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Protein

1,5-anhydro-D-fructose reductase

Gene

AKR1E2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of 1,5-anhydro-D-fructose (AF) to 1,5-anhydro-D-glucitol. Can also catalyze the reduction of various aldehydes and quinones (By similarity). Has low NADPH-dependent reductase activity towards 9,10-phenanthrenequinone (in vitro).By similarity

Catalytic activityi

1,5-anhydro-D-glucitol + NADP+ = 1,5-anhydro-D-fructose + NADPH.

Kineticsi

  1. KM=6.1 µM for 9,10-phenanthrenequinone with NADPH as cofactor1 Publication
  1. Vmax=0.42 nmol/min/mg enzyme towards 9,10-phenanthrenequinone with NADPH as cofactor1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35NADPBy similarity1
Active sitei40Proton donorBy similarity1
Sitei69Lowers pKa of active site TyrBy similarity1
Binding sitei102SubstrateBy similarity1
Binding sitei193NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi269 – 277NADPBy similarity9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:HS15341-MONOMER.
ZFISH:HS15341-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
1,5-anhydro-D-fructose reductase (EC:1.1.1.263)
Short name:
AF reductase
Alternative name(s):
Aldo-keto reductase family 1 member C-like protein 2
Aldo-keto reductase family 1 member E2
LoopADR
Testis-specific protein
Short name:
hTSP
Gene namesi
Name:AKR1E2
Synonyms:AKR1CL2, AKRDC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:23437. AKR1E2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi83592.
OpenTargetsiENSG00000165568.
PharmGKBiPA165548224.

Polymorphism and mutation databases

DMDMi269849539.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002878801 – 3201,5-anhydro-D-fructose reductaseAdd BLAST320

Proteomic databases

PaxDbiQ96JD6.
PeptideAtlasiQ96JD6.
PRIDEiQ96JD6.

PTM databases

iPTMnetiQ96JD6.
PhosphoSitePlusiQ96JD6.

Expressioni

Tissue specificityi

Testis-specific.1 Publication

Gene expression databases

BgeeiENSG00000165568.
CleanExiHS_AKR1CL2.
ExpressionAtlasiQ96JD6. baseline and differential.
GenevisibleiQ96JD6. HS.

Organism-specific databases

HPAiHPA037821.
HPA037822.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000298375.

Structurei

3D structure databases

ProteinModelPortaliQ96JD6.
SMRiQ96JD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiQ96JD6.
KOiK13981.
OMAiGAGIRCK.
OrthoDBiEOG091G0D69.
PhylomeDBiQ96JD6.
TreeFamiTF106492.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96JD6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDIPAVGLS SWKASPGKVT EAVKEAIDAG YRHFDCAYFY HNEREVGAGI
60 70 80 90 100
RCKIKEGAVR REDLFIATKL WCTCHKKSLV ETACRKSLKA LKLNYLDLYL
110 120 130 140 150
IHWPMGFKPP HPEWIMSCSE LSFCLSHPRV QDLPLDESNM VIPSDTDFLD
160 170 180 190 200
TWEAMEDLVI TGLVKNIGVS NFNHEQLERL LNKPGLRFKP LTNQIECHPY
210 220 230 240 250
LTQKNLISFC QSRDVSVTAY RPLGGSCEGV DLIDNPVIKR IAKEHGKSPA
260 270 280 290 300
QILIRFQIQR NVIVIPGSIT PSHIKENIQV FDFELTQHDM DNILSLNRNL
310 320
RLAMFPITKN HKDYPFHIEY
Length:320
Mass (Da):36,589
Last modified:November 24, 2009 - v2
Checksum:iA73E06225E7F15DD
GO
Isoform 2 (identifier: Q96JD6-2) [UniParc]FASTAAdd to basket
Also known as: HTSP2

The sequence of this isoform differs from the canonical sequence as follows:
     194-250: Missing.

Show »
Length:263
Mass (Da):30,323
Checksum:i024E146FABDEBA9F
GO
Isoform 3 (identifier: Q96JD6-3) [UniParc]FASTAAdd to basket
Also known as: HTSP1

The sequence of this isoform differs from the canonical sequence as follows:
     194-250: Missing.
     307-307: I → M
     308-320: Missing.

Show »
Length:250
Mass (Da):28,667
Checksum:i241A3E8125FDE258
GO
Isoform 4 (identifier: Q96JD6-4) [UniParc]FASTAAdd to basket
Also known as: HTSP4

The sequence of this isoform differs from the canonical sequence as follows:
     307-307: I → M
     308-320: Missing.

Show »
Length:307
Mass (Da):34,933
Checksum:i4DEEDE07568D2F03
GO
Isoform 5 (identifier: Q96JD6-5) [UniParc]FASTAAdd to basket
Also known as: HTSP3

The sequence of this isoform differs from the canonical sequence as follows:
     154-251: Missing.

Show »
Length:222
Mass (Da):25,693
Checksum:i388A3D53CEA44BE4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti312K → N in AAK58523 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03235652C → G.Corresponds to variant rs35429729dbSNPEnsembl.1
Natural variantiVAR_03235786K → R.Corresponds to variant rs17133693dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025614154 – 251Missing in isoform 5. 1 PublicationAdd BLAST98
Alternative sequenceiVSP_025615194 – 250Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST57
Alternative sequenceiVSP_025616307I → M in isoform 3 and isoform 4. 2 Publications1
Alternative sequenceiVSP_025617308 – 320Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040820 mRNA. Translation: BAC54565.1.
AB040821 mRNA. Translation: BAC54566.1.
AB040822 mRNA. Translation: BAC54567.1.
AB055603 mRNA. Translation: BAC54568.1.
AF263242 mRNA. Translation: AAK58523.1.
AC091817 Genomic DNA. No translation available.
BC002862 mRNA. Translation: AAH02862.1.
CCDSiCCDS31134.1. [Q96JD6-1]
CCDS59209.1. [Q96JD6-2]
CCDS59210.1. [Q96JD6-5]
RefSeqiNP_001035267.1. NM_001040177.2. [Q96JD6-1]
NP_001257950.1. NM_001271021.1. [Q96JD6-2]
NP_001257954.1. NM_001271025.1. [Q96JD6-5]
UniGeneiHs.657944.

Genome annotation databases

EnsembliENST00000298375; ENSP00000298375; ENSG00000165568. [Q96JD6-1]
ENST00000334019; ENSP00000335034; ENSG00000165568. [Q96JD6-2]
ENST00000345253; ENSP00000335603; ENSG00000165568. [Q96JD6-5]
ENST00000463345; ENSP00000436794; ENSG00000165568. [Q96JD6-4]
ENST00000532248; ENSP00000432947; ENSG00000165568. [Q96JD6-3]
GeneIDi83592.
KEGGihsa:83592.
UCSCiuc001ihi.5. human. [Q96JD6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040820 mRNA. Translation: BAC54565.1.
AB040821 mRNA. Translation: BAC54566.1.
AB040822 mRNA. Translation: BAC54567.1.
AB055603 mRNA. Translation: BAC54568.1.
AF263242 mRNA. Translation: AAK58523.1.
AC091817 Genomic DNA. No translation available.
BC002862 mRNA. Translation: AAH02862.1.
CCDSiCCDS31134.1. [Q96JD6-1]
CCDS59209.1. [Q96JD6-2]
CCDS59210.1. [Q96JD6-5]
RefSeqiNP_001035267.1. NM_001040177.2. [Q96JD6-1]
NP_001257950.1. NM_001271021.1. [Q96JD6-2]
NP_001257954.1. NM_001271025.1. [Q96JD6-5]
UniGeneiHs.657944.

3D structure databases

ProteinModelPortaliQ96JD6.
SMRiQ96JD6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000298375.

PTM databases

iPTMnetiQ96JD6.
PhosphoSitePlusiQ96JD6.

Polymorphism and mutation databases

DMDMi269849539.

Proteomic databases

PaxDbiQ96JD6.
PeptideAtlasiQ96JD6.
PRIDEiQ96JD6.

Protocols and materials databases

DNASUi83592.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000298375; ENSP00000298375; ENSG00000165568. [Q96JD6-1]
ENST00000334019; ENSP00000335034; ENSG00000165568. [Q96JD6-2]
ENST00000345253; ENSP00000335603; ENSG00000165568. [Q96JD6-5]
ENST00000463345; ENSP00000436794; ENSG00000165568. [Q96JD6-4]
ENST00000532248; ENSP00000432947; ENSG00000165568. [Q96JD6-3]
GeneIDi83592.
KEGGihsa:83592.
UCSCiuc001ihi.5. human. [Q96JD6-1]

Organism-specific databases

CTDi83592.
DisGeNETi83592.
GeneCardsiAKR1E2.
H-InvDBHIX0008606.
HGNCiHGNC:23437. AKR1E2.
HPAiHPA037821.
HPA037822.
neXtProtiNX_Q96JD6.
OpenTargetsiENSG00000165568.
PharmGKBiPA165548224.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiQ96JD6.
KOiK13981.
OMAiGAGIRCK.
OrthoDBiEOG091G0D69.
PhylomeDBiQ96JD6.
TreeFamiTF106492.

Enzyme and pathway databases

BioCyciMetaCyc:HS15341-MONOMER.
ZFISH:HS15341-MONOMER.

Miscellaneous databases

GenomeRNAii83592.
PROiQ96JD6.

Gene expression databases

BgeeiENSG00000165568.
CleanExiHS_AKR1CL2.
ExpressionAtlasiQ96JD6. baseline and differential.
GenevisibleiQ96JD6. HS.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKCL2_HUMAN
AccessioniPrimary (citable) accession number: Q96JD6
Secondary accession number(s): Q86Z16
, Q86Z17, Q86Z18, Q9BU71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: November 24, 2009
Last modified: November 2, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.