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Protein

Melanoma-associated antigen D4

Gene

MAGED4

more
Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.1 Publication

Caution

Keywordsi

Molecular functionTumor antigen
Biological processUbl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma-associated antigen D4
Alternative name(s):
MAGE-D4 antigen
MAGE-E1 antigen
Gene namesi
Name:MAGED4
Synonyms:KIAA1859, MAGED4A, MAGEE1
AND
Name:MAGED4B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000154545.16
HostDB:ENSG00000187243.16
HGNCiHGNC:23793 MAGED4
HGNC:22880 MAGED4B
MIMi300702 gene
300765 gene
neXtProtiNX_Q96JG8

Pathology & Biotechi

Organism-specific databases

DisGeNETi728239
81557
OpenTargetsiENSG00000154545
PharmGKBiPA145148472

Polymorphism and mutation databases

BioMutaiMAGED4
DMDMi41017391

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001567281 – 741Melanoma-associated antigen D4Add BLAST741

Proteomic databases

EPDiQ96JG8
MaxQBiQ96JG8
PaxDbiQ96JG8
PeptideAtlasiQ96JG8
PRIDEiQ96JG8

PTM databases

iPTMnetiQ96JG8
PhosphoSitePlusiQ96JG8

Expressioni

Tissue specificityi

Expressed only in brain and ovary among normal tissues. Isoform 1 and isoform 2 are specifically expressed in glioma cells among cancer cells. Detected in some renal cell carcinoma samples.2 Publications

Gene expression databases

BgeeiENSG00000154545
CleanExiHS_MAGED4
HS_MAGEE1
ExpressionAtlasiQ96JG8 baseline and differential
GenevisibleiQ96JG8 HS

Organism-specific databases

HPAiHPA003554

Interactioni

Subunit structurei

Interacts with TRIM27.1 Publication

Protein-protein interaction databases

BioGridi12352114 interactors.
6086712 interactors.
IntActiQ96JG8 6 interactors.
MINTiQ96JG8
STRINGi9606.ENSP00000364777

Structurei

3D structure databases

ProteinModelPortaliQ96JG8
SMRiQ96JG8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini413 – 611MAGEPROSITE-ProRule annotationAdd BLAST199

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi97 – 339Ala-richAdd BLAST243

Phylogenomic databases

eggNOGiKOG4562 Eukaryota
ENOG4111S70 LUCA
GeneTreeiENSGT00760000118824
HOVERGENiHBG003714
InParanoidiQ96JG8
OMAiCANECKF
OrthoDBiEOG091G09D3
PhylomeDBiQ96JG8
TreeFamiTF352132

Family and domain databases

InterProiView protein in InterPro
IPR037445 MAGE
IPR030091 MAGED4
IPR002190 MHD_dom
PANTHERiPTHR11736 PTHR11736, 1 hit
PTHR11736:SF37 PTHR11736:SF37, 1 hit
PfamiView protein in Pfam
PF01454 MAGE, 2 hits
SMARTiView protein in SMART
SM01373 MAGE, 1 hit
PROSITEiView protein in PROSITE
PS50838 MAGE, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96JG8-1) [UniParc]FASTAAdd to basket
Also known as: MAGE-E1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEGSFSVQS ESYSVEDMDE GSDEVGEEEM VEGNDYEEFG AFGGYGTLTS
60 70 80 90 100
FDIHILRAFG SLGPGLRILS NEPWELENPV LAQTLVEALQ LDPETLANET
110 120 130 140 150
AARAANVARA AASNRAARAA AAAARTAFSQ VVASHRVATP QVSGEDTQPT
160 170 180 190 200
TYAAEAQGPT PEPPLASPQT SQMLVTSKMA APEAPATSAQ SQTGSPAQEA
210 220 230 240 250
ATEGPSSACA FSQAPCAREV DANRPSTAFL GQNDVFDFTQ PAGVSGMAFP
260 270 280 290 300
RPKRPAPAQE AATEGPSAAS GVPQTGPGRE VAATRPKTTK SGKALAKTRW
310 320 330 340 350
VEPQNVVAAA AAKAKMATSI PEPEGAAAAT AQHSAEPWAR MGGKRTKKSK
360 370 380 390 400
HLDDEYESSE EERETPAVPP TWRASQPSLT VRAQLAPRPP MAPRSQIPSR
410 420 430 440 450
HVLCLPPRNV TLLQERANKL VKYLMIKDYK KIPIKRADML KDVIREYDEH
460 470 480 490 500
FPEIIERATY TLEKKFGIHL KEIDKEEHLY ILVCTRDSSA RLLGKTKDTP
510 520 530 540 550
RLSLLLVILG VIFMNGNRAS EAVLWEALRK MGLRPGVRHP FLGDLRKLIT
560 570 580 590 600
DDFVKQKYLE YKKIPNSNPP EYEFLWGLRA RHETSKMRVL RFIAQNQNRD
610 620 630 640 650
PREWKAHFLE AVDDAFKTMD VDMAEEHARA QMRAQMNIGD EALIGRWSWD
660 670 680 690 700
DIQVELLTWD EDGDFGDAWA RIPFAFWARY HQYILNSNRA NRRATWRAGV
710 720 730 740
SSGTNGGAST SVLDGPSTSS TIRTRNAARA GASFFSWIQH R
Length:741
Mass (Da):81,378
Last modified:January 16, 2004 - v3
Checksum:i56B073324C12A8EC
GO
Isoform 2 (identifier: Q96JG8-2) [UniParc]FASTAAdd to basket
Also known as: MAGE-E1b

The sequence of this isoform differs from the canonical sequence as follows:
     740-741: Missing.

Show »
Length:739
Mass (Da):81,084
Checksum:iA3324C12A8EC3BAB
GO
Isoform 3 (identifier: Q96JG8-3) [UniParc]FASTAAdd to basket
Also known as: MAGE-E1c

The sequence of this isoform differs from the canonical sequence as follows:
     349-414: SKHLDDEYES...LPPRNVTLLQ → VRSPCPLPPP...LVHVSPTQVC
     415-741: Missing.

Show »
Length:414
Mass (Da):42,774
Checksum:i9E9B0351E0B580D8
GO
Isoform 4 (identifier: Q96JG8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     557-557: K → KNPELREETPLSMAPSW

Note: No experimental confirmation available.
Show »
Length:757
Mass (Da):83,217
Checksum:iC52971EDE25D26F0
GO

Sequence cautioni

The sequence AAH01207 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAL50032 differs from that shown. Reason: Frameshift at positions 186 and 219.Curated
The sequence BAB47488 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti314A → V in BAF82147 (PubMed:14702039).Curated1
Sequence conflicti635Q → R (Ref. 10) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009288349 – 414SKHLD…VTLLQ → VRSPCPLPPPHPLAPVLSFS SLSCSSPPSPLPLLPLFSSF PSFSPHLPSPPLLSSQLVHV SPTQVC in isoform 3. 1 PublicationAdd BLAST66
Alternative sequenceiVSP_009289415 – 741Missing in isoform 3. 1 PublicationAdd BLAST327
Alternative sequenceiVSP_035013557K → KNPELREETPLSMAPSW in isoform 4. 1 Publication1
Alternative sequenceiVSP_009287740 – 741Missing in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040527 mRNA Translation: BAB33378.1
AB040528 mRNA Translation: BAB33379.1
AB040529 mRNA Translation: BAB33380.1
AB056059 Genomic DNA Translation: BAB61778.1
AB056059 Genomic DNA Translation: BAB61779.1
AB056059 Genomic DNA Translation: BAB61780.1
AL136582 mRNA Translation: CAB66517.1
AB058762 mRNA Translation: BAB47488.2 Different initiation.
AK289458 mRNA Translation: BAF82147.1
AL928717 Genomic DNA Translation: CAI40426.1
BX537154 Genomic DNA Translation: CAI41307.1
CH471230 Genomic DNA Translation: EAW62885.1
BC001207 mRNA Translation: AAH01207.1 Different initiation.
BC063838 mRNA Translation: AAH63838.1
BC081566 mRNA Translation: AAH81566.1
AF329733 mRNA Translation: AAL50032.1 Frameshift.
AF320908 mRNA Translation: AAG38604.1
CCDSiCCDS14338.1 [Q96JG8-1]
CCDS35281.1 [Q96JG8-2]
CCDS48116.1 [Q96JG8-2]
CCDS56602.1 [Q96JG8-4]
CCDS65261.1 [Q96JG8-4]
RefSeqiNP_001092270.1, NM_001098800.2 [Q96JG8-2]
NP_001229291.1, NM_001242362.1 [Q96JG8-4]
NP_001258990.1, NM_001272061.1 [Q96JG8-4]
NP_001258991.1, NM_001272062.1 [Q96JG8-1]
NP_001258992.1, NM_001272063.1 [Q96JG8-1]
NP_110428.2, NM_030801.3 [Q96JG8-1]
NP_803879.1, NM_177535.2 [Q96JG8-1]
NP_803881.1, NM_177537.2 [Q96JG8-2]
XP_006724670.1, XM_006724607.3 [Q96JG8-1]
XP_006724671.1, XM_006724608.3 [Q96JG8-1]
XP_011529124.1, XM_011530822.1 [Q96JG8-2]
XP_016885365.1, XM_017029876.1 [Q96JG8-2]
UniGeneiHs.522650
Hs.571729
Hs.733143

Genome annotation databases

EnsembliENST00000335504; ENSP00000335385; ENSG00000187243 [Q96JG8-1]
ENST00000360134; ENSP00000353252; ENSG00000187243 [Q96JG8-2]
ENST00000375626; ENSP00000364777; ENSG00000154545 [Q96JG8-2]
ENST00000485287; ENSP00000421861; ENSG00000187243 [Q96JG8-4]
ENST00000497164; ENSP00000423848; ENSG00000187243 [Q96JG8-1]
ENST00000599522; ENSP00000472118; ENSG00000154545 [Q96JG8-4]
GeneIDi728239
81557
KEGGihsa:728239
hsa:81557
UCSCiuc004dpr.5 human [Q96JG8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMAGD4_HUMAN
AccessioniPrimary (citable) accession number: Q96JG8
Secondary accession number(s): A8K093
, Q5HYN6, Q8WXW4, Q9BQ84, Q9BVH1, Q9BYH3, Q9BYH4, Q9H217
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: April 25, 2018
This is version 130 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome