Q96IU4 (ABHEB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Abhydrolase domain-containing protein 14B
EC=3.-.-.-

Alternative name(s):
CCG1-interacting factor B

Gene names

Name:	ABHD14B
Synonyms:	CIB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has hydrolase activity towards p-nitrophenyl butyrate (in vitro). May activate transcription. Ref.6
Subunit structure	May interact with TAF1.
Subcellular location	Cytoplasm. Nucleus. Note: Predominantly cytoplasmic. Ref.5 Ref.6
Tissue specificity	Ubiquitous. Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. Ref.6
Sequence similarities	Belongs to the AB hydrolase superfamily. ABHD14 family.
Sequence caution	The sequence AAH50650.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase
PTM	Acetylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay. Source: HPA nucleolus Inferred from direct assay. Source: HPA
Molecular function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96IU4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96IU4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: MAASVEQREG...AGYRAVAIDL → MGPGLFPAFLLRPQVTASTRLLPVCASPRSS

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 210

209

Abhydrolase domain-containing protein 14B

PRO_0000065038

Sites

Active site

111

1

Charge relay system By similarity

Active site

162

1

Charge relay system By similarity

Active site

188

1

Charge relay system By similarity

Amino acid modifications

Modified residue

2

1

N-acetylalanine Ref.3

Natural variations

Alternative sequence

1 – 69

69

MAASV…VAIDL → MGPGLFPAFLLRPQVTASTR LLPVCASPRSS in isoform 2.

VSP_008058

Secondary structure

1

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210

Helix Strand Turn

Details...

Beta strand

5 – 7

3

Beta strand

12 – 14

3

Beta strand

17 – 19

3

Beta strand

21 – 25

5

Beta strand

27 – 29

3

Beta strand

34 – 37

4

Helix

45 – 51

7

Helix

53 – 59

7

Beta strand

63 – 67

5

Helix

73 – 75

3

Helix

91 – 100

10

Beta strand

106 – 110

5

Helix

111 – 113

3

Helix

114 – 121

8

Beta strand

129 – 135

7

Helix

139 – 141

3

Helix

144 – 148

5

Beta strand

154 – 159

6

Helix

163 – 172

10

Beta strand

175 – 183

9

Helix

190 – 193

4

Helix

195 – 207

13

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 3CACE8759A2ADFAD	FASTA	210	22,346
	10 20 30 40 50 60 MAASVEQREG TIQVQGQALF FREALPGSGQ ARFSVLLLHG IRFSSETWQN LGTLHRLAQA 70 80 90 100 110 120 GYRAVAIDLP GLGHSKEAAA PAPIGELAPG SFLAAVVDAL ELGPPVVISP SLSGMYSLPF 130 140 150 160 170 180 LTAPGSQLPG FVPVAPICTD KINAANYASV KTPALIVYGD QDPMGQTSFE HLKQLPNHRV 190 200 210 LIMKGAGHPC YLDKPEEWHT GLLDFLQGLQ
	Isoform 2 [UniParc]. Checksum: 4C73568207B54B5C	FASTA	172	18,046
	10 20 30 40 50 60 MGPGLFPAFL LRPQVTASTR LLPVCASPRS SPGLGHSKEA AAPAPIGELA PGSFLAAVVD 70 80 90 100 110 120 ALELGPPVVI SPSLSGMYSL PFLTAPGSQL PGFVPVAPIC TDKINAANYA SVKTPALIVY 130 140 150 160 170 GDQDPMGQTS FEHLKQLPNH RVLIMKGAGH PCYLDKPEEW HTGLLDFLQG LQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Kidney, Ovarian carcinoma and Placenta.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung and Skin.
[3]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[4]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]	"Purification, crystallization and preliminary X-ray crystallographic analysis of human CCG1-interacting factor B." Padmanabhan B., Kuzuhara T., Mizuno H., Horikoshi M. Acta Crystallogr. D 56:1479-1481(2000) [PubMed: 11053859] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS), INTERACTION WITH TAF1, SUBCELLULAR LOCATION.
[6]	"The crystal structure of CCG1/TAF(II)250-interacting factor B (CIB)." Padmanabhan B., Kuzuhara T., Adachi N., Horikoshi M. J. Biol. Chem. 279:9615-9624(2004) [PubMed: 14672934] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS), FUNCTION, ACTIVE SITE, SUBCELLULAR LOCATION, INTERACTION WITH TAF1, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK075034 mRNA. Translation: BAC11366.1.
AK075112 mRNA. Translation: BAC11408.1.
AK096073 mRNA. Translation: BAC04696.1.
BC007234 mRNA. Translation: AAH07234.1.
BC050650 mRNA. Translation: AAH50650.1. Different initiation.
BC056411 mRNA. Translation: AAH56411.1.

IPI

IPI00063827.
IPI00167615.

RefSeq

NP_001139786.1. NM_001146314.1.
NP_116139.1. NM_032750.2.

UniGene

Hs.420796.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IMJ	X-ray	2.20	A	1-210	[»]

ProteinModelPortal

Q96IU4.

SMR

Q96IU4. Positions 2-209.

ModBase

Search...

Protein-protein interaction databases

STRING

Q96IU4.

Protein family/group databases

MEROPS

S33.983.

Polymorphism databases

DMDM

34222621.

2D gel databases

SWISS-2DPAGE

Q96IU4.

OGP

Q96IU4.

REPRODUCTION-2DPAGE

IPI00063827.

Proteomic databases

PeptideAtlas

Q96IU4.

PRIDE

Q96IU4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000361143; ENSP00000354841; ENSG00000114779.
ENST00000395008; ENSP00000378455; ENSG00000114779.
ENST00000483233; ENSP00000420065; ENSG00000114779.

GeneID

84836.

KEGG

hsa:84836.

UCSC

uc003dcm.1. human.

Organism-specific databases

CTD

84836.

GeneCards

GC03M052003.

H-InvDB

HIX0003339.

HGNC

HGNC:28235. ABHD14B.

HPA

HPA036444.
HPA036642.

neXtProt

NX_Q96IU4.

PharmGKB

PA142672660.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG14496.

GeneTree

ENSGT00510000046860.

HOGENOM

HBG727630.

HOVERGEN

HBG001936.

InParanoid

Q96IU4.

OMA

QNLGTLH.

OrthoDB

EOG444KMC.

PhylomeDB

Q96IU4.

Gene expression databases

ArrayExpress

Q96IU4.

Bgee

Q96IU4.

CleanEx

HS_ABHD14B.

Genevestigator

Q96IU4.

GermOnline

ENSG00000114779. Homo sapiens.

Family and domain databases

KO

K13706.

ProtoNet

Search...

Other

NextBio

75053.

Entry information

Entry name

ABHEB_HUMAN

Accession

Primary (citable) accession number: Q96IU4
Secondary accession number(s): Q86VK8, Q8N8W5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 22, 2003
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.