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Protein

Beta-galactoside alpha-2,6-sialyltransferase 2

Gene

ST6GAL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. Has alpha-2,6-sialyltransferase activity toward oligosaccharides that have the Gal-beta-1,4-GlcNAc sequence at the non-reducing end of their carbohydrate groups, but it has weak or no activities toward glycoproteins and glycolipids.

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.3 Publications

Kineticsi

  1. KM=0.74 mM for Gal-beta-1,4-GlcNAc-beta-O-octyl2 Publications
  2. KM=0.71 mM for Gal-beta-1,4-GlcNAc2 Publications
  3. KM=0.48 mM for lacto-N-neotetraose2 Publications

    GO - Molecular functioni

    • beta-galactoside alpha-2,6-sialyltransferase activity Source: UniProtKB

    GO - Biological processi

    • cellular protein metabolic process Source: Reactome
    • dolichol-linked oligosaccharide biosynthetic process Source: Reactome
    • growth Source: UniProtKB
    • multicellular organismal development Source: UniProtKB
    • oligosaccharide metabolic process Source: UniProtKB
    • post-translational protein modification Source: Reactome
    • protein N-linked glycosylation via asparagine Source: Reactome
    • sialylation Source: GOC
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.99.1. 2681.
    ReactomeiREACT_264366. Sialic acid metabolism.

    Protein family/group databases

    CAZyiGT29. Glycosyltransferase Family 29.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactoside alpha-2,6-sialyltransferase 2 (EC:2.4.99.1)
    Short name:
    Alpha 2,6-ST 2
    Alternative name(s):
    CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 2
    ST6Gal II
    Short name:
    ST6GalII
    Short name:
    hST6Gal II
    Sialyltransferase 2
    Gene namesi
    Name:ST6GAL2
    Synonyms:KIAA1877, SIAT2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640 Componenti: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:10861. ST6GAL2.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei12 – 3221Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST
    Topological domaini33 – 529497LumenalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    • Golgi cisterna membrane Source: UniProtKB-SubCell
    • Golgi membrane Source: Reactome
    • integral component of membrane Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35763.

    Polymorphism and mutation databases

    BioMutaiST6GAL2.
    DMDMi166219772.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 529529Beta-galactoside alpha-2,6-sialyltransferase 2PRO_0000314785Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi69 – 691O-linked (GalNAc...)1 Publication
    Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi253 ↔ 519By similarity
    Disulfide bondi296 ↔ 448By similarity
    Disulfide bondi466 ↔ 477By similarity

    Post-translational modificationi

    O-glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ96JF0.
    PRIDEiQ96JF0.

    PTM databases

    PhosphoSiteiQ96JF0.

    Expressioni

    Tissue specificityi

    Weakly expressed in some tissues, such as small intestine, colon and fetal brain.2 Publications

    Inductioni

    By IL6/interleukin-6 and IL8//interleukin-8.1 Publication

    Gene expression databases

    BgeeiQ96JF0.
    CleanExiHS_ST6GAL2.
    ExpressionAtlasiQ96JF0. baseline and differential.
    GenevisibleiQ96JF0. HS.

    Organism-specific databases

    HPAiHPA014459.

    Interactioni

    Protein-protein interaction databases

    IntActiQ96JF0. 1 interaction.
    STRINGi9606.ENSP00000355273.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96JF0.
    SMRiQ96JF0. Positions 205-519.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 29 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG249416.
    GeneTreeiENSGT00550000074444.
    HOGENOMiHOG000013206.
    HOVERGENiHBG052853.
    InParanoidiQ96JF0.
    KOiK00779.
    OMAiYFTDSNP.
    PhylomeDBiQ96JF0.
    TreeFamiTF323961.

    Family and domain databases

    InterProiIPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR001675. Glyco_trans_29.
    [Graphical view]
    PfamiPF00777. Glyco_transf_29. 1 hit.
    [Graphical view]
    SUPFAMiSSF88713. SSF88713. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q96JF0-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MKPHLKQWRQ RMLFGIFAWG LLFLLIFIYF TDSNPAEPVP SSLSFLETRR
    60 70 80 90 100
    LLPVQGKQRA IMGAAHEPSP PGGLDARQAL PRAHPAGSFH AGPGDLQKWA
    110 120 130 140 150
    QSQDGFEHKE FFSSQVGRKS QSAFYPEDDD YFFAAGQPGW HSHTQGTLGF
    160 170 180 190 200
    PSPGEPGPRE GAFPAAQVQR RRVKKRHRRQ RRSHVLEEGD DGDRLYSSMS
    210 220 230 240 250
    RAFLYRLWKG NVSSKMLNPR LQKAMKDYLT ANKHGVRFRG KREAGLSRAQ
    260 270 280 290 300
    LLCQLRSRAR VRTLDGTEAP FSALGWRRLV PAVPLSQLHP RGLRSCAVVM
    310 320 330 340 350
    SAGAILNSSL GEEIDSHDAV LRFNSAPTRG YEKDVGNKTT IRIINSQILT
    360 370 380 390 400
    NPSHHFIDSS LYKDVILVAW DPAPYSANLN LWYKKPDYNL FTPYIQHRQR
    410 420 430 440 450
    NPNQPFYILH PKFIWQLWDI IQENTKEKIQ PNPPSSGFIG ILIMMSMCRE
    460 470 480 490 500
    VHVYEYIPSV RQTELCHYHE LYYDAACTLG AYHPLLYEKL LVQRLNMGTQ
    510 520
    GDLHRKGKVV LPGFQAVHCP APSPVIPHS
    Length:529
    Mass (Da):60,158
    Last modified:January 15, 2008 - v2
    Checksum:iC3647E6DC0A9EFE3
    GO
    Isoform 2 (identifier: Q96JF0-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         441-529: ILIMMSMCRE...PAPSPVIPHS → SFVKIGHIRACSEPRSRDCTPAWTTE

    Note: No experimental confirmation available.
    Show »
    Length:466
    Mass (Da):52,927
    Checksum:i9180AF089B7A181C
    GO

    Sequence cautioni

    The sequence AAH08680.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
    The sequence BAB47506.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti154 – 1541G → R.
    Corresponds to variant rs3796110 [ dbSNP | Ensembl ].
    VAR_038046
    Natural varianti341 – 3411I → V.
    Corresponds to variant rs12615112 [ dbSNP | Ensembl ].
    VAR_038047

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei441 – 52989ILIMM…VIPHS → SFVKIGHIRACSEPRSRDCT PAWTTE in isoform 2. 1 PublicationVSP_030356Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB059555 mRNA. Translation: BAC24793.1.
    AB058780 mRNA. Translation: BAB47506.1. Different initiation.
    AC016994 Genomic DNA. No translation available.
    AC108049 Genomic DNA. Translation: AAY15022.1.
    CH471127 Genomic DNA. Translation: EAX01742.1.
    CH471127 Genomic DNA. Translation: EAX01743.1.
    BC008680 mRNA. Translation: AAH08680.1. Different initiation.
    AJ512141 mRNA. Translation: CAD54408.1.
    CCDSiCCDS2073.1. [Q96JF0-1]
    CCDS46380.1. [Q96JF0-2]
    RefSeqiNP_001135823.1. NM_001142351.1. [Q96JF0-1]
    NP_001135824.1. NM_001142352.1. [Q96JF0-2]
    NP_115917.1. NM_032528.2. [Q96JF0-1]
    XP_006712864.1. XM_006712801.2. [Q96JF0-1]
    XP_006712865.1. XM_006712802.1. [Q96JF0-1]
    UniGeneiHs.98265.

    Genome annotation databases

    EnsembliENST00000361686; ENSP00000355273; ENSG00000144057. [Q96JF0-1]
    ENST00000409087; ENSP00000387332; ENSG00000144057. [Q96JF0-2]
    ENST00000409382; ENSP00000386942; ENSG00000144057. [Q96JF0-1]
    GeneIDi84620.
    KEGGihsa:84620.
    UCSCiuc002tdq.3. human. [Q96JF0-1]
    uc002tds.3. human. [Q96JF0-2]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    GGDB

    GlycoGene database

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB059555 mRNA. Translation: BAC24793.1.
    AB058780 mRNA. Translation: BAB47506.1. Different initiation.
    AC016994 Genomic DNA. No translation available.
    AC108049 Genomic DNA. Translation: AAY15022.1.
    CH471127 Genomic DNA. Translation: EAX01742.1.
    CH471127 Genomic DNA. Translation: EAX01743.1.
    BC008680 mRNA. Translation: AAH08680.1. Different initiation.
    AJ512141 mRNA. Translation: CAD54408.1.
    CCDSiCCDS2073.1. [Q96JF0-1]
    CCDS46380.1. [Q96JF0-2]
    RefSeqiNP_001135823.1. NM_001142351.1. [Q96JF0-1]
    NP_001135824.1. NM_001142352.1. [Q96JF0-2]
    NP_115917.1. NM_032528.2. [Q96JF0-1]
    XP_006712864.1. XM_006712801.2. [Q96JF0-1]
    XP_006712865.1. XM_006712802.1. [Q96JF0-1]
    UniGeneiHs.98265.

    3D structure databases

    ProteinModelPortaliQ96JF0.
    SMRiQ96JF0. Positions 205-519.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiQ96JF0. 1 interaction.
    STRINGi9606.ENSP00000355273.

    Protein family/group databases

    CAZyiGT29. Glycosyltransferase Family 29.

    PTM databases

    PhosphoSiteiQ96JF0.

    Polymorphism and mutation databases

    BioMutaiST6GAL2.
    DMDMi166219772.

    Proteomic databases

    PaxDbiQ96JF0.
    PRIDEiQ96JF0.

    Protocols and materials databases

    DNASUi84620.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000361686; ENSP00000355273; ENSG00000144057. [Q96JF0-1]
    ENST00000409087; ENSP00000387332; ENSG00000144057. [Q96JF0-2]
    ENST00000409382; ENSP00000386942; ENSG00000144057. [Q96JF0-1]
    GeneIDi84620.
    KEGGihsa:84620.
    UCSCiuc002tdq.3. human. [Q96JF0-1]
    uc002tds.3. human. [Q96JF0-2]

    Organism-specific databases

    CTDi84620.
    GeneCardsiGC02M107418.
    H-InvDBHIX0161642.
    HGNCiHGNC:10861. ST6GAL2.
    HPAiHPA014459.
    MIMi608472. gene.
    neXtProtiNX_Q96JF0.
    PharmGKBiPA35763.
    HUGEiSearch...
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiNOG249416.
    GeneTreeiENSGT00550000074444.
    HOGENOMiHOG000013206.
    HOVERGENiHBG052853.
    InParanoidiQ96JF0.
    KOiK00779.
    OMAiYFTDSNP.
    PhylomeDBiQ96JF0.
    TreeFamiTF323961.

    Enzyme and pathway databases

    BRENDAi2.4.99.1. 2681.
    ReactomeiREACT_264366. Sialic acid metabolism.

    Miscellaneous databases

    ChiTaRSiST6GAL2. human.
    GenomeRNAii84620.
    NextBioi74504.
    PROiQ96JF0.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ96JF0.
    CleanExiHS_ST6GAL2.
    ExpressionAtlasiQ96JF0. baseline and differential.
    GenevisibleiQ96JF0. HS.

    Family and domain databases

    InterProiIPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR001675. Glyco_trans_29.
    [Graphical view]
    PfamiPF00777. Glyco_transf_29. 1 hit.
    [Graphical view]
    SUPFAMiSSF88713. SSF88713. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Characterization of the second type of human beta-galactoside alpha2,6-sialyltransferase (ST6Gal II) that sialylates Galbeta1,4GlcNAc structures on oligosaccharides preferentially."
      Takashima S., Tsuji S., Tsujimoto M.
      J. Biol. Chem. 277:45719-45728(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    2. "Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.
      DNA Res. 8:85-95(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Ovary.
    6. "Identification and functional expression of a second human beta-galactoside alpha2,6-sialyltransferase, ST6Gal II."
      Krzewinski-Recchi M.-A., Julien S., Juliant S., Teintenier-Lelievre M., Samyn-Petit B., Montiel M.-D., Mir A.-M., Cerutti M., Harduin-Lepers A., Delannoy P.
      Eur. J. Biochem. 270:950-961(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 315-529 (ISOFORM 1), ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    7. "Probing the substrate specificity of four different sialyltransferases using synthetic beta-D-Galp-(1-->4)-beta-D-GlcpNAc-(1-->2)-alpha-D-Manp-(1-->O) (CH(2))7CH3 analogues general activating effect of replacing N-acetylglucosamine by N-propionylglucosamine."
      Rohfritsch P.F., Joosten J.A.F., Krzewinski-Recchi M.-A., Harduin-Lepers A., Laporte B., Juliant S., Cerutti M., Delannoy P., Vliegenthart J.F.G., Kamerling J.P.
      Biochim. Biophys. Acta 1760:685-692(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME ACTIVITY.
    8. "IL-6 and IL-8 increase the expression of glycosyltransferases and sulfotransferases involved in the biosynthesis of sialylated and/or sulfated Lewis x epitopes in the human bronchial mucosa."
      Groux-Degroote S., Krzewinski-Recchi M.-A., Cazet A., Vincent A., Lehoux S., Lafitte J.-J., van Seuningen I., Delannoy P.
      Biochem. J. 410:213-223(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    9. "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
      Halim A., Ruetschi U., Larson G., Nilsson J.
      J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT SER-69, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiSIAT2_HUMAN
    AccessioniPrimary (citable) accession number: Q96JF0
    Secondary accession number(s): D3DVK3
    , Q53QP4, Q86Y44, Q8IUG7, Q96HE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 15, 2008
    Last modified: June 24, 2015
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.