Q96I34 (PP16A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1 regulatory subunit 16A

Alternative name(s):
Myosin phosphatase-targeting subunit 3

Gene names

Name:	PPP1R16A
Synonyms:	MYPT3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits protein phosphatase 1 activity toward phosphorylase, myosin light chain and myosin substrates By similarity.
Subunit structure	Binds PP1.
Subcellular location	Cell membrane; Lipid-anchor By similarity.
Sequence similarities	Contains 5 ANK repeats.
Sequence caution	The sequence BAC03452.1 differs from that shown. Reason: Cloning artifact.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	ANK repeat Coiled coil Repeat
PTM	Lipoprotein Methylation Palmitate Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	plasma membrane Inferred from direct assay. Source: HPA
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PUF60	Q9UHX1	2	EBI-710402,EBI-1053259
TRIP6	Q15654	3	EBI-710402,EBI-742327

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Protein phosphatase 1 regulatory subunit 16A		PRO_0000067040
	Propeptide	526 – 528	3	Removed in mature form Potential		PRO_0000396707
Regions
	Repeat	70 – 99	30	ANK 1
	Repeat	103 – 132	30	ANK 2
	Repeat	136 – 165	30	ANK 3
	Repeat	231 – 260	30	ANK 4
	Repeat	264 – 293	30	ANK 5
	Coiled coil	18 – 45	28	Potential
Amino acid modifications
	Modified residue	434	1	Phosphotyrosine By similarity
	Modified residue	525	1	Cysteine methyl ester Potential
	Lipidation	524	1	S-palmitoyl cysteine Potential
	Lipidation	525	1	S-farnesyl cysteine Potential
Experimental info
	Sequence conflict	34 – 71	38	Missing in BAC03452. Ref.1
	Sequence conflict	249	1	A → P in BAC03452. Ref.1
	Sequence conflict	272	1	A → P in BAC03452. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q96I34 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: A5A2D5E1A16A438C	FASTA	528	57,811
	10 20 30 40 50 60 MAEHLELLAE MPMVGRMSTQ ERLKHAQKRR AQQVKMWAQA EKEAQGKKGP GERPRKEAAS 70 80 90 100 110 120 QGLLKQVLFP PSVVLLEAAA RNDLEEVRQF LGSGVSPDLA NEDGLTALHQ CCIDDFREMV 130 140 150 160 170 180 QQLLEAGANI NACDSECWTP LHAAATCGHL HLVELLIASG ANLLAVNTDG NMPYDLCDDE 190 200 210 220 230 240 QTLDCLETAM ADRGITQDSI EAARAVPELR MLDDIRSRLQ AGADLHAPLD HGATLLHVAA 250 260 270 280 290 300 ANGFSEAAAL LLEHRASLSA KDQDGWEPLH AAAYWGQVPL VELLVAHGAD LNAKSLMDET 310 320 330 340 350 360 PLDVCGDEEV RAKLLELKHK HDALLRAQSR QRSLLRRRTS SAGSRGKVVR RVSLTQRTDL 370 380 390 400 410 420 YRKQHAQEAI VWQQPPPTSP EPPEDNDDRQ TGAELRPPPP EEDNPEVVRP HNGRVGGSPV 430 440 450 460 470 480 RHLYSKRLDR SVSYQLSPLD STTPHTLVHD KAHHTLADLK RQRAAAKLQR PPPEGPESPE 490 500 510 520 TAEPGLPGDT VTPQPDCGFR AGGDPPLLKL TAPAVEAPVE RRPCCLLM

References

[1]	"The nucleotide sequence of a long cDNA clone isolated from human spleen." Jikuya H., Takano J., Kikuno R., Nagase T., Ohara O. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung, Placenta and Uterus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK090471 mRNA. Translation: BAC03452.1. Sequence problems. BT006845 mRNA. Translation: AAP35491.1. CH471162 Genomic DNA. Translation: EAW82080.1. CH471162 Genomic DNA. Translation: EAW82081.1. CH471162 Genomic DNA. Translation: EAW82082.1. BC007854 mRNA. Translation: AAH07854.1. BC049841 mRNA. Translation: AAH49841.1. BC053506 mRNA. Translation: AAH53506.1.
IPI	IPI00063273.
RefSeq	NP_116291.1. NM_032902.5.
UniGene	Hs.521937.
3D structure databases
ProteinModelPortal	Q96I34.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96I34. 15 interactions.
MINT	MINT-1368047.
STRING	9606.ENSP00000292539.
PTM databases
PhosphoSite	Q96I34.
Polymorphism databases
DMDM	22256976.
Proteomic databases
PaxDb	Q96I34.
PRIDE	Q96I34.
Protocols and materials databases
DNASU	84988.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000292539; ENSP00000292539; ENSG00000160972. ENST00000435887; ENSP00000391126; ENSG00000160972.
GeneID	84988.
KEGG	hsa:84988.
UCSC	uc003zdd.3. human.
Organism-specific databases
CTD	84988.
GeneCards	GC08P145703.
HGNC	HGNC:14941. PPP1R16A.
HPA	HPA023755.
MIM	609172. gene.
neXtProt	NX_Q96I34.
PharmGKB	PA33634.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0666.
HOGENOM	HOG000236276.
HOVERGEN	HBG053644.
InParanoid	Q96I34.
OMA	NARDSEC.
OrthoDB	EOG434W62.
PhylomeDB	Q96I34.
Gene expression databases
Bgee	Q96I34.
CleanEx	HS_PPP1R16A.
Genevestigator	Q96I34.
GermOnline	ENSG00000160972. Homo sapiens.
Family and domain databases
Gene3D	1.25.40.20. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR017417. Pase-1_reg_su_16AB_euk. [Graphical view]
Pfam	PF12796. Ank_2. 2 hits. [Graphical view]
PIRSF	PIRSF038159. PP1_16AB_vert. 1 hit.
PRINTS	PR01415. ANKYRIN.
SMART	SM00248. ANK. 4 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 4 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	84988.
NextBio	75568.
SOURCE	Search...

Entry information

Entry name

PP16A_HUMAN

Accession

Primary (citable) accession number: Q96I34
Secondary accession number(s): D3DWM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 2002
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.