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Q96JF0

- SIAT2_HUMAN

UniProt

Q96JF0 - SIAT2_HUMAN

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Protein

Beta-galactoside alpha-2,6-sialyltransferase 2

Gene

ST6GAL2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. Has alpha-2,6-sialyltransferase activity toward oligosaccharides that have the Gal-beta-1,4-GlcNAc sequence at the non-reducing end of their carbohydrate groups, but it has weak or no activities toward glycoproteins and glycolipids.

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.3 Publications

Kineticsi

  1. KM=0.74 mM for Gal-beta-1,4-GlcNAc-beta-O-octyl2 Publications
  2. KM=0.71 mM for Gal-beta-1,4-GlcNAc2 Publications
  3. KM=0.48 mM for lacto-N-neotetraose2 Publications

GO - Molecular functioni

  1. beta-galactoside alpha-2,6-sialyltransferase activity Source: UniProtKB

GO - Biological processi

  1. growth Source: UniProtKB
  2. multicellular organismal development Source: UniProtKB
  3. oligosaccharide metabolic process Source: UniProtKB
  4. protein glycosylation Source: InterPro
  5. sialylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_200874. Sialic acid metabolism.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactoside alpha-2,6-sialyltransferase 2 (EC:2.4.99.1)
Short name:
Alpha 2,6-ST 2
Alternative name(s):
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 2
ST6Gal II
Short name:
ST6GalII
Short name:
hST6Gal II
Sialyltransferase 2
Gene namesi
Name:ST6GAL2
Synonyms:KIAA1877, SIAT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:10861. ST6GAL2.

Subcellular locationi

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35763.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 529529Beta-galactoside alpha-2,6-sialyltransferase 2PRO_0000314785Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi69 – 691O-linked (GalNAc...)1 Publication
Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi253 ↔ 519By similarity
Disulfide bondi296 ↔ 448By similarity
Disulfide bondi466 ↔ 477By similarity

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ96JF0.
PRIDEiQ96JF0.

PTM databases

PhosphoSiteiQ96JF0.

Expressioni

Tissue specificityi

Weakly expressed in some tissues, such as small intestine, colon and fetal brain.2 Publications

Inductioni

By IL6/interleukin-6 and IL8//interleukin-8.1 Publication

Gene expression databases

BgeeiQ96JF0.
CleanExiHS_ST6GAL2.
ExpressionAtlasiQ96JF0. baseline and differential.
GenevestigatoriQ96JF0.

Organism-specific databases

HPAiHPA014459.

Interactioni

Protein-protein interaction databases

IntActiQ96JF0. 1 interaction.
STRINGi9606.ENSP00000355273.

Structurei

3D structure databases

ProteinModelPortaliQ96JF0.
SMRiQ96JF0. Positions 205-519.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini33 – 529497LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei12 – 3221Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249416.
GeneTreeiENSGT00550000074444.
HOGENOMiHOG000013206.
HOVERGENiHBG052853.
InParanoidiQ96JF0.
KOiK00779.
OMAiYFTDSNP.
PhylomeDBiQ96JF0.
TreeFamiTF323961.

Family and domain databases

InterProiIPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR001675. Glyco_trans_29.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
SUPFAMiSSF88713. SSF88713. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q96JF0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKPHLKQWRQ RMLFGIFAWG LLFLLIFIYF TDSNPAEPVP SSLSFLETRR
60 70 80 90 100
LLPVQGKQRA IMGAAHEPSP PGGLDARQAL PRAHPAGSFH AGPGDLQKWA
110 120 130 140 150
QSQDGFEHKE FFSSQVGRKS QSAFYPEDDD YFFAAGQPGW HSHTQGTLGF
160 170 180 190 200
PSPGEPGPRE GAFPAAQVQR RRVKKRHRRQ RRSHVLEEGD DGDRLYSSMS
210 220 230 240 250
RAFLYRLWKG NVSSKMLNPR LQKAMKDYLT ANKHGVRFRG KREAGLSRAQ
260 270 280 290 300
LLCQLRSRAR VRTLDGTEAP FSALGWRRLV PAVPLSQLHP RGLRSCAVVM
310 320 330 340 350
SAGAILNSSL GEEIDSHDAV LRFNSAPTRG YEKDVGNKTT IRIINSQILT
360 370 380 390 400
NPSHHFIDSS LYKDVILVAW DPAPYSANLN LWYKKPDYNL FTPYIQHRQR
410 420 430 440 450
NPNQPFYILH PKFIWQLWDI IQENTKEKIQ PNPPSSGFIG ILIMMSMCRE
460 470 480 490 500
VHVYEYIPSV RQTELCHYHE LYYDAACTLG AYHPLLYEKL LVQRLNMGTQ
510 520
GDLHRKGKVV LPGFQAVHCP APSPVIPHS
Length:529
Mass (Da):60,158
Last modified:January 15, 2008 - v2
Checksum:iC3647E6DC0A9EFE3
GO
Isoform 2 (identifier: Q96JF0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     441-529: ILIMMSMCRE...PAPSPVIPHS → SFVKIGHIRACSEPRSRDCTPAWTTE

Note: No experimental confirmation available.

Show »
Length:466
Mass (Da):52,927
Checksum:i9180AF089B7A181C
GO

Sequence cautioni

The sequence AAH08680.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence BAB47506.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti154 – 1541G → R.
Corresponds to variant rs3796110 [ dbSNP | Ensembl ].
VAR_038046
Natural varianti341 – 3411I → V.
Corresponds to variant rs12615112 [ dbSNP | Ensembl ].
VAR_038047

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei441 – 52989ILIMM…VIPHS → SFVKIGHIRACSEPRSRDCT PAWTTE in isoform 2. 1 PublicationVSP_030356Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB059555 mRNA. Translation: BAC24793.1.
AB058780 mRNA. Translation: BAB47506.1. Different initiation.
AC016994 Genomic DNA. No translation available.
AC108049 Genomic DNA. Translation: AAY15022.1.
CH471127 Genomic DNA. Translation: EAX01742.1.
CH471127 Genomic DNA. Translation: EAX01743.1.
BC008680 mRNA. Translation: AAH08680.1. Different initiation.
AJ512141 mRNA. Translation: CAD54408.1.
CCDSiCCDS2073.1. [Q96JF0-1]
CCDS46380.1. [Q96JF0-2]
RefSeqiNP_001135823.1. NM_001142351.1. [Q96JF0-1]
NP_001135824.1. NM_001142352.1. [Q96JF0-2]
NP_115917.1. NM_032528.2. [Q96JF0-1]
XP_006712864.1. XM_006712801.1. [Q96JF0-1]
XP_006712865.1. XM_006712802.1. [Q96JF0-1]
UniGeneiHs.98265.

Genome annotation databases

EnsembliENST00000361686; ENSP00000355273; ENSG00000144057. [Q96JF0-1]
ENST00000409087; ENSP00000387332; ENSG00000144057. [Q96JF0-2]
ENST00000409382; ENSP00000386942; ENSG00000144057. [Q96JF0-1]
GeneIDi84620.
KEGGihsa:84620.
UCSCiuc002tdq.3. human. [Q96JF0-1]
uc002tds.3. human. [Q96JF0-2]

Polymorphism databases

DMDMi166219772.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB059555 mRNA. Translation: BAC24793.1 .
AB058780 mRNA. Translation: BAB47506.1 . Different initiation.
AC016994 Genomic DNA. No translation available.
AC108049 Genomic DNA. Translation: AAY15022.1 .
CH471127 Genomic DNA. Translation: EAX01742.1 .
CH471127 Genomic DNA. Translation: EAX01743.1 .
BC008680 mRNA. Translation: AAH08680.1 . Different initiation.
AJ512141 mRNA. Translation: CAD54408.1 .
CCDSi CCDS2073.1. [Q96JF0-1 ]
CCDS46380.1. [Q96JF0-2 ]
RefSeqi NP_001135823.1. NM_001142351.1. [Q96JF0-1 ]
NP_001135824.1. NM_001142352.1. [Q96JF0-2 ]
NP_115917.1. NM_032528.2. [Q96JF0-1 ]
XP_006712864.1. XM_006712801.1. [Q96JF0-1 ]
XP_006712865.1. XM_006712802.1. [Q96JF0-1 ]
UniGenei Hs.98265.

3D structure databases

ProteinModelPortali Q96JF0.
SMRi Q96JF0. Positions 205-519.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q96JF0. 1 interaction.
STRINGi 9606.ENSP00000355273.

Protein family/group databases

CAZyi GT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSitei Q96JF0.

Polymorphism databases

DMDMi 166219772.

Proteomic databases

PaxDbi Q96JF0.
PRIDEi Q96JF0.

Protocols and materials databases

DNASUi 84620.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000361686 ; ENSP00000355273 ; ENSG00000144057 . [Q96JF0-1 ]
ENST00000409087 ; ENSP00000387332 ; ENSG00000144057 . [Q96JF0-2 ]
ENST00000409382 ; ENSP00000386942 ; ENSG00000144057 . [Q96JF0-1 ]
GeneIDi 84620.
KEGGi hsa:84620.
UCSCi uc002tdq.3. human. [Q96JF0-1 ]
uc002tds.3. human. [Q96JF0-2 ]

Organism-specific databases

CTDi 84620.
GeneCardsi GC02M107418.
H-InvDB HIX0161642.
HGNCi HGNC:10861. ST6GAL2.
HPAi HPA014459.
MIMi 608472. gene.
neXtProti NX_Q96JF0.
PharmGKBi PA35763.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG249416.
GeneTreei ENSGT00550000074444.
HOGENOMi HOG000013206.
HOVERGENi HBG052853.
InParanoidi Q96JF0.
KOi K00779.
OMAi YFTDSNP.
PhylomeDBi Q96JF0.
TreeFami TF323961.

Enzyme and pathway databases

Reactomei REACT_200874. Sialic acid metabolism.

Miscellaneous databases

GenomeRNAii 84620.
NextBioi 74504.
PROi Q96JF0.
SOURCEi Search...

Gene expression databases

Bgeei Q96JF0.
CleanExi HS_ST6GAL2.
ExpressionAtlasi Q96JF0. baseline and differential.
Genevestigatori Q96JF0.

Family and domain databases

InterProi IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR001675. Glyco_trans_29.
[Graphical view ]
Pfami PF00777. Glyco_transf_29. 1 hit.
[Graphical view ]
SUPFAMi SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the second type of human beta-galactoside alpha2,6-sialyltransferase (ST6Gal II) that sialylates Galbeta1,4GlcNAc structures on oligosaccharides preferentially."
    Takashima S., Tsuji S., Tsujimoto M.
    J. Biol. Chem. 277:45719-45728(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
  2. "Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.
    DNA Res. 8:85-95(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Ovary.
  6. "Identification and functional expression of a second human beta-galactoside alpha2,6-sialyltransferase, ST6Gal II."
    Krzewinski-Recchi M.-A., Julien S., Juliant S., Teintenier-Lelievre M., Samyn-Petit B., Montiel M.-D., Mir A.-M., Cerutti M., Harduin-Lepers A., Delannoy P.
    Eur. J. Biochem. 270:950-961(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 315-529 (ISOFORM 1), ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
  7. "Probing the substrate specificity of four different sialyltransferases using synthetic beta-D-Galp-(1-->4)-beta-D-GlcpNAc-(1-->2)-alpha-D-Manp-(1-->O) (CH(2))7CH3 analogues general activating effect of replacing N-acetylglucosamine by N-propionylglucosamine."
    Rohfritsch P.F., Joosten J.A.F., Krzewinski-Recchi M.-A., Harduin-Lepers A., Laporte B., Juliant S., Cerutti M., Delannoy P., Vliegenthart J.F.G., Kamerling J.P.
    Biochim. Biophys. Acta 1760:685-692(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME ACTIVITY.
  8. "IL-6 and IL-8 increase the expression of glycosyltransferases and sulfotransferases involved in the biosynthesis of sialylated and/or sulfated Lewis x epitopes in the human bronchial mucosa."
    Groux-Degroote S., Krzewinski-Recchi M.-A., Cazet A., Vincent A., Lehoux S., Lafitte J.-J., van Seuningen I., Delannoy P.
    Biochem. J. 410:213-223(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  9. "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
    Halim A., Ruetschi U., Larson G., Nilsson J.
    J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT SER-69, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiSIAT2_HUMAN
AccessioniPrimary (citable) accession number: Q96JF0
Secondary accession number(s): D3DVK3
, Q53QP4, Q86Y44, Q8IUG7, Q96HE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: October 29, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3