Reviewed, UniProtKB/Swiss-Prot Q96I59 (SYNM_HUMAN)

Names and origin

Protein names

Recommended name:
    Probable asparaginyl-tRNA synthetase, mitochondrial
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:

NARS2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Coding sequence diversity	Polymorphism
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro aspartyl-tRNA aminoacylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from sequence or structural similarity. Source: UniProtKB aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 14	14	Mitochondrion Potential
	Chain	15 – 477	463	Probable asparaginyl-tRNA synthetase, mitochondrial		PRO_0000250722
Amino acid modifications
	Modified residue	353	1	N6-acetyllysine Ref.4
Natural variations
	Natural variant	87	1	T → N: dbSNP rs10501429.		VAR_052636

Sequences

	Sequence		Length	Mass (Da)
	Q96I59-1 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: B2841FB3078E8865	FASTA	477	54,077
	10 20 30 40 50 60 MLGVRCLLRS VRFCSSAPFP KHKPSAKLSV RDALGAQNAS GERIKIQGWI RSVRSQKEVL 70 80 90 100 110 120 FLHVNDGSSL ESLQVVADSG LDSRELTFGS SVEVQGQLIK SPSKRQNVEL KAEKIKVIGN 130 140 150 160 170 180 CDAKDFPIKY KERHPLEYLR QYPHFRCRTN VLGSILRIRS EATAAIHSFF KDSGFVHIHT 190 200 210 220 230 240 PIITSNDSEG AGELFQLEPS GKLKVPEENF FNVPAFLTVS GQLHLEVMSG AFTQVFTFGP 250 260 270 280 290 300 TFRAENSQSR RHLAEFYMIE AEISFVDSLQ DLMQVIEELF KATTMMVLSK CPEDVELCHK 310 320 330 340 350 360 FIAPGQKDRL EHMLKNNFLI ISYTEAVEIL KQASQNFTFT PEWGADLRTE HEKYLVKHCG 370 380 390 400 410 420 NIPVFVINYP LTLKPFYMRD NEDGPQHTVA AVDLLVPGVG ELFGGGLREE RYHFLEERLA 430 440 450 460 470 RSGLTEVYQW YLDLRRFGSV PHGGFGMGFE RYLQCILGVD NIKDVIPFPR FPHSCLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS." Bonnefond L., Fender A., Rudinger-Thirion J., Giege R., Florentz C., Sissler M. Biochemistry 44:4805-4816(2005) [PubMed: 15779907] [Abstract] Cited for: IDENTIFICATION.
[3]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[4]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-353, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC007800 mRNA. Translation: AAH07800.2.
IPI	IPI00101664.
RefSeq	NP_078954.4.
UniGene	Hs.503389
3D structure databases
HSSP	HSSP built from PDB template 1N9W based on UniProtKB Q9LCY8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q96I59.
Proteomic databases
PRIDE	Q96I59.
Genome annotation databases
Ensembl	ENST00000281038; ENSP00000281038; ENSG00000137513; Homo sapiens. [Genome view]
GeneID	79731.
KEGG	hsa:79731.
UCSC	uc001ozi.1. human.
Organism-specific databases
GeneCards	GC11M077825.
HGNC	HGNC:26274. NARS2.
HPA	HPA026793.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q96I59.
Enzyme and pathway databases
BRENDA	6.1.1.22. 247.
Reactome	REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q96I59.
Bgee	Q96I59.
CleanEx	HS_NARS2.
Genevestigator	Q96I59.
GermOnline	ENSG00000137513. Homo sapiens.
Family and domain databases
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00174. L-Asparagine.
NextBio	69114.

Entry information

Entry name

SYNM_HUMAN

Accession

Primary (citable) accession number: Q96I59

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	March 1, 2004
Last modified:	October 13, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.