Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q96JF0 (SIAT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactoside alpha-2,6-sialyltransferase 2

Short name=Alpha 2,6-ST 2
EC=2.4.99.1
Alternative name(s):
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 2
ST6Gal II
Short name=ST6GalII
Short name=hST6Gal II
Sialyltransferase 2
Gene names
Name:ST6GAL2
Synonyms:KIAA1877, SIAT2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length529 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. Has alpha-2,6-sialyltransferase activity toward oligosaccharides that have the Gal-beta-1,4-GlcNAc sequence at the non-reducing end of their carbohydrate groups, but it has weak or no activities toward glycoproteins and glycolipids.

Catalytic activity

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine. Ref.1 Ref.6 Ref.7

Subcellular location

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein By similarity.

Tissue specificity

Weakly expressed in some tissues, such as small intestine, colon and fetal brain. Ref.1 Ref.6

Induction

By IL6/interleukin-6 and IL8//interleukin-8. Ref.8

Post-translational modification

O-glycosylated. Ref.9

Sequence similarities

Belongs to the glycosyltransferase 29 family.

Biophysicochemical properties

Kinetic parameters:

KM=0.74 mM for Gal-beta-1,4-GlcNAc-beta-O-octyl Ref.1 Ref.6

KM=0.71 mM for Gal-beta-1,4-GlcNAc

KM=0.48 mM for lacto-N-neotetraose

Sequence caution

The sequence AAH08680.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAB47506.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96JF0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96JF0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     441-529: ILIMMSMCRE...PAPSPVIPHS → SFVKIGHIRACSEPRSRDCTPAWTTE
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 529529Beta-galactoside alpha-2,6-sialyltransferase 2
PRO_0000314785

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 3221Helical; Signal-anchor for type II membrane protein; Potential
Topological domain33 – 529497Lumenal Potential

Amino acid modifications

Glycosylation691O-linked (GalNAc...) Ref.9
Glycosylation2111N-linked (GlcNAc...) Potential
Disulfide bond253 ↔ 519 By similarity
Disulfide bond296 ↔ 448 By similarity
Disulfide bond466 ↔ 477 By similarity

Natural variations

Alternative sequence441 – 52989ILIMM…VIPHS → SFVKIGHIRACSEPRSRDCT PAWTTE in isoform 2.
VSP_030356
Natural variant1541G → R.
Corresponds to variant rs3796110 [ dbSNP | Ensembl ].
VAR_038046
Natural variant3411I → V.
Corresponds to variant rs12615112 [ dbSNP | Ensembl ].
VAR_038047

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 15, 2008. Version 2.
Checksum: C3647E6DC0A9EFE3

FASTA52960,158
        10         20         30         40         50         60 
MKPHLKQWRQ RMLFGIFAWG LLFLLIFIYF TDSNPAEPVP SSLSFLETRR LLPVQGKQRA 

        70         80         90        100        110        120 
IMGAAHEPSP PGGLDARQAL PRAHPAGSFH AGPGDLQKWA QSQDGFEHKE FFSSQVGRKS 

       130        140        150        160        170        180 
QSAFYPEDDD YFFAAGQPGW HSHTQGTLGF PSPGEPGPRE GAFPAAQVQR RRVKKRHRRQ 

       190        200        210        220        230        240 
RRSHVLEEGD DGDRLYSSMS RAFLYRLWKG NVSSKMLNPR LQKAMKDYLT ANKHGVRFRG 

       250        260        270        280        290        300 
KREAGLSRAQ LLCQLRSRAR VRTLDGTEAP FSALGWRRLV PAVPLSQLHP RGLRSCAVVM 

       310        320        330        340        350        360 
SAGAILNSSL GEEIDSHDAV LRFNSAPTRG YEKDVGNKTT IRIINSQILT NPSHHFIDSS 

       370        380        390        400        410        420 
LYKDVILVAW DPAPYSANLN LWYKKPDYNL FTPYIQHRQR NPNQPFYILH PKFIWQLWDI 

       430        440        450        460        470        480 
IQENTKEKIQ PNPPSSGFIG ILIMMSMCRE VHVYEYIPSV RQTELCHYHE LYYDAACTLG 

       490        500        510        520 
AYHPLLYEKL LVQRLNMGTQ GDLHRKGKVV LPGFQAVHCP APSPVIPHS 

« Hide

Isoform 2 [UniParc].

Checksum: 9180AF089B7A181C
Show »

FASTA46652,927

References

« Hide 'large scale' references
[1]"Characterization of the second type of human beta-galactoside alpha2,6-sialyltransferase (ST6Gal II) that sialylates Galbeta1,4GlcNAc structures on oligosaccharides preferentially."
Takashima S., Tsuji S., Tsujimoto M.
J. Biol. Chem. 277:45719-45728(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[2]"Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.
DNA Res. 8:85-95(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Ovary.
[6]"Identification and functional expression of a second human beta-galactoside alpha2,6-sialyltransferase, ST6Gal II."
Krzewinski-Recchi M.-A., Julien S., Juliant S., Teintenier-Lelievre M., Samyn-Petit B., Montiel M.-D., Mir A.-M., Cerutti M., Harduin-Lepers A., Delannoy P.
Eur. J. Biochem. 270:950-961(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 315-529 (ISOFORM 1), ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[7]"Probing the substrate specificity of four different sialyltransferases using synthetic beta-D-Galp-(1-->4)-beta-D-GlcpNAc-(1-->2)-alpha-D-Manp-(1-->O) (CH(2))7CH3 analogues general activating effect of replacing N-acetylglucosamine by N-propionylglucosamine."
Rohfritsch P.F., Joosten J.A.F., Krzewinski-Recchi M.-A., Harduin-Lepers A., Laporte B., Juliant S., Cerutti M., Delannoy P., Vliegenthart J.F.G., Kamerling J.P.
Biochim. Biophys. Acta 1760:685-692(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME ACTIVITY.
[8]"IL-6 and IL-8 increase the expression of glycosyltransferases and sulfotransferases involved in the biosynthesis of sialylated and/or sulfated Lewis x epitopes in the human bronchial mucosa."
Groux-Degroote S., Krzewinski-Recchi M.-A., Cazet A., Vincent A., Lehoux S., Lafitte J.-J., van Seuningen I., Delannoy P.
Biochem. J. 410:213-223(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[9]"LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
Halim A., Ruetschi U., Larson G., Nilsson J.
J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT SER-69, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB059555 mRNA. Translation: BAC24793.1.
AB058780 mRNA. Translation: BAB47506.1. Different initiation.
AC016994 Genomic DNA. No translation available.
AC108049 Genomic DNA. Translation: AAY15022.1.
CH471127 Genomic DNA. Translation: EAX01742.1.
CH471127 Genomic DNA. Translation: EAX01743.1.
BC008680 mRNA. Translation: AAH08680.1. Different initiation.
AJ512141 mRNA. Translation: CAD54408.1.
RefSeqNP_001135823.1. NM_001142351.1.
NP_001135824.1. NM_001142352.1.
NP_115917.1. NM_032528.2.
UniGeneHs.98265.

3D structure databases

ProteinModelPortalQ96JF0.
SMRQ96JF0. Positions 205-519.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ96JF0. 1 interaction.
STRING9606.ENSP00000355273.

Protein family/group databases

CAZyGT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSiteQ96JF0.

Polymorphism databases

DMDM166219772.

Proteomic databases

PaxDbQ96JF0.
PRIDEQ96JF0.

Protocols and materials databases

DNASU84620.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361686; ENSP00000355273; ENSG00000144057. [Q96JF0-1]
ENST00000409087; ENSP00000387332; ENSG00000144057. [Q96JF0-2]
ENST00000409382; ENSP00000386942; ENSG00000144057. [Q96JF0-1]
GeneID84620.
KEGGhsa:84620.
UCSCuc002tdq.3. human. [Q96JF0-1]
uc002tds.3. human. [Q96JF0-2]

Organism-specific databases

CTD84620.
GeneCardsGC02M107418.
H-InvDBHIX0161642.
HGNCHGNC:10861. ST6GAL2.
HPAHPA014459.
MIM608472. gene.
neXtProtNX_Q96JF0.
PharmGKBPA35763.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG249416.
HOGENOMHOG000013206.
HOVERGENHBG052853.
InParanoidQ96JF0.
KOK00779.
OMAFTDSNPA.
PhylomeDBQ96JF0.
TreeFamTF323961.

Gene expression databases

ArrayExpressQ96JF0.
BgeeQ96JF0.
CleanExHS_ST6GAL2.
GenevestigatorQ96JF0.

Family and domain databases

InterProIPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR001675. Glyco_trans_29.
[Graphical view]
PfamPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
SUPFAMSSF88713. SSF88713. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi84620.
NextBio74504.
PROQ96JF0.
SOURCESearch...

Entry information

Entry nameSIAT2_HUMAN
AccessionPrimary (citable) accession number: Q96JF0
Secondary accession number(s): D3DVK3 expand/collapse secondary AC list , Q53QP4, Q86Y44, Q8IUG7, Q96HE4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: April 16, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM