Q96JF0 (SIAT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactoside alpha-2,6-sialyltransferase 2
Short name=Alpha 2,6-ST 2
EC=2.4.99.1

Alternative name(s):
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 2
ST6Gal II
Short name=ST6GalII
Short name=hST6Gal II
Sialyltransferase 2

Gene names

Name:	ST6GAL2
Synonyms:	KIAA1877, SIAT2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. Has alpha-2,6-sialyltransferase activity toward oligosaccharides that have the Gal-beta-1,4-GlcNAc sequence at the non-reducing end of their carbohydrate groups, but it has weak or no activities toward glycoproteins and glycolipids.
Catalytic activity	CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine. Ref.1 Ref.6 Ref.7
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Weakly expressed in some tissues, such as small intestine, colon and fetal brain. Ref.1 Ref.6
Induction	By IL6/interleukin-6 and IL8//interleukin-8. Ref.8
Post-translational modification	O-glycosylated. Ref.9
Sequence similarities	Belongs to the glycosyltransferase 29 family.
Biophysicochemical properties	Kinetic parameters: KM=0.74 mM for Gal-beta-1,4-GlcNAc-beta-O-octyl Ref.1 Ref.6 KM=0.71 mM for Gal-beta-1,4-GlcNAc KM=0.48 mM for lacto-N-neotetraose
Sequence caution	The sequence AAH08680.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAB47506.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	growth Non-traceable author statement Ref.1. Source: UniProtKB multicellular organismal development Non-traceable author statement Ref.1. Source: UniProtKB oligosaccharide metabolic process Inferred from direct assay Ref.1. Source: UniProtKB protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Non-traceable author statement Ref.1. Source: UniProtKB
Molecular_function	beta-galactoside alpha-2,6-sialyltransferase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96JF0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96JF0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     441-529: ILIMMSMCRE...PAPSPVIPHS → SFVKIGHIRACSEPRSRDCTPAWTTE

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	Beta-galactoside alpha-2,6-sialyltransferase 2		PRO_0000314785
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	33 – 529	497	Lumenal Potential
Amino acid modifications
	Glycosylation	69	1	O-linked (GalNAc...) Ref.9
	Glycosylation	211	1	N-linked (GlcNAc...) Potential
	Disulfide bond	253 ↔ 519		By similarity
	Disulfide bond	296 ↔ 448		By similarity
	Disulfide bond	466 ↔ 477		By similarity
Natural variations
	Alternative sequence	441 – 529	89	ILIMM…VIPHS → SFVKIGHIRACSEPRSRDCT PAWTTE in isoform 2.		VSP_030356
	Natural variant	154	1	G → R. Corresponds to variant rs3796110 [ dbSNP | Ensembl ].		VAR_038046
	Natural variant	341	1	I → V. Corresponds to variant rs12615112 [ dbSNP | Ensembl ].		VAR_038047

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: C3647E6DC0A9EFE3	FASTA	529	60,158
	10 20 30 40 50 60 MKPHLKQWRQ RMLFGIFAWG LLFLLIFIYF TDSNPAEPVP SSLSFLETRR LLPVQGKQRA 70 80 90 100 110 120 IMGAAHEPSP PGGLDARQAL PRAHPAGSFH AGPGDLQKWA QSQDGFEHKE FFSSQVGRKS 130 140 150 160 170 180 QSAFYPEDDD YFFAAGQPGW HSHTQGTLGF PSPGEPGPRE GAFPAAQVQR RRVKKRHRRQ 190 200 210 220 230 240 RRSHVLEEGD DGDRLYSSMS RAFLYRLWKG NVSSKMLNPR LQKAMKDYLT ANKHGVRFRG 250 260 270 280 290 300 KREAGLSRAQ LLCQLRSRAR VRTLDGTEAP FSALGWRRLV PAVPLSQLHP RGLRSCAVVM 310 320 330 340 350 360 SAGAILNSSL GEEIDSHDAV LRFNSAPTRG YEKDVGNKTT IRIINSQILT NPSHHFIDSS 370 380 390 400 410 420 LYKDVILVAW DPAPYSANLN LWYKKPDYNL FTPYIQHRQR NPNQPFYILH PKFIWQLWDI 430 440 450 460 470 480 IQENTKEKIQ PNPPSSGFIG ILIMMSMCRE VHVYEYIPSV RQTELCHYHE LYYDAACTLG 490 500 510 520 AYHPLLYEKL LVQRLNMGTQ GDLHRKGKVV LPGFQAVHCP APSPVIPHS
	Isoform 2 [UniParc]. Checksum: 9180AF089B7A181C	FASTA	466	52,927
	10 20 30 40 50 60 MKPHLKQWRQ RMLFGIFAWG LLFLLIFIYF TDSNPAEPVP SSLSFLETRR LLPVQGKQRA 70 80 90 100 110 120 IMGAAHEPSP PGGLDARQAL PRAHPAGSFH AGPGDLQKWA QSQDGFEHKE FFSSQVGRKS 130 140 150 160 170 180 QSAFYPEDDD YFFAAGQPGW HSHTQGTLGF PSPGEPGPRE GAFPAAQVQR RRVKKRHRRQ 190 200 210 220 230 240 RRSHVLEEGD DGDRLYSSMS RAFLYRLWKG NVSSKMLNPR LQKAMKDYLT ANKHGVRFRG 250 260 270 280 290 300 KREAGLSRAQ LLCQLRSRAR VRTLDGTEAP FSALGWRRLV PAVPLSQLHP RGLRSCAVVM 310 320 330 340 350 360 SAGAILNSSL GEEIDSHDAV LRFNSAPTRG YEKDVGNKTT IRIINSQILT NPSHHFIDSS 370 380 390 400 410 420 LYKDVILVAW DPAPYSANLN LWYKKPDYNL FTPYIQHRQR NPNQPFYILH PKFIWQLWDI 430 440 450 460 IQENTKEKIQ PNPPSSGFIG SFVKIGHIRA CSEPRSRDCT PAWTTE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the second type of human beta-galactoside alpha2,6-sialyltransferase (ST6Gal II) that sialylates Galbeta1,4GlcNAc structures on oligosaccharides preferentially." Takashima S., Tsuji S., Tsujimoto M. J. Biol. Chem. 277:45719-45728(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[2]	"Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O. DNA Res. 8:85-95(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Ovary.
[6]	"Identification and functional expression of a second human beta-galactoside alpha2,6-sialyltransferase, ST6Gal II." Krzewinski-Recchi M.-A., Julien S., Juliant S., Teintenier-Lelievre M., Samyn-Petit B., Montiel M.-D., Mir A.-M., Cerutti M., Harduin-Lepers A., Delannoy P. Eur. J. Biochem. 270:950-961(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 315-529 (ISOFORM 1), ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[7]	"Probing the substrate specificity of four different sialyltransferases using synthetic beta-D-Galp-(1-->4)-beta-D-GlcpNAc-(1-->2)-alpha-D-Manp-(1-->O) (CH(2))7CH3 analogues general activating effect of replacing N-acetylglucosamine by N-propionylglucosamine." Rohfritsch P.F., Joosten J.A.F., Krzewinski-Recchi M.-A., Harduin-Lepers A., Laporte B., Juliant S., Cerutti M., Delannoy P., Vliegenthart J.F.G., Kamerling J.P. Biochim. Biophys. Acta 1760:685-692(2006) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYME ACTIVITY.
[8]	"IL-6 and IL-8 increase the expression of glycosyltransferases and sulfotransferases involved in the biosynthesis of sialylated and/or sulfated Lewis x epitopes in the human bronchial mucosa." Groux-Degroote S., Krzewinski-Recchi M.-A., Cazet A., Vincent A., Lehoux S., Lafitte J.-J., van Seuningen I., Delannoy P. Biochem. J. 410:213-223(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[9]	"LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins." Halim A., Ruetschi U., Larson G., Nilsson J. J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT SER-69, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB059555 mRNA. Translation: BAC24793.1. AB058780 mRNA. Translation: BAB47506.1. Different initiation. AC016994 Genomic DNA. No translation available. AC108049 Genomic DNA. Translation: AAY15022.1. CH471127 Genomic DNA. Translation: EAX01742.1. CH471127 Genomic DNA. Translation: EAX01743.1. BC008680 mRNA. Translation: AAH08680.1. Different initiation. AJ512141 mRNA. Translation: CAD54408.1.
IPI	IPI00063048. IPI00479942.
RefSeq	NP_001135823.1. NM_001142351.1. NP_001135824.1. NM_001142352.1. NP_115917.1. NM_032528.2.
UniGene	Hs.98265.
3D structure databases
ProteinModelPortal	Q96JF0.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000355273.
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
PTM databases
PhosphoSite	Q96JF0.
Polymorphism databases
DMDM	166219772.
Proteomic databases
PaxDb	Q96JF0.
PRIDE	Q96JF0.
Protocols and materials databases
DNASU	84620.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000361686; ENSP00000355273; ENSG00000144057. ENST00000409087; ENSP00000387332; ENSG00000144057. ENST00000409382; ENSP00000386942; ENSG00000144057.
GeneID	84620.
KEGG	hsa:84620.
UCSC	uc002tdq.3. human. uc002tds.3. human.
Organism-specific databases
CTD	84620.
GeneCards	GC02M107418.
H-InvDB	HIX0161642.
HGNC	HGNC:10861. ST6GAL2.
HPA	HPA014459.
MIM	608472. gene.
neXtProt	NX_Q96JF0.
PharmGKB	PA35763.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG249416.
HOGENOM	HOG000013206.
HOVERGEN	HBG052853.
InParanoid	Q96JF0.
KO	K00779.
OMA	FTDSNPA.
OrthoDB	EOG4V9TQT.
Gene expression databases
ArrayExpress	Q96JF0.
Bgee	Q96JF0.
CleanEx	HS_ST6GAL2.
Genevestigator	Q96JF0.
Family and domain databases
InterPro	IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR001675. Glyco_trans_29. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
SUPFAM	SSF88713. Glyco_hydro/deAcase_b/a-brl. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	84620.
NextBio	74504.
SOURCE	Search...

Entry information

Entry name

SIAT2_HUMAN

Accession

Primary (citable) accession number: Q96JF0
Secondary accession number(s): D3DVK3 , Q53QP4, Q86Y44, Q8IUG7, Q96HE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	May 29, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.