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Q96I59

- SYNM_HUMAN

UniProt

Q96I59 - SYNM_HUMAN

Protein

Probable asparagine--tRNA ligase, mitochondrial

Gene

NARS2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 3 (18 May 2010)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).

    GO - Molecular functioni

    1. asparagine-tRNA ligase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. asparaginyl-tRNA aminoacylation Source: UniProtKB
    2. gene expression Source: Reactome
    3. tRNA aminoacylation for protein translation Source: Reactome

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_15302. Mitochondrial tRNA aminoacylation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable asparagine--tRNA ligase, mitochondrial (EC:6.1.1.22)
    Alternative name(s):
    Asparaginyl-tRNA synthetase
    Short name:
    AsnRS
    Gene namesi
    Name:NARS2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:26274. NARS2.

    Subcellular locationi

    Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA143485554.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1414MitochondrionSequence AnalysisAdd
    BLAST
    Chaini15 – 477463Probable asparagine--tRNA ligase, mitochondrialPRO_0000250722Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei353 – 3531N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ96I59.
    PaxDbiQ96I59.
    PRIDEiQ96I59.

    PTM databases

    PhosphoSiteiQ96I59.

    Expressioni

    Gene expression databases

    ArrayExpressiQ96I59.
    BgeeiQ96I59.
    CleanExiHS_NARS2.
    GenevestigatoriQ96I59.

    Organism-specific databases

    HPAiHPA026793.

    Interactioni

    Protein-protein interaction databases

    BioGridi122846. 2 interactions.
    IntActiQ96I59. 1 interaction.
    STRINGi9606.ENSP00000281038.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96I59.
    SMRiQ96I59. Positions 41-472.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0017.
    HOGENOMiHOG000226033.
    HOVERGENiHBG067799.
    InParanoidiQ96I59.
    KOiK01893.
    OMAiLEDDMNC.
    OrthoDBiEOG7HF1J5.
    PhylomeDBiQ96I59.
    TreeFamiTF315088.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    HAMAPiMF_00534. Asn_tRNA_synth.
    InterProiIPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004522. Asn-tRNA-ligase.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view]
    PANTHERiPTHR22594. PTHR22594. 1 hit.
    PTHR22594:SF6. PTHR22594:SF6. 1 hit.
    PfamiPF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view]
    PRINTSiPR01042. TRNASYNTHASP.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00457. asnS. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q96I59-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLGVRCLLRS VRFCSSAPFP KHKPSAKLSV RDALGAQNAS GERIKIQGWI    50
    RSVRSQKEVL FLHVNDGSSL ESLQVVADSG LDSRELNFGS SVEVQGQLIK 100
    SPSKRQNVEL KAEKIKVIGN CDAKDFPIKY KERHPLEYLR QYPHFRCRTN 150
    VLGSILRIRS EATAAIHSFF KDSGFVHIHT PIITSNDSEG AGELFQLEPS 200
    GKLKVPEENF FNVPAFLTVS GQLHLEVMSG AFTQVFTFGP TFRAENSQSR 250
    RHLAEFYMIE AEISFVDSLQ DLMQVIEELF KATTMMVLSK CPEDVELCHK 300
    FIAPGQKDRL EHMLKNNFLI ISYTEAVEIL KQASQNFTFT PEWGADLRTE 350
    HEKYLVKHCG NIPVFVINYP LTLKPFYMRD NEDGPQHTVA AVDLLVPGVG 400
    ELFGGGLREE RYHFLEERLA RSGLTEVYQW YLDLRRFGSV PHGGFGMGFE 450
    RYLQCILGVD NIKDVIPFPR FPHSCLL 477
    Length:477
    Mass (Da):54,090
    Last modified:May 18, 2010 - v3
    Checksum:i1F4C78E0B6F5500C
    GO
    Isoform 2 (identifier: Q96I59-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-227: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:250
    Mass (Da):28,783
    Checksum:iDF015513014C4FA9
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti87 – 871N → T.1 Publication
    Corresponds to variant rs10501429 [ dbSNP | Ensembl ].
    VAR_052636

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 227227Missing in isoform 2. CuratedVSP_054120Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP003086 Genomic DNA. No translation available.
    AP003110 Genomic DNA. No translation available.
    CH471076 Genomic DNA. Translation: EAW75061.1.
    BC007800 mRNA. Translation: AAH07800.2.
    CCDSiCCDS58164.1. [Q96I59-2]
    CCDS8261.1. [Q96I59-1]
    RefSeqiNP_001230180.1. NM_001243251.1. [Q96I59-2]
    NP_078954.4. NM_024678.5. [Q96I59-1]
    UniGeneiHs.503389.

    Genome annotation databases

    EnsembliENST00000281038; ENSP00000281038; ENSG00000137513. [Q96I59-1]
    ENST00000528850; ENSP00000432635; ENSG00000137513. [Q96I59-2]
    GeneIDi79731.
    KEGGihsa:79731.
    UCSCiuc001ozi.3. human. [Q96I59-1]

    Polymorphism databases

    DMDMi296452944.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP003086 Genomic DNA. No translation available.
    AP003110 Genomic DNA. No translation available.
    CH471076 Genomic DNA. Translation: EAW75061.1 .
    BC007800 mRNA. Translation: AAH07800.2 .
    CCDSi CCDS58164.1. [Q96I59-2 ]
    CCDS8261.1. [Q96I59-1 ]
    RefSeqi NP_001230180.1. NM_001243251.1. [Q96I59-2 ]
    NP_078954.4. NM_024678.5. [Q96I59-1 ]
    UniGenei Hs.503389.

    3D structure databases

    ProteinModelPortali Q96I59.
    SMRi Q96I59. Positions 41-472.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122846. 2 interactions.
    IntActi Q96I59. 1 interaction.
    STRINGi 9606.ENSP00000281038.

    Chemistry

    DrugBanki DB00174. L-Asparagine.

    PTM databases

    PhosphoSitei Q96I59.

    Polymorphism databases

    DMDMi 296452944.

    Proteomic databases

    MaxQBi Q96I59.
    PaxDbi Q96I59.
    PRIDEi Q96I59.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000281038 ; ENSP00000281038 ; ENSG00000137513 . [Q96I59-1 ]
    ENST00000528850 ; ENSP00000432635 ; ENSG00000137513 . [Q96I59-2 ]
    GeneIDi 79731.
    KEGGi hsa:79731.
    UCSCi uc001ozi.3. human. [Q96I59-1 ]

    Organism-specific databases

    CTDi 79731.
    GeneCardsi GC11M078147.
    HGNCi HGNC:26274. NARS2.
    HPAi HPA026793.
    MIMi 612803. gene.
    neXtProti NX_Q96I59.
    PharmGKBi PA143485554.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0017.
    HOGENOMi HOG000226033.
    HOVERGENi HBG067799.
    InParanoidi Q96I59.
    KOi K01893.
    OMAi LEDDMNC.
    OrthoDBi EOG7HF1J5.
    PhylomeDBi Q96I59.
    TreeFami TF315088.

    Enzyme and pathway databases

    Reactomei REACT_15302. Mitochondrial tRNA aminoacylation.

    Miscellaneous databases

    ChiTaRSi NARS2. human.
    GenomeRNAii 79731.
    NextBioi 69114.
    PROi Q96I59.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q96I59.
    Bgeei Q96I59.
    CleanExi HS_NARS2.
    Genevestigatori Q96I59.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    HAMAPi MF_00534. Asn_tRNA_synth.
    InterProi IPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004522. Asn-tRNA-ligase.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view ]
    PANTHERi PTHR22594. PTHR22594. 1 hit.
    PTHR22594:SF6. PTHR22594:SF6. 1 hit.
    Pfami PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view ]
    PRINTSi PR01042. TRNASYNTHASP.
    SUPFAMi SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00457. asnS. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-87.
      Tissue: Brain.
    4. "Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS."
      Bonnefond L., Fender A., Rudinger-Thirion J., Giege R., Florentz C., Sissler M.
      Biochemistry 44:4805-4816(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    5. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-353, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYNM_HUMAN
    AccessioniPrimary (citable) accession number: Q96I59
    Secondary accession number(s): G3V178
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 113 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3