; ENSP00000421861; ENSG00000187243. [Q96JG8-4]
ENST00000497164; ENSP00000423848; ENSG00000187243. [Q96JG8-1]
ENST00000599522; ENSP00000472118; ENSG00000154545. [Q96JG8-4]
GeneIDi728239.
81557.
KEGGihsa:728239.
hsa:81557.
UCSCiuc004dpr.5. human. [Q96JG8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040527 mRNA. Translation: BAB33378.1.
AB040528 mRNA. Translation: BAB33379.1.
AB040529 mRNA. Translation: BAB33380.1.
AB056059 Genomic DNA. Translation: BAB61778.1.
AB056059 Genomic DNA. Translation: BAB61779.1.
AB056059 Genomic DNA. Translation: BAB61780.1.
AL136582 mRNA. Translation: CAB66517.1.
AB058762 mRNA. Translation: BAB47488.2. Different initiation.
AK289458 mRNA. Translation: BAF82147.1.
AL928717 Genomic DNA. Translation: CAI40426.1.
BX537154 Genomic DNA. Translation: CAI41307.1.
CH471230 Genomic DNA. Translation: EAW62885.1.
BC001207 mRNA. Translation: AAH01207.1. Different initiation.
BC063838 mRNA. Translation: AAH63838.1.
BC081566 mRNA. Translation: AAH81566.1.
AF329733 mRNA. Translation: AAL50032.1. Frameshift.
AF320908 mRNA. Translation: AAG38604.1.
CCDSiCCDS14338.1. [Q96JG8-1]
CCDS35281.1. [Q96JG8-2]
CCDS48116.1. [Q96JG8-2]
CCDS56602.1. [Q96JG8-4]
CCDS65261.1. [Q96JG8-4]
RefSeqiNP_001092270.1. NM_001098800.2. [Q96JG8-2]
NP_001229291.1. NM_001242362.1. [Q96JG8-4]
NP_001258990.1. NM_001272061.1. [Q96JG8-4]
NP_001258991.1. NM_001272062.1. [Q96JG8-1]
NP_001258992.1. NM_001272063.1. [Q96JG8-1]
NP_110428.2. NM_030801.3. [Q96JG8-1]
NP_803879.1. NM_177535.2. [Q96JG8-1]
NP_803881.1. NM_177537.2. [Q96JG8-2]
XP_006724670.1. XM_006724607.3. [Q96JG8-1]
XP_006724671.1. XM_006724608.3. [Q96JG8-1]
XP_011529124.1. XM_011530822.1. [Q96JG8-2]
XP_016885365.1. XM_017029876.1. [Q96JG8-2]
UniGeneiHs.522650.
Hs.571729.
Hs.733143.

3D structure databases

ProteinModelPortaliQ96JG8.
SMRiQ96JG8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123521. 11 interactors.
608671. 1 interactor.
IntActiQ96JG8. 6 interactors.
MINTiMINT-8247559.
STRINGi9606.ENSP00000364777.

PTM databases

iPTMnetiQ96JG8.
PhosphoSitePlusiQ96JG8.

Polymorphism and mutation databases

BioMutaiMAGED4.
DMDMi41017391.

Proteomic databases

EPDiQ96JG8.
MaxQBiQ96JG8.
PaxDbiQ96JG8.
PeptideAtlasiQ96JG8.
PRIDEiQ96JG8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000335504; ENSP00000335385; ENSG00000187243. [Q96JG8-1]
ENST00000360134; ENSP00000353252; ENSG00000187243. [Q96JG8-2]
ENST00000375626; ENSP00000364777; ENSG00000154545. [Q96JG8-2]
ENST00000485287; ENSP00000421861; ENSG00000187243. [Q96JG8-4]
ENST00000497164; ENSP00000423848; ENSG00000187243. [Q96JG8-1]
ENST00000599522; ENSP00000472118; ENSG00000154545. [Q96JG8-4]
GeneIDi728239.
81557.
KEGGihsa:728239.
hsa:81557.
UCSCiuc004dpr.5. human. [Q96JG8-1]

Organism-specific databases

CTDi728239.
81557.
DisGeNETi728239.
81557.
GeneCardsiMAGED4.
MAGED4B.
HGNCiHGNC:23793. MAGED4.
HGNC:22880. MAGED4B.
HPAiHPA003554.
MIMi300702. gene.
300765. gene.
neXtProtiNX_Q96JG8.
OpenTargetsiENSG00000154545.
ENSG00000187243.
PharmGKBiPA145148472.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOVERGENiHBG003714.
InParanoidiQ96JG8.
OMAiRTSASFF.
OrthoDBiEOG091G09D3.
PhylomeDBiQ96JG8.
TreeFamiTF352132.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000154545-MONOMER.

Miscellaneous databases

ChiTaRSiMAGED4B. human.
GeneWikiiMAGED4B.
PROiQ96JG8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000154545.
CleanExiHS_MAGED4.
HS_MAGEE1.
ExpressionAtlasiQ96JG8. baseline and differential.
GenevisibleiQ96JG8. HS.

Family and domain databases

InterProiIPR002190. MHD_dom.
[Graphical view]
PfamiPF01454. MAGE. 2 hits.
[Graphical view]
SMARTiSM01373. MAGE. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAGD4_HUMAN
AccessioniPrimary (citable) accession number: Q96JG8
Secondary accession number(s): A8K093
, Q5HYN6, Q8WXW4, Q9BQ84, Q9BVH1, Q9BYH3, Q9BYH4, Q9H217
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: November 2, 2016
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.