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Protein

Kinetochore-associated protein NSL1 homolog

Gene

NSL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.1 Publication

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Chromosome partition, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore-associated protein NSL1 homolog
Gene namesi
Name:NSL1
Synonyms:C1orf48
ORF Names:DC31, DC8, MIS14
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000117697.14
HGNCiHGNC:24548 NSL1
MIMi609174 gene
neXtProtiNX_Q96IY1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi25936
OpenTargetsiENSG00000117697
PharmGKBiPA162398188

Polymorphism and mutation databases

BioMutaiNSL1
DMDMi147744577

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000892601 – 281Kinetochore-associated protein NSL1 homologAdd BLAST281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineCombined sources1
Modified residuei244PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ96IY1
MaxQBiQ96IY1
PaxDbiQ96IY1
PeptideAtlasiQ96IY1
PRIDEiQ96IY1

PTM databases

iPTMnetiQ96IY1
PhosphoSitePlusiQ96IY1

Expressioni

Gene expression databases

BgeeiENSG00000117697
CleanExiHS_NSL1
ExpressionAtlasiQ96IY1 baseline and differential
GenevisibleiQ96IY1 HS

Organism-specific databases

HPAiHPA045761
HPA053721

Interactioni

Subunit structurei

Component of the MIS12 complex composed of MIS12, DSN1, NSL1/DC8 and PMF1. Interacts with KNL1.2 Publications

Protein-protein interaction databases

BioGridi11743334 interactors.
CORUMiQ96IY1
IntActiQ96IY1 19 interactors.
MINTiQ96IY1
STRINGi9606.ENSP00000355944

Structurei

Secondary structure

1281
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi37 – 41Combined sources5
Helixi43 – 58Combined sources16
Helixi59 – 63Combined sources5
Turni66 – 68Combined sources3
Helixi69 – 85Combined sources17
Beta strandi88 – 90Combined sources3
Helixi106 – 149Combined sources44
Beta strandi167 – 169Combined sources3
Helixi170 – 200Combined sources31
Turni267 – 269Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NF9X-ray2.80C/D256-281[»]
5LSIX-ray2.00E29-99[»]
5LSJX-ray3.25G/N92-206[»]
5LSKX-ray3.50N1-206[»]
ProteinModelPortaliQ96IY1
SMRiQ96IY1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG410IXS8 Eukaryota
ENOG41125EG LUCA
GeneTreeiENSGT00390000001374
HOGENOMiHOG000113863
HOVERGENiHBG080948
InParanoidiQ96IY1
KOiK11545
OMAiMQPIIQL
OrthoDBiEOG091G0LWG
PhylomeDBiQ96IY1
TreeFamiTF333388

Family and domain databases

InterProiView protein in InterPro
IPR013950 Mis14/Nsl1
PANTHERiPTHR31749 PTHR31749, 1 hit
PfamiView protein in Pfam
PF08641 Mis14, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96IY1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGSPELVVL DPPWDKELAA GTESQALVSA TPREDFRVRC TSKRAVTEML
60 70 80 90 100
QLCGRFVQKL GDALPEEIRE PALRDAQWTF ESAVQENISI NGQAWQEASD
110 120 130 140 150
NCFMDSDIKV LEDQFDEIIV DIATKRKQYP RKILECVIKT IKAKQEILKQ
160 170 180 190 200
YHPVVHPLDL KYDPDPAPHM ENLKCRGETV AKEISEAMKS LPALIEQGEG
210 220 230 240 250
FSQVLRMQPV IHLQRIHQEV FSSCHRKPDA KPENFITQIE TTPTETASRK
260 270 280
TSDMVLKRKQ TKDCPQRKWY PLRPKKINLD T
Length:281
Mass (Da):32,162
Last modified:May 15, 2007 - v3
Checksum:iF9FB4379BAFF2626
GO
Isoform 2 (identifier: Q96IY1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     167-281: APHMENLKCR...LRPKKINLDT → VLNGNAFNFS...QRGNSSKGDQ

Show »
Length:213
Mass (Da):24,108
Checksum:iD3C6861CF654274C
GO

Sequence cautioni

The sequence AAF86877 differs from that shown. Reason: Frameshift at position 41.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0512434S → F1 PublicationCorresponds to variant dbSNP:rs17856201Ensembl.1
Natural variantiVAR_016005254M → V2 PublicationsCorresponds to variant dbSNP:rs15702Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_045741167 – 281APHME…INLDT → VLNGNAFNFSPFNMMLAVDL SYMVFITSSPSYGKFEMQRG NSSKGDQ in isoform 2. CuratedAdd BLAST115

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF201941 mRNA Translation: AAF86877.1 Frameshift.
AF255793 mRNA Translation: AAG44640.1
AL591647, AC104333 Genomic DNA Translation: CAI17046.1
BC007067 mRNA Translation: AAH07067.1
AL050084 mRNA Translation: CAB43260.1
CCDSiCCDS1509.1 [Q96IY1-1]
CCDS53474.1 [Q96IY1-2]
PIRiT08737
RefSeqiNP_001036014.1, NM_001042549.1 [Q96IY1-2]
NP_056286.3, NM_015471.3 [Q96IY1-1]
UniGeneiHs.497692

Genome annotation databases

EnsembliENST00000366977; ENSP00000355944; ENSG00000117697 [Q96IY1-1]
ENST00000626725; ENSP00000486783; ENSG00000117697 [Q96IY1-2]
GeneIDi25936
KEGGihsa:25936
UCSCiuc001hjn.4 human [Q96IY1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNSL1_HUMAN
AccessioniPrimary (citable) accession number: Q96IY1
Secondary accession number(s): E7ETD5
, Q5SY75, Q9H2M5, Q9NRN8, Q9Y415
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: May 15, 2007
Last modified: February 28, 2018
This is version 135 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome