Reviewed, UniProtKB/Swiss-Prot Q96J87 (CELF6_HUMAN)

Names and origin

Protein names

Recommended name:
    CUG-BP- and ETR-3-like factor 6
      Short name=CELF-6
Alternative name(s):
    Bruno-like protein 6
    RNA-binding protein BRUNOL-6

Gene names

Name:	BRUNOL6
Synonyms:	CELF6

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in a muscle-specific splicing enhancer (MSE)-dependent manner. Promotes also exon exclusion of INSR pre-mRNA. Ref.5
Subcellular location	Nucleus By similarity. Cytoplasm By similarity.
Tissue specificity	Expressed mainly in kidney, brain and testis and present in other tissues albeit at lower levels. Also expressed in fetal kidney. Ref.5
Sequence similarities	Belongs to the CELF/BRUNOL family. Contains 3 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Ligand	RNA-binding
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW regulation of alternative nuclear mRNA splicing, via spliceosome Ref.5 Inferred from direct assay. Source: UniProtKB
Cellular component	cytoplasm Ref.5 Non-traceable author statement. Source: UniProtKB nucleus Ref.5 Non-traceable author statement. Source: UniProtKB
Molecular function	RNA binding Ref.5 Non-traceable author statement. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96J87-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96J87-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-113: Missing.
     114-131: PGMNRPIQVKPAASEGRG → MISDPPSNNSWNSVLYLL

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 481

481

CUG-BP- and ETR-3-like factor 6

PRO_0000295229

Regions

Domain

46 – 127

82

RRM 1

Domain

134 – 214

81

RRM 2

Domain

396 – 474

79

RRM 3

Compositional bias

242 – 295

54

Ala-rich

Natural variations

Alternative sequence

1 – 113

113

Missing in isoform 2.

VSP_026847

Alternative sequence

114 – 131

18

PGMNR…SEGRG → MISDPPSNNSWNSVLYLL in isoform 2.

VSP_026848

Natural variant

152

1

R → P: dbSNP rs34566074.

VAR_033265

Experimental info

Sequence conflict

2

1

A → T in AAH30835. Ref.4

Sequence conflict

469

1

V → A in AAH30835. Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

481

Helix Strand Turn

Details...

Beta strand

47 – 50

4

Helix

59 – 66

8

Beta strand

72 – 79

8

Beta strand

89 – 96

8

Helix

97 – 107

11

Turn

108 – 110

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: E11AF3BF1E7FC7A5	FASTA	481	50,477
	10 20 30 40 50 60 MAAAPGGSAQ PAGPGPRLGF STADSGVGMS GLNPGPAVPM KDHDAIKLFV GQIPRGLDEQ 70 80 90 100 110 120 DLKPLFEEFG RIYELTVLKD RLTGLHKGCA FLTYCARDSA LKAQSALHEQ KTLPGMNRPI 130 140 150 160 170 180 QVKPAASEGR GEDRKLFVGM LGKQQGEEDV RRLFQPFGHI EECTVLRSPD GTSKGCAFVK 190 200 210 220 230 240 FGSQGEAQAA IRGLHGSRTM AGASSSLVVK LADTDRERAL RRMQQMAGHL GAFHPAPLPL 250 260 270 280 290 300 GACGAYTTAI LQHQAALLAA AQGPGLGPVA AVAAQMQHVA AFSLVAAPLL PAAAANSPPG 310 320 330 340 350 360 SGPGTLPGLP APIGVNGFGP LTPQTNGQPG SDTLYNNGLS PYPAQSPGVA DPLQQAYAGM 370 380 390 400 410 420 HHYAAAYPSA YAPVSTAFPQ QPSALPQQQR EGPEGCNLFI YHLPQEFGDA ELIQTFLPFG 430 440 450 460 470 480 AVVSAKVFVD RATNQSKCFG FVSFDNPTSA QTAIQAMNGF QIGMKRLKVQ LKRPKDANRP Y
	Isoform 2. Checksum: D42E182AFE072BBC	FASTA	368	38,759
	10 20 30 40 50 60 MISDPPSNNS WNSVLYLLED RKLFVGMLGK QQGEEDVRRL FQPFGHIEEC TVLRSPDGTS 70 80 90 100 110 120 KGCAFVKFGS QGEAQAAIRG LHGSRTMAGA SSSLVVKLAD TDRERALRRM QQMAGHLGAF 130 140 150 160 170 180 HPAPLPLGAC GAYTTAILQH QAALLAAAQG PGLGPVAAVA AQMQHVAAFS LVAAPLLPAA 190 200 210 220 230 240 AANSPPGSGP GTLPGLPAPI GVNGFGPLTP QTNGQPGSDT LYNNGLSPYP AQSPGVADPL 250 260 270 280 290 300 QQAYAGMHHY AAAYPSAYAP VSTAFPQQPS ALPQQQREGP EGCNLFIYHL PQEFGDAELI 310 320 330 340 350 360 QTFLPFGAVV SAKVFVDRAT NQSKCFGFVS FDNPTSAQTA IQAMNGFQIG MKRLKVQLKR PKDANRPY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A family of human RNA-binding proteins related to the Drosophila Bruno translational regulator." Good P.J., Chen Q., Warner S.J., Herring D.C. J. Biol. Chem. 275:28583-28592(2000) [PubMed: 10893231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Frontal cortex.
[2]	"Characterization of the ELAV/BRUNO/TIA superfamily." Bozzali M., Quattrone S., Quattrone A. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Spleen.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain.
[5]	"CELF6, a member of the CELF family of RNA-binding proteins, regulates muscle-specific splicing enhancer-dependent alternative splicing." Ladd A.N., Nguyen N.H., Malhotra K., Cooper T.A. J. Biol. Chem. 279:17756-17764(2004) [PubMed: 14761971] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]	"Solution structure of the N-terminal RNA binding domain in Bruno-like 6 RNA-binding protein." RIKEN structural genomics initiative (RSGI) Submitted (SEP-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 31-132.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF401233 mRNA. Translation: AAK95615.1.
AF425606 mRNA. Translation: AAL34513.1.
AK131098 mRNA. Translation: BAC85148.1. Different initiation.
BC030835 mRNA. Translation: AAH30835.1.
BC033838 mRNA. Translation: AAH33838.1.

IPI

IPI00171103.
IPI00793692.

RefSeq

NP_443072.3.

UniGene

Hs.348342

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DGQ	NMR	-	A	38-132	[»]

ModBase

Search...

Proteomic databases

PRIDE

Q96J87.

Genome annotation databases

Ensembl

ENST00000287202; ENSP00000287202; ENSG00000140488; Homo sapiens. [Genome view]

GeneID

60677.

KEGG

hsa:60677.

UCSC

uc002auh.1. human.
uc002aui.1. human.

Organism-specific databases

CTD

60677.

GeneCards

GC15M070364.

HGNC

HGNC:14059. BRUNOL6.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

HBG619732.

HOVERGEN

Q96J87.

InParanoid

Q96J87.

OMA

GHIEECT.

OrthoDB

EOG9V19KN.

Gene expression databases

ArrayExpress

Q96J87.

Bgee

Q96J87.

CleanEx

HS_BRUNOL6.

Genevestigator

Q96J87.

Family and domain databases

InterPro

IPR012677. a_b_plait_nuc_bd.
IPR015903. RNP_BRUNO-like.
IPR000504. RRM_RNP1.
[Graphical view]

Gene3D

G3DSA:3.30.70.330. a_b_plait_nuc_bd. 3 hits.

PANTHER

PTHR10432:SF35. RNP_BRUNO-like. 1 hit.

Pfam

PF00076. RRM_1. 3 hits.
[Graphical view]

SMART

SM00360. RRM. 3 hits.
[Graphical view]

PROSITE

PS50102. RRM. 3 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

65492.

Entry information

Entry name

CELF6_HUMAN

Accession

Primary (citable) accession number: Q96J87
Secondary accession number(s): Q6PII4, Q6ZNJ7, Q8N607

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	December 1, 2001
Last modified:	December 15, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.