Q96IC2 (REXON_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative RNA exonuclease NEF-sp
EC=3.1.-.-

Gene names

ORF Names:

44M2.3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	774 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Sequence similarities	Contains 1 exonuclease domain. Contains 2 RRM (RNA recognition motif) domains.
Sequence caution	The sequence AAC31668.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	RNA-binding
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	nucleolus Inferred from direct assay. Source: LIFEdb
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96IC2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96IC2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     543-573: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 774	774	Putative RNA exonuclease NEF-sp		PRO_0000287345
Regions
	Domain	228 – 376	149	Exonuclease
	Domain	505 – 579	75	RRM 1
	Domain	600 – 679	80	RRM 2
Natural variations
	Alternative sequence	543 – 573	31	Missing in isoform 2.		VSP_025443
Experimental info
	Sequence conflict	18	1	S → G in AAK17192. Ref.1
	Sequence conflict	213	1	F → S in AK057254. Ref.3
	Sequence conflict	748	1	S → R in AAC31668. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 3860D67555EDF91F	FASTA	774	86,886
	10 20 30 40 50 60 MEPEREGTER HPRKVRESRQ APNKLVGAAE AMKAGWDLEE SQPEAKKARL STILFTDNCE 70 80 90 100 110 120 VTHDQLCELL KYAVLGKSNV PKPSWCQLFH QNHLNNVVVF VLQGMSQLHF YRFYLEFGCL 130 140 150 160 170 180 RKAFRHKFRL PPPSSDFLAD VVGLQTEQRA GDLPKTMEGP LPSNAKAAIN LQDDPIIQKY 190 200 210 220 230 240 GSKKVGLTRC LLTKEEMRTF HFPLQGFPDC ENFLLTKCNG SIADNSPLFG LDCEMCLTSK 250 260 270 280 290 300 GRELTRISLV AEGGCCVMDE LVKPENKILD YLTSFSGITK KILNPVTTKL KDVQRQLKAL 310 320 330 340 350 360 LPPDAVLVGH SLDLDLRALK MIHPYVIDTS LLYVREQGRR FKLKFLAKVI LGKDIQCPDR 370 380 390 400 410 420 LGHDATEDAR TILELARYFL KHGPKKIAEL NLEALANHQE IQAAGQEPKN TAEVLQHPNT 430 440 450 460 470 480 SVLECLDSVG QKLLFLTRET DAGELPSSRN CQTIKCLSNK EVLEQARVEI PLFPFSIVQF 490 500 510 520 530 540 SFKAFSPVLT EEMNKRMRIK WTEISTVYAG PFSKNCNLRA LKRLFKSFGP VQSMTFVLET 550 560 570 580 590 600 RQPHLCIQYE VLEAAQLAIE SLDGILVDGI CIKVQRPVTE LTLDCDTLVN ELEGDSENQG 610 620 630 640 650 660 SIYLSGVSET FKEQLLQEPR LFLGLEAVIL PKDLKSGKQK KYCFLKFKSF GSAQQALNIL 670 680 690 700 710 720 TGKDWKLKGR HALTPRHLHA WLRGLPPEST RLPGLRVVPP PFEQEALQTL KLDHPKIAAW 730 740 750 760 770 RWSRKIGKLY NSLCPGTLCL ILLPGTKSTH GSLSGLGLMG IKEEEESAGP GLCS
	Isoform 2 [UniParc]. Checksum: B8DB32F3E54A1E3B	FASTA	743	83,536
	10 20 30 40 50 60 MEPEREGTER HPRKVRESRQ APNKLVGAAE AMKAGWDLEE SQPEAKKARL STILFTDNCE 70 80 90 100 110 120 VTHDQLCELL KYAVLGKSNV PKPSWCQLFH QNHLNNVVVF VLQGMSQLHF YRFYLEFGCL 130 140 150 160 170 180 RKAFRHKFRL PPPSSDFLAD VVGLQTEQRA GDLPKTMEGP LPSNAKAAIN LQDDPIIQKY 190 200 210 220 230 240 GSKKVGLTRC LLTKEEMRTF HFPLQGFPDC ENFLLTKCNG SIADNSPLFG LDCEMCLTSK 250 260 270 280 290 300 GRELTRISLV AEGGCCVMDE LVKPENKILD YLTSFSGITK KILNPVTTKL KDVQRQLKAL 310 320 330 340 350 360 LPPDAVLVGH SLDLDLRALK MIHPYVIDTS LLYVREQGRR FKLKFLAKVI LGKDIQCPDR 370 380 390 400 410 420 LGHDATEDAR TILELARYFL KHGPKKIAEL NLEALANHQE IQAAGQEPKN TAEVLQHPNT 430 440 450 460 470 480 SVLECLDSVG QKLLFLTRET DAGELPSSRN CQTIKCLSNK EVLEQARVEI PLFPFSIVQF 490 500 510 520 530 540 SFKAFSPVLT EEMNKRMRIK WTEISTVYAG PFSKNCNLRA LKRLFKSFGP VQSMTFVLET 550 560 570 580 590 600 RQVQRPVTEL TLDCDTLVNE LEGDSENQGS IYLSGVSETF KEQLLQEPRL FLGLEAVILP 610 620 630 640 650 660 KDLKSGKQKK YCFLKFKSFG SAQQALNILT GKDWKLKGRH ALTPRHLHAW LRGLPPESTR 670 680 690 700 710 720 LPGLRVVPPP FEQEALQTLK LDHPKIAAWR WSRKIGKLYN SLCPGTLCLI LLPGTKSTHG 730 740 SLSGLGLMGI KEEEESAGPG LCS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a novel exonuclease factor gene related to testis development in humans." Li J.M., Sha J.H., Zhou Z.M. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Testis.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[4]	"Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q." Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R., Fuhrmann J., Mason T., Crosby M.L., Barnstead M., Cronin L., Mays A.D., Cao Y., Xu R.X., Kang H.-L., Mitchell S., Eichler E.E., Harris P.C., Venter J.C., Adams M.D. Genomics 60:295-308(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung and Testis.
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 416-774 (ISOFORM 1). Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF332193 mRNA. Translation: AAK17192.1. AL136763 mRNA. Translation: CAB66697.1. AK057254 mRNA. No translation available. AK314458 mRNA. Translation: BAG37066.1. AC004381 Genomic DNA. Translation: AAC31668.1. Sequence problems. CH471228 Genomic DNA. Translation: EAW66838.1. BC007646 mRNA. Translation: AAH07646.1. BC068503 mRNA. Translation: AAH68503.1. AL162035 mRNA. Translation: CAB82386.1.
IPI	IPI00021754. IPI00307029.
PIR	T47187.
RefSeq	NP_001138396.1. NM_001144924.1. NP_001185982.1. NM_001199053.1. NP_112203.2. NM_030941.2.
UniGene	Hs.177926.
3D structure databases
ProteinModelPortal	Q96IC2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96IC2. 1 interaction.
MINT	MINT-4720634.
PTM databases
PhosphoSite	Q96IC2.
Polymorphism databases
DMDM	74760869.
Proteomic databases
PaxDb	Q96IC2.
PRIDE	Q96IC2.
Protocols and materials databases
DNASU	81691.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	81691.
KEGG	hsa:81691.
UCSC	uc002dhv.3. human. uc002dhx.3. human.
Organism-specific databases
GeneCards	GC16P020817.
HPA	HPA040755. HPA040868.
neXtProt	NX_Q96IC2.
Phylogenomic databases
eggNOG	COG0847.
HOVERGEN	HBG101031.
InParanoid	Q96IC2.
KO	K14570.
OMA	KMIHPYV.
OrthoDB	EOG44F69Z.
PhylomeDB	Q96IC2.
Gene expression databases
ArrayExpress	Q96IC2.
Bgee	Q96IC2.
Genevestigator	Q96IC2.
Family and domain databases
Gene3D	3.30.70.330. 2 hits.
InterPro	IPR006055. Exonuclease. IPR013520. Exonuclease_RNaseT/DNA_pol3. IPR012677. Nucleotide-bd_a/b_plait. IPR012337. RNaseH-like_dom. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00929. RNase_T. 1 hit. PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00479. EXOIII. 1 hit. SM00360. RRM. 2 hits. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
PROSITE	PS50102. RRM. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	81691.
NextBio	72040.

Entry information

Entry name

REXON_HUMAN

Accession

Primary (citable) accession number: Q96IC2
Secondary accession number(s): B2RB19 , O60364, Q96MB1, Q9BXH9, Q9H0K2, Q9NSM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	December 1, 2001
Last modified:	May 29, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.