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Protein

Protein phosphatase 1 regulatory subunit 16A

Gene

PPP1R16A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits protein phosphatase 1 activity toward phosphorylase, myosin light chain and myosin substrates.By similarity

GO - Molecular functioni

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 16A
Alternative name(s):
Myosin phosphatase-targeting subunit 3
Gene namesi
Name:PPP1R16A
Synonyms:MYPT3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:14941. PPP1R16A.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33634.

Polymorphism and mutation databases

BioMutaiPPP1R16A.
DMDMi22256976.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Protein phosphatase 1 regulatory subunit 16APRO_0000067040Add
BLAST
Propeptidei526 – 5283Removed in mature formSequence AnalysisPRO_0000396707

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi524 – 5241S-palmitoyl cysteineSequence Analysis
Modified residuei525 – 5251Cysteine methyl esterSequence Analysis
Lipidationi525 – 5251S-farnesyl cysteineSequence Analysis

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

MaxQBiQ96I34.
PaxDbiQ96I34.
PRIDEiQ96I34.

PTM databases

PhosphoSiteiQ96I34.

Expressioni

Gene expression databases

BgeeiQ96I34.
CleanExiHS_PPP1R16A.
ExpressionAtlasiQ96I34. baseline and differential.
GenevestigatoriQ96I34.

Organism-specific databases

HPAiHPA023755.

Interactioni

Subunit structurei

Binds PP1.

Binary interactionsi

WithEntry#Exp.IntActNotes
AESQ081173EBI-710402,EBI-717810
PPP1CBP621404EBI-710402,EBI-352350
PUF60Q9UHX12EBI-710402,EBI-1053259
TPP2P291443EBI-710402,EBI-1044672
TRIP6Q156543EBI-710402,EBI-742327

Protein-protein interaction databases

BioGridi124414. 19 interactions.
IntActiQ96I34. 19 interactions.
MINTiMINT-1368047.
STRINGi9606.ENSP00000292539.

Structurei

3D structure databases

ProteinModelPortaliQ96I34.
SMRiQ96I34. Positions 22-324.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati70 – 9930ANK 1Add
BLAST
Repeati103 – 13230ANK 2Add
BLAST
Repeati136 – 16530ANK 3Add
BLAST
Repeati231 – 26030ANK 4Add
BLAST
Repeati264 – 29330ANK 5Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili18 – 4528Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000236276.
HOVERGENiHBG053644.
InParanoidiQ96I34.
KOiK17458.
OMAiNARDSEC.
OrthoDBiEOG7P5T0H.
PhylomeDBiQ96I34.
TreeFamiTF316803.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017417. Pase-1_reg_su_16AB.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PIRSFiPIRSF038159. PP1_16AB_vert. 1 hit.
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96I34-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEHLELLAE MPMVGRMSTQ ERLKHAQKRR AQQVKMWAQA EKEAQGKKGP
60 70 80 90 100
GERPRKEAAS QGLLKQVLFP PSVVLLEAAA RNDLEEVRQF LGSGVSPDLA
110 120 130 140 150
NEDGLTALHQ CCIDDFREMV QQLLEAGANI NACDSECWTP LHAAATCGHL
160 170 180 190 200
HLVELLIASG ANLLAVNTDG NMPYDLCDDE QTLDCLETAM ADRGITQDSI
210 220 230 240 250
EAARAVPELR MLDDIRSRLQ AGADLHAPLD HGATLLHVAA ANGFSEAAAL
260 270 280 290 300
LLEHRASLSA KDQDGWEPLH AAAYWGQVPL VELLVAHGAD LNAKSLMDET
310 320 330 340 350
PLDVCGDEEV RAKLLELKHK HDALLRAQSR QRSLLRRRTS SAGSRGKVVR
360 370 380 390 400
RVSLTQRTDL YRKQHAQEAI VWQQPPPTSP EPPEDNDDRQ TGAELRPPPP
410 420 430 440 450
EEDNPEVVRP HNGRVGGSPV RHLYSKRLDR SVSYQLSPLD STTPHTLVHD
460 470 480 490 500
KAHHTLADLK RQRAAAKLQR PPPEGPESPE TAEPGLPGDT VTPQPDCGFR
510 520
AGGDPPLLKL TAPAVEAPVE RRPCCLLM
Length:528
Mass (Da):57,811
Last modified:December 1, 2001 - v1
Checksum:iA5A2D5E1A16A438C
GO

Sequence cautioni

The sequence BAC03452.1 differs from that shown.Cloning artifact.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 7138Missing in BAC03452 (Ref. 1) CuratedAdd
BLAST
Sequence conflicti249 – 2491A → P in BAC03452 (Ref. 1) Curated
Sequence conflicti272 – 2721A → P in BAC03452 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK090471 mRNA. Translation: BAC03452.1. Sequence problems.
BT006845 mRNA. Translation: AAP35491.1.
CH471162 Genomic DNA. Translation: EAW82080.1.
CH471162 Genomic DNA. Translation: EAW82081.1.
CH471162 Genomic DNA. Translation: EAW82082.1.
BC007854 mRNA. Translation: AAH07854.1.
BC049841 mRNA. Translation: AAH49841.1.
BC053506 mRNA. Translation: AAH53506.1.
CCDSiCCDS6429.1.
RefSeqiNP_116291.1. NM_032902.5.
XP_005272407.1. XM_005272350.2.
UniGeneiHs.521937.

Genome annotation databases

EnsembliENST00000292539; ENSP00000292539; ENSG00000160972.
ENST00000435887; ENSP00000391126; ENSG00000160972.
GeneIDi84988.
KEGGihsa:84988.
UCSCiuc003zdd.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK090471 mRNA. Translation: BAC03452.1. Sequence problems.
BT006845 mRNA. Translation: AAP35491.1.
CH471162 Genomic DNA. Translation: EAW82080.1.
CH471162 Genomic DNA. Translation: EAW82081.1.
CH471162 Genomic DNA. Translation: EAW82082.1.
BC007854 mRNA. Translation: AAH07854.1.
BC049841 mRNA. Translation: AAH49841.1.
BC053506 mRNA. Translation: AAH53506.1.
CCDSiCCDS6429.1.
RefSeqiNP_116291.1. NM_032902.5.
XP_005272407.1. XM_005272350.2.
UniGeneiHs.521937.

3D structure databases

ProteinModelPortaliQ96I34.
SMRiQ96I34. Positions 22-324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124414. 19 interactions.
IntActiQ96I34. 19 interactions.
MINTiMINT-1368047.
STRINGi9606.ENSP00000292539.

PTM databases

PhosphoSiteiQ96I34.

Polymorphism and mutation databases

BioMutaiPPP1R16A.
DMDMi22256976.

Proteomic databases

MaxQBiQ96I34.
PaxDbiQ96I34.
PRIDEiQ96I34.

Protocols and materials databases

DNASUi84988.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292539; ENSP00000292539; ENSG00000160972.
ENST00000435887; ENSP00000391126; ENSG00000160972.
GeneIDi84988.
KEGGihsa:84988.
UCSCiuc003zdd.3. human.

Organism-specific databases

CTDi84988.
GeneCardsiGC08P145703.
HGNCiHGNC:14941. PPP1R16A.
HPAiHPA023755.
MIMi609172. gene.
neXtProtiNX_Q96I34.
PharmGKBiPA33634.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000236276.
HOVERGENiHBG053644.
InParanoidiQ96I34.
KOiK17458.
OMAiNARDSEC.
OrthoDBiEOG7P5T0H.
PhylomeDBiQ96I34.
TreeFamiTF316803.

Miscellaneous databases

ChiTaRSiPPP1R16A. human.
GenomeRNAii84988.
NextBioi75568.
PROiQ96I34.
SOURCEiSearch...

Gene expression databases

BgeeiQ96I34.
CleanExiHS_PPP1R16A.
ExpressionAtlasiQ96I34. baseline and differential.
GenevestigatoriQ96I34.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017417. Pase-1_reg_su_16AB.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PIRSFiPIRSF038159. PP1_16AB_vert. 1 hit.
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The nucleotide sequence of a long cDNA clone isolated from human spleen."
    Jikuya H., Takano J., Kikuno R., Nagase T., Ohara O.
    Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung, Placenta and Uterus.

Entry informationi

Entry nameiPP16A_HUMAN
AccessioniPrimary (citable) accession number: Q96I34
Secondary accession number(s): D3DWM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: December 1, 2001
Last modified: May 27, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.