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Q96JF0

- SIAT2_HUMAN

UniProt

Q96JF0 - SIAT2_HUMAN

Protein

Beta-galactoside alpha-2,6-sialyltransferase 2

Gene

ST6GAL2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. Has alpha-2,6-sialyltransferase activity toward oligosaccharides that have the Gal-beta-1,4-GlcNAc sequence at the non-reducing end of their carbohydrate groups, but it has weak or no activities toward glycoproteins and glycolipids.

    Catalytic activityi

    CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.3 Publications

    Kineticsi

    1. KM=0.74 mM for Gal-beta-1,4-GlcNAc-beta-O-octyl2 Publications
    2. KM=0.71 mM for Gal-beta-1,4-GlcNAc2 Publications
    3. KM=0.48 mM for lacto-N-neotetraose2 Publications

    GO - Molecular functioni

    1. beta-galactoside alpha-2,6-sialyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. growth Source: UniProtKB
    2. multicellular organismal development Source: UniProtKB
    3. oligosaccharide metabolic process Source: UniProtKB
    4. protein glycosylation Source: InterPro
    5. sialylation Source: GOC

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_200874. Sialic acid metabolism.

    Protein family/group databases

    CAZyiGT29. Glycosyltransferase Family 29.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactoside alpha-2,6-sialyltransferase 2 (EC:2.4.99.1)
    Short name:
    Alpha 2,6-ST 2
    Alternative name(s):
    CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 2
    ST6Gal II
    Short name:
    ST6GalII
    Short name:
    hST6Gal II
    Sialyltransferase 2
    Gene namesi
    Name:ST6GAL2
    Synonyms:KIAA1877, SIAT2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:10861. ST6GAL2.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi cisterna membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35763.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 529529Beta-galactoside alpha-2,6-sialyltransferase 2PRO_0000314785Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi69 – 691O-linked (GalNAc...)1 Publication
    Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi253 ↔ 519By similarity
    Disulfide bondi296 ↔ 448By similarity
    Disulfide bondi466 ↔ 477By similarity

    Post-translational modificationi

    O-glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ96JF0.
    PRIDEiQ96JF0.

    PTM databases

    PhosphoSiteiQ96JF0.

    Expressioni

    Tissue specificityi

    Weakly expressed in some tissues, such as small intestine, colon and fetal brain.2 Publications

    Inductioni

    By IL6/interleukin-6 and IL8//interleukin-8.1 Publication

    Gene expression databases

    ArrayExpressiQ96JF0.
    BgeeiQ96JF0.
    CleanExiHS_ST6GAL2.
    GenevestigatoriQ96JF0.

    Organism-specific databases

    HPAiHPA014459.

    Interactioni

    Protein-protein interaction databases

    IntActiQ96JF0. 1 interaction.
    STRINGi9606.ENSP00000355273.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96JF0.
    SMRiQ96JF0. Positions 205-519.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini33 – 529497LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 29 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG249416.
    HOGENOMiHOG000013206.
    HOVERGENiHBG052853.
    InParanoidiQ96JF0.
    KOiK00779.
    OMAiYFTDSNP.
    PhylomeDBiQ96JF0.
    TreeFamiTF323961.

    Family and domain databases

    InterProiIPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR001675. Glyco_trans_29.
    [Graphical view]
    PfamiPF00777. Glyco_transf_29. 1 hit.
    [Graphical view]
    SUPFAMiSSF88713. SSF88713. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q96JF0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKPHLKQWRQ RMLFGIFAWG LLFLLIFIYF TDSNPAEPVP SSLSFLETRR    50
    LLPVQGKQRA IMGAAHEPSP PGGLDARQAL PRAHPAGSFH AGPGDLQKWA 100
    QSQDGFEHKE FFSSQVGRKS QSAFYPEDDD YFFAAGQPGW HSHTQGTLGF 150
    PSPGEPGPRE GAFPAAQVQR RRVKKRHRRQ RRSHVLEEGD DGDRLYSSMS 200
    RAFLYRLWKG NVSSKMLNPR LQKAMKDYLT ANKHGVRFRG KREAGLSRAQ 250
    LLCQLRSRAR VRTLDGTEAP FSALGWRRLV PAVPLSQLHP RGLRSCAVVM 300
    SAGAILNSSL GEEIDSHDAV LRFNSAPTRG YEKDVGNKTT IRIINSQILT 350
    NPSHHFIDSS LYKDVILVAW DPAPYSANLN LWYKKPDYNL FTPYIQHRQR 400
    NPNQPFYILH PKFIWQLWDI IQENTKEKIQ PNPPSSGFIG ILIMMSMCRE 450
    VHVYEYIPSV RQTELCHYHE LYYDAACTLG AYHPLLYEKL LVQRLNMGTQ 500
    GDLHRKGKVV LPGFQAVHCP APSPVIPHS 529
    Length:529
    Mass (Da):60,158
    Last modified:January 15, 2008 - v2
    Checksum:iC3647E6DC0A9EFE3
    GO
    Isoform 2 (identifier: Q96JF0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         441-529: ILIMMSMCRE...PAPSPVIPHS → SFVKIGHIRACSEPRSRDCTPAWTTE

    Note: No experimental confirmation available.

    Show »
    Length:466
    Mass (Da):52,927
    Checksum:i9180AF089B7A181C
    GO

    Sequence cautioni

    The sequence AAH08680.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence BAB47506.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti154 – 1541G → R.
    Corresponds to variant rs3796110 [ dbSNP | Ensembl ].
    VAR_038046
    Natural varianti341 – 3411I → V.
    Corresponds to variant rs12615112 [ dbSNP | Ensembl ].
    VAR_038047

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei441 – 52989ILIMM…VIPHS → SFVKIGHIRACSEPRSRDCT PAWTTE in isoform 2. 1 PublicationVSP_030356Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB059555 mRNA. Translation: BAC24793.1.
    AB058780 mRNA. Translation: BAB47506.1. Different initiation.
    AC016994 Genomic DNA. No translation available.
    AC108049 Genomic DNA. Translation: AAY15022.1.
    CH471127 Genomic DNA. Translation: EAX01742.1.
    CH471127 Genomic DNA. Translation: EAX01743.1.
    BC008680 mRNA. Translation: AAH08680.1. Different initiation.
    AJ512141 mRNA. Translation: CAD54408.1.
    CCDSiCCDS2073.1. [Q96JF0-1]
    CCDS46380.1. [Q96JF0-2]
    RefSeqiNP_001135823.1. NM_001142351.1. [Q96JF0-1]
    NP_001135824.1. NM_001142352.1. [Q96JF0-2]
    NP_115917.1. NM_032528.2. [Q96JF0-1]
    XP_006712864.1. XM_006712801.1. [Q96JF0-1]
    XP_006712865.1. XM_006712802.1. [Q96JF0-1]
    UniGeneiHs.98265.

    Genome annotation databases

    EnsembliENST00000361686; ENSP00000355273; ENSG00000144057. [Q96JF0-1]
    ENST00000409087; ENSP00000387332; ENSG00000144057. [Q96JF0-2]
    ENST00000409382; ENSP00000386942; ENSG00000144057. [Q96JF0-1]
    GeneIDi84620.
    KEGGihsa:84620.
    UCSCiuc002tdq.3. human. [Q96JF0-1]
    uc002tds.3. human. [Q96JF0-2]

    Polymorphism databases

    DMDMi166219772.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    GGDB

    GlycoGene database

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB059555 mRNA. Translation: BAC24793.1 .
    AB058780 mRNA. Translation: BAB47506.1 . Different initiation.
    AC016994 Genomic DNA. No translation available.
    AC108049 Genomic DNA. Translation: AAY15022.1 .
    CH471127 Genomic DNA. Translation: EAX01742.1 .
    CH471127 Genomic DNA. Translation: EAX01743.1 .
    BC008680 mRNA. Translation: AAH08680.1 . Different initiation.
    AJ512141 mRNA. Translation: CAD54408.1 .
    CCDSi CCDS2073.1. [Q96JF0-1 ]
    CCDS46380.1. [Q96JF0-2 ]
    RefSeqi NP_001135823.1. NM_001142351.1. [Q96JF0-1 ]
    NP_001135824.1. NM_001142352.1. [Q96JF0-2 ]
    NP_115917.1. NM_032528.2. [Q96JF0-1 ]
    XP_006712864.1. XM_006712801.1. [Q96JF0-1 ]
    XP_006712865.1. XM_006712802.1. [Q96JF0-1 ]
    UniGenei Hs.98265.

    3D structure databases

    ProteinModelPortali Q96JF0.
    SMRi Q96JF0. Positions 205-519.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q96JF0. 1 interaction.
    STRINGi 9606.ENSP00000355273.

    Protein family/group databases

    CAZyi GT29. Glycosyltransferase Family 29.

    PTM databases

    PhosphoSitei Q96JF0.

    Polymorphism databases

    DMDMi 166219772.

    Proteomic databases

    PaxDbi Q96JF0.
    PRIDEi Q96JF0.

    Protocols and materials databases

    DNASUi 84620.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000361686 ; ENSP00000355273 ; ENSG00000144057 . [Q96JF0-1 ]
    ENST00000409087 ; ENSP00000387332 ; ENSG00000144057 . [Q96JF0-2 ]
    ENST00000409382 ; ENSP00000386942 ; ENSG00000144057 . [Q96JF0-1 ]
    GeneIDi 84620.
    KEGGi hsa:84620.
    UCSCi uc002tdq.3. human. [Q96JF0-1 ]
    uc002tds.3. human. [Q96JF0-2 ]

    Organism-specific databases

    CTDi 84620.
    GeneCardsi GC02M107418.
    H-InvDB HIX0161642.
    HGNCi HGNC:10861. ST6GAL2.
    HPAi HPA014459.
    MIMi 608472. gene.
    neXtProti NX_Q96JF0.
    PharmGKBi PA35763.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG249416.
    HOGENOMi HOG000013206.
    HOVERGENi HBG052853.
    InParanoidi Q96JF0.
    KOi K00779.
    OMAi YFTDSNP.
    PhylomeDBi Q96JF0.
    TreeFami TF323961.

    Enzyme and pathway databases

    Reactomei REACT_200874. Sialic acid metabolism.

    Miscellaneous databases

    GenomeRNAii 84620.
    NextBioi 74504.
    PROi Q96JF0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q96JF0.
    Bgeei Q96JF0.
    CleanExi HS_ST6GAL2.
    Genevestigatori Q96JF0.

    Family and domain databases

    InterProi IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR001675. Glyco_trans_29.
    [Graphical view ]
    Pfami PF00777. Glyco_transf_29. 1 hit.
    [Graphical view ]
    SUPFAMi SSF88713. SSF88713. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the second type of human beta-galactoside alpha2,6-sialyltransferase (ST6Gal II) that sialylates Galbeta1,4GlcNAc structures on oligosaccharides preferentially."
      Takashima S., Tsuji S., Tsujimoto M.
      J. Biol. Chem. 277:45719-45728(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    2. "Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.
      DNA Res. 8:85-95(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Ovary.
    6. "Identification and functional expression of a second human beta-galactoside alpha2,6-sialyltransferase, ST6Gal II."
      Krzewinski-Recchi M.-A., Julien S., Juliant S., Teintenier-Lelievre M., Samyn-Petit B., Montiel M.-D., Mir A.-M., Cerutti M., Harduin-Lepers A., Delannoy P.
      Eur. J. Biochem. 270:950-961(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 315-529 (ISOFORM 1), ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    7. "Probing the substrate specificity of four different sialyltransferases using synthetic beta-D-Galp-(1-->4)-beta-D-GlcpNAc-(1-->2)-alpha-D-Manp-(1-->O) (CH(2))7CH3 analogues general activating effect of replacing N-acetylglucosamine by N-propionylglucosamine."
      Rohfritsch P.F., Joosten J.A.F., Krzewinski-Recchi M.-A., Harduin-Lepers A., Laporte B., Juliant S., Cerutti M., Delannoy P., Vliegenthart J.F.G., Kamerling J.P.
      Biochim. Biophys. Acta 1760:685-692(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME ACTIVITY.
    8. "IL-6 and IL-8 increase the expression of glycosyltransferases and sulfotransferases involved in the biosynthesis of sialylated and/or sulfated Lewis x epitopes in the human bronchial mucosa."
      Groux-Degroote S., Krzewinski-Recchi M.-A., Cazet A., Vincent A., Lehoux S., Lafitte J.-J., van Seuningen I., Delannoy P.
      Biochem. J. 410:213-223(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    9. "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
      Halim A., Ruetschi U., Larson G., Nilsson J.
      J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT SER-69, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiSIAT2_HUMAN
    AccessioniPrimary (citable) accession number: Q96JF0
    Secondary accession number(s): D3DVK3
    , Q53QP4, Q86Y44, Q8IUG7, Q96HE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3