Q96JB5 (CK5P3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CDK5 regulatory subunit-associated protein 3

Alternative name(s):
CDK5 activator-binding protein C53
Protein HSF-27

Gene names

Name:	CDK5RAP3
Synonyms:	IC53
ORF Names:	MSTP016, OK/SW-cl.114, PP1553

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1 By similarity. Ref.2
Subunit structure	Interacts with CDK5R1. This interaction is prevented by the association between CDK5R1 and CDK5RAP3 By similarity. Interacts with the enteric adenovirus serotype 41 fiber protein. Interacts with UFL1. Ref.10
Tissue specificity	Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta. Ref.1 Ref.2 Ref.9
Post-translational modification	Phosphorylated in vitro by CDK5 By similarity.
Sequence similarities	Belongs to the CDK5RAP3 family.
Sequence caution	The sequence AAK69655.1 differs from that shown. Reason: Frameshift at position 410.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	brain development Non-traceable author statement PubMed 10915792. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB regulation of neuron differentiation Non-traceable author statement Ref.9. Source: UniProtKB
Molecular_function	protein kinase binding Non-traceable author statement Ref.9. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RELA	Q04206	4	EBI-718818,EBI-73886

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96JB5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96JB5-2)

Also known as: IC53;

The sequence of this isoform differs from the canonical sequence as follows:
     1-87: Missing.
     88-111: RYSSQRMKDWQEIIALYEKDNTYL → MCVHPGACLPHVGVSWAEFPGHFS

Note: Due to an intron retention.

Isoform 3 (identifier: Q96JB5-3)

Also known as: IC53-2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-225: Missing.

Note: Due to intron retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	CDK5 regulatory subunit-associated protein 3		PRO_0000220516
Natural variations
	Alternative sequence	1 – 225	225	Missing in isoform 3.		VSP_007568
	Alternative sequence	1 – 87	87	Missing in isoform 2.		VSP_007566
	Alternative sequence	88 – 111	24	RYSSQ…DNTYL → MCVHPGACLPHVGVSWAEFP GHFS in isoform 2.		VSP_007567
	Natural variant	324	1	L → V. Corresponds to variant rs35054799 [ dbSNP | Ensembl ].		VAR_048688
Experimental info
	Sequence conflict	113	1	E → V in BAD97107. Ref.6
	Sequence conflict	406	1	E → K in AAK69655. Ref.3
	Sequence conflict	443	1	Q → E in BAD97107. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 16, 2003. Version 2. Checksum: A21E59A79E1F87E0	FASTA	506	56,921
	10 20 30 40 50 60 MEDHQHVPID IQTSKLLDWL VDRRHCSLKW QSLVLTIREK INAAIQDMPE SEEIAQLLSG 70 80 90 100 110 120 SYIHYFHCLR ILDLLKGTEA STKNIFGRYS SQRMKDWQEI IALYEKDNTY LVELSSLLVR 130 140 150 160 170 180 NVNYEIPSLK KQIAKCQQLQ QEYSRKEEEC QAGAAEMREQ FYHSCKQYGI TGENVRGELL 190 200 210 220 230 240 ALVKDLPSQL AEIGAAAQQS LGEAIDVYQA SVGFVCESPT EQVLPMLRFV QKRGNSTVYE 250 260 270 280 290 300 WRTGTEPSVV ERPHLEELPE QVAEDAIDWG DFGVEAVSEG TDSGISAEAA GIDWGIFPES 310 320 330 340 350 360 DSKDPGGDGI DWGDDAVALQ ITVLEAGTQA PEGVARGPDA LTLLEYTETR NQFLDELMEL 370 380 390 400 410 420 EIFLAQRAVE LSEEADVLSV SQFQLAPAIL QGQTKEKMVT MVSVLEDLIG KLTSLQLQHL 430 440 450 460 470 480 FMILASPRYV DRVTEFLQQK LKQSQLLALK KELMVQKQQE ALEEQAALEP KLDLLLEKTK 490 500 ELQKLIEADI SKRYSGRPVN LMGTSL
	Isoform 2 (IC53) [UniParc]. Checksum: DA15DED087A5A795	FASTA	419	46,351
	10 20 30 40 50 60 MCVHPGACLP HVGVSWAEFP GHFSVELSSL LVRNVNYEIP SLKKQIAKCQ QLQQEYSRKE 70 80 90 100 110 120 EECQAGAAEM REQFYHSCKQ YGITGENVRG ELLALVKDLP SQLAEIGAAA QQSLGEAIDV 130 140 150 160 170 180 YQASVGFVCE SPTEQVLPML RFVQKRGNST VYEWRTGTEP SVVERPHLEE LPEQVAEDAI 190 200 210 220 230 240 DWGDFGVEAV SEGTDSGISA EAAGIDWGIF PESDSKDPGG DGIDWGDDAV ALQITVLEAG 250 260 270 280 290 300 TQAPEGVARG PDALTLLEYT ETRNQFLDEL MELEIFLAQR AVELSEEADV LSVSQFQLAP 310 320 330 340 350 360 AILQGQTKEK MVTMVSVLED LIGKLTSLQL QHLFMILASP RYVDRVTEFL QQKLKQSQLL 370 380 390 400 410 ALKKELMVQK QQEALEEQAA LEPKLDLLLE KTKELQKLIE ADISKRYSGR PVNLMGTSL
	Isoform 3 (IC53-2) [UniParc]. Checksum: BDC6C94A65DF078C	FASTA	281	31,200
	10 20 30 40 50 60 MLRFVQKRGN STVYEWRTGT EPSVVERPHL EELPEQVAED AIDWGDFGVE AVSEGTDSGI 70 80 90 100 110 120 SAEAAGIDWG IFPESDSKDP GGDGIDWGDD AVALQITVLE AGTQAPEGVA RGPDALTLLE 130 140 150 160 170 180 YTETRNQFLD ELMELEIFLA QRAVELSEEA DVLSVSQFQL APAILQGQTK EKMVTMVSVL 190 200 210 220 230 240 EDLIGKLTSL QLQHLFMILA SPRYVDRVTE FLQQKLKQSQ LLALKKELMV QKQQEALEEQ 250 260 270 280 AALEPKLDLL LEKTKELQKL IEADISKRYS GRPVNLMGTS L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel gene IC53 stimulates ECV304 cell proliferation and is upregulated in failing heart." Chen J., Liu B., Liu Y.Q., Han Y., Yu H., Zhang Y., Lu L., Zhen Y., Hui R.T. Biochem. Biophys. Res. Commun. 294:161-166(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Aorta.
[2]	"Cloning and characterization of human IC53-2, a novel CDK5 activator binding protein." Xie Y.H., He X.H., Tang Y.T., Li J.J., Pan Z.M., Qin W.X., Wan da F., Gu J.R. Cell Res. 13:83-91(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, TISSUE SPECIFICITY. Tissue: Placenta.
[3]	"Protein interacting with the receptor binding domain of enteric adenovirus serotype 41 fiber protein." Favier A.-L., Harsi C., Chrobozcek J. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Kidney.
[4]	"Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients." Shichijo S., Itoh K. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon adenocarcinoma.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[6]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Synovial cell.
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[9]	"Cloning of three novel neuronal Cdk5 activator binding proteins." Ching Y.-P., Qi Z., Wang J.H. Gene 242:285-294(2000) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[10]	"A novel LZAP-binding protein, NLBP, inhibits cell invasion." Kwon J., Cho H.J., Han S.H., No J.G., Kwon J.Y., Kim H. J. Biol. Chem. 285:12232-12240(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UFL1.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF110322 mRNA. Translation: AAG39277.1. AF217982 mRNA. Translation: AAG17225.1. AF343090 mRNA. Translation: AAK69655.1. Frameshift. AB062433 mRNA. Translation: BAB93496.1. AK023722 mRNA. Translation: BAB14657.1. AK223387 mRNA. Translation: BAD97107.1. CH471109 Genomic DNA. Translation: EAW94772.1. CH471109 Genomic DNA. Translation: EAW94774.1. CH471109 Genomic DNA. Translation: EAW94775.1. CH471109 Genomic DNA. Translation: EAW94778.1. BC009957 mRNA. Translation: AAH09957.1. BC072435 mRNA. Translation: AAH72435.1.
IPI	IPI00018780. IPI00187145. IPI00414442.
PIR	JC7863.
RefSeq	NP_788276.1. NM_176096.1.
UniGene	Hs.20157.
3D structure databases
ProteinModelPortal	Q96JB5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96JB5. 15 interactions.
MINT	MINT-1410069.
STRING	9606.ENSP00000344683.
PTM databases
PhosphoSite	Q96JB5.
Polymorphism databases
DMDM	32129444.
Proteomic databases
PaxDb	Q96JB5.
PRIDE	Q96JB5.
Protocols and materials databases
DNASU	80279.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000338399; ENSP00000344683; ENSG00000108465.
GeneID	80279.
KEGG	hsa:80279.
UCSC	uc002imq.1. human. uc002ims.3. human.
Organism-specific databases
CTD	80279.
GeneCards	GC17P046048.
H-InvDB	HIX0013932.
HGNC	HGNC:18673. CDK5RAP3.
HPA	HPA022141. HPA022882. HPA027883.
MIM	608202. gene.
neXtProt	NX_Q96JB5.
PharmGKB	PA38633.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG237881.
HOVERGEN	HBG050978.
InParanoid	Q96JB5.
OrthoDB	EOG4BZN2K.
Gene expression databases
ArrayExpress	Q96JB5.
Bgee	Q96JB5.
CleanEx	HS_CDK5RAP3.
Genevestigator	Q96JB5.
GermOnline	ENSG00000108465. Homo sapiens.
Family and domain databases
InterPro	IPR008491. DUF773. [Graphical view]
PANTHER	PTHR14894. PTHR14894. 1 hit.
Pfam	PF05600. DUF773. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CDK5RAP3. human.
GenomeRNAi	80279.
NextBio	70750.
SOURCE	Search...

Entry information

Entry name

CK5P3_HUMAN

Accession

Primary (citable) accession number: Q96JB5
Secondary accession number(s): D3DTU1 , D3DTU2, Q53FA2, Q9H3F8, Q9H8G0, Q9HBR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 16, 2003
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.