Reviewed, UniProtKB/Swiss-Prot Q96JB2 (COG3_HUMAN)

Names and origin

Protein names

Recommended name:
    Conserved oligomeric Golgi complex subunit 3
      Short name=COG complex subunit 3
Alternative name(s):
    Component of oligomeric Golgi complex 3
    Vesicle-docking protein SEC34 homolog
    p94

Gene names

Name:	COG3
Synonyms:	SEC34

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	828 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in ER-Golgi transport. Ref.2
Subunit structure	Component of the conserved oligomeric Golgi complex which is composed of eight different subunits and is required for normal Golgi morphology and localization By similarity.
Subcellular location	Golgi apparatus › Golgi stack membrane; Peripheral membrane protein. Note: Associated with the peripheral membrane of cis/medial cisternae. Ref.1
Tissue specificity	Widely expressed with highest levels in pancreas and testis and lowest levels in lung. Ref.1
Sequence similarities	Belongs to the COG3 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Ref.2 Inferred from direct assay. Source: UniProtKB intra-Golgi vesicle-mediated transport Ref.2 Inferred from direct assay. Source: UniProtKB intracellular protein transport Inferred from electronic annotation. Source: InterPro protein amino acid glycosylation Inferred from mutant phenotype. Source: UniProtKB protein localization in organelle Inferred from mutant phenotype. Source: UniProtKB protein stabilization Inferred from mutant phenotype. Source: UniProtKB retrograde vesicle-mediated transport, Golgi to ER Inferred from mutant phenotype. Source: UniProtKB
Cellular component	Golgi transport complex Ref.1 Inferred from direct assay. Source: UniProtKB cis-Golgi network Inferred from electronic annotation. Source: InterPro extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB protein transporter activity Ref.2 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96JB2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96JB2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     443-444: AE → GK
     445-828: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 828	827	Conserved oligomeric Golgi complex subunit 3		PRO_0000213500
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.6
	Modified residue	533	1	Phosphoserine Ref.7
	Modified residue	663	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	443 – 444	2	AE → GK in isoform 2.		VSP_013652
	Alternative sequence	445 – 828	384	Missing in isoform 2.		VSP_013653
	Natural variant	620	1	R → C in a breast cancer sample; somatic mutation. Ref.11		VAR_036454
	Natural variant	747	1	N → S: dbSNP rs2274285. Ref.2		VAR_055663
Experimental info
	Sequence conflict	106 – 107	2	QQ → HE in AAK66974. Ref.1
	Sequence conflict	825	1	L → S in AAK66974. Ref.1
	Sequence conflict	825	1	L → S in AAK06848. Ref.2
	Sequence conflict	825	1	L → S in BAG37012. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: B3DCF03FD4DC6A60	FASTA	828	94,096
	10 20 30 40 50 60 MAEAALLLLP EAAAERDARE KLALWDRRPD TTAPLTDRQT DSVLELKAAA ENLPVPAELP 70 80 90 100 110 120 IEDLCSLTSQ SLPIELTSVV PESTEDILLK GFTSLGMEEE RIETAQQFFS WFAKLQTQMD 130 140 150 160 170 180 QDEGTKYRQM RDYLSGFQEQ CDAILNDVNS ALQHLESLQK QYLFVSNKTG TLHEACEQLL 190 200 210 220 230 240 KEQSELVDLA ENIQQKLSYF NELETINTKL NSPTLSVNSD GFIPMLAKLD DCITYISSHP 250 260 270 280 290 300 NFKDYPIYLL KFKQCLSKAL HLMKTYTVNT LQTLTSQLLK RDPSSVPNAD NAFTLFYVKF 310 320 330 340 350 360 RAAAPKVRTL IEQIELRSEK IPEYQQLLND IHQCYLDQRE LLLGPSIACT VAELTSQNNR 370 380 390 400 410 420 DHCALVRSGC AFMVHVCQDE HQLYNEFFTK PTSKLDELLE KLCVSLYDVF RPLIIHVIHL 430 440 450 460 470 480 ETLSELCGIL KNEVLEDHVQ NNAEQLGAFA AGVKQMLEDV QERLVYRTHI YIQTDITGYK 490 500 510 520 530 540 PAPGDLAYPD KLVMMEQIAQ SLKDEQKKVP SEASFSDVHL EEGESNSLTK SGSTESLNPR 550 560 570 580 590 600 PQTTISPADL HGMWYPTVRR TLVCLSKLYR CIDRAVFQGL SQEALSACIQ SLLGASESIS 610 620 630 640 650 660 KNKTQIDGQL FLIKHLLILR EQIAPFHTEF TIKEISLDLK KTRDAAFKIL NPMTVPRFFR 670 680 690 700 710 720 LNSNNALIEF LLEGTPEIRE HYLDSKKDVD RHLKSACEQF IQQQTKLFVE QLEEFMTKVS 730 740 750 760 770 780 ALKTMASQGG PKYTLSQQPW AQPAKVNDLA ATAYKTIKTK LPVTLRSMSL YLSNKDTEFI 790 800 810 820 LFKPVRNNIQ QVFQKFHALL KEEFSPEDIQ IIACPSMEQL SLLLLVSK
	Isoform 2. Checksum: 55874C370679512F	FASTA	444	50,540
	10 20 30 40 50 60 MAEAALLLLP EAAAERDARE KLALWDRRPD TTAPLTDRQT DSVLELKAAA ENLPVPAELP 70 80 90 100 110 120 IEDLCSLTSQ SLPIELTSVV PESTEDILLK GFTSLGMEEE RIETAQQFFS WFAKLQTQMD 130 140 150 160 170 180 QDEGTKYRQM RDYLSGFQEQ CDAILNDVNS ALQHLESLQK QYLFVSNKTG TLHEACEQLL 190 200 210 220 230 240 KEQSELVDLA ENIQQKLSYF NELETINTKL NSPTLSVNSD GFIPMLAKLD DCITYISSHP 250 260 270 280 290 300 NFKDYPIYLL KFKQCLSKAL HLMKTYTVNT LQTLTSQLLK RDPSSVPNAD NAFTLFYVKF 310 320 330 340 350 360 RAAAPKVRTL IEQIELRSEK IPEYQQLLND IHQCYLDQRE LLLGPSIACT VAELTSQNNR 370 380 390 400 410 420 DHCALVRSGC AFMVHVCQDE HQLYNEFFTK PTSKLDELLE KLCVSLYDVF RPLIIHVIHL 430 440 ETLSELCGIL KNEVLEDHVQ NNGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a human ortholog of Sec34p as a component of the cis-Golgi vesicle tethering machinery." Suvorova E.S., Kurten R.C., Lupashin V.V. J. Biol. Chem. 276:22810-22818(2001) [PubMed: 11292827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Fetal brain.
[2]	"Sec34 is implicated in traffic from the endoplasmic reticulum to the Golgi and exists in a complex with GTC-90 and ldlBp." Loh E., Hong W. J. Biol. Chem. 277:21955-21961(2002) [PubMed: 11929878] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, VARIANT SER-747.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[4]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Leukocyte.
[6]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-16, ACETYLATION AT ALA-2. Tissue: Platelet.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-533 AND SER-663, MASS SPECTROMETRY.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[9]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-663, MASS SPECTROMETRY. Tissue: T-cell.
[11]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-620.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF349676 mRNA. Translation: AAK66974.1. AF332595 mRNA. Translation: AAK06848.1. AK314387 mRNA. Translation: BAG37012.1. AL139326, AL606514 Genomic DNA. Translation: CAH72888.1. BC038953 mRNA. Translation: AAH38953.1.
IPI	IPI00414858. IPI00555771.
RefSeq	NP_113619.1.
UniGene	Hs.507948
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q96JB2.
PTM databases
PhosphoSite	Q96JB2.
Proteomic databases
PRIDE	Q96JB2.
Genome annotation databases
Ensembl	ENST00000349995; ENSP00000258654; ENSG00000136152; Homo sapiens. [Genome view]
GeneID	83548.
KEGG	hsa:83548.
NMPDR	fig|9606.3.peg.8816.
UCSC	uc001vai.2. human. uc001vak.1. human.
Organism-specific databases
CTD	83548.
GeneCards	GC13P044937.
H-InvDB	HIX0011290.
HGNC	HGNC:18619. COG3.
MIM	606975. gene.
PharmGKB	PA38602.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q96JB2.
HOVERGEN	Q96JB2.
OMA	YISSHPN
OrthoDB	EOG9SBHJD
Gene expression databases
ArrayExpress	Q96JB2.
Bgee	Q96JB2.
CleanEx	HS_COG3.
Genevestigator	Q96JB2.
GermOnline	ENSG00000136152. Homo sapiens.
Family and domain databases
InterPro	IPR007265. Sec34. [Graphical view]
PANTHER	PTHR13302. Sec34. 1 hit.
Pfam	PF04136. Sec34. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	72485.
SOURCE	Search...

Entry information

Entry name

COG3_HUMAN

Accession

Primary (citable) accession number: Q96JB2
Secondary accession number(s): B2RAW5 , Q5VT70, Q8IXX4, Q9BZ92

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.