Reviewed, UniProtKB/Swiss-Prot Q96JB3 (HIC2_HUMAN)

Names and origin

Protein names

Recommended name:
    Hypermethylated in cancer 2 protein
      Short name=Hic-2
Alternative name(s):
    Hic-3
    HIC1-related gene on chromosome 22
    Zinc finger and BTB domain-containing protein 30

Gene names

Name:	HIC2
Synonyms:	HRG22, KIAA1020, ZBTB30

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	615 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcriptional repressor.
Subunit structure	Interacts with CtBP.
Subcellular location	Nucleus.
Tissue specificity	Highest levels in cerebellum.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily. Contains 1 BTB (POZ) domain. Contains 5 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
Gene Ontology (GO)
Biological process	negative regulation of transcription, DNA-dependent Ref.1 Non-traceable author statement. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	DNA binding Ref.1 Non-traceable author statement. Source: UniProtKB protein C-terminus binding Inferred from physical interaction. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96JB3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96JB3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 615	615	Hypermethylated in cancer 2 protein		PRO_0000046945
Regions
	Domain	46 – 109	64	BTB
	Zinc finger	442 – 469	28	C2H2-type 1
	Zinc finger	505 – 532	28	C2H2-type 2
	Zinc finger	533 – 560	28	C2H2-type 3
	Zinc finger	561 – 588	28	C2H2-type 4
	Zinc finger	589 – 615	27	C2H2-type 5
	Region	246 – 250	5	Binding to CtBP
Natural variations
	Alternative sequence	1 – 18	18	Missing in isoform 2.		VSP_006829
Experimental info
	Sequence conflict	49 – 51	3	IIM → TIR in CAC70715. Ref.1
	Sequence conflict	176	1	Q → R in BAA82972. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 2, 2002. Version 2. Checksum: B0368C631B198C95	FASTA	615	66,156
	10 20 30 40 50 60 MVSGPLALRW CAWAGRGDMG PDMELPSHSK QLLLQLNQQR TKGFLCDVII MVENSIFRAH 70 80 90 100 110 120 KNVLAASSIY FKSLVLHDNL INLDTDMVSS TVFQQILDFI YTGKLLPSDQ PAEPNFSTLL 130 140 150 160 170 180 TAASYLQLPE LAALCRRKLK RAGKPFGSGR AGSTGMGRPP RSQRLSTASV IQARYQGLVD 190 200 210 220 230 240 GRKGAHAPQE LPQAKGSDDE LFLGGSNQDS VQGLGRAVCP AGGEAGLGGC SSSTNGSSGG 250 260 270 280 290 300 CEQELGLDLS KKSPPLPPAT PGPHLTPDDA AQLSDSQHGS PPAASAPPVA NSASYSELGG 310 320 330 340 350 360 TPDEPMDLEG AEDNHLSLLE APGGQPRKSL RHSTRKKEWG KKEPVAGSPF ERREAGPKGP 370 380 390 400 410 420 CPGEEGEGVG DRVPNGILAS GAGPSGPYGE PPYPCKEEEE NGKDASEDSA QSGSEGGSGH 430 440 450 460 470 480 ASAHYMYRQE GYETVSYGDN LYVCIPCAKG FPSSEQLNAH VETHTEEELF IKEEGAYETG 490 500 510 520 530 540 SGGAEEEAED LSAPSAAYTA EPRPFKCSVC EKTYKDPATL RQHEKTHWLT RPFPCNICGK 550 560 570 580 590 600 MFTQRGTMTR HMRSHLGLKP FACDECGMRF TRQYRLTEHM RVHSGEKPYE CQLCGGKFTQ 610 QRNLISHLRM HTSPS
	Isoform 2. Checksum: C536567FC3A1AAC8	FASTA	597	64,228
	10 20 30 40 50 60 MGPDMELPSH SKQLLLQLNQ QRTKGFLCDV IIMVENSIFR AHKNVLAASS IYFKSLVLHD 70 80 90 100 110 120 NLINLDTDMV SSTVFQQILD FIYTGKLLPS DQPAEPNFST LLTAASYLQL PELAALCRRK 130 140 150 160 170 180 LKRAGKPFGS GRAGSTGMGR PPRSQRLSTA SVIQARYQGL VDGRKGAHAP QELPQAKGSD 190 200 210 220 230 240 DELFLGGSNQ DSVQGLGRAV CPAGGEAGLG GCSSSTNGSS GGCEQELGLD LSKKSPPLPP 250 260 270 280 290 300 ATPGPHLTPD DAAQLSDSQH GSPPAASAPP VANSASYSEL GGTPDEPMDL EGAEDNHLSL 310 320 330 340 350 360 LEAPGGQPRK SLRHSTRKKE WGKKEPVAGS PFERREAGPK GPCPGEEGEG VGDRVPNGIL 370 380 390 400 410 420 ASGAGPSGPY GEPPYPCKEE EENGKDASED SAQSGSEGGS GHASAHYMYR QEGYETVSYG 430 440 450 460 470 480 DNLYVCIPCA KGFPSSEQLN AHVETHTEEE LFIKEEGAYE TGSGGAEEEA EDLSAPSAAY 490 500 510 520 530 540 TAEPRPFKCS VCEKTYKDPA TLRQHEKTHW LTRPFPCNIC GKMFTQRGTM TRHMRSHLGL 550 560 570 580 590 KPFACDECGM RFTRQYRLTE HMRVHSGEKP YECQLCGGKF TQQRNLISHL RMHTSPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of HRG22, a human homologue of the putative tumor suppressor gene HIC1." Deltour S., Pinte S., Guerardel C., Leprince D. Biochem. Biophys. Res. Commun. 287:427-434(2001) [PubMed: 11554746] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-85 (ISOFORM 1), ALTERNATIVE SPLICING.
[2]	"Complete deduced structure of HIC-3, a novel human btb/poz and ZF factor of the HIC family." Alliel P.M., Goudou D., Bitoun M., Seddiqi N., Rieger F., Perin J.-P. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:197-205(1999) [PubMed: 10470851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract] Cited for: SEQUENCE REVISION.
[5]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004) [PubMed: 15461802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed: 10591208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[8]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 301-615. Tissue: Testis.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ313204 mRNA. Translation: CAC70715.1. AF349035 mRNA. Translation: AAK72951.1. AB028943 mRNA. Translation: BAA82972.2. Different initiation. CR456377 mRNA. Translation: CAG30263.1. AP000557 Genomic DNA. No translation available. BC094787 mRNA. Translation: AAH94787.1. AL162003 mRNA. Translation: CAB82344.1.
IPI	IPI00219839. IPI00376570.
PIR	T47181.
RefSeq	NP_055909.2.
UniGene	Hs.632767
3D structure databases
HSSP	HSSP built from PDB template 1A1H based on UniProtKB P08046.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96JB3. 2 interactions.
PTM databases
PhosphoSite	Q96JB3.
Proteomic databases
PRIDE	Q96JB3.
Genome annotation databases
Ensembl	ENSG00000169635. Homo sapiens. [Contig view]
GeneID	23119.
KEGG	hsa:23119.
Organism-specific databases
GeneCards	GC22P020096.
H-InvDB	HIX0016273.
HGNC	HGNC:18595. HIC2.
MIM	607712. gene.
PharmGKB	PA26466.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q96JB3.
HOVERGEN	Q96JB3.
OMA	Q96JB3. GDRVPNG.
Gene expression databases
ArrayExpress	Q96JB3.
Bgee	Q96JB3.
CleanEx	HS_HIC2.
GermOnline	ENSG00000169635. Homo sapiens.
Family and domain databases
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR013069. BTB_POZ. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit. G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 2 hits.
Pfam	PF00651. BTB. 1 hit. PF00096. zf-C2H2. 4 hits. [Graphical view]
ProDom	PD000003. Znf_C2H2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00225. BTB. 1 hit. SM00355. ZnF_C2H2. 5 hits. [Graphical view]
PROSITE	PS50097. BTB. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 5 hits. PS50157. ZINC_FINGER_C2H2_2. 5 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	44339.
SOURCE	Search...

Entry information

Entry name

HIC2_HUMAN

Accession

Primary (citable) accession number: Q96JB3
Secondary accession number(s): Q504T6 , Q96KR3, Q9NSM9, Q9UPX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	May 2, 2002
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)