Q96J92 (WNK4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase WNK4
EC=2.7.11.1

Alternative name(s):
Protein kinase lysine-deficient 4
Protein kinase with no lysine 4

Gene names

Name:	WNK4
Synonyms:	PRKWNK4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1243 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K+ channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K+ secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Ref.5
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity. UniProtKB Q9H4A3
Enzyme regulation	Activation requires autophosphorylation of Ser-335. Phosphorylation of Ser-331 also promotes increased activity By similarity. UniProtKB Q9JIH7
Subunit structure	Interacts with the C-terminal region of KCNJ1 By similarity. Interacts with WNK1 and WNK3 By similarity. UniProtKB Q80UE6
Subcellular location	Cell junction › tight junction By similarity. Note: Present exclusively in intercellular junctions in the distal convoluted tubule and in both the cytoplasm and intercellular junctions in the cortical collecting duct. WNK4 is part of the tight junction complex By similarity.
Tissue specificity	Expressed in kidney, colon and skin. Ref.4
Post-translational modification	Phosphorylated by WNK1 and WNK3 By similarity.
Involvement in disease	Pseudohypoaldosteronism 2B (PHA2B) [MIM:614491]: An autosomal dominant disorder characterized by hypertension, hyperkalemia, hyperchloremia, mild hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. Contains 1 protein kinase domain.
Caution	Cys-203 is present instead of the conserved Lys which is expected to be an active site residue. Lys-186 appears to fulfill the required catalytic function. UniProtKB Q9JIH7
Sequence caution	The sequence BAC04669.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAC48387.1 differs from that shown. Reason: Frameshift at position 4.

Ontologies

Keywords
Cellular component	Cell junction Tight junction
Coding sequence diversity	Alternative splicing Polymorphism
Disease	Disease mutation
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chloride transport Inferred from electronic annotation. Source: Compara intracellular protein kinase cascade Inferred from sequence or structural similarity. Source: UniProtKB ion transport Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of pancreatic juice secretion Inferred from electronic annotation. Source: Compara protein localization Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara tight junction Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from sequence or structural similarity. Source: UniProtKB protein serine/threonine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: Q96J92-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96J92-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-228: Missing.
     229-264: QHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKT → MRRRQQGAAGGNFPVGGSFPEDVSPHQDSGYAPSPR
     828-1210: Missing.
     1233-1243: GVTFAGDVGRM → EAGQRPGKLWLRATVQLRVWGLELRRKEMMGRNPKLGAAPNP

Note: No experimental confirmation available.

Isoform 3 (identifier: Q96J92-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1145-1165: HLSEVETLQTLQKKEIEDLYS → PFHALRASSGTCQRWKHYRHY
     1166-1243: Missing.

Note: No experimental confirmation available. Incomplete sequence.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1243	1243	Serine/threonine-protein kinase WNK4		PRO_0000086824
Regions
	Domain	174 – 432	259	Protein kinase
	Nucleotide binding	180 – 188	9	ATP By similarity UniProtKB Q8TDX7
Sites
	Active site	302	1	Proton acceptor By similarity UniProtKB Q8TDX7
	Binding site	186	1	ATP By similarity UniProtKB Q9JIH7
Amino acid modifications
	Modified residue	331	1	Phosphoserine; by autocatalysis By similarity UniProtKB Q9JIH7
	Modified residue	335	1	Phosphoserine; by autocatalysis By similarity UniProtKB Q9JIH7
Natural variations
	Alternative sequence	1 – 228	228	Missing in isoform 2.		VSP_050648
	Alternative sequence	229 – 264	36	QHPNI…GTLKT → MRRRQQGAAGGNFPVGGSFP EDVSPHQDSGYAPSPR in isoform 2.		VSP_050649
	Alternative sequence	828 – 1210	383	Missing in isoform 2.		VSP_050650
	Alternative sequence	1145 – 1165	21	HLSEV…EDLYS → PFHALRASSGTCQRWKHYRH Y in isoform 3.		VSP_050652
	Alternative sequence	1166 – 1243	78	Missing in isoform 3.		VSP_050653
	Alternative sequence	1233 – 1243	11	GVTFAGDVGRM → EAGQRPGKLWLRATVQLRVW GLELRRKEMMGRNPKLGAAP NP in isoform 2.		VSP_050651
	Natural variant	434	1	E → D in an ovarian mucinous carcinoma sample; somatic mutation. Ref.6		VAR_041330
	Natural variant	562	1	E → K in PHA2B. Ref.1		VAR_017588
	Natural variant	564	1	D → A in PHA2B. Ref.1		VAR_017589
	Natural variant	565	1	Q → E in PHA2B. Ref.1		VAR_017590
	Natural variant	601	1	A → S. Corresponds to variant rs55781437 [ dbSNP | Ensembl ].		VAR_061748
	Natural variant	677	1	R → W. Corresponds to variant rs9896991 [ dbSNP | Ensembl ].		VAR_051685
	Natural variant	813	1	P → L. Ref.6		VAR_041331
	Natural variant	961	1	P → S. Corresponds to variant rs2290041 [ dbSNP | Ensembl ].		VAR_051686
	Natural variant	992	1	P → S in a metastatic melanoma sample; somatic mutation. Ref.6		VAR_041332
	Natural variant	1013	1	L → P. Ref.6		VAR_041333
	Natural variant	1185	1	R → C in PHA2B. Ref.1		VAR_017591
Experimental info
	Sequence conflict	525	1	R → C in BAC04688. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: BAC35F0098BA3391	FASTA	1,243	134,739
	10 20 30 40 50 60 MLASPATETT VLMSQTEADL ALRPPPPLGT AGQPRLGPPP RRARRFSGKA EPRPRSSRLS 70 80 90 100 110 120 RRSSVDLGLL SSWSLPASPA PDPPDPPDSA GPGPARSPPP SSKEPPEGTW TEGAPVKAAE 130 140 150 160 170 180 DSARPELPDS AVGPGSREPL RVPEAVALER RREQEEKEDM ETQAVATSPD GRYLKFDIEI 190 200 210 220 230 240 GRGSFKTVYR GLDTDTTVEV AWCELQTRKL SRAERQRFSE EVEMLKGLQH PNIVRFYDSW 250 260 270 280 290 300 KSVLRGQVCI VLVTELMTSG TLKTYLRRFR EMKPRVLQRW SRQILRGLHF LHSRVPPILH 310 320 330 340 350 360 RDLKCDNVFI TGPTGSVKIG DLGLATLKRA SFAKSVIGTP EFMAPEMYEE KYDEAVDVYA 370 380 390 400 410 420 FGMCMLEMAT SEYPYSECQN AAQIYRKVTS GRKPNSFHKV KIPEVKEIIE GCIRTDKNER 430 440 450 460 470 480 FTIQDLLAHA FFREERGVHV ELAEEDDGEK PGLKLWLRME DARRGGRPRD NQAIEFLFQL 490 500 510 520 530 540 GRDAAEEVAQ EMVALGLVCE ADYQPVARAV RERVAAIQRK REKLRKAREL EALPPEPGPP 550 560 570 580 590 600 PATVPMAPGP PSVFPPEPEE PEADQHQPFL FRHASYSSTT SDCETDGYLS SSGFLDASDP 610 620 630 640 650 660 ALQPPGGVPS SLAESHLCLP SAFALSIPRS GPGSDFSPGD SYASDAASGL SDVGEGMGQM 670 680 690 700 710 720 RRPPGRNLRR RPRSRLRVTS VSDQNDRVVE CQLQTHNSKM VTFRFDLDGD SPEEIAAAMV 730 740 750 760 770 780 YNEFILPSER DGFLRRIREI IQRVETLLKR DTGPMEAAED TLSPQEEPAP LPALPVPLPD 790 800 810 820 830 840 PSNEELQSST SLEHRSWTAF STSSSSPGTP LSPGNPFSPG TPISPGPIFP ITSPPCHPSP 850 860 870 880 890 900 SPFSPISSQV SSNPSPHPTS SPLPFSSSTP EFPVPLSQCP WSSLPTTSPP TFSPTCSQVT 910 920 930 940 950 960 LSSPFFPPCP STSSFPSTTA APLLSLASAF SLAVMTVAQS LLSPSPGLLS QSPPAPPSPL 970 980 990 1000 1010 1020 PSLPLPPPVA PGGQESPSPH TAEVESEASP PPARPLPGEA RLAPISEEGK PQLVGRFQVT 1030 1040 1050 1060 1070 1080 SSKEPAEPLP LQPTSPTLSG SPKPSTPQLT SESSDTEDSA GGGPETREAL AESDRAAEGL 1090 1100 1110 1120 1130 1140 GAGVEEEGDD GKEPQVGGSP QPLSHPSPVW MNYSYSSLCL SSEESESSGE DEEFWAELQS 1150 1160 1170 1180 1190 1200 LRQKHLSEVE TLQTLQKKEI EDLYSRLGKQ PPPGIVAPAA MLSSRQRRLS KGSFPTSRRN 1210 1220 1230 1240 SLQRSEPPGP GIMRRNSLSG SSTGSQEQRA SKGVTFAGDV GRM
	Isoform 2 [UniParc]. Checksum: 6711A9D6CEFD8146	FASTA	663	73,370
	10 20 30 40 50 60 MRRRQQGAAG GNFPVGGSFP EDVSPHQDSG YAPSPRYLRR FREMKPRVLQ RWSRQILRGL 70 80 90 100 110 120 HFLHSRVPPI LHRDLKCDNV FITGPTGSVK IGDLGLATLK RASFAKSVIG TPEFMAPEMY 130 140 150 160 170 180 EEKYDEAVDV YAFGMCMLEM ATSEYPYSEC QNAAQIYRKV TSGRKPNSFH KVKIPEVKEI 190 200 210 220 230 240 IEGCIRTDKN ERFTIQDLLA HAFFREERGV HVELAEEDDG EKPGLKLWLR MEDARRGGRP 250 260 270 280 290 300 RDNQAIEFLF QLGRDAAEEV AQEMVALGLV CEADYQPVAR AVRERVAAIQ RKREKLRKAR 310 320 330 340 350 360 ELEALPPEPG PPPATVPMAP GPPSVFPPEP EEPEADQHQP FLFRHASYSS TTSDCETDGY 370 380 390 400 410 420 LSSSGFLDAS DPALQPPGGV PSSLAESHLC LPSAFALSIP RSGPGSDFSP GDSYASDAAS 430 440 450 460 470 480 GLSDVGEGMG QMRRPPGRNL RRRPRSRLRV TSVSDQNDRV VECQLQTHNS KMVTFRFDLD 490 500 510 520 530 540 GDSPEEIAAA MVYNEFILPS ERDGFLRRIR EIIQRVETLL KRDTGPMEAA EDTLSPQEEP 550 560 570 580 590 600 APLPALPVPL PDPSNEELQS STSLEHRSWT AFSTSSSSPG TPLSPGNPFS PGTPISPGPG 610 620 630 640 650 660 IMRRNSLSGS STGSQEQRAS KEAGQRPGKL WLRATVQLRV WGLELRRKEM MGRNPKLGAA PNP
	Isoform 3 [UniParc]. Checksum: 87D926F2D3141A1B	FASTA	1,165	126,577
	10 20 30 40 50 60 MLASPATETT VLMSQTEADL ALRPPPPLGT AGQPRLGPPP RRARRFSGKA EPRPRSSRLS 70 80 90 100 110 120 RRSSVDLGLL SSWSLPASPA PDPPDPPDSA GPGPARSPPP SSKEPPEGTW TEGAPVKAAE 130 140 150 160 170 180 DSARPELPDS AVGPGSREPL RVPEAVALER RREQEEKEDM ETQAVATSPD GRYLKFDIEI 190 200 210 220 230 240 GRGSFKTVYR GLDTDTTVEV AWCELQTRKL SRAERQRFSE EVEMLKGLQH PNIVRFYDSW 250 260 270 280 290 300 KSVLRGQVCI VLVTELMTSG TLKTYLRRFR EMKPRVLQRW SRQILRGLHF LHSRVPPILH 310 320 330 340 350 360 RDLKCDNVFI TGPTGSVKIG DLGLATLKRA SFAKSVIGTP EFMAPEMYEE KYDEAVDVYA 370 380 390 400 410 420 FGMCMLEMAT SEYPYSECQN AAQIYRKVTS GRKPNSFHKV KIPEVKEIIE GCIRTDKNER 430 440 450 460 470 480 FTIQDLLAHA FFREERGVHV ELAEEDDGEK PGLKLWLRME DARRGGRPRD NQAIEFLFQL 490 500 510 520 530 540 GRDAAEEVAQ EMVALGLVCE ADYQPVARAV RERVAAIQRK REKLRKAREL EALPPEPGPP 550 560 570 580 590 600 PATVPMAPGP PSVFPPEPEE PEADQHQPFL FRHASYSSTT SDCETDGYLS SSGFLDASDP 610 620 630 640 650 660 ALQPPGGVPS SLAESHLCLP SAFALSIPRS GPGSDFSPGD SYASDAASGL SDVGEGMGQM 670 680 690 700 710 720 RRPPGRNLRR RPRSRLRVTS VSDQNDRVVE CQLQTHNSKM VTFRFDLDGD SPEEIAAAMV 730 740 750 760 770 780 YNEFILPSER DGFLRRIREI IQRVETLLKR DTGPMEAAED TLSPQEEPAP LPALPVPLPD 790 800 810 820 830 840 PSNEELQSST SLEHRSWTAF STSSSSPGTP LSPGNPFSPG TPISPGPIFP ITSPPCHPSP 850 860 870 880 890 900 SPFSPISSQV SSNPSPHPTS SPLPFSSSTP EFPVPLSQCP WSSLPTTSPP TFSPTCSQVT 910 920 930 940 950 960 LSSPFFPPCP STSSFPSTTA APLLSLASAF SLAVMTVAQS LLSPSPGLLS QSPPAPPSPL 970 980 990 1000 1010 1020 PSLPLPPPVA PGGQESPSPH TAEVESEASP PPARPLPGEA RLAPISEEGK PQLVGRFQVT 1030 1040 1050 1060 1070 1080 SSKEPAEPLP LQPTSPTLSG SPKPSTPQLT SESSDTEDSA GGGPETREAL AESDRAAEGL 1090 1100 1110 1120 1130 1140 GAGVEEEGDD GKEPQVGGSP QPLSHPSPVW MNYSYSSLCL SSEESESSGE DEEFWAELQS 1150 1160 LRQKPFHALR ASSGTCQRWK HYRHY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human hypertension caused by mutations in WNK kinases." Wilson F.H., Disse-Nicodeme S., Choate K.A., Ishikawa K., Nelson-Williams C., Desitter I., Gunel M., Milford D.V., Lipkin G.W., Achard J.-M., Feely M.P., Dussol B., Berland Y., Unwin R.J., Mayan H., Simon D.B., Farfel Z., Jeunemaitre X., Lifton R.P. Science 293:1107-1112(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DISEASE, VARIANTS PHA2B LYS-562; ALA-564; GLU-565 AND CYS-1185. Tissue: Kidney.
[2]	Chistiakov D.A. Thesis (2001), College de France / Paris, France Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Kidney.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 504-1165 (ISOFORM 3). Tissue: Kidney.
[4]	"WNK kinases, a novel protein kinase subfamily in multi-cellular organisms." Verissimo F., Jordan P. Oncogene 20:5562-5569(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 207-418 (ISOFORMS 1/3), TISSUE SPECIFICITY, CHROMOSOMAL LOCATION. Tissue: Colon.
[5]	"Serum and glucocorticoid-induced kinase (SGK) 1 and the epithelial sodium channel are regulated by multiple with no lysine (WNK) family members." Heise C.J., Xu B.E., Deaton S.L., Cha S.K., Cheng C.J., Earnest S., Sengupta S., Juang Y.C., Stippec S., Xu Y., Zhao Y., Huang C.L., Cobb M.H. J. Biol. Chem. 285:25161-25167(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] ASP-434; LEU-813; SER-992 AND PRO-1013.
+	Additional computationally mapped references.

Web resources

GeneReviews

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF390018 mRNA. Translation: AAK91995.1.
AJ316534 mRNA. Translation: CAC48387.1. Frameshift.
AK096003 mRNA. Translation: BAC04669.1. Different initiation.
AK096052 mRNA. Translation: BAC04688.1.
AJ309861 mRNA. Translation: CAC32991.1.

IPI

IPI00151141.
IPI00398442.
IPI00398443.

RefSeq

NP_115763.2. NM_032387.4.

UniGene

Hs.105448.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2V3S	X-ray	1.70	C/D	1015-1020	[»]

ProteinModelPortal

Q96J92.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96J92. 3 interactions.

MINT

MINT-5207492.

STRING

9606.ENSP00000246914.

PTM databases

PhosphoSite

Q96J92.

Polymorphism databases

DMDM

41688789.

Proteomic databases

PaxDb

Q96J92.

PRIDE

Q96J92.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000246914; ENSP00000246914; ENSG00000126562.

GeneID

65266.

KEGG

hsa:65266.

UCSC

uc002ibj.3. human.

Organism-specific databases

CTD

65266.

GeneCards

GC17P040932.

HGNC

HGNC:14544. WNK4.

HPA

HPA016500.

MIM

601844. gene.
614491. phenotype.

neXtProt

NX_Q96J92.

Orphanet

88939. Pseudohypoaldosteronism type 2B.

PharmGKB

PA134875400.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0515.

HOGENOM

HOG000230962.

HOVERGEN

HBG050347.

InParanoid

Q96J92.

KO

K08867.

OMA

WAELQNL.

OrthoDB

EOG4DFPN4.

PhylomeDB

Q96J92.

Enzyme and pathway databases

SignaLink

Q96J92.

Gene expression databases

ArrayExpress

Q96J92.

Bgee

Q96J92.

CleanEx

HS_WNK4.

Genevestigator

Q96J92.

GermOnline

ENSG00000126562. Homo sapiens.

Family and domain databases

InterPro

IPR011009. Kinase-like_dom.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]

Pfam

PF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]

SUPFAM

SSF56112. Kinase_like. 1 hit.

PROSITE

PS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChEMBL

CHEMBL1795196.

ChiTaRS

WNK4. human.

EvolutionaryTrace

Q96J92.

GenomeRNAi

65266.

NextBio

67398.

SOURCE

Search...

Entry information

Entry name

WNK4_HUMAN

Accession

Primary (citable) accession number: Q96J92
Secondary accession number(s): Q8N8X3 , Q8N8Z2, Q96DT8, Q9BYS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	December 1, 2001
Last modified:	May 29, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.