CDK5 regulatory subunit-associated protein 3

Names and origin

Protein names

Recommended name:
    CDK5 regulatory subunit-associated protein 3
Alternative name(s):
    CDK5 activator-binding protein C53
    Protein HSF-27

Gene names

Name:	CDK5RAP3
Synonyms:	IC53
ORF Names:	MSTP016, OK/SW-cl.114, PP1553

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1 By similarity.
Subunit structure	Interacts with CDK5R1. This interaction is prevented by the association between CDK5R1 and CDK5RAP3 By similarity. Interacts with the enteric adenovirus serotype 41 fiber protein.
Tissue specificity	Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta. Ref.1 Ref.2 Ref.8
Post-translational modification	Phosphorylated in vitro by CDK5 By similarity.
Sequence similarities	Belongs to the CDK5RAP3 family.
Sequence caution	The sequence AAK69655.1 differs from that shown. Reason: Frameshift at position 410.

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Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	brain development Non-traceable author statement. Source: UniProtKB regulation of cyclin-dependent protein kinase activity Inferred from sequence or structural similarity. Source: UniProtKB regulation of neuron differentiation Ref.8 Non-traceable author statement. Source: UniProtKB
Molecular function	neuronal Cdc2-like kinase binding Ref.8 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
ARL1	P40616	1	EBI-718818,EBI-1052746
DDRGK1	Q96HY6	1	EBI-718818,EBI-1054024
DISC1	Q9NRI5	2	EBI-718818,EBI-529989
IGHA1	P01876	1	EBI-718818,EBI-727651
IGKC	P01834	1	EBI-718818,EBI-1054530
KHK	P50053	1	EBI-718818,EBI-1053974
KIAA0776	O94874	1	EBI-718818,EBI-1048088
RELA	Q04206	3	EBI-718818,EBI-73886
SMNDC1	O75940	1	EBI-718818,EBI-1052641
UFC1	Q9Y3C8	1	EBI-718818,EBI-1045733
UFM1	P61960	1	EBI-718818,EBI-1045061

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96JB5-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96JB5-2) Also known as: IC53; The sequence of this isoform differs from the canonical sequence as follows: 1-87: Missing. 88-111: RYSSQRMKDWQEIIALYEKDNTYL → MCVHPGACLPHVGVSWAEFPGHFS
Note: Due to an intron retention.

Isoform 3 (identifier: Q96JB5-3) Also known as: IC53-2; The sequence of this isoform differs from the canonical sequence as follows: 1-225: Missing.
Note: Due to intron retention. No experimental confirmation available.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 506

506

CDK5 regulatory subunit-associated protein 3

PRO_0000220516

Natural variations

Alternative sequence

1 – 225

225

Missing in isoform 3.

VSP_007568

Alternative sequence

1 – 87

87

Missing in isoform 2.

VSP_007566

Alternative sequence

88 – 111

24

RYSSQ…DNTYL → MCVHPGACLPHVGVSWAEFP GHFS in isoform 2.

VSP_007567

Natural variant

324

1

L → V: dbSNP rs35054799.

VAR_048688

Experimental info

Sequence conflict

113

1

E → V in BAD97107. Ref.6

Sequence conflict

406

1

E → K in AAK69655. Ref.3

Sequence conflict

443

1

Q → E in BAD97107. Ref.6

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified June 16, 2003. Version 2. Checksum: A21E59A79E1F87E0 Show »	FASTA	506	56,921
10 20 30 40 50 60 MEDHQHVPID IQTSKLLDWL VDRRHCSLKW QSLVLTIREK INAAIQDMPE SEEIAQLLSG 70 80 90 100 110 120 SYIHYFHCLR ILDLLKGTEA STKNIFGRYS SQRMKDWQEI IALYEKDNTY LVELSSLLVR 130 140 150 160 170 180 NVNYEIPSLK KQIAKCQQLQ QEYSRKEEEC QAGAAEMREQ FYHSCKQYGI TGENVRGELL 190 200 210 220 230 240 ALVKDLPSQL AEIGAAAQQS LGEAIDVYQA SVGFVCESPT EQVLPMLRFV QKRGNSTVYE 250 260 270 280 290 300 WRTGTEPSVV ERPHLEELPE QVAEDAIDWG DFGVEAVSEG TDSGISAEAA GIDWGIFPES 310 320 330 340 350 360 DSKDPGGDGI DWGDDAVALQ ITVLEAGTQA PEGVARGPDA LTLLEYTETR NQFLDELMEL 370 380 390 400 410 420 EIFLAQRAVE LSEEADVLSV SQFQLAPAIL QGQTKEKMVT MVSVLEDLIG KLTSLQLQHL 430 440 450 460 470 480 FMILASPRYV DRVTEFLQQK LKQSQLLALK KELMVQKQQE ALEEQAALEP KLDLLLEKTK 490 500 ELQKLIEADI SKRYSGRPVN LMGTSL « Hide
Isoform 2 (IC53). Checksum: DA15DED087A5A795 Show »	FASTA	419	46,351
10 20 30 40 50 60 MCVHPGACLP HVGVSWAEFP GHFSVELSSL LVRNVNYEIP SLKKQIAKCQ QLQQEYSRKE 70 80 90 100 110 120 EECQAGAAEM REQFYHSCKQ YGITGENVRG ELLALVKDLP SQLAEIGAAA QQSLGEAIDV 130 140 150 160 170 180 YQASVGFVCE SPTEQVLPML RFVQKRGNST VYEWRTGTEP SVVERPHLEE LPEQVAEDAI 190 200 210 220 230 240 DWGDFGVEAV SEGTDSGISA EAAGIDWGIF PESDSKDPGG DGIDWGDDAV ALQITVLEAG 250 260 270 280 290 300 TQAPEGVARG PDALTLLEYT ETRNQFLDEL MELEIFLAQR AVELSEEADV LSVSQFQLAP 310 320 330 340 350 360 AILQGQTKEK MVTMVSVLED LIGKLTSLQL QHLFMILASP RYVDRVTEFL QQKLKQSQLL 370 380 390 400 410 ALKKELMVQK QQEALEEQAA LEPKLDLLLE KTKELQKLIE ADISKRYSGR PVNLMGTSL « Hide
Isoform 3 (IC53-2). Checksum: BDC6C94A65DF078C Show »	FASTA	281	31,200
10 20 30 40 50 60 MLRFVQKRGN STVYEWRTGT EPSVVERPHL EELPEQVAED AIDWGDFGVE AVSEGTDSGI 70 80 90 100 110 120 SAEAAGIDWG IFPESDSKDP GGDGIDWGDD AVALQITVLE AGTQAPEGVA RGPDALTLLE 130 140 150 160 170 180 YTETRNQFLD ELMELEIFLA QRAVELSEEA DVLSVSQFQL APAILQGQTK EKMVTMVSVL 190 200 210 220 230 240 EDLIGKLTSL QLQHLFMILA SPRYVDRVTE FLQQKLKQSQ LLALKKELMV QKQQEALEEQ 250 260 270 280 AALEPKLDLL LEKTKELQKL IEADISKRYS GRPVNLMGTS L « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel gene IC53 stimulates ECV304 cell proliferation and is upregulated in failing heart." Chen J., Liu B., Liu Y.Q., Han Y., Yu H., Zhang Y., Lu L., Zhen Y., Hui R.T. Biochem. Biophys. Res. Commun. 294:161-166(2002) [PubMed: 12054757] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Aorta.
[2]	"Cloning and characterization of human IC53-2, a novel CDK5 activator binding protein." Xie Y.H., He X.H., Tang Y.T., Li J.J., Pan Z.M., Qin W.X., Wan da F., Gu J.R. Cell Res. 13:83-91(2003) [PubMed: 12737517] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, TISSUE SPECIFICITY. Tissue: Placenta.
[3]	"Protein interacting with the receptor binding domain of enteric adenovirus serotype 41 fiber protein." Favier A.-L., Harsi C., Chrobozcek J. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Kidney.
[4]	"Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients." Shichijo S., Itoh K. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon adenocarcinoma.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[6]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Synovial cell.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[8]	"Cloning of three novel neuronal Cdk5 activator binding proteins." Ching Y.-P., Qi Z., Wang J.H. Gene 242:285-294(2000) [PubMed: 10721722] [Abstract] Cited for: TISSUE SPECIFICITY.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AF110322 mRNA. Translation: AAG39277.1. AF217982 mRNA. Translation: AAG17225.1. AF343090 mRNA. Translation: AAK69655.1. Frameshift. AB062433 mRNA. Translation: BAB93496.1. AK023722 mRNA. Translation: BAB14657.1. AK223387 mRNA. Translation: BAD97107.1. BC009957 mRNA. Translation: AAH09957.1. BC072435 mRNA. Translation: AAH72435.1.
IPI	IPI00018780. IPI00187145. IPI00414442.
PIR	JC7863.
RefSeq	NP_788276.1.
UniGene	Hs.20157
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96JB5. 26 interactions.
PTM databases
PhosphoSite	Q96JB5.
Proteomic databases
PRIDE	Q96JB5.
Genome annotation databases
Ensembl	ENSG00000108465. Homo sapiens. [Contig view]
GeneID	80279.
KEGG	hsa:80279.
UCSC	uc002imq.1. human. uc002imr.1. human.
Organism-specific databases
GeneCards	GC17P043403.
H-InvDB	HIX0013932.
HGNC	HGNC:18673. CDK5RAP3.
MIM	608202. gene.
PharmGKB	PA38633.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q96JB5.
HOVERGEN	Q96JB5.
OMA	Q96JB5. SCKQYGI.
Gene expression databases
ArrayExpress	Q96JB5.
Bgee	Q96JB5.
CleanEx	HS_CDK5RAP3.
GermOnline	ENSG00000108465. Homo sapiens.
Family and domain databases
InterPro	IPR008491. DUF773. [Graphical view]
PANTHER	PTHR14894. DUF773. 1 hit.
Pfam	PF05600. DUF773. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	70750.
SOURCE	Search...

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Entry information

Entry name

CK5P3_HUMAN

Accession

Primary (citable) accession number: Q96JB5
Secondary accession number(s): Q53FA2

Q9HBR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 16, 2003
Last modified:	July 7, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Human chromosome 17: entries, gene names and cross-references to MIM

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List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents