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Protein

ELL-associated factor 1

Gene

EAF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional transactivator of ELL and ELL2 elongation activities.2 Publications

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-75955 RNA Polymerase II Transcription Elongation
SIGNORiQ96JC9

Names & Taxonomyi

Protein namesi
Recommended name:
ELL-associated factor 1
Gene namesi
Name:EAF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000144597.13
HGNCiHGNC:20907 EAF1
MIMi608315 gene
neXtProtiNX_Q96JC9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi85403
OpenTargetsiENSG00000144597
PharmGKBiPA134897202

Polymorphism and mutation databases

BioMutaiEAF1
DMDMi73919265

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001303341 – 268ELL-associated factor 1Add BLAST268

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei165PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ96JC9
MaxQBiQ96JC9
PaxDbiQ96JC9
PeptideAtlasiQ96JC9
PRIDEiQ96JC9

PTM databases

iPTMnetiQ96JC9
PhosphoSitePlusiQ96JC9

Expressioni

Tissue specificityi

Strongly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine and colon. Poorly expressed in thymus.1 Publication

Gene expression databases

BgeeiENSG00000144597
CleanExiHS_EAF1
ExpressionAtlasiQ96JC9 baseline and differential
GenevisibleiQ96JC9 HS

Organism-specific databases

HPAiHPA059516
HPA060569
HPA069538

Interactioni

Subunit structurei

Component of the super elongation complex (SEC), at least composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb complex and ELL (ELL, ELL2 or ELL3). Interacts with ELL and ELL2.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi124514, 75 interactors
CORUMiQ96JC9
IntActiQ96JC9, 46 interactors
MINTiQ96JC9
STRINGi9606.ENSP00000380054

Structurei

3D structure databases

ProteinModelPortaliQ96JC9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni182 – 262Necessary for transactivation activityAdd BLAST81

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi127 – 168Pro-richAdd BLAST42
Compositional biasi188 – 211Ser-richAdd BLAST24

Sequence similaritiesi

Belongs to the EAF family.Curated

Phylogenomic databases

eggNOGiKOG4795 Eukaryota
ENOG4111KQJ LUCA
GeneTreeiENSGT00390000017724
HOGENOMiHOG000070137
HOVERGENiHBG054898
InParanoidiQ96JC9
KOiK15186
OMAiSCNIQVK
OrthoDBiEOG091G0MMP
PhylomeDBiQ96JC9
TreeFamiTF320864

Family and domain databases

InterProiView protein in InterPro
IPR027093 EAF_fam
IPR019194 Tscrpt_elong_fac_Eaf_N
PANTHERiPTHR15970 PTHR15970, 1 hit
PfamiView protein in Pfam
PF09816 EAF, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96JC9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNGTANPLLD REEHCLRLGE SFEKRPRASF HTIRYDFKPA SIDTSCEGEL
60 70 80 90 100
QVGKGDEVTI TLPHIPGSTP PMTVFKGNKR PYQKDCVLII NHDTGEYVLE
110 120 130 140 150
KLSSSIQVKK TRAEGSSKIQ ARMEQQPTRP PQTSQPPPPP PPMPFRAPTK
160 170 180 190 200
PPVGPKTSPL KDNPSPEPQL DDIKRELRAE VDIIEQMSSS SGSSSSDSES
210 220 230 240 250
SSGSDDDSSS SGGEDNGPAS PPQPSHQQPY NSRPAVANGT SRPQGSNQLM
260
NTLRNDLQLS ESGSDSDD
Length:268
Mass (Da):29,042
Last modified:December 1, 2001 - v1
Checksum:i0D78B586AABB0FA4
GO
Isoform 2 (identifier: Q96JC9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: MNGTANPLLDR → MKSQLHCHISL
     12-112: Missing.

Note: No experimental confirmation available.
Show »
Length:167
Mass (Da):17,723
Checksum:i997058BFE04D90E1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti97Y → F in AAH41329 (PubMed:15489334).Curated1
Sequence conflicti252T → A in AAH41329 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0561931 – 11MNGTANPLLDR → MKSQLHCHISL in isoform 2. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_05619412 – 112Missing in isoform 2. 1 PublicationAdd BLAST101

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF272973 mRNA Translation: AAK58687.1
AK304697 mRNA Translation: BAG65467.1
AC027125 Genomic DNA No translation available.
BC041329 mRNA Translation: AAH41329.1
CCDSiCCDS2626.1 [Q96JC9-1]
RefSeqiNP_149074.3, NM_033083.6 [Q96JC9-1]
XP_005265575.1, XM_005265518.1 [Q96JC9-2]
XP_011532467.1, XM_011534165.1 [Q96JC9-2]
XP_011532468.1, XM_011534166.1 [Q96JC9-2]
UniGeneiHs.716733

Genome annotation databases

EnsembliENST00000396842; ENSP00000380054; ENSG00000144597 [Q96JC9-1]
GeneIDi85403
KEGGihsa:85403
UCSCiuc003bzu.4 human [Q96JC9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiEAF1_HUMAN
AccessioniPrimary (citable) accession number: Q96JC9
Secondary accession number(s): B4E3F5, Q8IW10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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