Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q96J65 (MRP9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multidrug resistance-associated protein 9
Alternative name(s):
ATP-binding cassette sub-family C member 12
Gene names
Name:ABCC12
Synonyms:MRP9
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1359 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable transporter By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Expressed in testis (at protein level). Widely expressed at low level. Isoform 5 is specifically expressed in brain, testis and breast cancer cells. Ref.1 Ref.2 Ref.3

Developmental stage

Expressed in fetal tissues. Ref.1

Sequence similarities

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96J65-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96J65-2)

Also known as: A; C; D;

The sequence of this isoform differs from the canonical sequence as follows:
     142-143: VI → TV
     734-736: Missing.
     1013-1359: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 3 (identifier: Q96J65-3)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     142-143: VI → TV
     734-736: Missing.
     892-938: ILKSPMSFFD...NFLQQFFMVV → HFPQRSPGAQ...WQEGELHHLV
     939-1359: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 4 (identifier: Q96J65-4)

The sequence of this isoform differs from the canonical sequence as follows:
     142-143: VI → TV
     572-633: YQHTVRVCGL...YLLDDPLSAV → LGSGASTSLG...RRSGERQSSW
     634-1359: Missing.
Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 5 (identifier: Q96J65-5)

The sequence of this isoform differs from the canonical sequence as follows:
     142-143: VI → TV
     220-277: Missing.
     737-760: Missing.
     1013-1359: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13591359Multidrug resistance-associated protein 9
PRO_0000253578

Regions

Transmembrane125 – 14521Helical; Potential
Transmembrane159 – 17921Helical; Potential
Transmembrane231 – 25121Helical; Potential
Transmembrane256 – 27621Helical; Potential
Transmembrane348 – 36821Helical; Potential
Transmembrane376 – 39621Helical; Potential
Transmembrane791 – 81121Helical; Potential
Transmembrane853 – 87321Helical; Potential
Transmembrane934 – 95421Helical; Potential
Transmembrane1031 – 105121Helical; Potential
Domain123 – 403281ABC transmembrane type-1 1
Domain467 – 701235ABC transporter 1
Domain795 – 1082288ABC transmembrane type-1 2
Domain1120 – 1354235ABC transporter 2
Nucleotide binding513 – 5208ATP 1 Potential
Nucleotide binding1154 – 11618ATP 2 Potential

Amino acid modifications

Glycosylation4051N-linked (GlcNAc...) Potential
Glycosylation4381N-linked (GlcNAc...) Potential
Glycosylation5401N-linked (GlcNAc...) Potential
Glycosylation9811N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence142 – 1432VI → TV in isoform 2, isoform 3, isoform 4 and isoform 5.
VSP_021084
Alternative sequence220 – 27758Missing in isoform 5.
VSP_021085
Alternative sequence572 – 63362YQHTV…PLSAV → LGSGASTSLGGRGRGLAWPA LSTPTVSSTCWTTPCRPWTP TWGSTSLRSALRRRSGERQS SW in isoform 4.
VSP_021086
Alternative sequence634 – 1359726Missing in isoform 4.
VSP_021087
Alternative sequence734 – 7363Missing in isoform 2 and isoform 3.
VSP_021088
Alternative sequence737 – 76024Missing in isoform 5.
VSP_021089
Alternative sequence892 – 93847ILKSP…FFMVV → HFPQRSPGAQEGGECQPVTL VHPHHLLHAGPGHHSRLWQE GELHHLV in isoform 3.
VSP_021090
Alternative sequence939 – 1359421Missing in isoform 3.
VSP_021091
Alternative sequence1013 – 1359347Missing in isoform 2 and isoform 5.
VSP_021092
Natural variant91I → L.
Corresponds to variant rs16945901 [ dbSNP | Ensembl ].
VAR_028392
Natural variant1021A → E.
Corresponds to variant rs16945874 [ dbSNP | Ensembl ].
VAR_028393
Natural variant5871N → Y.
Corresponds to variant rs16945816 [ dbSNP | Ensembl ].
VAR_028394
Natural variant6901E → V.
Corresponds to variant rs34135219 [ dbSNP | Ensembl ].
VAR_048139
Natural variant8941K → M.
Corresponds to variant rs8057474 [ dbSNP | Ensembl ].
VAR_028395
Natural variant9891T → S.
Corresponds to variant rs6500305 [ dbSNP | Ensembl ].
VAR_028396
Natural variant10131Y → H.
Corresponds to variant rs6500304 [ dbSNP | Ensembl ].
VAR_028397
Natural variant11171R → C. Ref.2
Corresponds to variant rs7193955 [ dbSNP | Ensembl ].
VAR_028398
Natural variant11871I → T.
Corresponds to variant rs34106426 [ dbSNP | Ensembl ].
VAR_048140
Natural variant11911E → A.
Corresponds to variant rs16945787 [ dbSNP | Ensembl ].
VAR_028399
Natural variant13491F → L.
Corresponds to variant rs12373105 [ dbSNP | Ensembl ].
VAR_028400

Experimental info

Sequence conflict731T → A in BC036378. Ref.5
Sequence conflict448 – 4503SRK → RQE in AAL79528. Ref.1
Sequence conflict448 – 4503SRK → RQE in AAL79529. Ref.1
Sequence conflict448 – 4503SRK → RQE in AAL99900. Ref.1
Sequence conflict448 – 4503SRK → RQE in AAL99901. Ref.1
Sequence conflict8531W → R in AAL79528. Ref.1
Sequence conflict8531W → R in AAL79529. Ref.1
Sequence conflict8531W → R in AAL99900. Ref.1
Sequence conflict8531W → R in AAL99901. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: DA8AF5A3EE9B10E8

FASTA1,359152,297
        10         20         30         40         50         60 
MVGEGPYLIS DLDQRGRRRS FAERYDPSLK TMIPVRPCAR LAPNPVDDAG LLSFATFSWL 

        70         80         90        100        110        120 
TPVMVKGYRQ RLTVDTLPPL STYDSSDTNA KRFRVLWDEE VARVGPEKAS LSHVVWKFQR 

       130        140        150        160        170        180 
TRVLMDIVAN ILCIIMAAIG PVILIHQILQ QTERTSGKVW VGIGLCIALF ATEFTKVFFW 

       190        200        210        220        230        240 
ALAWAINYRT AIRLKVALST LVFENLVSFK TLTHISVGEV LNILSSDSYS LFEAALFCPL 

       250        260        270        280        290        300 
PATIPILMVF CAAYAFFILG PTALIGISVY VIFIPVQMFM AKLNSAFRRS AILVTDKRVQ 

       310        320        330        340        350        360 
TMNEFLTCIR LIKMYAWEKS FTNTIQDIRR RERKLLEKAG FVQSGNSALA PIVSTIAIVL 

       370        380        390        400        410        420 
TLSCHILLRR KLTAPVAFSV IAMFNVMKFS IAILPFSIKA MAEANVSLRR MKKILIDKSP 

       430        440        450        460        470        480 
PSYITQPEDP DTVLLLANAT LTWEHEASRK STPKKLQNQK RHLCKKQRSE AYSERSPPAK 

       490        500        510        520        530        540 
GATGPEEQSD SLKSVLHSIS FVVRKGKILG ICGNVGSGKS SLLAALLGQM QLQKGVVAVN 

       550        560        570        580        590        600 
GTLAYVSQQA WIFHGNVREN ILFGEKYDHQ RYQHTVRVCG LQKDLSNLPY GDLTEIGERG 

       610        620        630        640        650        660 
LNLSGGQRQR ISLARAVYSD RQLYLLDDPL SAVDAHVGKH VFEECIKKTL RGKTVVLVTH 

       670        680        690        700        710        720 
QLQFLESCDE VILLEDGEIC EKGTHKELME ERGRYAKLIH NLRGLQFKDP EHLYNAAMVE 

       730        740        750        760        770        780 
AFKESPAERE EDAGIIVLAP GNEKDEGKES ETGSEFVDTK VPEHQLIQTE SPQEGTVTWK 

       790        800        810        820        830        840 
TYHTYIKASG GYLLSLFTVF LFLLMIGSAA FSNWWLGLWL DKGSRMTCGP QGNRTMCEVG 

       850        860        870        880        890        900 
AVLADIGQHV YQWVYTASMV FMLVFGVTKG FVFTKTTLMA SSSLHDTVFD KILKSPMSFF 

       910        920        930        940        950        960 
DTTPTGRLMN RFSKDMDELD VRLPFHAENF LQQFFMVVFI LVILAAVFPA VLLVVASLAV 

       970        980        990       1000       1010       1020 
GFFILLRIFH RGVQELKKVE NVSRSPWFTH ITSSMQGLGI IHAYGKKESC ITYHLLYFNC 

      1030       1040       1050       1060       1070       1080 
ALRWFALRMD VLMNILTFTV ALLVTLSFSS ISTSSKGLSL SYIIQLSGLL QVCVRTGTET 

      1090       1100       1110       1120       1130       1140 
QAKFTSVELL REYISTCVPE CTHPLKVGTC PKDWPSRGEI TFRDYQMRYR DNTPLVLDSL 

      1150       1160       1170       1180       1190       1200 
NLNIQSGQTV GIVGRTGSGK SSLGMALFRL VEPASGTIFI DEVDICILSL EDLRTKLTVI 

      1210       1220       1230       1240       1250       1260 
PQDPVLFVGT VRYNLDPFES HTDEMLWQVL ERTFMRDTIM KLPEKLQAEV TENGENFSVG 

      1270       1280       1290       1300       1310       1320 
ERQLLCVARA LLRNSKIILL DEATASMDSK TDTLVQNTIK DAFKGCTVLT IAHRLNTVLN 

      1330       1340       1350 
CDHVLVMENG KVIEFDKPEV LAEKPDSAFA MLLAAEVRL 

« Hide

Isoform 2 (A) (C) (D) [UniParc].

Checksum: 6491AA00FBC280E5
Show »

FASTA1,009113,163
Isoform 3 (B) [UniParc].

Checksum: AD0715883A3971E2
Show »

FASTA935104,651
Isoform 4 [UniParc].

Checksum: 66DEE4BFA9C4D7E4
Show »

FASTA63370,399
Isoform 5 [UniParc].

Checksum: 22A26E88E51CE234
Show »

FASTA930104,660

References

« Hide 'large scale' references
[1]"Multiple splicing variants of two new human ATP-binding cassette transporters, ABCC11 and ABCC12."
Yabuuchi H., Shimizu H., Takayanagi S., Ishikawa T.
Biochem. Biophys. Res. Commun. 288:933-939(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Liver, Pancreas and Testis.
[2]"Two new genes from the human ATP-binding cassette transporter superfamily, ABCC11 and ABCC12, tandemly duplicated on chromosome 16q12."
Tammur J., Prades C., Arnould I., Rzhetsky A., Hutchinson A., Adachi M., Schuetz J.D., Swoboda K.J., Ptacek L.J., Rosier M., Dean M., Allikmets R.
Gene 273:89-96(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT CYS-1117, TISSUE SPECIFICITY.
Tissue: Testis.
[3]"MRP9, an unusual truncated member of the ABC transporter superfamily, is highly expressed in breast cancer."
Bera T.K., Iavarone C., Kumar V., Lee S., Lee B., Pastan I.
Proc. Natl. Acad. Sci. U.S.A. 99:6997-7002(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), TISSUE SPECIFICITY.
[4]"The sequence and analysis of duplication-rich human chromosome 16."
Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. expand/collapse author list , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Tissue: Testis.

Web resources

ABCMdb

Database for mutations in ABC proteins

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF395908 mRNA. Translation: AAL79528.1.
AF395909 mRNA. Translation: AAL79529.1.
AF411577 mRNA. Translation: AAL99900.1.
AF411578 mRNA. Translation: AAL99901.1.
AY040220 mRNA. Translation: AAK76740.1.
AY196326 mRNA. Translation: AAO40749.1.
AC096996 Genomic DNA. No translation available.
BC036378 mRNA. No translation available.
RefSeqNP_150229.2. NM_033226.2.
UniGeneHs.410111.

3D structure databases

ProteinModelPortalQ96J65.
SMRQ96J65. Positions 149-729, 779-1345.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125142. 1 interaction.
IntActQ96J65. 1 interaction.
STRING9606.ENSP00000311030.

PTM databases

PhosphoSiteQ96J65.

Polymorphism databases

DMDM161788999.

Proteomic databases

PaxDbQ96J65.
PRIDEQ96J65.

Protocols and materials databases

DNASU94160.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000311303; ENSP00000311030; ENSG00000140798. [Q96J65-1]
ENST00000416054; ENSP00000413046; ENSG00000140798. [Q96J65-4]
ENST00000448542; ENSP00000401855; ENSG00000140798. [Q96J65-3]
ENST00000497206; ENSP00000431232; ENSG00000140798. [Q96J65-2]
ENST00000529084; ENSP00000434510; ENSG00000140798. [Q96J65-4]
ENST00000529504; ENSP00000433333; ENSG00000140798. [Q96J65-3]
ENST00000532494; ENSP00000437047; ENSG00000140798. [Q96J65-2]
ENST00000534418; ENSP00000431354; ENSG00000140798. [Q96J65-5]
GeneID94160.
KEGGhsa:94160.
UCSCuc002efc.1. human. [Q96J65-1]
uc002efe.1. human. [Q96J65-4]

Organism-specific databases

CTD94160.
GeneCardsGC16M048165.
H-InvDBHIX0026945.
HGNCHGNC:14640. ABCC12.
HPAHPA043100.
MIM607041. gene.
neXtProtNX_Q96J65.
PharmGKBPA24394.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1132.
HOVERGENHBG108314.
InParanoidQ96J65.
KOK05672.
OMACVPECTH.
OrthoDBEOG7MWGW0.
PhylomeDBQ96J65.
TreeFamTF105202.

Gene expression databases

ArrayExpressQ96J65.
BgeeQ96J65.
CleanExHS_ABCC12.
GenevestigatorQ96J65.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiABCC12.
GenomeRNAi94160.
NextBio78453.
PROQ96J65.
SOURCESearch...

Entry information

Entry nameMRP9_HUMAN
AccessionPrimary (citable) accession number: Q96J65
Secondary accession number(s): Q49AL2, Q8TAF0, Q8TEY2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: December 4, 2007
Last modified: April 16, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM