Q96I99 (SUCB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial
EC=6.2.1.4

Alternative name(s):
GTP-specific succinyl-CoA synthetase subunit beta
Succinyl-CoA synthetase beta-G chain
Short name=SCS-betaG

Gene names

Name:

SUCLG2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the GTP-dependent ligation of succinate and CoA to form succinyl-CoA By similarity.
Catalytic activity	GTP + succinate + CoA = GDP + phosphate + succinyl-CoA.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Heterodimer of an alpha and a beta subunit.
Subcellular location	Mitochondrion.
Tissue specificity	Mainly expressed in liver, kidney, heart, spleen and skeletal muscle. Also found in intestine and colon, and in low amounts in lung, brain, prostate, testis and ovary. Ref.4
Sequence similarities	Belongs to the succinate/malate CoA ligase beta subunit family. Contains 1 ATP-grasp domain.
Sequence caution	The sequence AAH07716.3 differs from that shown. Reason: Erroneous initiation. The sequence AAH47024.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAH47024.1 differs from that shown. Reason: Erroneous termination at position 31. Translated as Gln.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transit peptide
Ligand	GTP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	small molecule metabolic process Traceable author statement. Source: Reactome succinyl-CoA metabolic process Non-traceable author statement Ref.4. Source: UniProtKB tricarboxylic acid cycle Traceable author statement. Source: Reactome
Cellular_component	mitochondrial matrix Traceable author statement. Source: Reactome
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro GTP binding Inferred from electronic annotation. Source: UniProtKB-KW succinate-CoA ligase (GDP-forming) activity Non-traceable author statement Ref.4. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96I99-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96I99-2)

The sequence of this isoform differs from the canonical sequence as follows:
     397-432: NVQEAQKILN...KKAVASVAKK → FMEKKGSYMH...SKCAISIFLC

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 37	37	Mitochondrion By similarity
	Chain	38 – 432	395	Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial		PRO_0000033356
Regions
	Domain	46 – 274	229	ATP-grasp
Amino acid modifications
	Modified residue	227	1	N6-acetyllysine Ref.6
	Modified residue	291	1	N6-acetyllysine Ref.6
Natural variations
	Alternative sequence	397 – 432	36	NVQEA…SVAKK → FMEKKGSYMHIKQETGNSNE NITGIQENVAHQNLSKCAIS IFLC in isoform 2.		VSP_042013
	Natural variant	347	1	K → R. Corresponds to variant rs9843840 [ dbSNP | Ensembl ].		VAR_052499
	Natural variant	381	1	R → W. Corresponds to variant rs7623258 [ dbSNP | Ensembl ].		VAR_052500
Experimental info
	Sequence conflict	174 – 175	2	GV → RS in AAC64397. Ref.4
	Sequence conflict	220	1	Q → K in AAH68602. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 27, 2004. Version 2. Checksum: 56A977A3E50713A1	FASTA	432	46,511
	10 20 30 40 50 60 MASPVAAQAG KLLRALALRP RFLAAGSQAV QLTSRRWLNL QEYQSKKLMS DNGVRVQRFF 70 80 90 100 110 120 VADTANEALE AAKRLNAKEI VLKAQILAGG RGKGVFNSGL KGGVHLTKDP NVVGQLAKQM 130 140 150 160 170 180 IGYNLATKQT PKEGVKVNKV MVAEALDISR ETYLAILMDR SCNGPVLVGS PQGGVDIEEV 190 200 210 220 230 240 AASNPELIFK EQIDIFEGIK DSQAQRMAEN LGFVGPLKSQ AADQITKLYN LFLKIDATQV 250 260 270 280 290 300 EVNPFGETPE GQVVCFDAKI NFDDNAEFRQ KDIFAMDDKS ENEPIENEAA KYDLKYIGLD 310 320 330 340 350 360 GNIACFVNGA GLAMATCDII FLNGGKPANF LDLGGGVKEA QVYQAFKLLT ADPKVEAILV 370 380 390 400 410 420 NIFGGIVNCA IIANGITKAC RELELKVPLV VRLEGTNVQE AQKILNNSGL PITSAIDLED 430 AAKKAVASVA KK
	Isoform 2 [UniParc]. Checksum: FFBA486CDA19334B	FASTA	440	47,732
	10 20 30 40 50 60 MASPVAAQAG KLLRALALRP RFLAAGSQAV QLTSRRWLNL QEYQSKKLMS DNGVRVQRFF 70 80 90 100 110 120 VADTANEALE AAKRLNAKEI VLKAQILAGG RGKGVFNSGL KGGVHLTKDP NVVGQLAKQM 130 140 150 160 170 180 IGYNLATKQT PKEGVKVNKV MVAEALDISR ETYLAILMDR SCNGPVLVGS PQGGVDIEEV 190 200 210 220 230 240 AASNPELIFK EQIDIFEGIK DSQAQRMAEN LGFVGPLKSQ AADQITKLYN LFLKIDATQV 250 260 270 280 290 300 EVNPFGETPE GQVVCFDAKI NFDDNAEFRQ KDIFAMDDKS ENEPIENEAA KYDLKYIGLD 310 320 330 340 350 360 GNIACFVNGA GLAMATCDII FLNGGKPANF LDLGGGVKEA QVYQAFKLLT ADPKVEAILV 370 380 390 400 410 420 NIFGGIVNCA IIANGITKAC RELELKVPLV VRLEGTFMEK KGSYMHIKQE TGNSNENITG 430 440 IQENVAHQNL SKCAISIFLC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[2]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Hippocampus, Mammary gland, Placenta and Uterus.
[4]	"Genetic evidence for the expression of ATP- and GTP-specific succinyl-CoA synthetases in multicellular eucaryotes." Johnson J.D., Mehus J.G., Tews K., Milavetz B.I., Lambeth D.O. J. Biol. Chem. 273:27580-27586(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-432 (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Liver.
[5]	Mei G., Yu W., Gibbs R.A. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 234-432 (ISOFORM 1). Tissue: Brain.
[6]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-227 AND LYS-291, MASS SPECTROMETRY.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK310527 mRNA. No translation available. AC099783 Genomic DNA. No translation available. AC112213 Genomic DNA. No translation available. AC113169 Genomic DNA. No translation available. AC114401 Genomic DNA. No translation available. BC007716 mRNA. Translation: AAH07716.3. Different initiation. BC019868 mRNA. Translation: AAH19868.1. BC047024 mRNA. Translation: AAH47024.1. Sequence problems. BC068602 mRNA. Translation: AAH68602.1. AF058954 mRNA. Translation: AAC64397.1. AF131748 mRNA. Translation: AAD20032.1.
IPI	IPI00096066.
RefSeq	NP_001171070.1. NM_001177599.1. NP_003839.2. NM_003848.3.
UniGene	Hs.644919.
3D structure databases
ProteinModelPortal	Q96I99.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96I99. 4 interactions.
STRING	9606.ENSP00000307432.
PTM databases
PhosphoSite	Q96I99.
Polymorphism databases
DMDM	52788292.
2D gel databases
REPRODUCTION-2DPAGE	IPI00096066.
Proteomic databases
PaxDb	Q96I99.
PRIDE	Q96I99.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000307227; ENSP00000307432; ENSG00000172340. ENST00000493112; ENSP00000419325; ENSG00000172340.
GeneID	8801.
KEGG	hsa:8801.
UCSC	uc003dna.4. human.
Organism-specific databases
CTD	8801.
GeneCards	GC03M067507.
HGNC	HGNC:11450. SUCLG2.
HPA	HPA046705. HPA051998.
MIM	603922. gene.
neXtProt	NX_Q96I99.
PharmGKB	PA36247.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0045.
HOGENOM	HOG000007059.
HOVERGEN	HBG055555.
InParanoid	Q96I99.
KO	K01900.
OMA	TDRATQK.
OrthoDB	EOG4RXZ08.
PhylomeDB	Q96I99.
Enzyme and pathway databases
BioCyc	MetaCyc:HS10493-MONOMER.
Reactome	REACT_111217. Metabolism.
UniPathway	UPA00223.
Gene expression databases
ArrayExpress	Q96I99.
Bgee	Q96I99.
CleanEx	HS_SUCLG2.
Genevestigator	Q96I99.
GermOnline	ENSG00000172340. Homo sapiens.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.261. 1 hit.
InterPro	IPR013650. ATP-grasp_succ-CoA_synth-type. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR005811. CoA_ligase. IPR017866. Succ-CoA_synthase_bsu_CS. IPR005809. Succ_CoA_synthase_bsu. IPR016102. Succinyl-CoA_synth-like. [Graphical view]
PANTHER	PTHR11815. PTHR11815. 1 hit.
Pfam	PF08442. ATP-grasp_2. 1 hit. PF00549. Ligase_CoA. 1 hit. [Graphical view]
PIRSF	PIRSF001554. SucCS_beta. 1 hit.
SUPFAM	SSF52210. CoA_ligase. 1 hit.
TIGRFAMs	TIGR01016. sucCoAbeta. 1 hit.
PROSITE	PS50975. ATP_GRASP. False negative. PS01217. SUCCINYL_COA_LIG_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SUCLG2. human.
DrugBank	DB00139. Succinic acid.
GenomeRNAi	8801.
NextBio	33012.
SOURCE	Search...

Entry information

Entry name

SUCB2_HUMAN

Accession

Primary (citable) accession number: Q96I99
Secondary accession number(s): C9JVT2 , O95195, Q6NUH7, Q86VX8, Q8WUQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	September 27, 2004
Last modified:	May 1, 2013
This is version 125 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.