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Q96I34 (PP16A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 1 regulatory subunit 16A
Alternative name(s):
Myosin phosphatase-targeting subunit 3
Gene names
Name:PPP1R16A
Synonyms:MYPT3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length528 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits protein phosphatase 1 activity toward phosphorylase, myosin light chain and myosin substrates By similarity.

Subunit structure

Binds PP1.

Subcellular location

Cell membrane; Lipid-anchor By similarity.

Sequence similarities

Contains 5 ANK repeats.

Sequence caution

The sequence BAC03452.1 differs from that shown. Reason: Cloning artifact.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainANK repeat
Coiled coil
Repeat
   PTMLipoprotein
Methylation
Palmitate
Prenylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PUF60Q9UHX12EBI-710402,EBI-1053259
TRIP6Q156543EBI-710402,EBI-742327

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 525525Protein phosphatase 1 regulatory subunit 16A
PRO_0000067040
Propeptide526 – 5283Removed in mature form Potential
PRO_0000396707

Regions

Repeat70 – 9930ANK 1
Repeat103 – 13230ANK 2
Repeat136 – 16530ANK 3
Repeat231 – 26030ANK 4
Repeat264 – 29330ANK 5
Coiled coil18 – 4528 Potential

Amino acid modifications

Modified residue5251Cysteine methyl ester Potential
Lipidation5241S-palmitoyl cysteine Potential
Lipidation5251S-farnesyl cysteine Potential

Experimental info

Sequence conflict34 – 7138Missing in BAC03452. Ref.1
Sequence conflict2491A → P in BAC03452. Ref.1
Sequence conflict2721A → P in BAC03452. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q96I34 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: A5A2D5E1A16A438C

FASTA52857,811
        10         20         30         40         50         60 
MAEHLELLAE MPMVGRMSTQ ERLKHAQKRR AQQVKMWAQA EKEAQGKKGP GERPRKEAAS 

        70         80         90        100        110        120 
QGLLKQVLFP PSVVLLEAAA RNDLEEVRQF LGSGVSPDLA NEDGLTALHQ CCIDDFREMV 

       130        140        150        160        170        180 
QQLLEAGANI NACDSECWTP LHAAATCGHL HLVELLIASG ANLLAVNTDG NMPYDLCDDE 

       190        200        210        220        230        240 
QTLDCLETAM ADRGITQDSI EAARAVPELR MLDDIRSRLQ AGADLHAPLD HGATLLHVAA 

       250        260        270        280        290        300 
ANGFSEAAAL LLEHRASLSA KDQDGWEPLH AAAYWGQVPL VELLVAHGAD LNAKSLMDET 

       310        320        330        340        350        360 
PLDVCGDEEV RAKLLELKHK HDALLRAQSR QRSLLRRRTS SAGSRGKVVR RVSLTQRTDL 

       370        380        390        400        410        420 
YRKQHAQEAI VWQQPPPTSP EPPEDNDDRQ TGAELRPPPP EEDNPEVVRP HNGRVGGSPV 

       430        440        450        460        470        480 
RHLYSKRLDR SVSYQLSPLD STTPHTLVHD KAHHTLADLK RQRAAAKLQR PPPEGPESPE 

       490        500        510        520 
TAEPGLPGDT VTPQPDCGFR AGGDPPLLKL TAPAVEAPVE RRPCCLLM 

« Hide

References

[1]"The nucleotide sequence of a long cDNA clone isolated from human spleen."
Jikuya H., Takano J., Kikuno R., Nagase T., Ohara O.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Spleen.
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung, Placenta and Uterus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK090471 mRNA. Translation: BAC03452.1. Sequence problems.
BT006845 mRNA. Translation: AAP35491.1.
CH471162 Genomic DNA. Translation: EAW82080.1.
CH471162 Genomic DNA. Translation: EAW82081.1.
CH471162 Genomic DNA. Translation: EAW82082.1.
BC007854 mRNA. Translation: AAH07854.1.
BC049841 mRNA. Translation: AAH49841.1.
BC053506 mRNA. Translation: AAH53506.1.
RefSeqNP_116291.1. NM_032902.5.
XP_005272407.1. XM_005272350.1.
UniGeneHs.521937.

3D structure databases

ProteinModelPortalQ96I34.
SMRQ96I34. Positions 22-343.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124414. 14 interactions.
IntActQ96I34. 17 interactions.
MINTMINT-1368047.
STRING9606.ENSP00000292539.

PTM databases

PhosphoSiteQ96I34.

Polymorphism databases

DMDM22256976.

Proteomic databases

PaxDbQ96I34.
PRIDEQ96I34.

Protocols and materials databases

DNASU84988.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000292539; ENSP00000292539; ENSG00000160972.
ENST00000435887; ENSP00000391126; ENSG00000160972.
GeneID84988.
KEGGhsa:84988.
UCSCuc003zdd.3. human.

Organism-specific databases

CTD84988.
GeneCardsGC08P145703.
HGNCHGNC:14941. PPP1R16A.
HPAHPA023755.
MIM609172. gene.
neXtProtNX_Q96I34.
PharmGKBPA33634.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0666.
HOGENOMHOG000236276.
HOVERGENHBG053644.
InParanoidQ96I34.
KOK17458.
OMANARDSEC.
OrthoDBEOG7P5T0H.
PhylomeDBQ96I34.
TreeFamTF316803.

Gene expression databases

BgeeQ96I34.
CleanExHS_PPP1R16A.
GenevestigatorQ96I34.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017417. Pase-1_reg_su_16AB_euk.
[Graphical view]
PfamPF12796. Ank_2. 2 hits.
[Graphical view]
PIRSFPIRSF038159. PP1_16AB_vert. 1 hit.
PRINTSPR01415. ANKYRIN.
SMARTSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi84988.
NextBio75568.
PROQ96I34.
SOURCESearch...

Entry information

Entry namePP16A_HUMAN
AccessionPrimary (citable) accession number: Q96I34
Secondary accession number(s): D3DWM5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM