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Q96I99 (SUCB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial

EC=6.2.1.4
Alternative name(s):
GTP-specific succinyl-CoA synthetase subunit beta
Succinyl-CoA synthetase beta-G chain
Short name=SCS-betaG
Gene names
Name:SUCLG2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the GTP-dependent ligation of succinate and CoA to form succinyl-CoA By similarity.

Catalytic activity

GTP + succinate + CoA = GDP + phosphate + succinyl-CoA.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle.

Subunit structure

Heterodimer of an alpha and a beta subunit.

Subcellular location

Mitochondrion.

Tissue specificity

Mainly expressed in liver, kidney, heart, spleen and skeletal muscle. Also found in intestine and colon, and in low amounts in lung, brain, prostate, testis and ovary. Ref.4

Sequence similarities

Belongs to the succinate/malate CoA ligase beta subunit family.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence AAH07716.3 differs from that shown. Reason: Erroneous initiation.

The sequence AAH47024.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence AAH47024.1 differs from that shown. Reason: Erroneous termination at position 31. Translated as Gln.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96I99-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96I99-2)

The sequence of this isoform differs from the canonical sequence as follows:
     397-432: NVQEAQKILN...KKAVASVAKK → FMEKKGSYMH...SKCAISIFLC
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3737Mitochondrion By similarity
Chain38 – 432395Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial
PRO_0000033356

Regions

Domain46 – 274229ATP-grasp

Amino acid modifications

Modified residue731N6-acetyllysine By similarity
Modified residue781N6-succinyllysine By similarity
Modified residue1321N6-acetyllysine By similarity
Modified residue1391N6-acetyllysine By similarity
Modified residue2001N6-acetyllysine By similarity
Modified residue2181N6-acetyllysine By similarity
Modified residue2271N6-acetyllysine Ref.6
Modified residue2711N6-acetyllysine By similarity
Modified residue2911N6-acetyllysine Ref.6
Modified residue3381N6-succinyllysine By similarity
Modified residue3471N6-acetyllysine By similarity
Modified residue3861N6-acetyllysine By similarity
Modified residue4231N6-acetyllysine By similarity

Natural variations

Alternative sequence397 – 43236NVQEA…SVAKK → FMEKKGSYMHIKQETGNSNE NITGIQENVAHQNLSKCAIS IFLC in isoform 2.
VSP_042013
Natural variant3471K → R.
Corresponds to variant rs9843840 [ dbSNP | Ensembl ].
VAR_052499
Natural variant3811R → W.
Corresponds to variant rs7623258 [ dbSNP | Ensembl ].
VAR_052500

Experimental info

Sequence conflict174 – 1752GV → RS in AAC64397. Ref.4
Sequence conflict2201Q → K in AAH68602. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 27, 2004. Version 2.
Checksum: 56A977A3E50713A1

FASTA43246,511
        10         20         30         40         50         60 
MASPVAAQAG KLLRALALRP RFLAAGSQAV QLTSRRWLNL QEYQSKKLMS DNGVRVQRFF 

        70         80         90        100        110        120 
VADTANEALE AAKRLNAKEI VLKAQILAGG RGKGVFNSGL KGGVHLTKDP NVVGQLAKQM 

       130        140        150        160        170        180 
IGYNLATKQT PKEGVKVNKV MVAEALDISR ETYLAILMDR SCNGPVLVGS PQGGVDIEEV 

       190        200        210        220        230        240 
AASNPELIFK EQIDIFEGIK DSQAQRMAEN LGFVGPLKSQ AADQITKLYN LFLKIDATQV 

       250        260        270        280        290        300 
EVNPFGETPE GQVVCFDAKI NFDDNAEFRQ KDIFAMDDKS ENEPIENEAA KYDLKYIGLD 

       310        320        330        340        350        360 
GNIACFVNGA GLAMATCDII FLNGGKPANF LDLGGGVKEA QVYQAFKLLT ADPKVEAILV 

       370        380        390        400        410        420 
NIFGGIVNCA IIANGITKAC RELELKVPLV VRLEGTNVQE AQKILNNSGL PITSAIDLED 

       430 
AAKKAVASVA KK 

« Hide

Isoform 2 [UniParc].

Checksum: FFBA486CDA19334B
Show »

FASTA44047,732

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Testis.
[2]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Hippocampus, Mammary gland, Placenta and Uterus.
[4]"Genetic evidence for the expression of ATP- and GTP-specific succinyl-CoA synthetases in multicellular eucaryotes."
Johnson J.D., Mehus J.G., Tews K., Milavetz B.I., Lambeth D.O.
J. Biol. Chem. 273:27580-27586(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-432 (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Liver.
[5]Mei G., Yu W., Gibbs R.A.
Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 234-432 (ISOFORM 1).
Tissue: Brain.
[6]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-227 AND LYS-291, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK310527 mRNA. No translation available.
AC099783 Genomic DNA. No translation available.
AC112213 Genomic DNA. No translation available.
AC113169 Genomic DNA. No translation available.
AC114401 Genomic DNA. No translation available.
BC007716 mRNA. Translation: AAH07716.3. Different initiation.
BC019868 mRNA. Translation: AAH19868.1.
BC047024 mRNA. Translation: AAH47024.1. Sequence problems.
BC068602 mRNA. Translation: AAH68602.1.
AF058954 mRNA. Translation: AAC64397.1.
AF131748 mRNA. Translation: AAD20032.1.
CCDSCCDS43104.1. [Q96I99-1]
CCDS54605.1. [Q96I99-2]
RefSeqNP_001171070.1. NM_001177599.1. [Q96I99-2]
NP_003839.2. NM_003848.3. [Q96I99-1]
UniGeneHs.644919.

3D structure databases

ProteinModelPortalQ96I99.
SMRQ96I99. Positions 38-429.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114329. 20 interactions.
IntActQ96I99. 4 interactions.
STRING9606.ENSP00000307432.

Chemistry

DrugBankDB00139. Succinic acid.

PTM databases

PhosphoSiteQ96I99.

Polymorphism databases

DMDM52788292.

2D gel databases

REPRODUCTION-2DPAGEIPI00096066.

Proteomic databases

MaxQBQ96I99.
PaxDbQ96I99.
PRIDEQ96I99.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000307227; ENSP00000307432; ENSG00000172340. [Q96I99-1]
ENST00000493112; ENSP00000419325; ENSG00000172340. [Q96I99-2]
GeneID8801.
KEGGhsa:8801.
UCSCuc003dna.4. human. [Q96I99-1]
uc021xae.1. human. [Q96I99-2]

Organism-specific databases

CTD8801.
GeneCardsGC03M067507.
HGNCHGNC:11450. SUCLG2.
HPAHPA046705.
HPA051998.
MIM603922. gene.
neXtProtNX_Q96I99.
PharmGKBPA36247.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0045.
HOGENOMHOG000007059.
HOVERGENHBG055555.
InParanoidQ96I99.
KOK01900.
OMAEQMIGYN.
OrthoDBEOG7CZK66.
PhylomeDBQ96I99.
TreeFamTF300624.

Enzyme and pathway databases

BioCycMetaCyc:HS10493-MONOMER.
ReactomeREACT_111217. Metabolism.
UniPathwayUPA00223.

Gene expression databases

ArrayExpressQ96I99.
BgeeQ96I99.
CleanExHS_SUCLG2.
GenevestigatorQ96I99.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.261. 1 hit.
InterProIPR013650. ATP-grasp_succ-CoA_synth-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005811. CoA_ligase.
IPR017866. Succ-CoA_synthase_bsu_CS.
IPR005809. Succ_CoA_synthase_bsu.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PANTHERPTHR11815. PTHR11815. 1 hit.
PfamPF08442. ATP-grasp_2. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFPIRSF001554. SucCS_beta. 1 hit.
SUPFAMSSF52210. SSF52210. 1 hit.
TIGRFAMsTIGR01016. sucCoAbeta. 1 hit.
PROSITEPS01217. SUCCINYL_COA_LIG_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSUCLG2. human.
GenomeRNAi8801.
NextBio33012.
PROQ96I99.
SOURCESearch...

Entry information

Entry nameSUCB2_HUMAN
AccessionPrimary (citable) accession number: Q96I99
Secondary accession number(s): C9JVT2 expand/collapse secondary AC list , O95195, Q6NUH7, Q86VX8, Q8WUQ1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: September 27, 2004
Last modified: July 9, 2014
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM